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Gene Specific primers enhance sensitivity by directing all of the RT activity to a specific message instead of transcribing everything in the mix. If you are performing a one-step RT-PCR, gene specific primers are used because the RT primer is also your reverse primer for the PCR step.
A dual-gene-specific primer set for rapid identification of chiropteran species and a method for applying the same
This invention discloses a dual-gene specific primer set for rapid identification of Chiroptera species and its application method. The primer set includes primer pair COI-F / COI-R for amplifying the mitochondrial COI gene and primer pair Cytb-F / Cytb-R for amplifying the Cytb gene, with nucleotide sequences shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, and SEQ ID NO.4, respectively. Experiments have demonstrated that this primer set exhibits good interspecific specificity and intraspecific conservation, effectively distinguishing Chiroptera from other closely related mammals and accurately identifying cryptic species and juvenile samples that are difficult to distinguish morphologically. This invention employs dual-gene markers for mutual verification, significantly improving the accuracy and reliability of identification, providing a powerful molecular biology tool for rapid and accurate identification of Chiroptera species, and has significant application value in fields such as biodiversity surveys, disease tracing, and ecological conservation.
Owner:HENAN NORMAL UNIV