Paddy rice hybrid embryo sac fertility gene and use thereof

A technology of hybrid embryos and rice, applied in the field of plant genetic engineering, can solve problems such as negative effects on indica and japonica hybrid fertility, death of japonica female gametes, and reduced hybrid fertility

Active Publication Date: 2013-12-04
HUAZHONG AGRI UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Indica-type ORF5 can lead to the death of japonica-type female gametes (Chen et al.2008, A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indica–japonica hybrids in rice.Proc Natl Acad Sci USA 105(32) :11436-41), but the transformation of ORF5 of wide-compatibility variety 02428 into japonica rice did not protect japonica female gametes (Ji Q, et al.2010, Two sequence alterations, a 136bp InDel and an A / C polymorphic site, in the S5 locus are associated with spikelet fertility of indica-japonica hybrid in rice. J Genet Genomics.37(1):57-68)
These results indicate that ORF5 has a negative effect on the fertility of indica and japonica hybrids, and only reduces the fertility of hybrids

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paddy rice hybrid embryo sac fertility gene and use thereof
  • Paddy rice hybrid embryo sac fertility gene and use thereof
  • Paddy rice hybrid embryo sac fertility gene and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation and cloning of DNA fragments comprising S5 ORF3 gene segments

[0027] 1. Identification of rice hybrid embryo sac fertility gene ORF3 by map-based cloning

[0028] In the present invention, three parents were used when preparing the mapping population: 02428, Nanjing 11 (Nanjing11) and Balilla. 02428 (from Jiangsu Academy of Agricultural Sciences, China) is a japonica wide-compatibility variety selected by Jiangsu Academy of Agricultural Sciences. Two japonica rice varieties, Yunnan Crab Valley and Shanghai Jibang Rice, were treated with radiation mutagenesis and treated with high light efficiency. The selected materials were crossed and bred from the offspring (Zou Jiangshi et al., Preliminary utilization of the wide-compatibility selection line 02428 in the cross between indica and japonica subspecies. Chinese Agricultural Sciences, 1989, 22: 6-14). Nanjing 11 (abbreviated as NJ11, from Jiangsu Academy of Agricultural Sciences, China) is a medi...

Embodiment 2

[0051] Example 2: Construction of a near-isogenic line at the S5 locus

[0052] In order to detect the effect of transgenic NJ11 ORF3 on seed setting rate in other backgrounds, it is necessary to construct a near-isogenic line at the S5 locus. Use Nanjing 11 (abbreviated as NJ11) and Dular (variety introduced from India by our country. For literature, see: Anonymous, Artificially Induced Mutation of Quantitative Traits of Rice. Northern Rice, 1977, 5:24. Gu Minghong et al., Wide Compatibility Genes of Rice Varieties, etc. Genetic analysis of positional relationship. Chinese Agricultural Sciences, 1993, 1:13-21)) S5 chromosome segment donor, and Balilla as recipient to construct a near-isogenic line. See Example 1 for information on Nanjing 11 and Balilla. The molecular markers used in the construction of near-isogenic lines are shown in Table 3, and the specific operation method can be found in the literature (Wang et al, 2005. Identification and confirmation of three neutral...

Embodiment 3

[0054] Example 3: Effect analysis of NJ11 ORF3 (ORF3+) transgene on seed setting rate of hybrid offspring

[0055] Ballilla (S5-NJ11 / NJ11)×Balilla NJ11 ORF3 Analysis of seed setting rate of hybrid offspring (Table 4). Balilla(S5-NJ11 / NJ11) means that NJ11 is a near-isogenic line in which NJ11 is the donor of the S5 segment, and Balilla is the recipient, and is homozygous for NJ11 in the S5 segment. A near-isogenic line that is heterozygous for Balilla / NJ11 at the S5 segment is denoted as Balilla(S5-Balill / NJ11), and so on. Cross F1 has transgene negative / positive segregation. The average seed setting rate of the negative F1 plant Balilla (S5-Balilla / NJ11) was 50.3±0.6; the positive F1 plant Balilla (S5-Balilla / NJ11) NJ11 ORF3 The average seed setting rate was 71.5±0.6. The difference in seed setting rate between negative and positive F1 plants reached a very significant level (P value of t test was 0.00), indicating that transgenic NJ11 ORF3 had a very significant increase...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of plant gene engineering and especially relates to isolation and cloning and functional identification of a paddy rice S5-site hybrid embryo sac fertility gene ORF3 and a use of the paddy rice S5-site hybrid embryo sac fertility gene ORF3 in paddy rice breed improvement. The gene ORF3 codes an Hsp70 albuminoid and has functions of folding and transferring cell proteins and transducing stress signals. Different alleles of the gene ORF3 have insertion / deletion variation of 13bp in a coding zone, and the length of the dominant allele ORF3+ is 13bp more than that of the recessive allele ORF3-. A nucleotide sequence of the recessive allele ORF3- is shown in the formula of SEQ ID NO: 1 and a nucleotide sequence of the dominant allele ORF3+ is shown in the formula of SEQ ID NO: 3. In a S5 oryza sativa L. ssp. Indica / oryza sativa L. ssp. Japonica hybrid, the dominant allele ORF3+ can protect female gametes so that a maturing rate of the S5 oryza sativa L. ssp. Indica / oryza sativa L. ssp. Japonica hybrid is improved. The paddy rice hybrid embryo sac fertility gene has an important meaning for using oryza sativa L. ssp. Indica / oryza sativa L. ssp. Japonica hybrid vigor.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering. It specifically relates to the isolation and cloning of a hybrid embryo sac fertility gene ORF3 located at the S5 site of rice, its function verification and its application in rice variety improvement. Background technique [0002] Asian cultivated rice (Oryza sativa L.) has two subspecies: the indica subspecies (ssp. indica) and the japonica subspecies (ssp. japonica). Indica-japonica inter-subspecies hybrids have stronger heterosis than inter-species hybrids, but the low fertility of indica-japonica inter-subspecies hybrids limits the utilization of their heterosis. Indica-japonica interspecies hybrids are often sterile or semi-sterile (Kato S, Kosaka H, ​​Hara S (1928) On the affinity of rice varieties as shown by fertility of hybrid plants. Bull Sci Fac Agric Kyushu Univ, 3: 132-147; Oka HI (1988) Origin of cultivated rice. Scientific Societies Press, Tokyo, Japan pp 181–...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/57C12N9/50C12N15/11C12Q1/68A01H1/04
Inventor 张启发杨江义欧阳亦聃杜红毅
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap