The invention belongs to the field of
biomedicine, providing a method for detecting animal pathogenic microorganisms and a special
protein chip thereof. The detecting method essentially comprises the following steps of: a. preparing the
protein chip, selecting a plurality of different groups of specific
antigen of many kinds of pathogenic microorganisms by means of an experiment to be fixed on a properly modified carrier; and b. detecting: reacting
blood serum of a animal to be detected with the
protein chip, hybridizing with a marked secondary
antibody, detecting a
hybrid positive
signal in a proper detection way, and obtaining a
hybrid result of the
blood serum and the chip by means of scanning and analyzing, wherein the
blood serum infected with the different pathogenic microorganisms can show different positive
signal combination
modes after the reaction, so as to ensure whether the animal to be detected is infecting or is infected with the pathogenic microorganisms marked on the
protein chip. The invention can simultaneously detect many kinds of the pathogenic microorganisms, improves detecting efficiency, selects many groups of different
antigen with a certain specificity aiming at each
pathogenic microorganism, leads the detection to have good specificity, and has good application prospect in detecting the animal pathogenic microorganisms.