15 results about "Female gametes" patented technology

The invention discloses an application of an AP2-F2 protein polyclonal antibody as a cryptosporidium female gamete positioning tag antibody. The AP2-F2 protein of cryptosporidium parvum is subjected to truncated prokaryotic expression, protein is purified to immunize an ICR mouse, a polyclonal antibody is prepared, ELISA and WB detection of antibody titer are carried out to prove that the polyclonal antibody is effective, then indirect immunofluorescence detection of the antibody is carried out to determine that the polyclonal antibody is located on the female gametes of cryptosporidium parvum, and the polyclonal antibody can be used as a label to locate the female gametes of cryptosporidium parvum. The method plays an important role in localization research of unknown protein of cryptosporidium.

The invention discloses a method for cultivating kelp seedlings through diploid female gametophyte parthenogenesis, and belongs to the field of aquatic product engineering genetic breeding. And hybridizing the female and male single cell cloning lines of the induced protonema to obtain the tetraploid sporophyte. And the tetraploid sporophyte is mature and releases zoospores, diploid gametophytes are germinated and formed, and then male and female separation is performed to obtain diploid female gametophyte clone lines in batches. Under the culture condition provided by the invention, parthenogenesis of a diploid female gametophyte clone line is utilized for seedling culture. The diploid female gametophyte begins to ovulate after 6-7 days, and the ovulation time of the diploid female gametophyte is obviously earlier than that of haploid gametophyte in the same period and under the same condition. The 12-day ovulation rate reaches 86.5%, the 24-day sporophyte conversion rate reaches 98.3%, and most sporophyte forms are normal. In the seedling raising period, parthenogenesis seedling raising of the diploid female gametophyte is completed. The diploid female gametophyte can be used as a brand-new kelp germplasm material to be applied to kelp seedling cultivation.

The invention discloses a method for carrying out nine-fold breeding by utilizing female gametes, and relates to the technical field of biological breeding. Comprising the following steps: step 1, pollinating hexaploid persimmon pollen to a'rattan royal house 'containing 2n eggs; 2, collecting young fruits which are born for 65-70 days after pollination, stripping seeds, putting the seeds into a sterilized triangular flask, treating the seeds for 30 seconds in an ultra-clean workbench by using 70% ethanol, pouring out the seeds, adding a 1% NaClO solution, soaking the seeds for 10 minutes, and shaking the seeds once every 3-4 minutes. According to the method for carrying out nine-fold breeding by utilizing female gametes, in persimmon breeding, compared with a 2n pollen path, the 2n egg path is higher in feasibility, 2n eggs and complete male germplasm with good pollen traits are hybridized, pollination populations are expanded, and polyploidy seedless materials can be obtained.

The invention provides a specific molecular marker for sex identification of male and female gametophytes of gracilaria and application of the specific molecular marker. The specific molecular marker of the female gametophytes of gracilaria is GvF-06, and the nucleotide sequence of the specific molecular marker is shown as SEQ ID NO.1; the specific molecular marker of the gracilaria male gametophyte is GvM-03, and the nucleotide sequence of the specific molecular marker is as shown in SEQ ID NO. 2. By utilizing the specific molecular marker, the sex of male and female gametophytes of gracilaria can be quickly and accurately identified, an important molecular tool is provided for genetic breeding work of gracilaria, and the specific molecular marker has important economic benefits and is suitable for popularization and application.

Methods and compositions used to prevent or decrease reduction, or reduction and recombination, during meiosis in female sporocyte, female gametophytes, or female gametes are described herein. The compositions and methods may include silencing elements and / or use gene-editing technology to decrease or inhibit Nrf4 expression levels or activity. Further provided are compositions and methods for the production of a plant that has ovules comprising non-reduced, or non-reduced and non-recombined, gametes, and / or seeds that contain clonal maternal embryos.

The invention discloses a genetically engineered kelp-based oral mucosal immune activation type HPV (human papilloma virus) vaccine as well as a preparation method and application thereof, and belongs to the technical field of manufacturing of genetically engineered vaccines. A kelp female gametophyte is modified by a genetic engineering method, parthenogenesis of the kelp female gametophyte is induced, a kelp plant stably expressing HPV L1 protein is obtained, and the oral mucosal immune activated HPV vaccine is prepared. According to the HPV vaccine provided by the invention, natural polysaccharide of kelp is used as an adjuvant and is absorbed through intestinal mucosa, so that the adverse reaction caused by traditional vaccine injection can be reduced while the mucosa immune reaction can be effectively activated. By utilizing the characteristic of rapid growth of the kelp and the advantage that the prepared vaccine does not need cold chain transportation, low-cost production and large-range coverage of the HPV vaccine can be realized, and global prevention and eradication of cervical cancer are expected to be promoted.

This patent discloses a breeding method for selecting near-isogenic wheat lines using the isoarm chromosome i4JL of *Thinopyrum besarmentosum*. The i4JL chromosome has a unique fluorescent band, making it easily distinguishable from wheat chromosomes. In a wheat background, it is primarily transmitted through female gametes, with extremely low transmission rates in male gametes. Furthermore, it contains two doses of the blue grain gene. Therefore, the self-crossing progeny of the wheat-*Thinopyrum besarmentosum* i4JL monosomatic addition line MAi4JL always exhibits easily separable blue and non-blue grains. By crossing MAi4JL with any non-blue grain wheat and selecting blue grain seeds from the hybrid progeny for five consecutive generations, near-isogenic wheat lines with different backgrounds and phenotypes (blue and non-blue grains) can be obtained, providing a new method for selecting near-isogenic wheat lines. Since i4JL significantly reduces plant height in different wheat backgrounds and significantly affects gene expression in wheat stems and grains, it has significant application potential in wheat genetic breeding research and applications.

The invention discloses a method for creating poplar triploid, and belongs to the technical field of poplar polyploid induction. The creation method comprises the following steps: performing cross pollination on female flower buds of populus japonica by using pollen of a male parent of a poplar to obtain a female inflorescence, treating the female inflorescence at 39-41 DEG C for 2-4 hours, managing the inflorescence, harvesting seeds, sowing and raising seedlings, and identifying seedlings to obtain the populus japonica triploid plant. According to the method, on the basis of systematically analyzing the development process of the female gametes of the populus japonica, a high-temperature induced female gamete chromosome doubling technology is innovatively adopted, a populus japonica female gamete chromosome doubling induction system is successfully constructed, the triploid induction rate is increased by 63.95%, and a solid foundation is laid for populus japonica triploid germplasm creation.

The utility model discloses a mango ultraviolet mutation breeding device, which relates to the technical field of mango planting and comprises a support frame, a light shield and an ultraviolet lamp. The supporting frame supports the light shield from the inner side or the outer side. And the light shield is made of a non-transparent material and is used for covering the mango trees. The ultraviolet lamp is installed on the inner side of the light shield and used for irradiating ultraviolet light to male and female gametes of mangoes. Compared with the prior art, the mango ultraviolet mutation breeding device disclosed by the utility model is used for mutagenizing male and female gametes of mangoes at the same time under the condition of normal growth of mango trees, and is simple in structure, convenient to operate and good in universality.

The invention relates to a rice fertility related gene mutant and application thereof. The rice fertility related gene mutant is characterized in that a basic group T is inserted between 20th to 21st nucleotides at the beginning of ATG of an OsMADS89 wild type gene, or a basic group A is inserted between 19th to 20th nucleotides at the beginning of the ATG of the OsMADS89 wild type gene. The nucleotide sequences of the OsMADS89 mutant gene are respectively as shown in SEQ ID NO. 1 and SEQ ID NO. 2. The amino acid sequences of the OsMADS89 mutant protein are respectively as shown in SEQ ID NO. 3 and SEQ ID NO. 4. According to the invention, a CRISPR / Cas9 gene editing technology is utilized to carry out fixed-point editing on the OsMADS89, and a material which does not influence the vegetative growth of rice, is fertile in pollen and only has abnormal development of female gametes can be produced by destroying the biological function of the encoding protein of the OsMADS89.

The invention discloses application of OlGS1 and OlEXPB1 genes of oryza longistaminata in a self-incompatible restorer line, and relates to the technical field of rice cultivation and seed production, the key points of the technical scheme are as follows: KI-I2, pollen tube in-vivo germination and other tests prove that the physical properties of male and female gametes of oryza longistaminata are normal, the growth of pollen tubes is hindered during selfing, and the incompatible restorer line is incompatible with oryza longistaminata. The pollen tube grows normally during outcrossing. And screening out a candidate gene OlGS1 causing the self-incompatibility of the oryza longistaminata by combining cytology with RNA-seq. The interaction gene OlEXPB1 is screened from a wild oryza longistaminata anther cDNA library by taking OlGS1 as a bait. Gene knockout verification shows that the growth of a mutant pollen tube on a stigma is hindered, so that infertile rice selfing is caused. A self-incompatible restoring line containing OlGS1 and OlEXPB1 is screened from an RIL population by molecular marker-assisted selection and is matched with hybrid rice backbone parents Y58S, Xinan S and the like which are popularized in a large area in production, and results show that introduction of a self-incompatible gene in two environments does not affect the heterosis of the combination of the self-incompatible gene.

The application belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to application of a new tetraploid rice fertility gene NTFR2. The application uses a gene knockout technology to determine the function of the fertility regulation gene NTFR2 in the new tetraploid rice, and finds that the NTFR2 affects the fertility of the new tetraploid rice. When the function of the NTFR2 gene is lost, the seed setting rate of the mutant is reduced, and the development of the female gametophyte sac exhibits abnormality. Meanwhile, the loss of the function of the NTFR2 gene leads to a significant decrease in the fertility of the new tetraploid rice, but has a smaller influence on the male gametes and other agronomic traits, which indicates that the NTFR2 is a new gene mainly affecting the fertility of the tetraploid rice, and has an important application prospect in the fields of creating a new female sterile line and further promoting the utilization of hybrid vigor of the tetraploid rice.

The invention discloses an environment-friendly gene editing system capable of automatically realizing no transgenic element and application of the environment-friendly gene editing system, and belongs to the technical field of gene engineering. The gene editing system is a gene editing carrier containing a transgenic male gamete poisoning element and a transgenic female gamete poisoning element, wherein the transgenic male gamete poisoning element comprises an AtDUO1 promoter and a modified Barnase gene; and the transgenic female gamete poisoning element comprises an AtDD22 promoter and a modified Barnase gene. When the gene editing system is transfected into a plant, transgenic male gametes and transgenic female gametes can be killed, so that non-transgenic male gametes and non-transgenic female gametes survive and participate in fertilization, and non-transgenic plants are obtained. The gene editing system provided by the invention automatically selects non-transgenic gametes, does not have the problems of pollen escape and environmental pollution caused by exogenous genes, and is an environment-friendly gene editing system.

The present invention discloses a method for cultivating kelp seedlings using parthenogenesis of diploid female gametophytes, belonging to the field of genetic breeding in aquatic engineering. Female and male single-cell clones of induced conchoderma are hybridized to obtain tetraploid sporophytes. The tetraploid sporophytes mature and release zoospores, which germinate to form diploid gametophytes. Male and female gametophytes are then separated and batches of diploid female gametophyte clones are obtained. Under the culture conditions provided by the present invention, seedlings are cultivated using parthenogenesis of the diploid female gametophyte clones. These diploid female gametophytes begin to ovulate within 6 to 7 days, significantly earlier than the ovulation time of haploid gametophytes under the same conditions during the same period. The ovulation rate reaches 86.5% on day 12, and the sporophyte conversion rate reaches 98.3% on day 24. Most of the sporophytes have normal morphology. During the seedling cultivation period, the diploid female gametophytes complete parthenogenesis. The diploid female gametophytes can be used as a new kelp germplasm material for kelp seedling cultivation.

The invention discloses a breeding method for breeding a wheat near-isogenic line by utilizing an elytrigia besa equal-arm chromosome i4JL, the i4JL has specific fluorescent stripes, is easy to distinguish from wheat chromosomes, is mainly transferred through female gametes in a wheat background, is extremely low in male gamete transfer rate, contains two doses of blue-grain genes, and can be used for breeding wheat near-isogenic lines. Therefore, the selfing progeny of the wheat-elytrigia besa i4JL monomer addition line MAi4JL always shows separation of blue grains and non-blue grains which are easy to sort, the MAi4JL is hybridized with any non-blue grain wheat, and after blue grain seeds are selected from the hybrid progeny and continuously planted for five generations, wheat blue grain and non-blue grain near-isogenic lines with different backgrounds and phenotypes are obtained. A new method is provided for the breeding of the near-isogenic line of wheat; due to the fact that i4JL obviously reduces the plant height in different wheat backgrounds and obviously affects gene expression in wheat stalks and grains, the i4JL has important application potential in wheat genetic breeding research and application.