ETEC (enterotoxigenic escherichla coli) yolk antibody powder and preparation method thereof

Abstract

The invention discloses ETEC (enterotoxigenic escherichla coli) yolk antibody powder and a preparation method thereof. The method comprises following steps: escherichia coli strains are cultured in a solid culture medium at 35-40 DEG C for 18-24 h; after culture, pilin is extracted coarsely from the solid culture medium and is purified; the purified pilin and equal volume of a freund's adjuvant are mixed, an oil-emulsion vaccine is formed and used for immunizing a primiparous hen, and serum and a yolk antibody are collected after immunization; the yolk antibody is subjected to spray drying through a powder sprayer, and the yolk antibody powder is prepared. Analysis of the titer of the immunized yolk antibody with ELISA (enzyme-linked immunosorbent assay) discovers that higher-titer yolk antibody can be obtained by using purified pilin as an antigen for immunization. After the yolk antibody powder is prepared from the yolk antibody through spray drying by the powder sprayer, the antibody titer is only reduced by one degree of multiple proportions, the yolk antibody has very good high-temperature stability, and a power preparation process adopting a spray drying method is applicable to production of the yolk antibody powder.

Description

technical field [0001] The invention relates to the field of food processing, in particular to an ETEC yolk antibody powder and a preparation method thereof. Background technique [0002] Piglet diarrhea is a typical multifactorial disease that plagues the pig industry and brings huge economic losses to the pig industry. Piglet diarrhea caused by Escherichia coli is one of the more serious hazards. The main pathogen of Escherichia coli diarrhea in piglets is Enterotoxigenic Escherichlacoli (ETEC). The pathogenic effect of ETEC is mainly composed of two types of virulence factors such as enterotoxin and adhesin pili. The main pathogenic link is ETEC binds to specific receptors on the epithelial cells of the small intestinal mucosa through the adhesin pili, and then proliferates and produces a large amount of toxins, causing abnormal absorption and secretion of the small intestine, resulting in diarrhea. Obtaining antibodies through breast milk can better prevent piglets from...

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a kind of ETEC egg yolk antibody powder and its preparation method, aiming at solving the problem that the existing antibodies will disappear soon after weaning, high immune serum is expensive, and antibiotic treatment often causes bacteria The problem of drug resistance

Images