Novel method for breeding kelp seedlings with gametophyte cloning method
A technology of gametophytes and new methods, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of insufficient high-quality macroalgae seedlings and cannot meet the needs of breeding, and achieve low cultivation costs, protection of germplasm resources, The effect of improving quality and yield
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Embodiment 1
[0027] The experiment was conducted from June 2011 to November 2011. The species of macroalgae is Macrocystis Pyrifera, and the male and female gametophytes were cloned and reproduced in June.
[0028] (1) Cloning and propagation When the zoospores of Macroalgae develop to the gametophyte stage, under an inverted microscope (the microscope is Nikon Ti-U, the magnification is 100 times), a female cell and a male cell are separated by capillary separation technology. Separate the male and female gametophytes, put the separated cells into 100ml of sterile seawater with nutrient salts for culture, keep the temperature and culture temperature at 8°C during operation, and the light intensity at 20-40μEm -2 the s -1 , the nutrient salt is in sterilized seawater: KNO 31.2×10 -4 mol / L,KH 2 PO 4 1.2×10 -5 mol / L. Male and female cells continue to multiply after developing into male and female gametophytes. The environmental conditions during the multiplication process are: the te...
Embodiment 2
[0032] The experiment was carried out from July 2011 to December 2011. The species of macroalgae is Macrocystis Pyrifera, and the male and female gametophytes were cloned and reproduced in July.
[0033] (1) Cloning and propagation When the zoospores of Macroalgae develop to the gametophytic stage, under an inverted microscope (the microscope is Nikon Ti-U, the multiple is 100 times), a female cell and a male cell are separated from each other by capillary separation technology. Separate the male and female gametophytes, put the separated cells into 100ml sterile seawater with nutrient salts and culture them separately, keep the operation temperature and culture temperature at 7°C, and the light intensity at 20-40μEm -2 the s -1 , the nutrient salt is in sterilized seawater: KNO 3 1.5×10 -4 mol / L,KH 2 PO 4 1.5×10 -5 mol / L. Male and female cells continue to multiply after developing into male and female gametophytes. The environmental conditions during the multiplicatio...
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