Preparation method of anti-idiotypic egg yolk antibody

Abstract

The invention discloses a preparation method of an anti-idiotypic egg yolk antibody and relates to the technical field of antibody preparation. The preparation method comprises the steps as follows: preparing an immunogen; mixing the immunogen with an incomplete Freund's adjuvant, after uniform mixing, and performing subcutaneous injection immunization on a mouse according to a first preset condition to obtain an anti-antigen antibody of the mouse; after mixing the anti-antigen antibody of the mouse with the incomplete Freund's adjuvant, injecting the mixture to immunize laying hens for presettimes according to a second preset condition; acquiring eggs laid by laying hens the same day; collecting high-immunity eggs according to a fourth preset condition; performing yolk separation, preservation and crushing of yolk tissue on high-immunity eggs; after treating yolk liquid, collecting filtrate to obtain a crude product of the anti-idiotypic egg yolk antibody; purifying the crude productof the anti-idiotypic egg yolk antibody; and conducting titer detection, specific detection and competitive inhibition tests on the anti-idiotypic egg yolk antibody separately.

Description

technical field [0001] The invention belongs to the technical field of antibody preparation, and in particular relates to a method for preparing an anti-idiotype egg yolk antibody. Background technique [0002] At present, anti-idiotypic monoclonal antibodies are more commonly used in the preparation of anti-idiotypic antibodies. In this method, the monoclonal antibodies of the antigen are first prepared, and the monoclonal antibodies that have immune neutralization effect on the antigen function are screened out, and then the monoclonal antibodies are used Immunizing mice again to prepare anti-idiotypic monoclonal antibodies involves two times of cell fusion to prepare two hybridoma cell lines, which is time-consuming and labor-intensive. [0003] Moreover, it takes at least one year to prepare a useful anti-idiotypic monoclonal antibody, and the research and development process is long and costly. At the same time, there is also anti-idiotypic polyclonal antibody preparat...

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Problems solved by technology

[0004] The embodiment of the present application provides a preparation method of anti-idiotypic yolk antibody, which solves the problem of the long development cycle and high cost of anti-idiotypic monoclonal antibody in the prior art, and the anti-idiotype rabbit antibody needs to kill a large number of animals, and it is difficult to carry out non-idiotypic monoclonal antibodies. Bacteria treatment, technical problems that are not suitable for industrialized production, achieved the technical effects of shortening the R&D and production cycle, saving time and effort, saving costs, using various ways, not requiring mass killing, rich in resources, easy to do aseptic treatment, and industrialized production

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