7 results about "Serum reaction" patented technology

The invention discloses an intelligent pulmonary tuberculosis detection method and system based on images and clinical data, and belongs to the field of medicines.The method comprises the steps that S1, high-resolution chest X-ray images and clinical data such as serum C-reactive protein concentration, erythrocyte sedimentation value, erythrocyte distribution width and body temperature are collected; s2, performing non-linear contrast enhancement on the image, and extracting lung gate texture, lung field transparency and cavity edge features; s3, fusing the image and the clinical features through a graph attention mechanism, and generating a high-dimensional fusion vector; s4, risk grade discrimination is carried out through a multi-scale residual mapping network, and five-grade labels are output; and S5, optimizing the model by utilizing the pathology definite diagnosis sample in combination with the cross entropy and the center loss. The method has the beneficial effects that accurate grading evaluation of the pulmonary tuberculosis risk is realized, and the accuracy of early screening and the clinical interpretability of the model are improved.

The invention discloses a recombinant antigen protein, a recombinant vector, a recombinant host cell, a virus-like particle and application thereof and a vaccine, and relates to the technical field of antigen preparation, the recombinant antigen protein comprises an icosahedral porcine circovirus capsid protein and an A104R protein loaded on the surface of the icosahedral porcine circovirus capsid protein, the A104R protein comprises a protein coded by an African swine fever virus A104R gene. The recombinant antigen protein capable of simultaneously reacting with positive serum of the African swine fever virus and positive serum of the porcine circovirus is prepared, and the vaccine prepared by mixing the recombinant antigen protein with an adjuvant can effectively stimulate a pig body to generate specific antibodies of the African swine fever virus and the porcine circovirus; therefore, reference is provided for joint defense of the two viruses. The recombinant antigen protein provided by the invention has the characteristics of stable expression, high yield and high solubility.

The invention discloses a hybridoma cell strain based on type 1 goose astrovirus, a construction method, a monoclonal antibody and a detection reagent. The hybridoma cell strain is a hybridoma cell strain A5A1, the preservation number of the hybridoma cell strain is CCTCC (China Center for Type Culture Collection) NO: C202505, and the hybridoma cell strain is preserved in the China Center for Type Culture Collection on March 26, 2025. According to the invention, a mouse is immunized by GAstV-1ORF2 recombinant protein expressed by a prokaryotic expression system, and a monoclonal antibody A5A1 is obtained by screening and subcloning. Indirect competitive ELISA (icELISA) for detecting the GAstV-1 antibody is developed based on the hybridoma cell strain A5A1, and the method is high in sensitivity, good in repeatability and high in specificity, only reacts with serum of GAstV-1, and does not have cross reaction with other detected pathogenic serum. A serological detection method established by the monoclonal antibody prepared by the method lays a foundation for scientific prevention and control of gout of goslings.

The present disclosure includes compositions and methods for treating cardiovascular disease (CVD) (e.g., cholesterol related disorders, or diseases caused or characterized by increased levels of plasma triglycerides, plasma cholesterol, or serum C-reactive protein), or symptoms thereof. The disclosure also includes compositions and methods for lowering plasma triglycerides in a subject, lowering plasma cholesterol levels in a subject, and lowering serum C-reactive protein levels in a subject.

The utility model relates to the technical field of blood collectors, in particular to a blood collector with a blood type detection function. The following technical scheme is provided mainly aiming at the problems that a traditional serum-blood mixed blood type detection method is commonly adopted in a blood donation site at present, so that the pain and the contradiction emotion of a blood donor are increased due to repeated puncture blood sampling, the waiting time of serum reaction is long, and the working efficiency and the satisfaction degree of the blood donor are reduced, and the device comprises a collection box and a cover plate covering the collection box, a liquid inlet pipe is mounted at the top of the cover plate, and a liquid discharge pipe is connected to the bottom of the collecting box; the detection mechanism is mounted on one side of the collection box and used for detecting the blood type of the blood sucked into the collection box. According to the blood collector, blood type detection and blood collection can be completed synchronously, the pain and the contradiction emotion of a blood donor are relieved, meanwhile, waste of time for waiting for serum reaction is eliminated, the work efficiency of a blood donation site and the satisfaction degree of the blood donor are improved, and smooth blood donation work is powerfully promoted.

The invention is applicable to the technical field of biology, and provides screening of pregnant bovine serum aptamers based on a magnetic-SELEX technology and application of the pregnant bovine serum aptamers. Based on a magnetic-SELEX (systematic evolution of ligands by exponential enrichment) technology, cow early pregnancy related aptamers are screened by taking pregnant cow serum 21d after fertilization as a target, and homology analysis and secondary structure prediction are performed on the aptamers. The aptamer reacts with negative and positive serum of pregnant cattle, the optimal expression of the L4 aptamer is determined according to the P / N value, and the detection conditions are optimized. The L4 aptamer is used for actual sample detection, the result shows that the L4 aptamer shows high specificity in early pregnancy 14-21 d detection, the OD450 nm value of a positive sample is obviously higher than that of a negative sample, the detection effect is obviously superior to that of a commercial kit, a detection window is advanced to 14 d, and the L4 aptamer has important clinical significance in early pregnancy diagnosis of dairy cows and has good application prospects in early pregnancy diagnosis of dairy cows. A novel tool with low cost and high sensitivity is provided for intensive breeding of dairy cows, and large-scale development of animal husbandry is further promoted.