Method for reducing aberration rate of kelp germplasm parthenogenesis sporophytes

A technology of young sporophyte and female gametophyte, which is applied in the field of reducing the parthenogenetic deformity rate of female germplasm of kelp, and can solve problems such as environmental conditions not meeting the special requirements of parthenogenetic growth, high parthenogenous deformity rate, and missing chromosomes. Achieve good synchronization, shorten ovulation time, and high ovulation rate

Active Publication Date: 2020-02-11
MARINE BIOLOGY INST OF SHANDONG PROVINCE
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Problems solved by technology

However, the deformity rate of parthenogenesis is very high, generally reaching about 80%. The environmental conditions of cultivation do not meet the special requirements of parthenogenetic growth

Method used

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  • Method for reducing aberration rate of kelp germplasm parthenogenesis sporophytes
  • Method for reducing aberration rate of kelp germplasm parthenogenesis sporophytes
  • Method for reducing aberration rate of kelp germplasm parthenogenesis sporophytes

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Embodiment 1

[0032] Embodiment 1: promote kelp germplasm to discharge ovum

[0033] 1) Prepare germplasm material for parthenogenetic development

[0034] The female germplasm filaments of kelp (Saccharina japonica) with dark brown color and no algae pollution were selected from the germplasm bank, and part of the germplasm was poured out for induction materials. Those used to induce germplasm were placed in 500ml sterile sea water.

[0035] 2) Pre-treatment for induction

[0036] The germplasm used for induction was placed in an induction incubator 24 hours before induction, the culture temperature was 15±1°C, the light source was a fluorescent tube, and the light was 1000lx, increasing by 500lx per hour until 6000±200lx.

[0037] 3) Ovulation induction treatment

[0038] The germplasm was crushed with a tissue grinder at 1500 rpm for 30 seconds, and repeated once. Then filter three times with a single-layer 500-mesh sieve to obtain filament fragments with a length of 3-6 cells.

[0...

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Abstract

The invention provides a method for facilitating ovulation of kelp female gametophytes. The method includes the steps: resuscitating kelp germplasm filaments in seawater, crushing kelp germplasms, andfiltering crushed liquid to obtain filament fragments with the lengths of 3-6 cells; performing aerated suspension culture on the filament fragments under the conditions of the illumination intensityof 6000+/-300lx, the illumination cycle of L:D=12h:12h and the temperature of 15+/-1 DEG C to collect eggs; culturing the collected eggs to obtain young kelp sporophytes, culturing the young kelp sporophytes, and performing culture by running water at the stage of young sporophytes of two rows of cells. According to the method, female kelp germplasms can ovulate in 11-13 days, ovulation time is shortened, ovulation rate is high, ovulation synchronization is good, ovulation rate within two days can reach 65-70%, and egg development rate can reach 70-80%. According to the built culture method of young sporophytes, the aberration rate of the young sporophytes can be reduced to 50-60%.

Description

technical field [0001] The invention belongs to the technical field of kelp breeding, and in particular relates to a method for reducing the parthenogenic deformity rate of kelp female germplasm. Background technique [0002] Kelp is a large-scale economic brown alga with heterogeneous generation alternation. The life history of natural growth of kelp includes short-term gametophytes and long-term sporophyte stages. Mature kelp sporophytes release to produce zoospores. After the zoospores attach, they develop into female and male gametophytes, and the gametophytes respectively Sperm eggs are produced, the eggs are fertilized to form zygotes, and the zygotes develop into young sporophytes. But there is also a parthenogenetic pathway in which the female germplasm releases eggs and the eggs split into young sporophytes in kelp. [0003] Parthenogenesis is of great significance for breeding. If it can develop into adults, it can accelerate the homozygous speed of offspring and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00A01H4/00
CPCA01G33/00A01H4/001A01H4/005
Inventor 王翔宇辛美丽吕芳吴海一詹冬梅
Owner MARINE BIOLOGY INST OF SHANDONG PROVINCE
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