Cutting propagation method for Mongolian medicine plant Artemisia stelleriana

Abstract

The invention belongs to the technical field of plant cultivation, and particularly relates to a Mongolian medicine plant Artemisia stelleriana cutting propagation method, which comprises the following steps: S1, disinfecting Artemisia stelleriana branches; s2, pruning the disinfected Artemisia stelleriana branches, and performing disinfection again to obtain Artemisia stelleriana cuttage segments; s3, treating the cutting sections of the Artemisia stelleriana by using a rooting agent, and cutting the cutting sections of the Artemisia stelleriana subjected to rooting treatment in a matrix for cutting propagation. The cutting propagation method of the Mongolian medicine plant Artemisia stelleriana shows remarkable beneficial effects in the aspects of ecological environment protection, propagation efficiency improvement, germplasm resource enrichment, stress resistance enhancement, economic benefit improvement and the like, and has important popularization and application values.

Description

Technical Field

[0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for cutting propagation of Mongolian medicinal plant Artemisia annua. Background Art

[0002] The above-ground part of Artemisia frigida, a plant of Artemisia genus, Asteraceae, is warm in nature and pungent in taste. It has the effects of moistening and removing dampness, relieving cough and relieving pain, clearing away heat and promoting diuresis, and reducing inflammation and killing insects. It is a commonly used dry "Xi Ri Wu Su" characteristic Mongolian medicine. At present, the production of Artemisia dahurica is mainly based on wild resource picking, and seed breeding is carried out in some areas. Because Artemisia dahurica belongs to a plant maintained by water bodies, the growth mode of Artemisia dahurica is closely related to the growth environment, and wild picking of Artemisia dahurica is very easy to damage the environment, causing soil erosion, increasing the degree of desertification of grasslands and Artemisia dahurica seed breeding, low emergence rate, scarce germplasm resources, weak stress resistance in the seedling stage, and too slow growth cycle. Therefore, it is necessary to develop a method for cutting propagation of Artemisia dahurica. Summary of the invention

[0003] The invention aims to provide a method for cutting propagation of Mongolian medicinal plant Artemisia annua, so as to overcome the shortcomings of the prior art, adopt cutting propagation, simple operation steps, short seedling raising cycle, large number of seedlings that can be raised per unit area, short seedling acclimatization period after planting, high survival rate, improved breeding efficiency, and easy to promote and apply.

[0004] In order to achieve the above object, the technical solution of the present invention is:

[0005] The present invention provides a method for cutting propagation of Mongolian medicinal plant Artemisia annua, comprising the following steps:

[0006] S1, disinfecting Artemisia annua branches;

[0007] S2, pruning the disinfected Artemisia scoparia branches, and obtaining Artemisia scoparia cuttings after disinfection again;

[0008] S3, treating the Artemisia annua cuttings with a rooting agent, and inserting the rooting Artemisia annua cuttings into a substrate for cutting propagation.

[0009] In some other embodiments, in step S1, the Artemisia annua branches are two to three-year-old Artemisia annua branches with a growth time of more than 60 days; preferably, the Artemisia annua branches are two-year-old Artemisia annua branches with a growth time of more than 60 days.

[0010] In some other embodiments, in step S1, the picking time of the Artemisia annua branches is between 9:00 and 11:00 in the morning, and the length of the branches is 3-5 cm.

[0011] In some other embodiments, the disinfection treatment in step S1 or S2; the concentration of the disinfectant is 0.5-2 g·L-1 , disinfection time is 1-10min;

[0012] Preferably, the concentration of the disinfectant is 1 g·L -1 , the disinfection time is 1min.

[0013] In some other embodiments, the disinfectant used in the disinfection treatment in step S1 or S2 is a disinfectant mixed with harzianum and Bacillus subtilis or carbendazim.

[0014] In some other embodiments, the mixing mass ratio of the harzianum and the bacillus subtilis is 1:(1-5);

[0015] Preferably, the mixing mass ratio of the harzianum and the Bacillus subtilis is 1:3.

[0016] In some other embodiments, in step S3, the rooting agent is one or both of indolebutyric acid and melatonin;

[0017] The concentration of indolebutyric acid is 400-1200 mg·L -1 The concentration of melatonin is 100-300 mg·L -1 .

[0018] In some other embodiments, in step S3, the rooting agent is a mixture of indolebutyric acid and melatonin, and the concentration ratio of indolebutyric acid to melatonin is 800 mg·L -1 :(100-300)mg·L -1 ;

[0019] Preferably, the concentration ratio of indolebutyric acid to melatonin is 800 mg·L -1 :200mg·L -1 .

[0020] In some other embodiments, in step S3, the substrate is nutrient soil, sandy soil, oak powder, perlite, coconut bran and buckwheat husk.

[0021] In some other embodiments, in step S3, the matrix is ​​nutrient soil + sand + oak powder, nutrient soil + perlite + oak powder, nutrient soil + sand + perlite, sand + nutrient soil + coconut bran, sand + nutrient soil + coconut bran, nutrient soil, nutrient soil + sand + buckwheat husk, nutrient soil + sand + buckwheat husk, nutrient soil + buckwheat husk, nutrient soil + buckwheat husk;

[0022] Preferably, the matrix is ​​a mixture of nutrient soil + sand + oak powder in a ratio of 2:2:1, a mixture of nutrient soil + perlite + oak powder in a ratio of 2:2:1, a mixture of nutrient soil + sand + perlite in a ratio of 1:1:1, a mixture of sand + nutrient soil + coconut bran in a ratio of 1:1:1, a mixture of sand + nutrient soil + coconut bran in a ratio of 2:1:1, a mixture of nutrient soil, nutrient soil + sand + buckwheat husk in a ratio of 1:1:1, a mixture of nutrient soil + sand + buckwheat husk in a ratio of 1:1:2, a mixture of nutrient soil + buckwheat husk in a ratio of 1:1, and a mixture of nutrient soil + buckwheat husk in a ratio of 2:1;

[0023] Further preferably, the matrix is ​​a mixture of nutrient soil + perlite + oak powder in a mass ratio of 2:2:1.

[0024] Beneficial effects of the present invention:

[0025] The cutting propagation method of the Mongolian medicinal plant Artemisia dahurica proposed in the present invention has shown significant beneficial effects in protecting the ecological environment, improving propagation efficiency, enriching germplasm resources, enhancing stress resistance and improving economic benefits, and has important promotion and application value. Compared with the traditional wild resource picking and seed breeding methods, it has significant advantages in many aspects, as follows:

[0026] (1) Environmental protection and sustainability: Propagating Artemisia annua by cuttings effectively avoids over-picking of wild resources and significantly reduces the damage to the environment caused by picking activities, such as soil erosion, grassland degradation, and desertification. This is beneficial to the protection and restoration of the ecological environment and realizes the sustainable use of Mongolian medicinal resources.

[0027] (2) Improved propagation efficiency: Compared with seed propagation, cutting propagation has a higher survival rate and growth rate. The present invention significantly improves the survival rate and growth quality of Artemisia dahurica cutting seedlings and shortens the growth cycle by optimizing key technical links such as cutting medium, cutting timing, hormone treatment and subsequent management, thereby greatly improving the propagation efficiency and meeting market demand.

[0028] (3) Enriching germplasm resources: Cutting propagation can rapidly expand the population size while maintaining the excellent traits of the mother plant, avoiding the genetic drift and germplasm degradation problems that may occur in seed propagation. The application of this method helps to enrich the germplasm resource pool of Artemisia annua and provide a better quality and more stable source of raw materials for the Mongolian medicine industry.

[0029] (4) Enhance stress resistance: Through scientific management and appropriate physiological control measures, such as hormone treatment and nutrient supply, the stress resistance of Artemisia annua cuttings can be significantly improved, including drought resistance, cold resistance, disease and pest resistance, so that they can maintain a good growth state under different growth environments and improve yield and quality.

[0030] (5) Significant economic benefits: Due to the high efficiency and stability of cutting propagation, this method can not only quickly increase the yield of Artemisia annua, but also reduce production costs, such as reducing seed procurement costs and shortening the growth cycle to reduce land and labor costs, thereby improving the economic benefits of Mongolian medicine production enterprises and promoting local economic development. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0032] Figure 1 is a photo of Artemisia annua cuttings seedlings according to an embodiment of the present invention;

[0033] Figure 2 This is a photo of the Artemisia annua cutting medium in the embodiment of the present invention;

[0034] Figure 3 This is a picture of the fermentation product of Mongolian Oak dead leaves and seeds in the examples of the present invention. DETAILED DESCRIPTION

[0035] All raw materials and reagents used in the present invention are commercially available products.

[0036] The present invention provides a method for cutting propagation of Mongolian medicinal plant Artemisia annua, comprising the following steps:

[0037] S1, disinfecting Artemisia annua branches;

[0038] S2, pruning the disinfected Artemisia scoparia branches, and obtaining Artemisia scoparia cuttings after disinfection again;

[0039] S3, treating the Artemisia annua cuttings with a rooting agent, and inserting the rooting Artemisia annua cuttings into a substrate for cutting propagation.

[0040] The solution of the present invention is further described in detail below in conjunction with specific implementation modes, as follows:

[0041] Example 1

[0042] A method for cutting propagation of Mongolian medicinal plant Artemisia annua, which studies the effects of different seedling ages on the rooting rate and survival rate of cuttings, and the specific operating steps are as follows:

[0043] S1: Pick Artemisia sphaerocephala branches of different seedling ages between 9 and 11 am on a sunny day, and select branches with a growth time of more than 60 days and a high degree of lignification, with a branch length of 3 to 5 cm. Use different disinfectants to treat the picked Artemisia sphaerocephala branches for 10 minutes to prevent the incision from being infected by pathogens, which may cause diseases such as black stems and prevent rooting.

[0044] S2: Prune the Artemisia scoparia branches disinfected in step S1 to remove old branches, old leaves and diseased leaves. After pruning, disinfect again;

[0045] S3: The mother plants of different ages treated in step S2 were cut into a matrix of nutrient soil + perlite + vermiculite (mixed in a mass ratio of 1:1:1). Thirty cuttings were cut into the matrix, and the experiment was repeated 3 times.

[0046] The effects of the types of disinfectants used in steps S1 and S2 on the stress resistance of cuttings were studied as shown in Table 1. As can be seen from Table 1, harzian berry + Bacillus subtilis (mixed mass ratio 1:3, i.e. 0.25g harzian berry + 0.75g Bacillus subtilis in 1L water) is the best soil disinfectant, which increases the stress resistance of cuttings. This is because Trichoderma harzianum has a strong mycelium growth and spread ability, as well as the bactericidal function of parasitic pathogens, effectively competing with pathogens for nutrients and space, thereby reducing the damage of pathogens to Artemisia small white cuttings. The antibiotics, biological enzymes and active substances secreted by it can stimulate the growth and development of plant cells and improve the stress resistance of Artemisia small white cuttings; the induction resistance ability of Trichoderma harzianum can also make Artemisia small white cuttings show stronger resistance when facing adversity. Although Bacillus subtilis can also produce antibiotics and plant growth regulators to inhibit pathogens and promote plant growth, its effect is relatively mild and not as strong as that of Trichoderma harzianum. Bacillus subtilis mainly improves the stress resistance of Artemisia small white cuttings by improving the soil environment and providing nutrients.

[0047] At a mixing ratio of 1:3 between Harzianberry and Bacillus subtilis, the number of Trichoderma harzianum is relatively small, but due to its strong mycelium growth and spread ability, bactericidal function of parasitic pathogens and inducing resistance, it can still play an important role in enhancing the stress resistance of Artemisia small white cuttings. Although Bacillus subtilis is present in large numbers, its effect is relatively mild, mainly by improving the soil environment and providing nutrients. Therefore, at a mixing ratio of 1:3 between Harzianberry and Bacillus subtilis, Trichoderma harzianum may perform better in enhancing the stress resistance of Artemisia small white cuttings.

[0048] Table 1 Effects of different soil disinfectants on the stress resistance of cutting seedlings

[0049]

[0050] The effects of different seedling ages on the rooting rate and survival rate of cuttings are shown in Table 2. It can be seen from Table 2 that the rooting time, rooting rate, total number of roots and root length of two-year-old Artemisia sphaerocephala are significantly different from those of three-year-old Artemisia sphaerocephala. As a perennial herb, the biological characteristics and growth patterns of Artemisia sphaerocephala are important factors affecting the survival rate of cuttings. Generally, two-year-old plants have stronger regeneration ability and adaptability than three-year-old plants. This is mainly attributed to the fact that young plants contain fewer root-inhibiting substances, while nutrients are mainly used for nutritional growth, so the rooting ability and survival rate of their branches are usually higher.

[0051] Table 2 Effects of different seedling ages on rooting rate and survival rate of cuttings

[0052]

[0053] Cuttings from two-year-old Artemisia annua plants are usually more tender, with active cell division and higher physiological metabolism levels. This vitality helps the cuttings quickly adapt to the new environment after cutting and start the rooting process. In contrast, cuttings from three-year-old plants may have weakened cell division ability and reduced physiological metabolism levels due to aging, resulting in reduced rooting ability.

[0054] Cuttings from two-year-old plants usually contain more soluble sugars, amino acids and other nutrients, which are necessary for cell division and growth during the rooting process. As the plant ages, the content of these nutrients may gradually decrease, thus affecting the rooting ability of the cuttings.

[0055] Cuttings from three-year-old plants may contain more root-inhibiting substances, which inhibit the initiation and progress of the rooting process. In contrast, cuttings from young plants contain fewer root-inhibiting substances and are more conducive to the rooting process.

[0056] Young plants (e.g. 2-year-olds) are usually more adaptable to new soil and climate conditions. Figure 1 This helps the cuttings to take root and grow quickly after cutting, thereby increasing the survival rate. Therefore, the two-year-old Artemisia argyi seedlings are selected as the best cutting material.

[0057] Example 2

[0058] Harzianum husk + Bacillus subtilis (mixing mass ratio 1:3, i.e. 0.25g Harzianum husk + 0.75g Bacillus subtilis in 1L water) were used as disinfectants, and two-year-old Artemisia annua seedlings were the best cutting material. The operating procedures of steps S1 and S2 were the same as those in Example 1. The difference from Example 1 was that different substrates were used to treat the Artemisia annua branches in step S3.

[0059] Specifically, in step S3, different matrices were studied to treat Artemisia annua branches, and the matrices used were as follows (each component was a mixed mass ratio): nutrient soil + sand + oak powder (2:2:1), nutrient soil + perlite + oak powder (2:2:1), nutrient soil + sand + perlite (1:1:1), sand + nutrient soil + coconut bran (1:1:1), sand + nutrient soil + coconut bran (2:1:1), nutrient soil, nutrient soil + sand + buckwheat husk (1:1:1), nutrient soil + sand + buckwheat husk (1:1:2), nutrient soil + buckwheat husk (1:1), nutrient soil + buckwheat husk (2:1). Thirty cuttings were planted in each group of matrices, and the experiment was repeated three times. The experimental results are shown in Table 3. It can be seen from Table 3 that nutrient soil + perlite + oak powder (2:2:1) is the best matrix, and oak powder is the fermentation product of crushed Mongolian oak leaves and seeds. Figure 3 Mongolian oak leaves and seeds are rich in organic matter and a variety of mineral elements. After fermentation, these ingredients are broken down into a form that is easier for plants to absorb.

[0060] Organic matter can provide the carbon source and energy required by plants, while improving soil structure and increasing soil water retention and air permeability. Mineral elements are necessary for the normal growth and development of plants, and have a positive effect on promoting the rooting of Artemisia dahurica cuttings. During the fermentation process, certain components in Mongolian oak leaves and seeds may be converted into plant growth regulators, such as gibberellins and cytokinins. These growth regulators can promote the division and growth of plant cells, thereby facilitating the rooting of Artemisia dahurica cuttings.

[0061] Fermentation products contain a variety of enzymes, such as cellulase, protease, etc. These enzymes can decompose organic matter in the soil, release nutrients for plants to absorb and utilize, and promote the division and growth of plant cells.

[0062] Adding fermentation products can improve soil structure and increase soil looseness and air permeability, which is beneficial to the respiration and water absorption of the roots of Artemisia argyi cuttings and promotes rooting.

[0063] Fermentation products can also adjust the pH of the soil. Appropriate soil pH helps plants absorb and utilize nutrients, thereby promoting rooting. These effects together promote the development and growth of the root system of Artemisia argyi cuttings, and improve their survival rate and stress resistance. Figure 2 shown.

[0064] Table 3 Effects of different substrates on rooting rate and survival rate of cuttings

[0065]

[0066] Example 3

[0067] Two-year-old Artemisia scoparia was selected as the best cutting material, and nutrient soil + perlite + oak powder (2:2:1) was selected as the best matrix. The operation process of steps S1 and S2 was the same as that of Example 1. The difference from Example 1 was that in step S3, before cutting, the Artemisia scoparia branches were placed in different rooting agents and dipped at medium speed for 10 seconds. The effects of the types and concentrations of the rooting agents on the Artemisia scoparia branches were investigated.

[0068] The rooting agent and concentration (mg·L) used in step S3 -1 ) are as follows: IBA400, IBA 800, IBA 1200, melatonin 100, melatonin 200, melatonin 300, IBA800+melatonin 100, IBA800+melatonin 200, IBA800+melatonin 300; wherein IBA is indolebutyric acid. The research results are shown in Table 4. It can be seen from Table 4 that IBA800mg / l+melatonin 200mg / l is the best rooting agent combination. IBA and melatonin have complementary effects in promoting plant rooting. IBA mainly promotes rooting by promoting cell division and differentiation, increasing nutrient accumulation and regulating enzyme activity; while melatonin plays a role by scavenging free radicals, anti-oxidation, regulating hormone balance and improving stress resistance. The synergistic effect of the two can more effectively promote the rooting of Artemisia small white cuttings.

[0069] IBA and melatonin have a synergistic effect in regulating plant physiological processes. They can jointly regulate metabolic activities in plants, promote the accumulation and distribution of nutrients, and provide sufficient energy and material basis for rooting. Through the synergistic effect of IBA and melatonin, the rooting quality and speed of Artemisia dahurica cuttings can be significantly improved. This is not only manifested in the increase in the number of roots, but also in the thick root system and vigorous growth.

[0070] Table 4 Effects of different rooting agents on rooting rate and survival rate of cuttings

[0071]

[0072] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for cutting propagation of Mongolian medicinal plant Artemisia annua, characterized in that: The following steps are involved: S1, disinfecting Artemisia annua branches; S2, pruning the disinfected Artemisia scoparia branches, and obtaining Artemisia scoparia cuttings after disinfection again; S3, treating the Artemisia annua cuttings with a rooting agent, and inserting the rooting Artemisia annua cuttings into a substrate for cutting propagation.

2. The method for cutting propagation of Mongolian medicine plant Artemisia annua according to claim 1, wherein: In step S1, the wormwood branches are two to three years old wormwood branches with a growth time of more than 60 days; Preferably, the Artemisia annua branches are two-year-old Artemisia annua branches with a growth period of more than 60 days.

3. The method for the cuttage propagation of Mongolian medicine plant Artemisia annua according to claim 1, wherein: In step S1, the picking time of the Artemisia scoparia branches is between 9:00 and 11:00 in the morning, and the length of the branches is 3-5 cm.

4. The method for the cuttage propagation of Mongolian medicine plant Artemisia annua according to claim 1, wherein: Disinfection in step S1 or S2; the concentration of the disinfectant is 0.5-2g·L -1 , disinfection time is 1-10min; Preferably, the concentration of the disinfectant is 1 g·L -1 , the disinfection time is 1min.

5. The method for the cuttage propagation of Mongolian medicine plant Artemisia annua according to claim 1, wherein: The disinfectant used in the disinfection treatment in step S1 or S2 is a disinfectant mixed with harzianum and Bacillus subtilis or carbendazim.

6. The method for the cuttage propagation of Mongolian medicine plant Artemisia annua according to claim 5, wherein: The mixing mass ratio of the harzianum and the bacillus subtilis is 1:(1-5); Preferably, the mixing mass ratio of the harzianum and the bacillus subtilis is 1:

3.

7. The method for the cuttage propagation of Mongolian medicine plant Artemisia annua according to claim 1, wherein: In step S3, the rooting agent is one or both of indolebutyric acid and melatonin; The concentration of indolebutyric acid is 400-1200 mg·L -1 The concentration of melatonin is 100-300 mg·L -1 .

8. The method for the cuttage propagation of Mongolian medicine plant Artemisia annua according to claim 7, wherein: In step S3, the rooting agent is a mixture of indolebutyric acid and melatonin, and the concentration ratio of indolebutyric acid to melatonin is 800 mg·L -1 :(100-300)mg·L -1 ; Preferably, the concentration ratio of indolebutyric acid to melatonin is 800 mg·L -1 :200mg·L -1 .

9. The method for the cuttage propagation of Mongolian medicine plant Artemisia annua according to claim 1, wherein: In step S3, the matrix is ​​nutrient soil, sandy soil, oak powder, perlite, coconut bran and buckwheat husk.

10. The method for cutting propagation of Mongolian medicine plant Artemisia annua according to claim 9, characterized in that, In step S3, the matrix is ​​nutrient soil + sand + oak powder, nutrient soil + perlite + oak powder, nutrient soil + sand + perlite, sand + nutrient soil + coconut bran, sand + nutrient soil + coconut bran, nutrient soil, nutrient soil + sand + buckwheat husk, nutrient soil + sand + buckwheat husk, nutrient soil + buckwheat husk, nutrient soil + buckwheat husk; Preferably, the matrix is ​​a mixture of nutrient soil + sand + oak powder in a ratio of 2:2:1, a mixture of nutrient soil + perlite + oak powder in a ratio of 2:2:1, a mixture of nutrient soil + sand + perlite in a ratio of 1:1:1, a mixture of sand + nutrient soil + coconut bran in a ratio of 1:1:1, a mixture of sand + nutrient soil + coconut bran in a ratio of 2:1:1, a mixture of nutrient soil, nutrient soil + sand + buckwheat husk in a ratio of 1:1:1, a mixture of nutrient soil + sand + buckwheat husk in a ratio of 1:1:2, a mixture of nutrient soil + buckwheat husk in a ratio of 1:1, and a mixture of nutrient soil + buckwheat husk in a ratio of 2:1; Further preferably, the matrix is ​​a mixture of nutrient soil + perlite + oak powder in a mass ratio of 2:2:1.

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