30results about How to "Increase ovulation rate" patented technology

The present invention provides a method for enhancing embryo viability comprising administering at least one inhibitor of p53 or a p53-associated pathway to one or more of the following: the embryo, oocytes, sperm, a femme animal or a male animal.

The invention discloses a method for inducing Stichopus Japonicus Selenka to lay eggs in vivo, which is characterized by simple operation, short time, synchronous egg laying, large discharge quantity and good quality of eggs, and capability of greatly improving induction rate, fertility rate and hatching rate; the method is implemented as the following steps: dissolving NGLWY acid amides in ultrapure water to formulate 1 mumol / L mother liquor, then dissolving the mother liquor in 0.5mol / L KCl sea water solution to prepare 1nmol / L inducer; selecting gonad mature parent Stichopus Japonicus Selenka with the weight being at least 200g; injecting the inducer with the ratio of volume and the weight of parent Stichopus Japonicus Selenka being 0.1% ml / g but less than 0.3ml into coelom from the back of the Stichopus Japonicus Selenka to perform in vivo induction; and collecting the eggs.

A slow-releasing oxytocic for artificially inducing the maturation of epinephelus' sex gland and the ovulation or ejaculation by embedding it in body of epinephelus is prepared from luliberin like, 17 alpha-hydroxyprogesterone, cholesterin, cellulose and plant oil through proportional mixing and moulding.

The invention discloses a method for preparing an inhibin genetic engineering regulation antigen, which comprises the following steps: cloning an inhibin encoding gene; making use of the inhibin encoding gene to prepare an inhibin recombinant fusion protein; and making use of the inhibin recombinant fusion protein to prepare the inhibin genetic engineering regulation antigen. The method for preparing the inhibin genetic engineering regulation antigen has the advantages of simplicity, high purity, good immunogenicity, low price and the like; the inhibin genetic engineering regulation antigen prepared by the method does not have toxicity, pathogenicity and drug residue, is harmless to human and animals, has no adverse effect on consumers, can improve the ovulation rate and the litter size, improve utilization ratio of a superovulation embryo, promote the sexual maturation so as to improve the reproduction and the like.

The invention provides a pharmaceutical composition for treating polycystic ovary syndrome (PCOS). The pharmaceutical composition consists of a traditional Chinese medicine composition and chemical drugs, wherein the traditional Chinese medicine composition comprises the medicinal materials such as anemarrhena, fritillary bulb, tortoise plastron, fleece-flower root, lilyturf root, hippocampus andthe like; and the chemical drugs comprise one or more compound molecules of dexamethasone, spirolactone, ethinyloestradiol, estradiol valerate, conjugated estrogen and pharmaceutically acceptable salt. The pharmaceutical composition has the advantages of reducing the level of androgens and androgen receptors of patients suffering from PCOS, improving the level of sex hormone-binding globulin (SHBG), lowering the level of free testosterone (F-TESTO), further raising the level of in-vivo estrogens, improving growth and development of follicles and increasing ovulation rate and pregnancy rate ofthe patients.

The invention provides a traditional Chinese medicine composition for treating kidney-yin deficiency and phlegm-blood stasis polycystic ovary syndrome (PCOS). The traditional Chinese medicine composition comprises the medicinal materials such as anemarrhena, fritillary bulb, tortoise plastron, radix polygoni multiflori preparata, lilyturf root, hippocampus and the like. The medicinal materials are cooperative with each other so as to realize the efficacies of nourishing kidney and clearing away heat and reducing phlegm and removing blood stasis. The traditional Chinese medicine composition has the advantages of reducing the level of androgens and androgen receptors of patients suffering from PCOS, improving the level of sex hormone-binding globulin (SHBG), lowering the level of free testosterone (F-TESTO), further raising the level of in-vivo estrogens, improving growth and development of follicles and increasing ovulation rate and pregnancy rate of the patients.

Methods are provided for enhancing viability comprising administering at least one inhibitor of p53 or a p53-associated pathway to one or more of the following: the embryo, oocytes, sperm, a femme animal or a male animal.

The invention belongs to the technical field of medical information and discloses an information acquisition system for a joint marker for diagnosing PCOS (polycystic ovarian syndrome). The information acquisition system for the joint marker for diagnosing the PCOS comprises an excessive androgen measurement module, a serum extraction module, a central control module, a joint marker testing module, a treatment module, a data storage module and a display module. A detection kit can realize high-sensitivity and efficient detection and has the characteristics of low detection cost and good repeatability. Meanwhile, pharmaceutical composition provided by the treatment module and used for the excessive androgen type PCOS comprises medicinal materials including herba epimedii, fritillaria, fructus psoraleae, antler slices, hippocampus, tortoise plastron and the like, does not contain radix et rhizoma rhei or radix angelicae sinensis, notably reduces the androgen level of a patient, improvesgrowth of ovarian follicle and increases the ovulation rate and the pregnancy rate of the patient in the child-bearing period after being taken for a period of time.

The invention discloses cattle expand breeding method inlivestock raising technology. The feed of the cattle is prepared from, by weight, 200 to 250 parts of silage, 100 to 200 parts of corn flour, 70 to 80 parts of beet pulp, 30 to 50 parts of black bean powder, 10 to 20 parts of corncobs, 1 to 3 parts of chalcopyrite, 1 to 3 parts of chrysanthemum, 1 to 3 parts of antler gum, 1 to 3 parts of herba epimedii, 1 to 2 parts of purple quartz, 1 to 3 parts of teasel root, 1 to 3 parts of Achyranthes bidentata, 1 to 3 parts of rhizoma chuanxiong, 1 to 3 parts of Velvetleaf, 1 to 3 parts of dried tangerine peel, 1 to 3 parts of rehmannia glutinosa, 1 to 3 parts of Cistanche and 1 to 3 parts of Motherwort. The main materials of the method provide nutrients, while auxiliary materials are helpful in ovulation. Herbal medicine additives helps pregnancy and ovulation, and drugs combination is good to liver, kidney, nourishing tone, promote ovulation, multi-ovulation, and no abnormal fetal is produced, and also follicles and embryo development to provide better endocrine environment, improve the ovulation rate, pregnancy probability, thereby increasing the amount of cattle produced and the number of cattle.

The invention provides a method for increasing the ovulation rate of sinonovacula constricta (Lamarck) parent oysters and guaranteeing ovulation synchronism. An inonovacula constricta (Lamarck) parent oyster ovulation device employed comprises a rectangular bottom frame, supporting rods, a shielding film and a light-dimming window, wherein small holes are drilled in the four corners of the bottom frame, the four supporting rods are inserted into the small holes to form a cuboid-shaped frame, the shading film as high as the supporting rods is put to surround the cuboid-shaped frame, and the light-dimming window is paved at the top of the cuboid-shaped frame; the bottom of the bottom frame is covered with a nylon net; an air inlet pipe is laid in the longitudinal direction of the bottom frame; three air stones are connected under the air inlet pipe. When the inonovacula constricta (Lamarck) parent oyster ovulation device is applied for ovulation, the light intensity in the light-dimming window is controlled at 200Lx, and therefore, the ovulation synchronism of the inonovacula constricta (Lamarck) is guaranteed, and meanwhile, the ovulation rate of the sinonovacula constricta (Lamarck) parent oysters is greatly increased and the shortcomings of the prior art are solved; meanwhile, the whole device is very convenient to assemble and disassemble, easy to clean and low in cost, and cannot be corroded by seawater; the method can be popularized in actual production very easily.

The invention belongs to the technical field of the pig feed, and relates to replacement gilt overhead price pellet feed for improving reproductive performance. The replacement gilt overhead price pellet feed is prepared by the following raw materials: high-quality corn, bean pulp, soybean, imported fish meal, skim milk powder, whey powder, coconut oil, glycine, complex vitamin, complex enzyme, luffa leaf slurry, fresh pineapple, fresh celery leaves, fresh peach leaves, fresh pear leaves, dipotassium glycyrrhizinate, Fritillaria thunbergii extract, galangin, and Radix Notoginseng P.E. The replacement gilt overhead price pellet feed has the characteristics of good palatability, balanced and comprehensive nutrition, easy absorption and conversion, and is capable of effectively improving the growth performance of the replacement gilt, improving the immunity function, improving the intestinal flora, effectively improving the estrus rate and pregnancy rate of the replacement gilt, and providing the good foundation for improving the born number and the farrowing quality.

The invention discloses a slow-release oxytocic agent for artificially inducing grouper gonad development and maturation, ovulation and ejaculation. 10%, 75-95% cholesterol, 1-19% cellulose; 1-3% vegetable oil is added to the formula components, mixed evenly, and cylindrical pills are made with a special mold. The invention is used to bury the grouper in the body to induce the maturation of the gonads to achieve ovulation and sperm discharge, so as to meet the needs of the grouper artificial seedling industrialization and large-scale breeding for seedlings.

The invention discloses an FLIP protein and new applications of encoded genes thereof. The new application of the invention refers to the application of the FLIP protein and the encoded genes thereof in blocking apoptosis induced by FADD and the application in inhibiting apoptosis of ovary granular cells and the application in promoting reproductive performance of animals. The FLIP protein and the encoded genes thereof can obviously inhibit apoptosis of the ovary granular cells induced by FADD, promote growth of oocyte, reduces follicular atresia and serve as potential medicines for improving reproductive performance of cows. The protein and the new applications of the invention provide relatively reliable and concrete experimental basis for increasing ovulation rate of dams and improving reproductive performance thereof, thus enjoying a wide application prospect in improving the reproductive performance of female animals.

The invention provides a method for facilitating ovulation of kelp female gametophytes. The method includes the steps: resuscitating kelp germplasm filaments in seawater, crushing kelp germplasms, andfiltering crushed liquid to obtain filament fragments with the lengths of 3-6 cells; performing aerated suspension culture on the filament fragments under the conditions of the illumination intensityof 6000+ / -300lx, the illumination cycle of L:D=12h:12h and the temperature of 15+ / -1 DEG C to collect eggs; culturing the collected eggs to obtain young kelp sporophytes, culturing the young kelp sporophytes, and performing culture by running water at the stage of young sporophytes of two rows of cells. According to the method, female kelp germplasms can ovulate in 11-13 days, ovulation time is shortened, ovulation rate is high, ovulation synchronization is good, ovulation rate within two days can reach 65-70%, and egg development rate can reach 70-80%. According to the built culture method of young sporophytes, the aberration rate of the young sporophytes can be reduced to 50-60%.

The invention provides an artificial breeding method of haliotis iris. The artificial breeding method comprises: promoting maturity of the haliotis iris with gonadal maturity of 20-30% to be 65-75% by adjusting an illumination period and light quality; and adjusting the illumination period again to promote the maturity of gonads so as to enable sperms or ova of the haliotis iris to be mature. The haliotis iris with mature gonads releases sperms or ova under stimulation of drying in the shade, H2O and NaOH. A parent abalone releases sperms or ova in a single spawning induction tank, the sperms can be added according to the number of the ova to increase the utilization rate of the ova and improve the fertility rate. Fertilized ova are put into a hatching pond for hatching after washing. Floating larvae after hatching are transferred into a nursery pond with an attachment substrate through a siphonage before metamorphosis so as to attach and metamorphose into juvenile abalones. The method provided by the invention is simple and convenient to operate, the haliotis iris is mature in gonad development, and relatively high in sperm or ovum releasing rate, and the fertility rate can reach 85%. In addition, the haliotis iris of which the gonad maturity is promoted to be 65-75% is cultivated in an environment at a temperature of 15 DEG C, thereby facilitating breeding all year round.

The invention discloses a breeding method for improving birth weight of Hough black pig piglets. The breeding method comprises the following steps of selecting a 6-month-old healthy breeding boar, feeding the breeding boar with a special feed for the breeding boar with the daily feeding amount of 2.5-3 kg, and freely drinking water; washing a breeding boar house with clean water every day, and spraying a compound disinfectant once every 2-3 days; selecting a 6-month-old healthy sow, feeding the sow with the special feed for the sow with the daily feeding amount of 2.5-3 kg, and freely drinkingwater; washing a sow house with clean water every day, and spraying the compound disinfectant once every 2-3 days; stroking the pig back and the periphery of the breast of the sow 10-20 minutes aftermeals every day from the 10th day to the 15th day before mating, and the stroking time of each sow is 4-8 minutes; starting the mating when the thickness of the backfat of the sow reaches 20-21 mm, the mating time is 3-4 days, and the mating frequency is 2-4 times; and stroking the sow continually for 40-50 days after mating is completed, and stop stroking in time when the sow do not adapt to stroking.

The invention provides a method for increasing the ovulation rate of sinonovacula constricta (Lamarck) parent oysters and guaranteeing ovulation synchronism. An inonovacula constricta (Lamarck) parent oyster ovulation device employed comprises a rectangular bottom frame, supporting rods, a shielding film and a light-dimming window, wherein small holes are drilled in the four corners of the bottom frame, the four supporting rods are inserted into the small holes to form a cuboid-shaped frame, the shading film as high as the supporting rods is put to surround the cuboid-shaped frame, and the light-dimming window is paved at the top of the cuboid-shaped frame; the bottom of the bottom frame is covered with a nylon net; an air inlet pipe is laid in the longitudinal direction of the bottom frame; three air stones are connected under the air inlet pipe. When the inonovacula constricta (Lamarck) parent oyster ovulation device is applied for ovulation, the light intensity in the light-dimming window is controlled at 200Lx, and therefore, the ovulation synchronism of the inonovacula constricta (Lamarck) is guaranteed, and meanwhile, the ovulation rate of the sinonovacula constricta (Lamarck) parent oysters is greatly increased and the shortcomings of the prior art are solved; meanwhile, the whole device is very convenient to assemble and disassemble, easy to clean and low in cost, and cannot be corroded by seawater; the method can be popularized in actual production very easily.

The invention relates to a medicinal composition for a high-androgen polycystic ovary syndrome. The medicinal composition comprises a Chinese medicinal composition and a chemical medicament, wherein the Chinese medicinal composition comprises the following medicinal materials: epimedium, caladium, fructus psoraleae, sliced deerhorn, sea horse, tortoise plastron and the like, and does not contain rhubarb and Chinese angelica. The medicinal composition can reduce the level of androgen and androgen receptors of polycystic ovary syndrome (PCOS) patients, improve the level of sex hormone binding globulin, reduce the level of free testosterone, improve the level of in-vivo estrogen further, improve the growth and development of follicles, and improve the ovulation rate and pregnancy rate of thepatients.

The present invention firstly provides a method for promoting kelp female gametophytes to discharge eggs. First, the kelp germplasm filaments are recovered in seawater, then the kelp germplasm is crushed, and the crushed liquid is filtered to obtain cells with a length of 3-6. The obtained filamentous body fragments; then the obtained filamentous body fragments were subjected to aerated suspension culture under the conditions of light intensity 6000±300lx, light cycle: L:D=12h:12h, and temperature 15±1°C to collect eggs; and then The collected eggs are cultured to obtain laminar sporozoites, and the laminar sporozoites are cultured, and the young sporophytes of the two rows of cells are cultured in flowing water. The method of the present invention enables the kelp female germplasm to ovulate in 11-13 days, shortening the ovulation time; and the ovulation rate is high and well synchronized, and the ovulation rate can reach 65-70% within 2 days; the egg development rate can reach 70-80% %. The established young sporophyte culture method can reduce the deformity rate of the young sporophyte to 50%-60%.

The invention discloses functional sow compound feed, and relates to the technical field of livestock feed. In parts by weight, the compound feed comprises 55-60 parts of corn, 15-20 parts of soybeanmeal, 5-10 parts of wheat middling, 5-10 parts of expanded soybean meal, 2-3 parts of fish meal, 1.5-2.5 parts of soybean oil, 1-2 parts of calcium hydrophosphate, 1-1.5 parts of stone powder, 0.3-0.5part of sodium chloride, 0.3-0.5 part of lysine, 0.1-0.2 part of a trace element premix, 0.1-0.2 part of a vitamin premix, 0.15-0.25 part of methionine, 0.1-0.2 part of high temperature resistant yeast, 0.05-0.1 part of microecological bacteria and 0.02-0.04 part of soy isoflavone. The functional sow compound feed can improve the estrus rate and ovulation rate of sows, increase the litter number,reduce the morbidity of the sows, effectively improve the economic benefits of breeding factories and promote the development of the pig industry.

The invention discloses saffron crocus steamed eggs for pregnancy and belongs to the technical field of food processing. The saffron crocus steamed eggs for pregnancy are specifically prepared from eggs, saffron crocus, bamboo leaves, rhizoma phragmitis, entada phaseoloides, fructus terminaliae billericae, alpine yarrow herbs, aleppo avens, tribulus terrestris, cortex ailanthi, Japanese ardisia herbs and astragalus membranaceus. The prepared saffron crocus steamed eggs for pregnancy have the functions of enriching blood, promoting blood circulation, regulating menstruation, relieving pain, smoothing the liver, regulating flow of qi, relieving depression and the like, can wholly integrate and adjust neuroendocrine gonad functions of the human body, increase the ovulation rate and the pregnancy rate, meanwhile, can relieve and eliminate the phenomena of poor sleep quality, a poor mental state, body weakness and the like frequently associated with unpregnant women, have a significant treating effect on female infertility, and have good application and popularization value.

The invention provides a traditional Chinese medicine composition for treating ovulatory dysfunction. The traditional Chinese medicine composition consists of the following components in parts by weight: 18-22 parts of seeds of Chinese dodder, 8-12 parts of fruits of Chinese wolfberry, 8-12 parts of framboise, 13-17 parts of cistanche salsa, 13-17 parts of morinda officinalis, 8-12 parts of herba epimedii, 8-12 parts of tangerine seeds, 8-12 parts of semen litchi, 4-6 parts of fennel, 8-12 parts of Chinese angelica, 8-12 parts of ligusticum wallichii, 8-12 parts of white paeony roots, 13-17 parts of herba leonuri, 8-12 parts of spatholobus suberctu stem, 8-12 parts of radix bupleuri, 4-6 parts of fructus aurantii and 4-6 parts of rhizoma cyperi. According to the clinical application, the traditional Chinese medicine composition for treating ovulatory dysfunction provided by the invention can increase the ovulation rate after a patient takes the composition for 3-6 course of treatments, also greatly increases the pregnancy rate, is especially remarkable in effect, is free of toxic or side effect, and is low in price.

The invention discloses a pharmaceutical composition for treating obese polycystic ovary syndrome. The raw materials are composed of 4-8 parts of ginger pinellia, 25-35 parts of fairy spleen, 8-12 parts of atractylodes rhizome, 16-24 parts of poria cocos, 10-14 parts of tangerine peel, 25-35 parts of Dipsacus, 8-12 parts of Cyperus cyperi, 16-24 parts of Salvia miltiorrhiza, 8-12 parts of Achyranthes knuckles, 8-12 parts of plantain seed, 4-8 parts of cinnamon, 4 parts of roasted licorice ‑8 servings. The medicinal materials selected for the pharmaceutical composition of the present invention are compatible, the prescriptions are refined, and the functions complement each other. The purpose is to promote dampness and eliminate phlegm, and it integrates expectorant, warming kidney, promoting qi, invigorating the spleen, promoting blood circulation, and tonifying. Significant; in terms of medicinal properties, they are compatible with each other, with less toxic and side effects.

The invention discloses a traditional Chinese medicine composition for treating infertility and a preparation method thereof. The traditional Chinese medicine composition for treating the infertilityis prepared from the following raw materials: fructus lycii, herba lycopi, rhizoma cyperi, rhizoma alpiniae officinarum, fructus tribuli, semen cuscutae, radix angelicae sinensis, radix paeoniae rubra, radix salviae miltiorrhizae and fluoritum. The traditional Chinese medicinal composition for treating the infertility has relatively good therapeutic effects and strong promotion effects, can promote female hormone secretion, improves ovulation rate of a patient and increases chances of fertilization.

The invention relates to a medicinal composition for a high-androgen polycystic ovary syndrome. The medicinal composition comprises a Chinese medicinal composition and a chemical medicament, wherein the Chinese medicinal composition comprises the following medicinal materials: epimedium, caladium, fructus psoraleae, sliced deerhorn, sea horse, tortoise plastron and the like, and does not contain rhubarb and Chinese angelica. The medicinal composition can reduce the level of androgen and androgen receptors of polycystic ovary syndrome (PCOS) patients, improve the level of sex hormone binding globulin, reduce the level of free testosterone, improve the level of in-vivo estrogen further, improve the growth and development of follicles, and improve the ovulation rate and pregnancy rate of thepatients.