The invention relates to the field of
biology, and particularly provides a 293H
cell line for expressing an influenza
virus PB1
gene, the
cell line is classified and named as an HEK-293H
cell line, and the preservation number is GDMCC NO: 66761. The preservation date is July 25, 2025, and the strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation address is the 5th floor of the Experimental Building, No. 100 Courtyard, Middle Xianlie Road, Guangzhou District, Guangdong Province. In addition, the invention also provides an
MDCK cell line for expressing the influenza
virus PB1
gene, both the
MDCK cell line and the
MDCK cell line can express PB1
protein, and rescue and propagation of the PB1
gene knockout (PB1-KO) replication defective influenza
virus can be realized. Meanwhile, the invention also discloses application of the two cell lines.