Method for separating two tightly linked rice sterile genes

Abstract

The invention relates to a method for separating two tightly linked rice sterile genes, belonging to the field of rice molecular genetics. The method comprises the following steps of: hybridizing a chromosome segment replacement line AIS34 serving as a female parent and Asominori serving as a male parent, from the F1 generation, hybridizing by selecting semisterile plants to obtain a BC1F1 group, performing continuous backcross by selecting the semisterile plants through marker-assisted selection with combination of phenotype identification in the backcross group, performing backcross till the BC4F1 generation, and then performing selfing to obtain a separated group, and respectively positioning a pollen sterile gene and a spikelet sterile gene which are tightly linked with each other. The result shows that through the adoption of the method, the pollen sterile gene is finely positioned in a 33kb area, and meanwhile, an oogamete sterile gene which is tightly linked is finely positioned in a 90kb area.

Description

technical field [0001] The invention belongs to the field of rice molecular genetics, and relates to a method for isolating two closely linked rice sterility genes. Background technique [0002] Kubo et al. (1999, 2002) used molecular marker-assisted selection technology to construct a set of inter-subspecies genome-wide chromosome segment replacement line populations with the japonica rice variety Asominori as the background and the indica rice variety IR24 as the donor. After years of research, we found that one of the substitution lines AIS34 was crossed with Asominori, F 1 exhibit semi-sterile properties. According to the original map published by Kubo et al. (1999, 2002), AIS34 has a small part of heterozygous genotype fragments on chromosomes 4, 6, and 8, and a homozygous genotype fragment of IR24 chromosome with partial replacement on chromosome 5, and the rest are homozygous. It is the background genotype of Asominori. During many years of planting, we found that ...

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Problems solved by technology

2. The trait of indica-japonica hybrid sterility is a quantitative trait, which is controlled by multiple genes at the same time, and it is very difficult to study

3. Indica-japonica hybrid F 1 The fertility of the hybrid is unstable and is easily affected by the external environment. For example, the external low temperature can easily lead to hybrid F 1 lower pollen fertility

4. Finally, the indica-japonica hybrid F 1 Although the mode of sterility is expressed as the interaction of alleles, there are often many different types of fertility genes closely linked near the same fertility site, and there are genes that control pollen fertility on a certain chromosome segment at the same time. The site of fertility with the female gamete, which brings great difficulties to the study of the mode of action of the male and female gametes

At present, there is no report on the isolation and cloning of closely linked genes of different fertility loci

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