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Haemophilus parasuis outer membrane protein P5 (OMP5) resistant monoclonal antibody, hybridoma cell strain and application

A hybridoma cell line, the technology of Haemophilus suis, is applied in the field of immunology to achieve the effect of high titer and strong versatility

Inactive Publication Date: 2013-01-16
广东省农业科学院兽医研究所
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AI Technical Summary

Problems solved by technology

At present, there is no commercially available diagnostic method for antibody-based HPS detection in China

Method used

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  • Haemophilus parasuis outer membrane protein P5 (OMP5) resistant monoclonal antibody, hybridoma cell strain and application
  • Haemophilus parasuis outer membrane protein P5 (OMP5) resistant monoclonal antibody, hybridoma cell strain and application
  • Haemophilus parasuis outer membrane protein P5 (OMP5) resistant monoclonal antibody, hybridoma cell strain and application

Examples

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Embodiment Construction

[0025] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Without departing from the spirit and essence of the present invention, any modifications or substitutions made to the methods, steps or conditions of the present invention fall within the scope of the present invention.

[0026] Unless otherwise specified, the chemical reagents used in the examples are all conventional commercially available reagents, and the technical means used in the examples are conventional means well known to those skilled in the art.

[0027] Main experimental materials and sources:

[0028] Actinobacillus pleuropneumoniae type 1 (APP259, purchased from China Veterinary Drug Administration); HPS type 3 strain (SW114, gifted by Dr. Pat Blackall, Queensland Animal Health Research Institute, Australia); HPS type 11 (H456, Queensland Animal Health, Australia Institute Dr. Pat Blackall); Escherichia coli, Pasteurel...

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Abstract

The invention discloses a haemophilus parasuis outer membrane protein P5 (OMP5) resistant monoclonal antibody, a hybridoma cell strain and an application. The hybridoma cell strain is preserved in the China center for type culture collection (CCTCC), and the preservation serial number is CCTCCC2012135. The monoclonal antibody prepared by the hybridoma cell strain is good in specificity, high in valence, high in generality, free from cross reaction with swine Escherichia coli, swine pasteurella, swine pleuropneumonia actinobacillus, streptococcus suis and swine bordetella bacilli, capable of detecting haemophilus parasuis with different serotypes and widely applicable to etiology diagnosis, serology detection and immunology detection and prevention of haemophilus parasuis diseases, and the enzyme-linked immuno sorbent assay (ELISA) antibody valence can reach 1:204800 after purification.

Description

technical field [0001] The invention belongs to the field of immunology, and in particular relates to a monoclonal antibody against the outer membrane protein OMP5 of Haemophilus parasuis, a hybridoma cell line and applications thereof. Background technique [0002] Haemophilus parasuis ( Haemophilus Parasuis , HPS) is a non-motile, small, NAD-dependent, polymorphic Gram-negative bacillus belonging to the Pasteurellaceae family and the genus Haemophilus. HPS is a common bacterium that usually colonizes the upper respiratory tract of pigs and can often be isolated in the nasal cavity, tonsils, and anterior trachea without causing any clinical symptoms. HPS is also the causative agent of Glasser's disease in pigs. Under certain conditions, HPS can cause a systemic infection characterized by fibrinous polyserositis, arthritis, and meningitis. Glaser's disease has become the main bacterial disease affecting the swine industry worldwide, causing huge losses to the swine indust...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/12G01N33/577C12R1/91
Inventor 李春玲
Owner 广东省农业科学院兽医研究所
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