112 results about "Chromosome fragment" patented technology

The invention discloses a gossypium barbadense chromosome segment capable of remarkably improving verticillium wilt resistance of gossypium hirsutum and molecular markers of the gossypium barbadense chromosome segment. The gossypium barbadense chromosome segment D4-1 is derived from gossypium barbadense H7124, is located in NO. D4 chromosome of cotton chromosome sets, and is identified through 11 pairs of SSR (simple sequence repeat) markers (including NAU3392, NAU6992, NAU6993, NAU3791, cgr6409, JESPR220, NAU5294, NAU7290, ZHX1, ZHX6 and ZHX29). DNA of the gossypium barbadense H7124 is taken as a template, the 11 pairs of SSR markers are adopted for amplification simultaneously, and a chromosome segment capable of simultaneously containing 11 SSR marker specific bands is the chromosome segment D4-1 of the gossypium barbadense H7124. A disease index of a gossypium hirsutum introgression line containing the chromosome segment is obviously lower than that of a gossypium hirsutum receptor control variety Sumian 8. The gossypium barbadense chromosome segment and the molecular markers thereof are applied to the verticillium wilt resistant molecular breeding of cotton, the verticillium wilt resistance of the cotton can be greatly improved, and the breeding efficiency of the cotton is improved.

The present invention provides an improved and simplified plant chromosome fluorescence in-situ hybridization method, wherein the method does not include a long-time dewatering process of a specimen slide before hybridization and multiple rinsing processes of the specimen slide after hybridization, such that the method is the technology for carrying out rapid in-situ hybridization on the intranuclear chromosome in a biological cell sample. The method mainly comprises: 1) carrying out variable temperature germination, 8-hydroxy quinoline pretreatment, and fixation of the strong cell division tissue in the plant root tip meristem region with a Carnoy fixation liquid to obtain a metakinesis phase specimen with characteristics of clear image and good chromosome dispersion; 2) after mixing fluorescent probes having different labels, carrying out one time denaturation, and carrying out low temperature storage so as to achieve long term repeated use; 3) carrying out denaturation on the sample slide with a NaOH alcohol solution; and 4) during a hybridization reaction process, simplifying the multi-step dewatering, rinsing and other operation steps so as to substantially simplify the whole complex hybridization process. According to the present invention, the method is particularly suitable for the rapid large-scale detection and analysis of the exogenous chromosomes fragment and the chromosome fragment in the plant distant hybridization and close hybridization species, and the morphology and structure authenticity of the specimen chromosome is well maintained.

The invention discloses a drought-resistant related SSR sequence derived from abnormal cotton and application thereof. The invention provides application of a specific DNA fragment or related biomaterials thereof in cultivation of drought-resistant cotton varieties or strains or improvement of the drought resistance of cotton. The specific DNA fragment is a fragment located on chromosome 5 of abnormal cotton and at least containing a sequence from an SSR molecular marker JAAS6365 (SEQ ID No. 1) to an SSR molecular marker JAAS5604 (SEQ ID No. 2). The related biomaterials are a vector, an expression cassette, recombinant bacteria or transgenic cell line containing the specific DNA fragment. The chromosome fragment provided by the invention has important application value in drought-resistantcotton breeding, and at the same time the SSR molecular marker developed by the invention provides a molecular basis for cultivation of drought-resistant cotton varieties that can be applied to production.