Molecular marker for identifying fertility of hybrid progeny of common wild rice and cultivated rice and application of molecular marker
A technology of molecular markers and fertility, applied in the biological field, can solve problems such as limiting the effective use of good genes in wild rice
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[0053] Example 1. Application of Rice Fertility Molecular Markers in Detecting the Fertility of Offspring of Rice Distant Hybrids
[0054] 1. Molecular markers related to the fertility of rice distant hybrids
[0055] The present invention has discovered a SNP site that can be used to detect the fertility of rice distant hybrid offspring, which is recorded as a rice fertility molecular marker. In rice, the molecular marker is T or C, and the SNP site is corresponding to The 2099th nucleotide of sequence 1 in the sequence listing. The rice whose SNP site is T in both chromosomes is recorded as TT genotype rice, the rice whose SNP site is C in both chromosomes is recorded as CC genotype rice, and the SNP site in Rice with T in one chromosome and C in the other chromosome was designated as TC genotype rice.
[0056] Design the primer pair A1 capable of detecting this site. A1 is composed of two single-stranded DNAs named P1 and P2. The reverse complementary single-stranded DNA...
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