50 results about "Chain gene" patented technology

A transgenic silkworm having transferred therein a gene which encodes spider thread protein having desired properties of high strength and high elasticity while leaving the silkworm fibroin H chain gene intact, by means of utilizing a transposon function, is used to produce in the transgenic silkworm a spider thread protein having the desired properties without lowering the strength or elasticity of silk thread produced by the transgenic silkworm, thereby providing hybrid silk of spider and silk threads having the desired properties.

The present invention provides a human antibody against blood coagulation factor VIII (hereinafter also referred to as “FVIII”) and an antibody fragment that binds to human FVIII and specifically inhibits the coagulation activity of human FVIII. ScFv display phage libraries, prepared by using scFv DNAs constructed by random combinations of immunoglobulin VH chain genes and VL chain genes from lymphocytes from hemophilia A patients, is reacted with FVIII immobilized to a solid phase via anti-FVIII monoclonal antibody, and scFv clones capable of binding to FVIII are cloned to reveal VH and VL chains of FVIII-specific antibody.

The present invention provides a genetic engineering material for insects that enables a target protein to be purified easily, without requiring the use of recombinant baculovirus, while simultaneously providing a process for producing exogenous protein using that genetic engineering material. A gene recombinant silkworm is obtained by inserting an exogenous protein gene such as a cytokine gene coupled to a promoter that functions in silk glands into a silkworm chromosome. An exogenous protein such as a cytokine is then extracted and purified from the silk glands or cocoon of that silkworm or its offspring. A large amount of exogenous protein can be produced within silk gland cells, outside silk gland cells or in silk thread or a cocoon by inserting an expression gene cassette, in which the DNA sequence of the 3′ terminal portion and the DNA sequence of the 5′ terminal portion of fibroin H chain gene are fused to the exogenous protein gene, into silk gland cells and so forth.

The invention relates to a recombinant immune cytokine targeting PD-L1 and IL-2 receptors and an application of the recombinant immune cytokine. By means of a whole-genome synthesis technology, an Fcheavy-chain gene of an IgG1 subclass antibody of a PD-L1 monoclonal antibody is fused with a mutated IL-2 gene through linker, and the novel recombinant immune cytokine is obtained. The recombinant immune cytokine has better tumor-inhibiting activity, especially for tumor types with PD-L1 weak expression, and achieves effects of activating in-vivo lymphocytes and promoting cytokine production.

The invention discloses an anti-PD-L1 monoclonal antibody. A mouse is immunized with a PD-L1 protein, the spleen of the mouse is separated after successful immunization is determined, total RNA is extracted, reverse transcription is carried out to obtain cDNA, PCR amplification is performed to obtain antibody light-chain and heavy-chain genes, a single-chain antibody phage library is constructed,and screening is performed to obtain the target antibody. The anti-PD-L1 monoclonal antibody screened from the phage antibody library has good affinity to PD-L1, and can inhibit the combination of PD-L1 and PD-1. The anti-PD-L1 monoclonal antibody is used as an immune checkpoint inhibitor after being further humanized, and can be used for treating cancers and infectious diseases.

The present invention provides, in certain aspects, a method of identifying a subject as having an increased risk of developing multiple sclerosis, comprising detecting in the subject the presence of a nucleotide variant in the interleukin 7 receptor alpha chain gene, whereby the presence of said variant identifies the subject as having an increased risk of developing multiple sclerosis.

The present invention provides a polynucleotide comprising all or a part of the nucleotide sequence identified as SEQ ID NO:1 that regulates transcription of the human high-affinity IgE receptor (FcεRl) β-chain gene, and a method for regulating transcription of the FcεRl β-chain gene which comprises the step of promoting binding between a transcription regulatory complex containing MZF-1 and the transcription regulatory region of the FcεRl β-chain gene that contains the nucleotide sequence identified as SEQ ID NO: 1. In addition, the present invention provides a screening method and a kit therefor that utilizes the method for regulating transcription of the FcεRl β-chain gene having the step of promoting binding between a transcription regulatory complex containing MZF-1 and the transcription regulatory region of the FcεRl β-chain gene that contains the nucleotide sequence identified as SEQ ID NO:1, thereby enabling development of a novel agent for the prevention and treatment of allergic diseases.

A human artificial chromosome capable of being efficiently transferred to offspring (next generation) and a method of use thereof. That is, a human artificial chromosome in which a region of about 3.5 Mb to about 1 Mb containing the antibody lambda light chain gene derived from human chromosome 22 is linked to a chromosomal segment derived from another human chromosome, which can be transmitted through the reproductive system of non-human animals to offspring; a non-human animal having the artificial human chromosome and its offspring; a method for constructing the non-human animal; a method for constructing a human antibody using the non-human animal or its offspring; and a production human having the above-mentioned human artificial chromosome antibody mice.

The invention discloses a silk protein peptide fragment for identifying a novel natural color silkworm. A gene sequence of the silk protein peptide fragment comprises domestic silkworm silk H chain amorphous region genes, recombination genes of an H chain crystalline region and an H chain amorphous region, tussah H chain genes and natural silkworm silk H chain genes. The invention further discloses a preparation method of the silk protein peptide fragment. The preparation method comprises the following steps of cloning of the silkworm silk amorphous region genes of the domestic silkworm variety and the establishment and expression of an expression vector; cloning of the recombination genes of the silkworm silk crystalline region and the silkworm silk amorphous region of the domestic silkworm variety and the establishment and expression of an expression vector; and cloning of the silkworm silk genes of the natural color cocoon variety and the establishment and expression of an expression vector. The method is simple and easy to implement, and ensures that the sequence is accurate and errorless, and the gene mutation or the gene deletion of each established expression vector is avoided, so that the triple-coded mispairing or the exceptional amino acid errors of expression products cannot be generated, and thus an obtained product can be further prepared into a silkworm silk antibody with high precision. At the present, the report related to the preparation of the peptide fragment from the white domestic silkworm silk variety (silk protein amorphous regions and crystalline regions/ amorphous region units) and the yellow tussah silk variety or green natural silkworm silk variety is not provided.

The invention provides a preparation method for comprehensively amplifying a humanTCR beta chain library by adopting a small amount of degenerate primers, and relates to the technical field of amplification preparation of an immune repertoire sequencing library. Lymphocyte lysis and total RNA (Ribonucleic Acid) extraction: step one, preprocessing a sample, and then extracting RNA; step 2, reverse transcription: carrying out reverse transcription on the obtained total RNA of the lymphocyte to obtain cDNA (complementary deoxyribonucleic acid); step 3, multiplex PCR: carrying out a first round of PCR and a second round of PCR on the cDNA, and carrying out specific amplification on the TCR beta chain gene of the cDNA; 4, a sequencing linker sequence and index are added, the constructed library is short but contains a complete CDR3 segment, higher flux and lower price can be obtained, the adding amount of each specific primer and an amplification system are determined after a large number of experiments are optimized, the number of primer dimers can be greatly reduced, and the high-quality library is obtained.

The invention provides a chick IFN-gamma receptor alpha chain (IFNGR1) gene which comprises a nucleotide sequence shown in SEQ ID No.1 or a nucleotide sequence which is obtained after changing, adding and/or deleting one or a plurality of nucleotides in the prior nucleotide sequence and has the same functions. The invention also provides a protein chick IFNGR1 coded by the gene, and proteins have functions of ligand synthesis and transmission of IFN-gamma stimulus cue. Chick IFNGR1 extracellular fused proteins have antivirus activity and are a potential antivirus biological agent.

When genes encoding three kinds of proteins constituting fibrinogen, an α chain (or variant of α chain), a β chain and a γ chain (or variant of γ chain) are incorporated into an animal cell, a constitutional ratio of respective genes is such that a γ chain (and/or variant of γ chain) gene is an equal amount to a 1000-fold amount relative to an α chain (and/or variant of α chain) gene and a β chain gene and, further, by using a baculovirus P35 gene, a recombinant fibrinogen highly producing cell is prepared.

The invention provides a preparation method and applications of a shrimp clathrin light chain gene and a targeted dsRNA thereof, relating to a clathrin light chain gene. The clathrin light chain gene originates from C. quadricarinatus, and is named as Cq-CLC. The dsRNA targeted gene of the targeted shrimp clathrin light chain gene is Cq-CLC, and is named as Cq-CLC dsRNA. The preparation method comprises the following steps: 1) designing a primer and preparing dsRNA referring to a kit; 2) transfecting C. quadricarinatus Hpt cells by the dsRNA obtained in the step 1), and detecting the knock-out efficiency on the targeted gene Cq-CLC; and 3) knocking out the Cq-CLC according to the step 2), infecting WSSV, detecting endocytosis amount of WSSV in the Hpt cells knocked-out by the Cq-CLC, and comparing a control group. The Cq-CLC can be used as an important target spot of developing an anti-WSSV class new drug.

The disclosure pertains to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to target sequences that flank the target genomic region; elongating the 3′ end of the extension probe until the 3′ end of the elongated extension probe is adjacent to the 5′ end of the ligation probe; and ligating the 3′ end of the elongated extension probe with the 5′ end of the ligation probe to produce a ligated probe. The ligated probe can be PCR amplified to produce copies of the target genomic region that can be detected or sequenced. Certain embodiments of the invention also provide methods of producing double stranded probes suitable for capturing and analyzing both strands of a target genomic region in a double stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein.

The invention provides a gene segment for expressing a chimeric antigen receptor with a safety switch. The gene segment for expressing the chimeric antigen receptor with the safety switch comprises anNY-ESO-1 single-chain gene segment and a modified epidermal growth factor receptor gene segment which are connected in sequence. The modified epidermal growth factor receptor gene segment comprises an extracellular structural domain III base sequence, an extracellular structural domain IV base sequence and a transmembrane structural domain base sequence of an epidermal growth factor receptor. Onthe one hand, the antigen receptor has improved targeting ability to kill malignant tumor cells. On the other hand, after the CAR-NK cells generate corresponding side effects in the treatment process,the CAR-NK cells can be rapidly removed through the ADCC effect of the NK cells.