5 results about "Female gametes" patented technology

The invention discloses a method for carrying out nine-fold breeding by utilizing female gametes, and relates to the technical field of biological breeding. Comprising the following steps: step 1, pollinating hexaploid persimmon pollen to a'rattan royal house 'containing 2n eggs; 2, collecting young fruits which are born for 65-70 days after pollination, stripping seeds, putting the seeds into a sterilized triangular flask, treating the seeds for 30 seconds in an ultra-clean workbench by using 70% ethanol, pouring out the seeds, adding a 1% NaClO solution, soaking the seeds for 10 minutes, and shaking the seeds once every 3-4 minutes. According to the method for carrying out nine-fold breeding by utilizing female gametes, in persimmon breeding, compared with a 2n pollen path, the 2n egg path is higher in feasibility, 2n eggs and complete male germplasm with good pollen traits are hybridized, pollination populations are expanded, and polyploidy seedless materials can be obtained.

The invention provides a specific molecular marker for sex identification of male and female gametophytes of gracilaria and application of the specific molecular marker. The specific molecular marker of the female gametophytes of gracilaria is GvF-06, and the nucleotide sequence of the specific molecular marker is shown as SEQ ID NO.1; the specific molecular marker of the gracilaria male gametophyte is GvM-03, and the nucleotide sequence of the specific molecular marker is as shown in SEQ ID NO. 2. By utilizing the specific molecular marker, the sex of male and female gametophytes of gracilaria can be quickly and accurately identified, an important molecular tool is provided for genetic breeding work of gracilaria, and the specific molecular marker has important economic benefits and is suitable for popularization and application.

This patent discloses a breeding method for selecting near-isogenic wheat lines using the isoarm chromosome i4JL of *Thinopyrum besarmentosum*. The i4JL chromosome has a unique fluorescent band, making it easily distinguishable from wheat chromosomes. In a wheat background, it is primarily transmitted through female gametes, with extremely low transmission rates in male gametes. Furthermore, it contains two doses of the blue grain gene. Therefore, the self-crossing progeny of the wheat-*Thinopyrum besarmentosum* i4JL monosomatic addition line MAi4JL always exhibits easily separable blue and non-blue grains. By crossing MAi4JL with any non-blue grain wheat and selecting blue grain seeds from the hybrid progeny for five consecutive generations, near-isogenic wheat lines with different backgrounds and phenotypes (blue and non-blue grains) can be obtained, providing a new method for selecting near-isogenic wheat lines. Since i4JL significantly reduces plant height in different wheat backgrounds and significantly affects gene expression in wheat stems and grains, it has significant application potential in wheat genetic breeding research and applications.

The invention relates to a rice fertility related gene mutant and application thereof. The rice fertility related gene mutant is characterized in that a basic group T is inserted between 20th to 21st nucleotides at the beginning of ATG of an OsMADS89 wild type gene, or a basic group A is inserted between 19th to 20th nucleotides at the beginning of the ATG of the OsMADS89 wild type gene. The nucleotide sequences of the OsMADS89 mutant gene are respectively as shown in SEQ ID NO. 1 and SEQ ID NO. 2. The amino acid sequences of the OsMADS89 mutant protein are respectively as shown in SEQ ID NO. 3 and SEQ ID NO. 4. According to the invention, a CRISPR / Cas9 gene editing technology is utilized to carry out fixed-point editing on the OsMADS89, and a material which does not influence the vegetative growth of rice, is fertile in pollen and only has abnormal development of female gametes can be produced by destroying the biological function of the encoding protein of the OsMADS89.

The application belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to application of a new tetraploid rice fertility gene NTFR2. The application uses a gene knockout technology to determine the function of the fertility regulation gene NTFR2 in the new tetraploid rice, and finds that the NTFR2 affects the fertility of the new tetraploid rice. When the function of the NTFR2 gene is lost, the seed setting rate of the mutant is reduced, and the development of the female gametophyte sac exhibits abnormality. Meanwhile, the loss of the function of the NTFR2 gene leads to a significant decrease in the fertility of the new tetraploid rice, but has a smaller influence on the male gametes and other agronomic traits, which indicates that the NTFR2 is a new gene mainly affecting the fertility of the tetraploid rice, and has an important application prospect in the fields of creating a new female sterile line and further promoting the utilization of hybrid vigor of the tetraploid rice.