266 results about "Gene Deletions" patented technology

The invention discloses a streptococcus suis type 2 gene deletion strain as well as a construction method and application thereof, and belongs to the technical field of biological products. According to the invention, the B9H0105210 gene in the genome of the streptococcus suis serotype 2 SC19 is deleted in a homologous recombination manner for the first time, the hydrogen peroxide and nitric oxide stress resistance of the constructed streptococcus suis serotype 2 gene deleted strain is reduced, and the pathogenicity to mice is obviously weakened; moreover, the growth performance of the streptococcus suis type 2 gene-deleted strain is not greatly different from that of a wild strain SC19, the condition of gene recovery does not exist after continuous passage, and the streptococcus suis type 2 gene-deleted strain has an application prospect as an attenuated live vaccine, has a good immune protection effect on different genotypes of streptococcus suis such as the streptococcus suis type 2 strain SC19 and streptococcus suis type 2 P1 / 7, and has a good application prospect. And a certain cross immune protection performance is achieved.

The invention relates to the technical field of gene engineering, and particularly discloses streptococcus agalactiae WC1535 delta cps delta hylB as well as a construction method and application thereof. The streptococcus agalactiae WC1535 [delta] cps [delta] hylB is preserved in Guangdong Microbial Culture Collection Center on February 28, 2025, the preservation number is GDMCC NO.65955, and the address of the preservation unit is the 5th building, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. Compared with a traditional single-fragment deletion method, the toxicity of the deletion strain is not prone to being enhanced, and compared with a traditional injection immune vaccine, the gene deletion strain is used for preparing the tilapia oral live vaccine, the immune operation is simpler, and more labor cost is saved.

Provided herein are diagnostic methods for identifying cancers suitable for treatment with an MTA-cooperative PRMT5 inhibitor, such as antibody-based methods and nucleic acid-based methods. The patients carry mutations that adequately sensitize cancer cells to an MTA-cooperative PRMT5 inhibitor, such as complete and certain partial MTAP gene deletions. Also provided are methods of treating the patients selected by such diagnostic methods.

The invention relates to the technical field of biology, in particular to application of glucoside hydrolase FsGH28c and a coding gene thereof in regulation of strain pathogenicity. The invention provides a glucoside hydrolase coding gene FsGH28c related to development, carbon source utilization and pathogenicity of fusarium solani, and after the FsGH28c gene is knocked out, the sporulation quantity is reduced, spore development is deformed, and the utilization rate of a carbon source (especially pectin) is reduced. In addition, the colonization amount of the FsGH28c gene deletion mutant in host pepper root fungi is reduced, and the pathogenicity is reduced. The FsGH28c gene can positively regulate the pathogenicity of fusarium solani, and can be used as an important candidate gene for research on the pathogenesis of fusarium solani.

The invention discloses a PDCoV JS2021-LX strain NS6 gene deletion recombinant virus and a construction method thereof, on the basis of PDCoV JS2021-LX strain infectious clone successfully constructed by a BAC system, the upstream and downstream of the NS6 gene are cut by adopting a CRISPR / Cas9 technology, then the original NS6 gene segment is replaced by green fluorescent protein EGFP through a homologous recombination mode, an NS6 gene deletion recombinant plasmid is obtained, and the NS6 gene deletion recombinant virus is constructed. And after the LLC-PK1 cell is transfected, the recombinant virus without the NS6 protein is rescued. The recombinant virus with the PDCoV NS6 gene deleted is successfully rescued, the virus replication level of the recombinant virus and the virus is rescued through in-vivo and in-vitro evaluation, and pig experiment results show that the pathogenicity of the recombinant virus is reduced, and the recombinant virus has the potential of serving as a candidate strain of an attenuated live vaccine.

Disclosed herein are methods, compositions, and devices for use in diagnosis and treatment of cancer. The methods include sequencing a panel of regions in cell-free nucleic acid molecules and detecting one or more biomarkers that are indicative of a cancer.

The invention relates to the technical field of viruses, in particular to a construction method and application of an African swine fever virus strain. According to the invention, the three virulence genes MGF110-9L, EP402R and I73R of the African swine fever virus are taken as targets for the first time to construct a virus strain with deletion of the three virulence genes, and the virus strain is found to be capable of effectively reducing the virus toxicity but retaining better immunogenicity, can improve the immunity of an organism to the African swine fever virus, and has a relatively great application prospect.

The present application relates to the field of veterinary diagnostic technology, and specifically to a probe composition, a primer composition, and a triple fluorescence PCR kit and related methods for differential diagnosis of three genes of different strains of African swine fever composed of the above-mentioned probe composition and primer composition. The kit includes primer probes specific to the B646L gene, the CD2v gene, and the I177L gene. The present application designs fluorescent PCR primers and probes for the B646L gene, the CD2v gene, and the I177L gene, respectively, and establishes a triple fluorescence PCR detection method, which can quickly identify African swine fever virus strains and gene-deficient strains. At the same time, it has no cross-reaction with swine fever virus, porcine reproductive and respiratory syndrome virus, pseudorabies virus, porcine epidemic diarrhea virus, porcine circovirus, porcine parvovirus, healthy whole blood, oropharyngeal swabs, nasal swabs, anal swabs, environmental swabs, pig tissues, etc., and has good specificity.

The invention relates to a dual ERA-LFD kit for visually and rapidly detecting salmonella typhimurium or a single-phase variant thereof, a primer group and application of the dual ERA-LFD kit and the primer group, and aims at solving the problems that an existing detection method is complex in operation, long in time consumption and depends on professional equipment, and primer cross interference, high false positive rate and incapability of distinguishing the single-phase variant caused by the fact that dual ERA primer design is not standard. Screening to obtain a specific primer probe combination aiming at the fljB gene deletion region and the STM4495 gene; a dual ERA-LFD detection system is constructed, and double targets are synchronously amplified under a constant temperature condition; visual interpretation is realized through a double-detection-line color development mode of the transverse flow test strip. The detection specificity can be remarkably improved, and false positive is effectively avoided; the accurate distinguishing between the salmonella typhimurium and the single-phase variants is realized; the detection process is rapid and simple, does not need professional instruments and personnel, and is suitable for on-site rapid screening; the detection cost is obviously reduced and the basic application feasibility is improved.

The invention provides a deletion strain and a supplementation strain of related genes of Aeromonas dakara PhoBR and QSS as well as a construction method and application of the deletion strain and the supplementation strain, a deltaahyR, deltaluxS and deltaqseB gene deletion strain and a supplementation strain as well as a deltaphoB deltaahyR, deltaphoB deltaluxS and deltaphoB deltaqseB double-gene deletion strain are successfully constructed, it is proved that the PhoBR can directly regulate and control the related genes ahyR, luxS and qseB of the QSS in the Aeromonas dakara, negative regulation and control on the ahyR and the luxS and positive regulation and control on the qseB are shown, the QSS related genes can be directly regulated and controlled in the Aeromonas The regulation and control are crucial to the survival and environment adaptation of bacteria. Besides, through a related bioinformatics method, possible binding domains of part of PhoB and three QSS related genes ahyR, luxS and qseB have been predicted, and a scientific basis is further provided for immune prevention and treatment of Aeromonas dakara.

The invention belongs to the technical field of biology and new medicine, and discloses a method for constructing a myxobacteria expression chassis with genetic stability and application of the myxobacteria expression chassis. Through gene deletion and overexpression analysis, it is verified that the spontaneous mutation rate of a strain can be reduced by deletion of a TLS pathway and overexpression of RecA, and a myxobacteria strain 2K7 with genetic stability is further constructed on the basis. The spontaneous mutation rate of the new strain is reduced by 11.5 times, and the transformation capability of growth plasmids of the strain is obviously improved. According to the research, the genetic stability of the myxobacteria is improved for the first time through a repair way after modification and replication, and the research method and the research result are innovative.

The invention relates to an establishment method and application of in-vitro intestinal inflammation models of mice of different ages, and relates to the technical field of biology. According to the invention, young, adult, middle-aged and old wild type (WT) and TLR4 gene deletion type (TLR4 <- / ->) mice are selected, colon tissues are cultured in vitro, inflammatory response is induced by using interleukin-1beta (IL-1beta) stimulation, an age-related intestinal inflammation model is constructed, the inflammation phenotype of the mouse model and the activity of a TLR4 / MyD88 / NF-kappa B signaling pathway are determined by comparing WT and TLR4 <- / ->, and the age-related intestinal inflammation model is constructed. The core function of the TLR4 in regulating age-dependent intestinal barrier dysfunction is defined, a regulatory network of the TLR4 in intestinal immune aging and flora-host interaction is disclosed, and an age personalized medical strategy is promoted. According to the invention, an efficient and customizable multi-age in-vitro intestinal inflammation model is constructed, and an innovative platform is provided for mechanism analysis and intervention drug screening of TLR4 targeted therapy and age-related intestinal diseases.

The invention provides a crocodile source Aeromonas daka argH gene deletion strain, a crocodile source Aeromonas daka argH gene back-filling strain and a construction method and application of the crocodile source Aeromonas daka argH gene deletion strain and the crocodile source Aeromonas daka argH gene back-filling strain, an argH gene recombinant suicide plasmid pRE112-delta argH is constructed by adopting a seamless cloning method, an Aeromonas daka strain is jointed with E.coli WM3064 containing the pRE112-delta argH to obtain the argH gene deletion strain delta argH, and a corresponding gene back-filling strain C delta argH is further constructed. Through biological characteristic analysis, the argH gene deletion reduces synthesis of arginine of Aeromonas dakara and biofilm formation amount, reduces growth rate, proteolytic activity and resistance to crocodile-derived antibacterial peptide, but enhances hemolytic activity, and significantly improves the survival rate of Caenorhabditis elegans, so that the Aeromonas dakara has a good application prospect in treatment of caenorhabditis elegans, and the Aeromonas dakara has a good application prospect in treatment of caenorhabditis elegans. Important reference is provided for further researching the regulation effect of the argH gene in a bacterial pathogenic mechanism and developing related prevention and control strategies.

This document provides details regarding the construction of a recombinant African swine fever virus (ASFV) live attenuated vaccine for the prevention of African swine fever (ASF) caused by various viral strains of ASFV such as the highly virulent Georgia 2007 isolate (“ASFV-G”). Exemplary vaccines comprise an ASFV-GΔI177l modified virus, i.e., a recombinant ASFV-G modified by deletion of a portion of the I177L ORF such that the I177L gene is rendered non-functional.

The invention provides a method for increasing the yield of indole-3-acetic acid through a streptomyces scabies SCAB31831 gene, which comprises the following steps: deleting the SCAB31831 gene in streptomyces scabies through a genetic engineering way to obtain an indole-3-acetic acid high-yield engineering strain, and fermenting the strain to produce the indole-3-acetic acid. Wherein the sequence of the SCAB31831 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the method in preparation of an indole-3-acetic acid high-yield microbial preparation, and provides an indole-3-acetic acid high-yield freeze-dried bacterial powder. According to the invention, the SCAB31831 gene negatively related to the biosynthesis of the indole-3-acetic acid is identified, and the gene is deleted in streptomyces scabies, so that the indole-3-acetic acid high-yield strain and freeze-dried bacterial powder based on the strain are obtained, and a technical support is provided for improving the fermentation yield of the indole-3-acetic acid in industrial production.

The invention discloses a construction method and application of a toxoplasma gondii polygene deletion vaccine strain. According to the invention, a TgBFD1 gene and a TgDAHPS gene are respectively knocked out from an ME49 [delta] 6pgdh1 insect strain through a gene editing technology, and a polygene deleted insect strain [delta] 6pgdh1 [delta] bfd1 [delta] dahps is successfully constructed; the strain can be subcultured in vitro, has the characteristics of no toxicity and no formation of tissue cysts in a host body, has better immune protection after immunization and inoculation, and can resist lethal infection of highly toxic strains. The toxoplasma gondii polygene deletion strain provided by the invention has better safety than ME49 delta 6pgdh1 strain, the toxicity of the toxoplasma gondii polygene deletion strain is obviously reduced, tissue cysts are not formed, the toxoplasma gondii polygene deletion strain can effectively resist infection of wild poisonous strains, the toxoplasma gondii polygene deletion strain has good immune protection capability, the defects that ME49 delta 6pgdh1 still has toxicity and can form tissue cysts are overcome, and the toxoplasma gondii polygene deletion strain has good application prospects. A method is provided for developing a safe and efficient toxoplasma gondii vaccine.

The invention discloses a toxoplasma gondii attenuated strain with deletion of a Tgsrs51 gene and application of the toxoplasma gondii attenuated strain, and belongs to the technical field of gene engineering. A Tgsrs51 gene is deleted in a genome of the toxoplasma gondii attenuated strain; and the ID (Identity) number of the Tgsrs51 gene on a ToxoDB (Database) website is TGME49308840. A Tgsrs51 gene deleted strain of a toxoplasma gondii type II Pru strain is constructed by utilizing a CRISPR-Cas9 technology, and experiments prove that the Tgsrs51 gene deletion can remarkably reduce the invasion efficiency of the toxoplasma gondii Pru strain, the secretion capacity of micronematosome MIC2 and the tachyzoite proliferation rate, lead to in-vitro growth limitation, remarkably improve the survival rate of infected mice and remarkably reduce the number of intracerebral cysts. The invention provides a theoretical basis for researching a molecular mechanism and an immune mechanism of toxoplasma gondii pathopoiesis, and provides a new strategy for prevention and control of toxoplasmosis.

A CVB virus expression vector comprising a PIV5 W3A viral genome comprising a mutation at amino acid residue S157 or S156 of the P / V gene and a deletion of the small hydrophobic (SH) gene of the PIV5 W3A viral genome, wherein the amino acid substitution at amino acid residue S157 or S156 comprises a substitution of serine (S) with phenylalanine (F) or asparagine (N), and the SH gene has a deletion of the SH open reading frame or the entire SH gene transcription unit. The CVB virus expression vector expresses a heterologous polypeptide, including SARS-CoV-2 spike (S), and / or nucleocapsid (N) and / or membrane (M) proteins, RSV fusion protein (F), or other antigens.

The invention relates to the technical field of biology, in particular to application of a rice OsGH17 gene in regulation and control of a rice root system. The regulation of the rice root system is to promote the development of the rice root system or inhibit the development of the rice root system. At present, no literature report about application of the rice OsGH17 gene to regulation and control of rice root systems is found. The invention shows that the OsGH17 gene deletion can promote the development of the rice root system, and the OsGH17 gene overexpression can inhibit the development of the rice root system. The regulation characteristic of the OsGH17 gene on the influence of the rice root system is defined, and an important reference is provided for deeply researching the function and action mechanism of the glucoside hydrolase in the plant in the rice.

The invention provides a toxoplasma gondii gene deletion strain with an immune protection effect and application of the toxoplasma gondii gene deletion strain, and belongs to the technical field of veterinary parasitology, veterinary immunology and parasite molecular biology. According to the toxoplasma gondii gene deletion strain, a gamma-glutamyl hydrolase gene is deleted. The invention also provides a method for constructing the insect strain. The method comprises the following steps: constructing a circular plasmid pSAG1-Cas9-U6-sgGGH; constructing a repair template containing upstream and downstream homologous arms of the gamma-glutamyl hydrolase gene and a DHFR resistance gene; co-transfecting the annular plasmid and the repair template to a toxoplasma gondii ME49 strain; performing drug screening on pyrimethamine to obtain positive monoclone; and confirming that the GGH gene is completely knocked out through PCR (Polymerase Chain Reaction) detection, sequencing analysis and immunofluorescence detection. The toxoplasma gondii gene deletion strain can provide a remarkable protection effect on toxoplasma gondii infection and is high in safety.

This invention discloses a candidate strain of *R. anatipestifer* double-gene deletion attenuated vaccine, its construction method, and its application. The candidate strain of *R. anatipestifer* double-gene deletion attenuated vaccine is *R. anatipestifer* strain CH-1. B739_RS01935-B739_RS07625 A candidate attenuated live vaccine strain with double gene deletion, deposited at the China Center for Type Culture Collection (CCTCC) on May 22, 2025, with accession number CCTCC NO: M20251080, at Wuhan University, Wuhan, China, has been deposited on that date. The attenuated live strain constructed in this invention showed no significant impact on the weight gain of ducklings after immunization, demonstrating good safety. Furthermore, the protection rate against challenge with the wild-type virulent RACH-1 strain reached 83.3% after immunization, indicating excellent immunoprotective efficacy.

The invention discloses application of a compound AZD5718 in preparation of a medicine for preventing and / or treating cholestatic liver diseases, and belongs to the field of biological medicines. The compound AZD5718 can effectively improve hepatocyte damage and bile metabolism disorder caused by Mdr2 gene deletion, provides solid experimental data support for developing novel drugs for treating cholestatic liver diseases, is expected to provide differentiated therapeutic drug choices for clinic, and has important medical value and social significance.

The invention discloses a method for distinguishing a vibrio alginolyticus wild type virulent strain and a cya gene deletion type low virulent strain based on proteomics. According to the method, a peak area ratio of ATP synthase subunit alpha (atpA) to phosphopentose mutase (deoB) in vibrio alginolyticus, which is measured by proteomics, is utilized to judge an adenylate cyclase coding gene cya-deleted attenuated strain and a wild virulent strain, the vibrio alginolyticus cya gene deleted attenuated strain with a result of atpA / deoB in a sample to be detected being less than 0.2, and the vibrio alginolyticus cya gene deleted attenuated strain with a result of atpA / deoB in the sample to be detected being less than 0.2 is a wild virulent strain with a result of atpA / deoB in the sample to be detected being less than 0.2. The vibrio alginolyticus strain with the result of atpA / deoB being 1.2 or above is a vibrio alginolyticus wild type virulent strain. The distinguishing method is easy to operate, high in detection speed and reliable in result.

Contemplated treatments and methods produce substantially increased quantities of memory T-cells and a persistent immune response by subcutaneous and / or subdermal co-administration of (1) a vector comprising a recombinant nucleic acid that encodes a cancer associated epitope, a cancer specific epitope, and / or a neoepitope, (2) an immune stimulating cytokine, and (3) a checkpoint inhibitor. Most typically, the co-administration is performed at substantially the same location, preferably within 1-21 days from each other, and the vector is an adenoviral expression vector, for example, included in a viral particle such as an AdV5 virus with a deletion of the E2b gene.

The invention discloses an ADO (adenosine dioxygenase) gene deleted zebrafish model as well as a construction method and application thereof. According to the invention, ADOa gene knockout is carried out on a model organism (zebrafish) to successfully construct an ADO gene deleted zebrafish model. Based on the model, the function of the ADO gene in fish taurine metabolism can be researched more deeply, so that a new scientific basis is provided for understanding the ADO function of zebra fish and even other fishes, and meanwhile, the model can be used for drug screening and provides a theoretical basis and technical support for breeding and genetic improvement of aquaculture industry.

The invention provides a genetic engineering strain and a construction method thereof, the strain is a target gene deleted strain obtained by knocking out a target gene in a rod-shaped bacterial strain, and the target gene is one of minC and minD genes or a gene combination thereof. According to the present invention, the minC and / or minD gene deletion strain is obtained by knocking out the minC and / or minD gene in the rod-shaped bacterial strain, such that cell division abnormality can be caused, chromosome-free small cells are generated, and the small cells have characteristics of biological activity, no chromosome DNA, and no growth or division propagation. The small cells can generate smaller body immune response, and meanwhile, the LPS modified by the small cells can generate smaller cytotoxic reaction, so that side effects can be greatly reduced when the small cells are applied to human or animal bodies.

Methods are provided for detecting gene deletions and driver mutations using tumor-specific methylation patterns from cell-free DNA and tissue biopsies. The methods exploit the mutual exclusivity between tumor-specific methylation and homozygous gene deletion, enabling detection through absence of expected methylation signals. Applications include detection of MTAP, PTEN, and RB1 deletions, as well as prediction of EGFR single nucleotide (SNV) and small (up to 50 base pair) insertions and deletions (indel) driver mutations, enabling identification of patients eligible for targeted therapies.

The invention relates to the technical field of biological medicines, and discloses a construction method and expression application of an Hcp gene deleted strain of a rahnella aqua KCL-5 strain for fish. The Hcp 0186 gene deletion strain is constructed by using a homologous recombination method, the comparison between the deletion strain and a wild strain is carried out, and the result shows that the deletion of the Hcp 0186 gene has an influence on the growth speed, the pathogenicity of the Hcp 0186 gene to the zebra fish is obviously reduced, the survival rate of the infected zebra fish is increased from 0% to 52%, and the Hcp 0186 gene deletion strain can be used as a fish attenuated live vaccine. A recombinant prokaryotic expression vector pET32a-Hcp and expression and purification of fusion protein of the recombinant prokaryotic expression vector pET32a-Hcp are further constructed, and Hcp 0186 rabbit polyclonal antibody serum is prepared as a primary antibody and can be applied to localization expression analysis of fish tissues infected by the bacterium and detection of WB and IHC. Results prove that the Hcp 0186 gene deletion of the strain provides an attenuated strain vaccine and clinical diagnosis and detection application of infected fish of the attenuated strain vaccine, and a scientific basis can be provided for revealing a molecular pathogenic mechanism of the strain and development and application of a mutant strain of the strain.

The invention discloses a quadruple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer probe combination for detecting African swine fever virus and application. The primer probe combination comprises a first primer probe combination, a second primer probe combination, a third primer probe combination and a fourth first primer probe combination, the primer probe is capable of specifically targeting ASFV p72 gene, CD2v-I gene, CD2v-II gene and I177L gene, and the detection kit developed based on the primer probe combination can rapidly diagnose African swine fever virus and identify CD2v gene I and II type and CD2v and I177L gene deleted strains. The kit has extremely high sensitivity, the lowest detection limit can reach 8 copies / mu L, it is ensured that trace virus DNA can be detected, and missing detection is avoided.