173 results about "Titin Antibody" patented technology

The invention discloses a method for detecting the valence of an antibody. The method comprises the following steps: 1), coupling an antigen on a microsphere to obtain a modified microsphere; 2), mixing and reacting the modified microsphere with an antibody solution; 3), measuring the turbidity of the reaction liquid, so as to determine the valence of the antibody. According to the method provided by the invention, CCP is modified on the surface of a polystyrene microsphere by adopting a vitamin H-streptavidin system, so as to prepare a granular CCP antigen successfully; in case that the granular CCP antigen prepared by adopting the method is used for detecting the valence of anti-CCP antibody in blood serum, the linearity of the detecting result is good, and the detecting process can be completed by 20 minutes, so that the method can be used for acquiring the detecting result more quickly as compared with the conventional ELISA detecting method consuming 2-3 hours.

The invention relates to a hand-foot-and-mouth disease resistant human immunoglobulin, and preparation and using methods and application thereof in pharmacy. The preparation method comprises the following steps of: separating component I+II+III, I+III and II precipitates in turn by using a low-temperature methanol protein separation method; performing pasteurization on a component II precipitate; refining and purifying; performing dealcholization; preparing; and sterilizing, packaging and performing low-pH incubated inactivation. The product has antibody titer of not less than 1:640 for enteroviruses (including one or more of coxsackie virus, Echo and EV71), the immunoglobulin content is not less than 95.0 percent of the total protein content, and the sum of IgG monomer and dimer is not less than 95 percent; and the using method is that a specific antibody with titer of 160,000-320,000 is intravenously infused. The invention is suitable for industrial production; and the product has high-titer enterovirus resistant specificity, is safe and reliable, and can become an effective medicine for treating the hand-foot-and-mouth disease.

The invention discloses an ELISA kit of IBDV antibodies, a test method and an effective antibody titer determination method. The ELISA kit comprises (1) an enzyme-labeled board coated with IBDV antigens and (2) goat anti-chicken Ig Y diluted according to the ratio of 1:3,000 and marked with HRP, wherein the enzyme-labeled board coated with the IBDV antigens is made by diluting the concentration ofthe IBDV antigens to 10ng / pore and then coating corresponding pores in a reaction board with diluent, the IBDV antigens are rVP2 proteins prepared through a baculovirus expression system, and a detection result is judged according to an S / P value obtained through an ELISA detection method. When the detection result is positive according to the S / P value obtained through the ELISA detection method, it is indicated that effective antibodies are in a high level and can neutralize IBDVs, and therefore it is needless to perform vaccine immunization again; and when the detection result is negativeaccording to the S / P value, it is indicated that the antibodies are in a low level without protective force and are prone to being infected by viruses, and therefore it is needed to perform vaccine immunization again. As a result, the established ELISA kit has a high antibody positivity detection rate and can guide vaccine immunization on production to reduce economic losses.

The invention relates to the technical field of biology, and discloses a dimethomorph hapten, and a preparation method and an application thereof. The dimethomorph hapten reserves the chemical structure of dimethomorph to the greatest extent, a nitrogen-containing group capable of being coupled with proteins is introduced through chemical synthesis, the synthesis method is simple, and the productpurity and yield are high; and the hapten is used as a raw material to prepare an antigen system suitable for animal immunization, and a prepared monoclonal antibody has good specificity, affinity and antibody titer, and can be used for preparing an enzyme linked immunosorbent assay kit, and the kit has the advantages of low detection cost, and fast, efficient and accurate detection method, and is suitable for on-site monitoring of dimethomorph residues in vegetables and fruits and screening of a large number of samples.

The invention relates to an anti-cryptococcal capsular polysaccharide hybridoma cell strain and an anti-cryptococcal capsular polysaccharide monoclonal antibody secreted thereof. The antibody can be specifically bound to cryptococcal capsular polysaccharide and can be used for in vitro detection of cryptococcal infection. The antibody titer is up to more than one million, and the antibody has excellent affinity and good specific binding capability. The detection limit of a colloidal gold-labeled immunodiagnostic reagent developed with the antibody as the raw material is 0.5 ng / ml, and the antibody has excellent performance in sensitivity, specificity, stability and all other aspects.

The invention belongs to the technical field of immunoassay, and concretely relates to a method for efficiently preparing an Enterobacter sakazakii polyclonal antibody. The method comprises the following steps: immunizing New Zealand white rabbits with Enterobacter sakazakii granular antigens through adopting a subcutaneous multi-point injection and ear vein venous injection combination technology, and further purifying antiserum by a Protein A affinity chromatography column to rapidly obtain the high-tilter Enterobacter sakazakii polyclonal antibody. Compared with traditional immunization methods, the method disclosed in the invention has the advantages of shortening of the immunization time by 7-14d, and great increase of the antibody tilter. A West-blotting and IFAT combination technology is used to detect the specificity of the antibody, and a result shows that the prepared antibody has very high specificity to Enterobacter sakazakii. The method provided by the invention is simple and efficient, provides technical support for preparation of the Enterobacter sakazakii polyclonal antibody, and is helpful for developing the immunodetection technology of the Enterobacter sakazakii.

The invention discloses a specific egg-yolk antibody for preventing and treating feline distemper. The specific egg-yolk antibody is prepared by taking FPV inactivated vaccine and FPV subunit vaccineas immunogens and using immune laying hens. Accordingly, a corresponding preparation method is established and comprises the following steps: creatively inoculating laying hens with two different FPVimmunogens, taking the FPV inactivated vaccine as a basic immunogen and the FPV subunit vaccine as a strengthened immunogen; optimizing an immunization method and inoculation dose to produce high antibody titer; immunizing the laying hens, then harvesting eggs from hype-immunized chickens, separating egg yolk, and extracting and purifying a coarse product of the yolk antibody, thus obtaining the specific egg-yolk antibody for preventing and treating the feline distemper. The test shows that the yolk antibody disclosed by the invention has the advantages of good safety, high specificity, high antibody titer, low cost and high yield; the specific egg-yolk antibody can be used for treatment and emergency prevention of the feline distemper and enhancing the resistance of sick cats and has a good popularization and application prospect.

The invention relates to a monoclonal antibody hybridoma cell line 1H6 for resisting infectious bursal disease virus VP2 protein, and belongs to the technical field of biology. In the invention, BALB / c mice are immunized with purified IBDV QL strain antigen, splenocytes are prepared and fused to an SP2 / 0 myeloma cell line, double screening is carried out by IBDV recombinant VP2 (rVP2) protein expressed by prokaryote and purified IBDV QL strain to obtain the monoclonal antibody hybridoma cell line 1H6 which not only can react with rVP2 protein expressed by prokaryote, but also can react with IBDV, the antibody subclass is IgG1 kappa, and the antibody titers of induced ascites are 108 respectively. Sandwich ELISA assay shows that 1H6 has no cross-reaction with other four avian viruses; indirect immunofluorescence test proves that 1H6 has good specific reaction; and immunoblot analysis shows that 1H6 can produce specific protein bands with rVP2 protein and IBDV.