6 results about "Freund's adjuvant" patented technology

The invention relates to the field of vaccines, in particular to application of a bacillus calmette-guerin vaccine and a complete freund's adjuvant in preparation of tuberculosis prevention vaccines. The invention provides a novel inoculation strategy of subcutaneous combined inoculation of the bacillus calmette guerin vaccine and the complete freund's adjuvant, the safety consistent with that of the existing tuberculosis prevention vaccine in the clinical stage is achieved, and the immune protection effect higher than that of the existing tuberculosis prevention vaccine in the clinical stage and the preclinical research stage is achieved. The compound is expected to be used as a novel tuberculosis vaccine for preventing tuberculosis.

The invention provides a method for constructing a myositis-related interstitial lung disease mouse model, which comprises the following steps: injecting 10 micrograms of ODN2395 into the abdominal cavity of a female BALB / c mouse, injecting 0.25 mL of a mixed emulsion of SD (Sprague Dawley) rat skeletal muscle homogenate and a complete Freund's adjuvant into each of the two sides of the back of the female BALB / c mouse at the same time point, and feeding for a certain time to obtain a film-formed mouse. The method has the advantages that raw materials are easy to obtain, and the modeling method is simple; 2, the modeling time is relatively short; and 3, the model forming rate is high, and the method is more in line with the pathogenesis and pathological phenotype of IIM-ILD.

The utility model discloses a device for promoting full mixing of type II collagen and a freund's adjuvant. The device comprises a container, an injector and a connecting pipe, holes are formed in the two sides of the container, and a low-temperature medium can be injected into the container. The tube bodies of the injectors are arranged inside the container through the hole sites, the operating ends of the injectors are arranged outside the container, and the two injectors are connected through the connecting tube; the outer wall of the tube body of the injector and the tube wall of the connecting tube are coated or wound with a shading material, or the outer wall of the container is coated with a shading material. The syringes are arranged in the holes in the two sides of the container, the low-temperature medium is injected into the container, the outer walls of the tube bodies of the syringes and the tube wall of the connecting tube are coated or wound with the shading materials, or the outer wall of the container is coated with the shading materials, and therefore a low-temperature shading environment is provided to promote fusion.

The invention belongs to the technical field of medicines, and relates to a Chinese tamarisk twig alcohol extract with anti-inflammatory activity as well as a preparation method and application thereof, and the Chinese tamarisk twig alcohol extract is one or more of 50% ethanol total extract, 20% ethanol subpart and 40% ethanol subpart of Chinese tamarisk twig. The Chinese tamarisk twig alcohol extract also has any one of the following applications: preparation of a medicine for preventing and treating inflammation in aquaculture, preparation of a medicine for preventing and / or treating fish inflammation, and preparation of a feed in aquaculture. The 50% ethanol extract prepared by the invention has a good improvement effect on a lipopolysaccharide (LPS)-induced zebrafish inflammation model, and has a remarkable treatment effect on rats with rheumatoid arthritis induced by a complete Freund's adjuvant; two new active parts are further prepared, the activity of the active parts is superior to that of total extracts, and the active parts have a remarkable improvement effect on LPS-induced Tg (mpx: EGFP) transgenic zebrafish inflammation and can be used for being developed into new anti-inflammatory traditional Chinese medicines in the future.

The application provides a vaccine against ichthyophthiriasis and a preparation method and application thereof, and belongs to the technical field of fish disease prevention and control. The application uses inactivated whole worms of in-vitro large-scale cultured Amphilina alata as a cross antigen, and fish complement C5a protein as an immunoadjuvant, and the two synergistically play a high-efficiency immune protection role. The application mixes and emulsifies the C5a adjuvant and the antigen at an effective dose of 1x10 4 6x10 4 per tail to prepare the vaccine. After intraperitoneal injection and secondary boosting, the vaccine produces significant cross protection against the infection of the ichthyophthirius, and the protection effect is significantly better than that of a traditional Freund's adjuvant vaccine. The application breaks the bottleneck that the ichthyophthirius cannot be cultured in vitro and thus a large number of worm bodies cannot be obtained for large-scale preparation of whole worm vaccines, and also solves the problem of poor safety of traditional adjuvants, has the advantages of low cost, good effect, easy large-scale production and the like, and is suitable for green immune prevention and control of ichthyophthiriasis of freshwater fish.

The application discloses a kind of phenylboronic acid and dopamine grafted chitosan-manganese-based injectable hydrogel immunoadjuvant and its preparation method and application.The hydrogel takes chitosan as base material, constructs modified polymer by chemical grafting dopamine and phenylboronic acid;Manganese ion is loaded to form phenylboronic acid and dopamine grafted chitosan-manganese-based injectable hydrogel immunoadjuvant.The hydrogel is fixed antigen by physical adsorption, has the functions of targeted delivery, immune activation and controllable release, significantly enhances humoral immunity and cellular immunity.The hydrogel has the characteristics of low viscosity, supports simple injection, biodegradable and no local inflammatory reaction.Experiments show that the titer of the adjuvant is equivalent to that of Freund's adjuvant after immunizing mice, successfully used for preparing monoclonal antibody of gladiolus burkholderia, and constructs immune test strip, and the sensitivity reaches 5×10 5 CFU / mL.The application solves the problems of high toxicity, local damage and high cost of traditional adjuvant, and is suitable for vaccine development and immunodiagnosis.