Method for producing antibody by coupling multi-polypeptide epitope of protein antigen with carrier

Abstract

The invention relates to a method for producing an antibody by coupling multi-polypeptide epitope of protein antigen with a carrier and solves the problems such as high production cost, difficulty in purification, or long period, complex preparation technology and low valence in the existing antibody producing method. The antibody is obtained by selection and synthesization of epitope, coupling of various polypeptides with the carrier, animal immunology and detection, and an antigen affinity chromatography extraction method. The method has the advantages of being simple, low in cost, short in period and high in valence of the prepared antibody.

Description

technical field [0001] The invention belongs to the technical field of polyclonal antibody production of protein antigen, and specifically relates to a method for producing antibody by coupling multiple polypeptide antigen epitopes of protein antigen to a carrier. Background technique [0002] Antibody is an important tool in immunological detection. Its main preparation method is to stimulate the body to produce immunoglobulin (referred to as antibody) by immunizing animals with antigens, and then obtain antibodies by extraction and purification for immunological detection or crop therapeutic drugs. Antigen production methods mainly include artificial extraction of natural antigens and artificial synthesis of complete antigens. Artificially extracted natural antigens have a good natural three-dimensional conformation, which can better stimulate the animal body to produce an immune response. The obtained antibodies have the advantages of high titer and high affinity. It is ...

AI Technical Summary

Problems solved by technology

Artificially extracted natural antigens have a good natural three-dimensional conformation, which can better stimulate the animal body to produce an immune response. The obtained antibodies have the advantages of high titer and high affinity. It is extremely difficult and the purification cost is high, so it is difficult to effectively promote the artificial extraction of natural antigens; at present, the artificial synthesis of complete antigens, especially protein antigens, is mainly achieved through peptide synthesis or recombinant proteins. The technology of recombinant proteins is still restricted by technology. The success rate of expression is not high, only 20% or even lower; the single peptide synthesis technology has a short peptide chain, resulting in poor immunogenicity, and due to the diversity of protein sequences, it is easy to repeat sequences with other proteins, resulting in production Antibody specificity is not good

In addition, there is another genetic engineering immunization method. Since the genetic engineering immunization method is rarely used in the preparation of antibodies, and requires the use of molecular biology platforms, and has a low success rate and low antibody titer, it is currently only in the laboratory stage.

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