2 results about "Female gametes" patented technology

This patent discloses a breeding method for selecting near-isogenic wheat lines using the isoarm chromosome i4JL of *Thinopyrum besarmentosum*. The i4JL chromosome has a unique fluorescent band, making it easily distinguishable from wheat chromosomes. In a wheat background, it is primarily transmitted through female gametes, with extremely low transmission rates in male gametes. Furthermore, it contains two doses of the blue grain gene. Therefore, the self-crossing progeny of the wheat-*Thinopyrum besarmentosum* i4JL monosomatic addition line MAi4JL always exhibits easily separable blue and non-blue grains. By crossing MAi4JL with any non-blue grain wheat and selecting blue grain seeds from the hybrid progeny for five consecutive generations, near-isogenic wheat lines with different backgrounds and phenotypes (blue and non-blue grains) can be obtained, providing a new method for selecting near-isogenic wheat lines. Since i4JL significantly reduces plant height in different wheat backgrounds and significantly affects gene expression in wheat stems and grains, it has significant application potential in wheat genetic breeding research and applications.

The application belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to application of a new tetraploid rice fertility gene NTFR2. The application uses a gene knockout technology to determine the function of the fertility regulation gene NTFR2 in the new tetraploid rice, and finds that the NTFR2 affects the fertility of the new tetraploid rice. When the function of the NTFR2 gene is lost, the seed setting rate of the mutant is reduced, and the development of the female gametophyte sac exhibits abnormality. Meanwhile, the loss of the function of the NTFR2 gene leads to a significant decrease in the fertility of the new tetraploid rice, but has a smaller influence on the male gametes and other agronomic traits, which indicates that the NTFR2 is a new gene mainly affecting the fertility of the tetraploid rice, and has an important application prospect in the fields of creating a new female sterile line and further promoting the utilization of hybrid vigor of the tetraploid rice.