Novel method for breeding kelp seedlings with gametophyte cloning method

A technology of gametophytes and new methods, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of insufficient high-quality macroalgae seedlings and cannot meet the needs of breeding, and achieve low cultivation costs, protection of germplasm resources, The effect of improving quality and yield

Inactive Publication Date: 2012-06-13
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a new method for breeding macroalgae seedlings by gametophyte cloning method, so as to solve the problem that high-quality macroalgae seedlings are insufficient and cannot meet the needs of breeding

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  • Novel method for breeding kelp seedlings with gametophyte cloning method
  • Novel method for breeding kelp seedlings with gametophyte cloning method
  • Novel method for breeding kelp seedlings with gametophyte cloning method

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Embodiment 1

[0027] The experiment was conducted from June 2011 to November 2011. The species of macroalgae is Macrocystis Pyrifera, and the male and female gametophytes were cloned and reproduced in June.

[0028] (1) Cloning and propagation When the zoospores of Macroalgae develop to the gametophyte stage, under an inverted microscope (the microscope is Nikon Ti-U, the magnification is 100 times), a female cell and a male cell are separated by capillary separation technology. Separate the male and female gametophytes, put the separated cells into 100ml of sterile seawater with nutrient salts for culture, keep the temperature and culture temperature at 8°C during operation, and the light intensity at 20-40μEm -2 the s -1 , the nutrient salt is in sterilized seawater: KNO 31.2×10 -4 mol / L,KH 2 PO 4 1.2×10 -5 mol / L. Male and female cells continue to multiply after developing into male and female gametophytes. The environmental conditions during the multiplication process are: the te...

Embodiment 2

[0032] The experiment was carried out from July 2011 to December 2011. The species of macroalgae is Macrocystis Pyrifera, and the male and female gametophytes were cloned and reproduced in July.

[0033] (1) Cloning and propagation When the zoospores of Macroalgae develop to the gametophytic stage, under an inverted microscope (the microscope is Nikon Ti-U, the multiple is 100 times), a female cell and a male cell are separated from each other by capillary separation technology. Separate the male and female gametophytes, put the separated cells into 100ml sterile seawater with nutrient salts and culture them separately, keep the operation temperature and culture temperature at 7°C, and the light intensity at 20-40μEm -2 the s -1 , the nutrient salt is in sterilized seawater: KNO 3 1.5×10 -4 mol / L,KH 2 PO 4 1.5×10 -5 mol / L. Male and female cells continue to multiply after developing into male and female gametophytes. The environmental conditions during the multiplicatio...

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Abstract

The invention provides a novel method for breeding kelp seedlings with a gametophyte cloning method, comprising the following steps of: respectively separating a female cell on a female gametophyte and a male cell on a male gametophyte under a microscope by using a capillary tube separation technology; then respectively cloning the female cell and the male cell by using a cloning vegetative propagation technology to respectively grow the female cell and the male cell into female and male gametophytes; continually breeding the gametophytes; when the gametophytes are bred to a fixed amount, damping a formed cell cluster by a cell stamping machine; mixing the female and male gametophytes and respectively releasing ova and sperms by the female and male gametophytes to spontaneously fertilize;putting fertilized zygotes into nutritive salt to be cultured; and after 10 weeks, obtaining seedlings with the height of about 1.5 cm. According to the invention, a fertilization process and the control of temperature and illumination are realized by manual operation, so that the growth period of offspring seeds is greatly shortened; meanwhile, the female cell and the male cell are separated from the gametophytes by using the capillary tube separation technology to clone and culture, so that the quality and the yield of the seedlings are improved, the cultivation of the kelp can be satisfied; and by utilizing the novel method, the cost for breeding the kelp seedlings is low.

Description

Technical field: [0001] The invention belongs to algae propagation technology, and in particular relates to a new method for breeding giant algae seedlings by gametophyte cloning. Background technique: [0002] Macroalgae are considered to be the macroalgae of choice for seafloor revegetation. Due to their large size and extremely fast growth, macroalgae are especially suitable as "supporting algae forests" for artificial reefs to improve the offshore ecological environment. There is no macroalgae distribution in China. In 1978, the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences adopted low-temperature water transportation. method successfully introduced a macroalgae (Macrocystis pyrifera) larvae and gametophytes from Mexico. Through the research on the biology of giant algae cultivation, combined with the characteristics of my country's sea conditions, the researchers established the artificial cultivation and cultivation technology syst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 叶乃好范晓徐东张晓雯
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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