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Oryza sativa male sterility gene OsFIGNL1 (Oryza sativa Fidgetin-like 1) and application thereof

A male sterility gene and gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem that the OsFIGNL1 gene has not yet been reported, and achieve the effect of great development and utilization value and broad market application prospects.

Active Publication Date: 2017-09-19
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In rice, the related research of OsFIGNL1 gene has not been reported so far

Method used

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  • Oryza sativa male sterility gene OsFIGNL1 (Oryza sativa Fidgetin-like 1) and application thereof
  • Oryza sativa male sterility gene OsFIGNL1 (Oryza sativa Fidgetin-like 1) and application thereof
  • Oryza sativa male sterility gene OsFIGNL1 (Oryza sativa Fidgetin-like 1) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Identification of rice male sterile mutant fignl1 phenotype

[0039] Rice male sterile mutant fignl1 is 60 Co-γ irradiated indica rice variety Zhonghui 8015 (ZH8015) produced sterile mutants. The fignl1 mutant had no significant difference with the wild type in the vegetative growth stage, and there was no abnormality in plant height, tillering and other plant type traits. During the reproductive growth stage, there was no significant difference in the palea, pistil and paddles between wild-type florets and mutant florets. However, the mature anthers of the wild type Zhonghui 8015 were hypertrophic, and the anthers were yellow and plump. 2 -KI stained the mature pollen, it can be observed that the pollen of the mutant is not dyed, and the shape is irregular. Observing the pollen grains in different fields of view, it is found that the pollen of the mutant is completely iodized, while the pollen of the wild type is normally stained. At the maturity stage of ...

Embodiment 2

[0040] Example 2 Observation on semi-thin section of anther development process of fignl1 mutant

[0041] The ears of rice fignl1 mutant and wild type grown in different development stages were taken in the field, and a small opening was cut with scissors for the larger ears to facilitate the infiltration of osmotic fluid. The sample was fixed in 50% FAA fixative (50% ethanol: formaldehyde: glacial acetic acid = 90: 5: 5), and slowly evacuated for 30 minutes with a vacuum pump. The sample sank to the bottom of the bottle, and the newly configured 50% FAA fixative was replaced. Let stand at room temperature for 16-24 hours. In FAA fixative, samples can be stored for up to three months. For long-term storage, the fixative can be replaced with 70% ethanol and stored at room temperature. According to the floret length, the materials were stored separately. The palea of ​​the florets were removed with tweezers in 70% alcohol and stored in penicillin vials. Alcohol gradient dehy...

Embodiment 3

[0042] Example 3 Scanning electron microscope observation of mature anthers of fignl1 mutant

[0043] Place the mature anthers of the wild-type Zhonghui 8015 and the fignl1 mutant in 2.5% glutaraldehyde, slowly vacuum until the sample sinks to the bottom of the tube, fix in a 4°C refrigerator overnight, and operate as follows in the next morning: add the fixative Pour off, the anther sample is washed three times with phosphate buffer (0.1M, pH7.0), each 20 minutes; Fix the sample with 1% starvation acid solution for 1.5 hours, the anther sample is dyed black; The sample was washed three times with phosphate buffer (0.1M, pH 7.0), each time for 20 minutes; the sample was dehydrated through gradient 30%, 50%, 70%, 80%, 90% and 95% alcohol dehydration for 10 - After 15 minutes, treat with 100% ethanol twice, 20 minutes each time; treat the sample with isoamyl acetate overnight, C0 2 Critical point drying, after coating, the sample was observed and photographed under a (TM-1000Hi...

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Abstract

The invention relates to an oryza sativa male sterility gene OsFIGNL1 (oryza sativa Fidgetin-like 1) and an application thereof. The nucleotide sequence of the gene OsFIGNL1 is shown as SEQ ID NO:1. One function lost mutant of OsFIGNL1 is obtained through <60>Co-gamma mutagenesis, and the mutant in a field planting environment shows the following characteristics: vegetative growth of plants are normal, male plants are sterile, pollen is 100% typically abortive, and development of female gametes is not affected. Meiosis squashing operation shows that chromosome behaviors are severely affected by function loss of OsFIGNL1 during meiosis, and developmental defects of microspores are caused finally. The mutant can be applied to breeding of new dominant varieties of oryza sativa hybrids. The gene OsFIGNL1 can serve as a functional gene for regulating meiosis of oryza sativa to be applied to oryza sativa heterosis creation work in future and has huge development and utilization value and broad market application prospect.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to rice male sterility gene OsFIGNL1 and its application. Background technique [0002] The male sterility of plants often shows that the pistil develops normally, and the male gametes cannot perform normal functions when they are aborted. Rice male sterile materials have great application value in the utilization of rice heterosis and the cultivation of hybrid rice. Most of the male sterile materials are caused by mutations of recessive nuclear genes, and their sterility is not affected by the environment. Genetically stable; and the vast majority of them are controlled by a single gene. The cloning of the rice recessive genetic sterile gene and the functional study of its expression regulation will help us to understand the process of male reproductive development in rice, and can also lay a foundation for the cultivation of new male sterile line materials and the creatio...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/63C12N15/82C12N15/11A01H5/00
CPCC07K14/415C12N15/113C12N15/8289
Inventor 程式华张沛沛曹立勇张迎信孙廉平杨正福斯努姆波恩·斯蒂普恩吴玮勋
Owner CHINA NAT RICE RES INST
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