Kitasamycin monoclonal antibody hybridoma cell strain SML and application thereof

A hybridoma cell line, monoclonal antibody technology, applied in the preparation of hybrid peptides, sugar derivatives, animal/human proteins, etc. The effect of detection sensitivity
CN110257342AInactive Publication Date: 2019-09-20JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-09-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a Kitasamycin monoclonal antibody hybridoma cell strain SML and an application thereof, and belongs to the field of food safety immune detection. The hybridoma cell line SML is deposited in the general microbiology center of the Council for the preservation and administration of microbial strains in China, with the preservation number of CGMCC No. 17392. The complete antigen of kitasamycin and equivalent freund's adjuvant are mixed and emulsified to immunize BALB / c rats. Complete freund's adjuvant is used for the first immunization, incomplete freund's adjuvant is used for several times to strengthen immunization, and finally, kitasamycin complete antigen is used for sprint immunization. Splenic cells of rats with high titer and low IC50 are fused with myeloma cells of rats by a PEG method, cultured and screened; then the cells are screened by indirect competitive enzyme-linked immunosorbent assay and subcloned three times to finally obtain a monoclonal antibody hybridoma cell strain. The monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity to kitasamycin, can realize the detection of kitasamycin residues in aquatic products, and has practical application value.
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Description

technical field

[0001] The invention relates to a kitasarmycin monoclonal antibody hybridoma cell line SML and an application thereof, belonging to the field of food safety immunoassay. Background technique

[0002] Kitamycin (KIT) is a multi-component macrolide antibiotic produced by Streptomyces. The antibacterial performance is similar to that of erythromycin, and it has a strong inhibitory effect on Gram-positive bacteria, such as Staphylococcus, Streptococcus pyogenes, Streptococcus viridans, Streptococcus pneumoniae, Bacillus tetanus, and Bacillus diphtheria. It is used to prevent bacterial infection and mycoplasma disease in livestock and poultry, as well as as a growth-promoting agent. KIT is a fat-soluble drug that will remain in the environment for a long time, causing water and soil pollution, and may enter the human body through the food chain, causing damage to human organs to a certain extent, and may even lead to the emergence of drug-resistant bacteria. Man...

Claims

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