Specific protein reaction detection method and device

Abstract

A specific protein reaction detection method comprises the following steps: respectively carrying out C-reactive protein detection on a serum sample, a plasma sample and a blood sample of the same collection source to obtain specific protein reaction curves of the serum sample, the plasma sample and the blood sample, respectively acquiring curve characteristics of the serum sample, the plasma sample and the blood sample and inputting the curve characteristics into a serum reaction mathematical model, a plasma reaction mathematical model and a blood reaction mathematical model so as to acquireC-reactive protein concentration values cS and cP output of the serum sample and the plasma sample, and then acquiring an HCT detection value Hb of the blood sample; and performing HCT correction on the C-reactive protein concentration value cb' output by the blood reaction mathematical model, and taking the C-reactive protein concentration value cb' obtained after HCT correction as output. The C-reactive protein concentration values of different samples are obtained by obtaining the characteristics of the specific protein reaction curves of the different homologous samples, so that the different homologous samples keep consistent C-reactive protein detection results on the same detection equipment.

Description

Technical field [0001] The present invention relates to the field of body fluid detection, and more particularly to a particular protein reaction detection method and apparatus. Background technique [0002] C-reactive protein (CRP) is an acute inflammatory timing phase reaction protein synthesized by the liver. The concentration of the CRP in normal human blood is very low, and the synthesis of the body is sudden, tissue trauma and various inflammatory stimuli, and the blood increases from the hepatocytes, and can be detected 12-18 hours after infection. Horizontal CRP. The 12-14 days after the infection, the high CRP can be reduced to the baseline level. Therefore, over the years - it is one of the indicators of inflammatory diseases, and the increase amplitude is related to the degree of infection. CRP is one of the important markers for diagnosing bacterial infections, which has been widely used in clinical. CRP is also a clinical indicator for assessing the incidence, recurr...

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Problems solved by technology

[0007] At present, there is no CRP standard material of human serum matrix in China. Due to the high price of international standard materials, long transportation cycle, and unguaranteed transportation conditions, it cannot be used for the correctness verification of external quality evaluation agencies, and it cannot meet the increasing demand in my country. The needs of national enterprises for CRP high-level reference materials

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