328 results about "Gene Deletions" patented technology

The invention discloses an indirect ELISA kit for distinguishing porcine actinobacillus pleuropneumoniae wild virus infection and vaccine immunity and application, and belongs to the technical field of biology. According to the indirect ELISA kit, screened ApxIVA truncated protein serves as a coating antigen, the amino acid sequence of the ApxIVA truncated protein is shown in SEQ ID NO.3, the ApxIVA truncated protein can be subjected to soluble expression in escherichia coli, only one His tag and one S tag are carried, and the structure and the function of the ApxIVA truncated protein are close to those of natural ApxIVA. The pxIVA truncated protein is used as an antigen, an indirect ELISA method and kit for distinguishing APP wild strain infection and gene deletion vaccine immunity are established, the kit has the advantages of high specificity, low background value, low cost and short detection time, and the kit has the advantages of improving animal husbandry APP diagnosis accuracy and improving animal husbandry APP diagnosis accuracy. And the method has important significance in the aspects of optimizing prevention and control measures and guaranteeing animal health and production benefits.

The invention belongs to the field of gene engineering, and relates to a gene-deleted recombinant toxoplasma gondii strain vaccine and a construction method thereof, in particular to a gene-deleted recombinant toxoplasma gondii strain, a drug and / or a biological product containing the gene-deleted recombinant toxoplasma gondii strain, a construction method and application of the gene-deleted recombinant toxoplasma gondii strain. The toxoplasmosis vaccine with attenuated activity developed based on the recombinant toxoplasmosis strain is suitable for immunoprophylaxis of toxoplasmosis of various mammals, can block transmission of toxoplasmosis to people and other animals, and is especially suitable for immunoprophylaxis of cat toxoplasmosis infection and / or toxoplasmosis. The vaccine is easy to produce, high in safety, excellent in efficacy, long in immunization duration, capable of distinguishing vaccine immunization from natural infection, free of pollution to animals and the environment, capable of effectively reducing residues of toxoplasma oocysts in the environment and reducing the risk that people are infected with toxoplasmosis, and has huge application potential and important public health significance.

Disclosed herein are methods, compositions, and devices for use in diagnosis and treatment of cancer. The methods include sequencing a panel of regions in cell-free nucleic acid molecules and detecting one or more biomarkers that are indicative of a cancer.

The invention discloses a rice OsFb34 gene as well as an encoding protein and application thereof, and relates to the technical field of plant genetic engineering. The CDS nucleotide sequence of the rice OsFb34 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the rice OsFb34 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing a knockout vector of a rice OsFb34 gene; transforming the constructed knockout vector into rice; and cultivating and screening to obtain a transgenic rice plant with reduced rice grain length, increased grain width and reduced length / width ratio. The mutants of osfb34-1 and osfb34-2 both show phenotypes that the grain length is reduced and the grain width is increased, so that the ratio of the length / width ratio is reduced. The result shows that the OsFb34 gene deletion can cause the reduction of the grain length and the increase of the grain width of the rice, and further cause the reduction of the length / width ratio.

The invention provides dsRNA (double-stranded ribonucleic acid) targeting a BcMucin gene and application of the dsRNA in preparation of a preparation for preventing and treating gray mold. The dsRNA is obtained by taking a coding sequence of a target gene BcMucin as a template through manual design and in-vitro synthesis. The dsRNA with the BcMucin as the target can be used for effectively inhibiting the occurrence of the botrytis cinerea. Experiments prove that after the deletion of the target gene BcMucin provided by the invention, botrytis cinerea hyphae grow slowly, the pathogenicity to tomatoes is completely lost, and the target gene BcMucin is a key gene for botrytis cinerea growth and pathogenicity regulation. The dsRNA provided by the invention can effectively interfere the expression of a target gene BcMucin in botrytis cinerea so as to inhibit the growth and development of the botrytis cinerea and the infection of the botrytis cinerea on host plants and reduce the biomass of the botrytis cinerea on scabs. The dsRNA provided by the invention can be used for RNA pesticide development based on RNAi, is applied to effective prevention and treatment of gray mold, and has a good market application prospect.

The invention discloses a streptococcus suis type 2 gene deletion strain as well as a construction method and application thereof, and belongs to the technical field of biological products. According to the invention, the B9H0105210 gene in the genome of the streptococcus suis serotype 2 SC19 is deleted in a homologous recombination manner for the first time, the hydrogen peroxide and nitric oxide stress resistance of the constructed streptococcus suis serotype 2 gene deleted strain is reduced, and the pathogenicity to mice is obviously weakened; moreover, the growth performance of the streptococcus suis type 2 gene-deleted strain is not greatly different from that of a wild strain SC19, the condition of gene recovery does not exist after continuous passage, and the streptococcus suis type 2 gene-deleted strain has an application prospect as an attenuated live vaccine, has a good immune protection effect on different genotypes of streptococcus suis such as the streptococcus suis type 2 strain SC19 and streptococcus suis type 2 P1 / 7, and has a good application prospect. And a certain cross immune protection performance is achieved.

The invention discloses a colloidal gold test strip for distinguishing porcine actinobacillus pleuropneumoniae wild virus infection and vaccine immunity and application. According to the test strip, ApxIVA truncated protein with the amino acid sequence shown as SEQ ID NO.3 serves as an antigen, the truncated protein can be subjected to soluble expression in escherichia coli, and the structure and the function of the truncated protein are close to those of natural apxIVA. The test strip comprises a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and a gold-labeled chicken IgY antibody, and a goat anti-chicken IgY antibody and apxIVA truncated protein are fixed on a C line and a T line of the NC membrane. The method can be used for distinguishing APP wild strain infection and gene deletion seedling APP-HB-04M immune serum samples.

The invention relates to the technical field of gene engineering, and particularly discloses streptococcus agalactiae WC1535 delta cps delta hylB as well as a construction method and application thereof. The streptococcus agalactiae WC1535 [delta] cps [delta] hylB is preserved in Guangdong Microbial Culture Collection Center on February 28, 2025, the preservation number is GDMCC NO.65955, and the address of the preservation unit is the 5th building, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. Compared with a traditional single-fragment deletion method, the toxicity of the deletion strain is not prone to being enhanced, and compared with a traditional injection immune vaccine, the gene deletion strain is used for preparing the tilapia oral live vaccine, the immune operation is simpler, and more labor cost is saved.

The invention provides a Brucella immunosuppressive gene BtpB deletion vaccine as well as a preparation method and application thereof, and belongs to the technical field of vaccine strains. The invention provides a suicide plasmid for knocking out a BtpB gene in a bovine brucella A19 vaccine strain and a gene deletion strain prepared on the basis of the suicide plasmid, and the BtpB gene deletion is performed on the brucella A19 strain so as to achieve the purpose of modifying the A19 vaccine. The A19 delta BtpB deletion strain constructed by the invention can reduce damage to host visceral organs, can induce a mouse to generate a high-titer Brucella specific antibody IgG, can better induce the mouse to generate Th1 type cytokines, and can provide better protective efficacy for the mouse than an A19 vaccine strain, so that the A19 delta BtpB deletion strain can be used as a vaccine for the mouse. The A19 delta BtpB deletion strain is expected to become a novel Brucella gene deletion vaccine candidate, and a theoretical basis and a data support are provided for developing a novel Brucella gene deletion vaccine.

The present invention provides a 5-hydroxytryptophan production strain and a construction method and application thereof. The strain has a clear genetic background and is effective in producing 5-hydroxytryptophan. E.coli HTP11 Subtilis subtilis spores pureF bsu K316Q Mutated gene, upregulated strain E.coli HTP11 of guaB , guaA , gmk , ndk The transcription level of the gene was increased by heterologous introduction of the gapX Gene, missing aromatic amino acid transporter serB , tnaB , aroP Gene, up-regulated inner membrane efflux transporter yhjV The transcription level of the gene was increased and electroporated into the pTm-serB plasmid, which could stably provide 5-hydroxytryptophan, the direct precursor of 5-hydroxytryptophan, and had a high efficiency of 5-hydroxytryptophan output.

The invention discloses a recombinant adenovirus based on 33 protective genes of Brucella, 33 most important protective antigen genes are screened from 89 whole genes of Brucella through a bioinformatics technology, and are recombined into a human type 5 replication-deficient adenovirus vector through a genetic engineering technology. Therefore, the recombinant adenovirus vaccine based on 33 protective genes of Brucella is obtained. The invention effectively overcomes the defects of virus dispersion, virulence recombination, virulence reversion, virulence residue and the like of attenuated vaccine strains such as conventional brucella vaccine strains, gene deletion vaccine strains and the like, is a safe and effective vaccine, has the effect reaching the brucella vaccine evaluation standard formulated by the world animal hygiene organization OIE, and can be applied to prevention of brucellosis.

The invention discloses an application of an MDH2 gene and an inhibitor thereof in resisting ZEA toxin, and relates to the technical field of biology. An MDH2 gene deletion cell line is obtained through CRISPR gene editing, and compared with a wild type MDH2 gene expression cell, under the influence of ZEA, the cell activity of the MDH2 gene deletion cell line is remarkably improved, and the cell apoptosis rate is remarkably reduced. In addition, the LW6 inhibitor is used for inhibiting the MDH2 activity of wild type IPEC-J2 cells, and the tolerance of the cells to ZEA can also be improved. Therefore, through knockout, mutation or activity inhibition of the MDH2 gene, the ZEA resistance of people, animals and plants can be improved. The invention provides a potential solution way for solving untoward effects caused by ZEA (Zinc Ethylenediamine).

Provided herein are diagnostic methods for identifying cancers suitable for treatment with an MTA-cooperative PRMT5 inhibitor, such as antibody-based methods and nucleic acid-based methods. The patients carry mutations that adequately sensitize cancer cells to an MTA-cooperative PRMT5 inhibitor, such as complete and certain partial MTAP gene deletions. Also provided are methods of treating the patients selected by such diagnostic methods.

The invention relates to the technical field of biology, in particular to application of glucoside hydrolase FsGH28c and a coding gene thereof in regulation of strain pathogenicity. The invention provides a glucoside hydrolase coding gene FsGH28c related to development, carbon source utilization and pathogenicity of fusarium solani, and after the FsGH28c gene is knocked out, the sporulation quantity is reduced, spore development is deformed, and the utilization rate of a carbon source (especially pectin) is reduced. In addition, the colonization amount of the FsGH28c gene deletion mutant in host pepper root fungi is reduced, and the pathogenicity is reduced. The FsGH28c gene can positively regulate the pathogenicity of fusarium solani, and can be used as an important candidate gene for research on the pathogenesis of fusarium solani.

Genome sequencing identified a new gene associated with a cardiac arrhythmic disease, TAXI -binding protein 3. From cell line models and a conditional knock-out mouse models of the gene deletion, it was found that a specific molecule, transient receptor potential vanilloid 4 (TRPV4), is up-regulated and is associated with calcium mediated arrhythmic depolarizations.

The invention discloses a PDCoV JS2021-LX strain NS6 gene deletion recombinant virus and a construction method thereof, on the basis of PDCoV JS2021-LX strain infectious clone successfully constructed by a BAC system, the upstream and downstream of the NS6 gene are cut by adopting a CRISPR / Cas9 technology, then the original NS6 gene segment is replaced by green fluorescent protein EGFP through a homologous recombination mode, an NS6 gene deletion recombinant plasmid is obtained, and the NS6 gene deletion recombinant virus is constructed. And after the LLC-PK1 cell is transfected, the recombinant virus without the NS6 protein is rescued. The recombinant virus with the PDCoV NS6 gene deleted is successfully rescued, the virus replication level of the recombinant virus and the virus is rescued through in-vivo and in-vitro evaluation, and pig experiment results show that the pathogenicity of the recombinant virus is reduced, and the recombinant virus has the potential of serving as a candidate strain of an attenuated live vaccine.

The invention provides a gntR17 gene deleted strain M5 delta gntR17 as well as a construction method and application thereof, and belongs to the technical field of biology. The M5 delta gntR17 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 4, 2024, and the preservation number is CGMCC NO.46061. The M5 delta gntR17 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC). It is found that the gntR17 gene is a related gene affecting the virulence of the brucella, the virulence of the brucella can be weakened by deletion of the gntR17 gene, the safety is improved, the survival ability of the brucella in cells and a mouse body can be remarkably reduced after deletion of the gntR17 gene, and the gntR17 gene has potential application value. Compared with the original brucella M5 strain, the M5 delta gntR17 strain constructed after the gntR17 gene is knocked out has the advantages that the virulence is weakened, the safety is high, the immune protection similar to that of an M5-90 delta 26 vaccine is realized, and the strain can be used as a candidate attenuated live vaccine strain for preventing animal brucellosis.

Disclosed herein are methods, compositions, and devices for use in diagnosis and treatment of cancer. The methods include sequencing a panel of regions in cell-free nucleic acid molecules and detecting one or more biomarkers that are indicative of a cancer.

The invention relates to the technical field of viruses, in particular to a construction method and application of an African swine fever virus strain. According to the invention, the three virulence genes MGF110-9L, EP402R and I73R of the African swine fever virus are taken as targets for the first time to construct a virus strain with deletion of the three virulence genes, and the virus strain is found to be capable of effectively reducing the virus toxicity but retaining better immunogenicity, can improve the immunity of an organism to the African swine fever virus, and has a relatively great application prospect.

The present application relates to the field of veterinary diagnostic technology, and specifically to a probe composition, a primer composition, and a triple fluorescence PCR kit and related methods for differential diagnosis of three genes of different strains of African swine fever composed of the above-mentioned probe composition and primer composition. The kit includes primer probes specific to the B646L gene, the CD2v gene, and the I177L gene. The present application designs fluorescent PCR primers and probes for the B646L gene, the CD2v gene, and the I177L gene, respectively, and establishes a triple fluorescence PCR detection method, which can quickly identify African swine fever virus strains and gene-deficient strains. At the same time, it has no cross-reaction with swine fever virus, porcine reproductive and respiratory syndrome virus, pseudorabies virus, porcine epidemic diarrhea virus, porcine circovirus, porcine parvovirus, healthy whole blood, oropharyngeal swabs, nasal swabs, anal swabs, environmental swabs, pig tissues, etc., and has good specificity.

The invention relates to a dual ERA-LFD kit for visually and rapidly detecting salmonella typhimurium or a single-phase variant thereof, a primer group and application of the dual ERA-LFD kit and the primer group, and aims at solving the problems that an existing detection method is complex in operation, long in time consumption and depends on professional equipment, and primer cross interference, high false positive rate and incapability of distinguishing the single-phase variant caused by the fact that dual ERA primer design is not standard. Screening to obtain a specific primer probe combination aiming at the fljB gene deletion region and the STM4495 gene; a dual ERA-LFD detection system is constructed, and double targets are synchronously amplified under a constant temperature condition; visual interpretation is realized through a double-detection-line color development mode of the transverse flow test strip. The detection specificity can be remarkably improved, and false positive is effectively avoided; the accurate distinguishing between the salmonella typhimurium and the single-phase variants is realized; the detection process is rapid and simple, does not need professional instruments and personnel, and is suitable for on-site rapid screening; the detection cost is obviously reduced and the basic application feasibility is improved.

The invention provides a deletion strain and a supplementation strain of related genes of Aeromonas dakara PhoBR and QSS as well as a construction method and application of the deletion strain and the supplementation strain, a deltaahyR, deltaluxS and deltaqseB gene deletion strain and a supplementation strain as well as a deltaphoB deltaahyR, deltaphoB deltaluxS and deltaphoB deltaqseB double-gene deletion strain are successfully constructed, it is proved that the PhoBR can directly regulate and control the related genes ahyR, luxS and qseB of the QSS in the Aeromonas dakara, negative regulation and control on the ahyR and the luxS and positive regulation and control on the qseB are shown, the QSS related genes can be directly regulated and controlled in the Aeromonas The regulation and control are crucial to the survival and environment adaptation of bacteria. Besides, through a related bioinformatics method, possible binding domains of part of PhoB and three QSS related genes ahyR, luxS and qseB have been predicted, and a scientific basis is further provided for immune prevention and treatment of Aeromonas dakara.

The invention belongs to but not limited to the technical field of genetic engineering technology, and discloses a construction method and application of a BspE gene deletion strain of Brucella effect protein, the BspE gene deletion strain is constructed by using homologous recombination and SacB reverse screening technology, Brucella abortus A19 vaccine strain liquid is used as a template, PCR amplification is carried out to obtain upstream and downstream homologous arms of BspE gene, and the BspE gene deletion strain is constructed by using the upstream and downstream homologous arms of the BspE gene. The method comprises the following steps: carrying out fusion PCR (Polymerase Chain Reaction) amplification on upstream and downstream homologous arms of a BspE gene subjected to gel recovery, and connecting and transforming an obtained fusion fragment and a pUC19-SacB plasmid to obtain a recombinant plasmid pUC19-delta BspE. The invention relates to a bovine brucella BspE deletion vaccine strain, which is characterized in that a plasmid is obtained, the plasmid is electrically transformed into a bovine brucella A19 competent cell, and the positive transformant is a bovine brucella BspE deletion strain through kanamycin resistance and sucrose pressure screening, PCR (polymerase chain reaction) identification and sequencing, and the deletion vaccine strain improves the levels of antibacterial cytokines and specific brucella antibody IgG, and can be used for preparing the bovine brucella BspE deletion vaccine strain. The Brucella attenuated vaccine strain is a novel Brucella candidate attenuated vaccine strain and has a good market prospect.

The invention discloses a gene for regulating and controlling wheat plant height and sub-panicle internode length and application of a coding protein of the gene, and belongs to the technical field of biology. The gene capable of regulating and controlling the plant height and the sub-panicle internode length of the wheat is TaSVP-6B (SEQ ID NO: 1), the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 26, and the application of the TaSVP-6B gene and the protein coded by the gene in regulating and controlling the plant height and the sub-panicle internode length of the wheat specifically comprises the following steps: deleting the TaSVP-6B gene in a target wheat genome by utilizing a gene editing technology; or the expression of the protein shown as SEQ ID NO: 26 of the target wheat is inhibited, so that the dwarf wheat with shortened plant height and sub-spike internode length is obtained. The gene has the beneficial effects that a gene resource is provided for cultivating new crop varieties adapting to different ecological environments, and meanwhile, the gene has important theoretical and practical significance for knowing the formation of crop plant types.

The invention belongs to the technical field of biology and new medicine, and discloses a method for constructing a myxobacteria expression chassis with genetic stability and application of the myxobacteria expression chassis. Through gene deletion and overexpression analysis, it is verified that the spontaneous mutation rate of a strain can be reduced by deletion of a TLS pathway and overexpression of RecA, and a myxobacteria strain 2K7 with genetic stability is further constructed on the basis. The spontaneous mutation rate of the new strain is reduced by 11.5 times, and the transformation capability of growth plasmids of the strain is obviously improved. According to the research, the genetic stability of the myxobacteria is improved for the first time through a repair way after modification and replication, and the research method and the research result are innovative.

The invention relates to an establishment method and application of in-vitro intestinal inflammation models of mice of different ages, and relates to the technical field of biology. According to the invention, young, adult, middle-aged and old wild type (WT) and TLR4 gene deletion type (TLR4 <- / ->) mice are selected, colon tissues are cultured in vitro, inflammatory response is induced by using interleukin-1beta (IL-1beta) stimulation, an age-related intestinal inflammation model is constructed, the inflammation phenotype of the mouse model and the activity of a TLR4 / MyD88 / NF-kappa B signaling pathway are determined by comparing WT and TLR4 <- / ->, and the age-related intestinal inflammation model is constructed. The core function of the TLR4 in regulating age-dependent intestinal barrier dysfunction is defined, a regulatory network of the TLR4 in intestinal immune aging and flora-host interaction is disclosed, and an age personalized medical strategy is promoted. According to the invention, an efficient and customizable multi-age in-vitro intestinal inflammation model is constructed, and an innovative platform is provided for mechanism analysis and intervention drug screening of TLR4 targeted therapy and age-related intestinal diseases.

The invention provides a crocodile source Aeromonas daka argH gene deletion strain, a crocodile source Aeromonas daka argH gene back-filling strain and a construction method and application of the crocodile source Aeromonas daka argH gene deletion strain and the crocodile source Aeromonas daka argH gene back-filling strain, an argH gene recombinant suicide plasmid pRE112-delta argH is constructed by adopting a seamless cloning method, an Aeromonas daka strain is jointed with E.coli WM3064 containing the pRE112-delta argH to obtain the argH gene deletion strain delta argH, and a corresponding gene back-filling strain C delta argH is further constructed. Through biological characteristic analysis, the argH gene deletion reduces synthesis of arginine of Aeromonas dakara and biofilm formation amount, reduces growth rate, proteolytic activity and resistance to crocodile-derived antibacterial peptide, but enhances hemolytic activity, and significantly improves the survival rate of Caenorhabditis elegans, so that the Aeromonas dakara has a good application prospect in treatment of caenorhabditis elegans, and the Aeromonas dakara has a good application prospect in treatment of caenorhabditis elegans. Important reference is provided for further researching the regulation effect of the argH gene in a bacterial pathogenic mechanism and developing related prevention and control strategies.

The invention discloses application of a yncJ gene in regulation and control of toxicity of salmonella typhimurium, and belongs to the technical field of biology. According to the invention, the function of the yncJ gene is studied by constructing the yncJ gene deleted strain of salmonella typhimurium, and it is found that after the yncJ gene is deleted, the multiplication capacity of the strain in macrophages is significantly reduced; infected mouse experiments show that the lethal ability of the yncJ gene deleted strain to mice is remarkably reduced. Moreover, the conventional biochemical characteristics and the in-vitro growth speed of the yncJ gene deletion strain are not obviously changed, so that the yncJ gene deletion strain has an application prospect in production of attenuated vaccines for preventing and treating salmonella typhimurium.

This document provides details regarding the construction of a recombinant African swine fever virus (ASFV) live attenuated vaccine for the prevention of African swine fever (ASF) caused by various viral strains of ASFV such as the highly virulent Georgia 2007 isolate (“ASFV-G”). Exemplary vaccines comprise an ASFV-GΔI177l modified virus, i.e., a recombinant ASFV-G modified by deletion of a portion of the I177L ORF such that the I177L gene is rendered non-functional.

The invention belongs to the technical field of genetic engineering, and particularly relates to a method for increasing the yield of lincomycin by knocking out a streptomyces lincomycin pAgene0296 gene. The method comprises the following steps: knocking out a coding gene pAgene0296 of TetR / AcrR family repressor protein in streptomyces lincomycin, and fermenting the obtained mutant strain to produce lincomycin. Experimental results show that after the pAgene0296 gene of the streptomyces lincomycin is knocked out, the yield of the lincomycin is remarkably increased. The invention further provides a construction method of the pAgene0296 gene deletion type streptomyces lincoli mutant strain, according to the method, the coding gene of the streptomyces lincoli is directionally modified through a gene engineering technology, so that the pAgene0296 gene is knocked out, and the obtained pAgene0296 gene deletion type streptomyces lincoli mutant strain can be used for efficiently producing lincomycin or a product in the synthesis process of the lincomycin.