Method for inducing Stichopus Japonicus Selenka to lay eggs in vivo

Abstract

The invention discloses a method for inducing Stichopus Japonicus Selenka to lay eggs in vivo, which is characterized by simple operation, short time, synchronous egg laying, large discharge quantity and good quality of eggs, and capability of greatly improving induction rate, fertility rate and hatching rate; the method is implemented as the following steps: dissolving NGLWY acid amides in ultrapure water to formulate 1 mumol / L mother liquor, then dissolving the mother liquor in 0.5mol / L KCl sea water solution to prepare 1nmol / L inducer; selecting gonad mature parent Stichopus Japonicus Selenka with the weight being at least 200g; injecting the inducer with the ratio of volume and the weight of parent Stichopus Japonicus Selenka being 0.1% ml / g but less than 0.3ml into coelom from the back of the Stichopus Japonicus Selenka to perform in vivo induction; and collecting the eggs.

Description

Technical field: [0001] The invention relates to a method for inducing oviposition in the body of sea cucumbers, in particular to an in vivo oviposition method with simple operation, short time consumption, synchronous oviposition, large ovum discharge and good quality, which can greatly improve the efficiency of induced oviposition, fertilization rate and hatching rate. Method of inducing sea cucumber to lay eggs. Background technique: [0002] Sea cucumber has long been famous as a delicacy on the table of people in my country, Southeast Asia and other countries along the Pacific Ocean and the West Indian Ocean. Especially Chinese people in the world have always had the habit of eating sea cucumber as a "food tonic". Nowadays, sea cucumber has become more and more popular because of its rich nutritional value. more popular with people. The increase in demand has led to an increase in harvesting intensity, resulting in a sharp decline in the natural growth of sea cucumber r...

AI Technical Summary

Problems solved by technology

Although the literature points out that the injection of NGLWY amide in vivo can induce the release of sperm and eggs of sea cucumber and discloses that the injection volume of each individual is 0.1% (v / w), and the optimal concentration is 1nmol / L, but experiments have shown that injection of NGLWY alone can produce eggs The amount is small, for example, the average weight of mature parent ginseng with an average weight of 210 grams is only 430,000 eggs, the survival rate of planktonic larvae is only 20-50%, and the metamorphosis rate of immature ginseng is only 15-30%.