Dark vein gene obv and application thereof

A gene and dark vein technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of restricting gene application and unclear function, and achieve the effect of broad market application prospect and huge development and utilization value

Pending Publication Date: 2021-10-29
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the gene has not been cloned yet, and its function is

Method used

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  • Dark vein gene obv and application thereof
  • Dark vein gene obv and application thereof
  • Dark vein gene obv and application thereof

Examples

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Embodiment Construction

[0040] The present invention will be described in further detail below through specific examples.

[0041] 1. Identification of bright and dark veins in tomato leaves.

[0042] The cotyledons of the two genotype tomato materials already exhibited different veins at budding, making it easier to distinguish between light and dark veined phenotypes as true leaves developed. There is not much difference in the size, thickness and shape of the leaves between the two, but there is a significant difference in the light and dark phenotype of the veins. Under direct sunlight, the obvious difference can be observed with the naked eye, such as figure 1 shown.

[0043] In order to further observe the difference between the bright and dark veined materials, the cross-sections of the leaf veins of the bright and dark veined materials were observed with paraffin sections. It can be clearly seen that the extension of the vascular bundle sheath in the veins of the bright veined materials is m...

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Abstract

The invention provides a novel dark vein gene obv. The dark vein gene is a zinc finger transcription factor for coding a C2H2L structural domain, and the gene is mainly expressed in vascular bundles in veins and surrounding fence tissues and is positioned in a cell nucleus. The dark vein gene obv provided by the invention can be used for improving the chlorophyll content, photosynthetic rate and stomatal conductance of leaf veins and increasing a chloroplast quantity. The gene obv provided by the invention participates in regulation of chloroplast development and photosynthesis of tomato leaves, and a theoretical basis and a technical support can be provided for genetic improvement on high-photosynthetic-efficiency tomatoes. The gene obv provided by the invention can improve the yield of tomatoes and the content of soluble solids, is of great significance in the high quality and high yield of the tomatoes, can be applied to the cultivation of new tomato varieties of heterosis and has huge development and utilization values and wide market application prospects.

Description

technical field [0001] The invention relates to a dark vein obv gene and its application. The present invention adopts the method of forward genetics, clones to control tomato dark vein gene obv, knocks out obv gene through CRISPR / Cas9 knockout technology, makes bright vein tomato leaf appear dark vein; and through overexpressing obv gene, makes dark vein tomato Bright veins appeared in the leaves, which confirmed the function of the gene. It also relates to a carrier containing the gene and homologous genes of other species, and involves using the gene or its functional analogue to regulate changes in plant leaf veins and chlorophyll content. Background technique [0002] Leaf is the main organ for photosynthesis and respiration in plants. Photosynthesis is the basis of plant growth and development, and also the main determinant of yield and quality. Leaf-related traits are one of the key traits for crop genetic improvement. [0003] Tomatoes are native to the Andes in So...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/12A01H6/82
CPCC07K14/415C12N15/825C12N15/8269C12N15/8261
Inventor 刘磊鹿京华李君明李鑫杜永臣国艳梅黄泽军王孝宣舒金帅
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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