Method for inducing Stichopus Japonicus Selenka to lay eggs in vivo

A technology for sea cucumber and parent ginseng, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of only 20-50% survival rate, only 15-30% metamorphosis rate of juvenile ginseng, and low spawning amount. , to reduce the cost of cultivation, improve the hatching rate, and improve the fertilization rate.

Active Publication Date: 2009-12-09
山东安源种业科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Although the literature points out that the injection of NGLWY amide in vivo can induce the release of sperm and eggs of sea cucumber and discloses that the injection volume of each individual is 0.1% (v / w), and the optimal concentration is 1nmol / L, but experiments have shown that injection of NGLWY alone can produce eggs The amount is small, for example, the average weight of mature parent ginseng with an average weight of 210 grams is only 430,000 eggs, the survival rate of planktonic larvae is only 20-50%, and the metamorphosis rate of immature ginseng is only 15-30%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] a. Preparation of oxytocic agent

[0017] Dissolve NGLWY amide purchased from Shanghai Sangon Biological Company with a purity greater than 98% in ultrapure water to prepare a 1 μmol / L mother solution, store in a -20°C refrigerator, and dissolve the mother solution in 0.5mol / L KCl seawater solution before injection In, make 1nmol / L oxytocic agent.

[0018] b. Select pro-parameters

[0019] In May, 28 ginsengs were collected from Dalian Xianyuwan Farm. The weight of the ginseng was between 200g and 260g, with an average weight of 224g. The collected ginseng was transported to the holding pond for 24 hours.

[0020] c. Injection of oxytocics

[0021] Use a 1ml medical disposable syringe to inject the oxytocic agent around the gonads of the sea cucumber. The injection dose (ml) is 0.1% (0.20-0.26ml) of the body weight (g) of the sea cucumber. In a plastic box with filtered seawater at a temperature of 17.5°C, the male sea cucumber began to crawl along the wall of the pl...

Embodiment 2

[0027] Except that step b is selected to participate in different embodiment 1, other steps are the same as embodiment 1.

[0028] In May, 110 ginsengs were collected from Shihe, Pulandian, Dalian. The weight of the ginsengs was between 240g and 350g, with an average weight of 274g. The collected ginsengs were temporarily raised in the holding pond for 72 hours, and no bait was fed during the holding period. The injection volume of oxytocic agent is 0.24~0.3ml.

[0029] The ejaculation and oviposition time is basically the same as in Example 1. A total of 54 brood ginseng ejaculated and 52 brood ginseng laid eggs. The egg production of female ginseng ranged from 2.92 million to 6 million, with an average egg production of 4.954 million .

[0030] Artificial fertilization and hatching, breeding, planktonic larvae cultivation and metamorphosis, attachment and cultivation of larvae are all the same as in Example 1. The survival rate of planktonic larvae is 74.6%, and the metamor...

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PUM

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Abstract

The invention discloses a method for inducing Stichopus Japonicus Selenka to lay eggs in vivo, which is characterized by simple operation, short time, synchronous egg laying, large discharge quantity and good quality of eggs, and capability of greatly improving induction rate, fertility rate and hatching rate; the method is implemented as the following steps: dissolving NGLWY acid amides in ultrapure water to formulate 1 mumol/L mother liquor, then dissolving the mother liquor in 0.5mol/L KCl sea water solution to prepare 1nmol/L inducer; selecting gonad mature parent Stichopus Japonicus Selenka with the weight being at least 200g; injecting the inducer with the ratio of volume and the weight of parent Stichopus Japonicus Selenka being 0.1% ml/g but less than 0.3ml into coelom from the back of the Stichopus Japonicus Selenka to perform in vivo induction; and collecting the eggs.

Description

Technical field: [0001] The invention relates to a method for inducing oviposition in the body of sea cucumbers, in particular to an in vivo oviposition method with simple operation, short time consumption, synchronous oviposition, large ovum discharge and good quality, which can greatly improve the efficiency of induced oviposition, fertilization rate and hatching rate. Method of inducing sea cucumber to lay eggs. Background technique: [0002] Sea cucumber has long been famous as a delicacy on the table of people in my country, Southeast Asia and other countries along the Pacific Ocean and the West Indian Ocean. Especially Chinese people in the world have always had the habit of eating sea cucumber as a "food tonic". Nowadays, sea cucumber has become more and more popular because of its rich nutritional value. more popular with people. The increase in demand has led to an increase in harvesting intensity, resulting in a sharp decline in the natural growth of sea cucumber r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 宋坚庞振国常亚青赵冲
Owner 山东安源种业科技有限公司
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