196 results about "Gene specific primer" patented technology

A method for zygosity analysis of the maize Cry1F event TC1507 is provided. The method provides TC1507 event-specific and maize endogenous reference gene-specific primers and TaqMan probe combinations for use in an endpoint biplex TaqMan PCR assay capable of producing robust genotype calls for assisting in molecular breeding of TC1507.

The invention provides a primer group for simultaneously identifying a wild strain and a gene deletion strain of African swine fever based on a multiple qPCR technology and a test kit, and belongs tothe technical field of virus detection. The primer probe group for simultaneously identifying the wild strain and the gene deletion strain of the African swine fever based on the multiple qPCR technology comprises a p72 gene specific primer probe, a CD2v gene specific primer probe and an MGF gene specific primer probe. The test kit comprises the primer probe group. Multiple qPCR detection is performed by adopting the test kit; p72 gene specific primers of a conserved gene of ASFV can amplify the wild strain and the gene deletion vaccine strain; specific primers of a CD2v gene and an MGF gene can only amplify the wild strain; and the three pairs of primers are combined for use, so that the wild strain and the gene deletion vaccine strain of the ASFV can be simultaneously identified. The primer group and the test kit have the advantages of accurate detection, sensitivity, high efficiency, low cost and the like, and have relatively high practical value for diagnosis of clinical samples and the breeding industry.

This invention relates to a body fluids identification method and kit. A parallel, multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay for the definitive identification of body fluids commonly encountered in forensic casework analysis, namely blood, saliva, semen, and vaginal secretions. The methodology is based on gene expression profiling analysis in which the body fluid-specific genes are identified by detecting the presence of appropriate messenger RNA species. Gene-specific primers are labeled with fluorescent dyes, separated and subjected to laser induced fluorescence for identification of body fluid-specific genes present in a sample stain.

The invention relates to a method for quantitating ammonia oxidizing bacteria in a sediment of an aquiculture environment by a real-time fluorescent quantitative PCR (polymerase chain reaction) technology, which belongs to the field of environmental microbial ecology. The method comprises the steps that DNA (deoxyribonucleic acid) of a sample sediment is extracted and quantitated; an HZO (hydrazine oxidordeuctase) gene specific primer is subjected to PCR amplification; standard plasmids are constructed; and a fluorescent quantitation PCR amplification curve and a standard curve are made. The quantity of the anaerobic ammonia oxidizing bacteria in a sample is calculated by comparing a detected sample Ct value with the standard curve; an obtained log value of the initial template gene copy number has a better linear relationship with the Ct value; and a regression coefficient is greater than 0.99. According to the method, an HZO gene is used for conducting quantitative analysis on the ammonia oxidizing bacteria under an anaerobic condition; the deficiencies that the specificity is not high, the coverage ratio is low and the method is inaccurate due to the fact that the a 16S rRNA (ribosomal ribonucleic acid) gene is used for quantitating in the existing method are overcome; and the method for quantitating the ammonia oxidizing bacteria in the sediment of the aquiculture environment is high in specificity, quick and accurate.

Methods of preparing a plurality of sample-barcoded anchor-domain-flanked gene specific deoxyribonucleic acid (DNA) fragments from a template nucleic acid, e.g., ribonucleic acid (RNA), sample are provided. Aspects of the methods include employing a set of gene specific primer pairs, wherein each pair of gene specific primers is made up of a forward primer and a reverse primer, at least one of which includes a sample barcode domain. The methods find use in a variety of different applications, including high-throughput sequencing, e.g., expression profiling, applications, including of small biological samples, e.g., single-cells.

The invention discloses a KASP marker typing primer combination for high-throughput detection of mutation sites of an AhFAD2B gene and an application of the primer combination, belongs to the technical field of molecular genetic breeding and relates to a special primer combination for detecting types of the AhFAD2B gene of high-oleic-acid peanuts by a high-throughput SNP molecular marker and the application of the primer combination. The primer combination is the KASP marker typing primer combination for detecting C>T mutant sites at 814 sites of the AhFAD2B gene of high-oleic-acid peanuts andcomprises an A-labeled wild type AhFAD2B gene specific primer with a nucleotide sequence shown in SEQ ID No.1, a B-labeled mutant AhFAD2B gene specific primer with a nucleotide sequence shown in SEQID No.2 as well as a AhFAD2B gene general primer with a nucleotide sequence shown in SEQ ID No.3. The high-throughput KASP molecular marker gene typing specific primers are designed for novel mutant 814 C>T allelic variation of the AhFAD2B of high-oleic-acid peanuts, novel mutants of the AhFAD2B can be effectively identified, and the problem that identifying methods in the prior art can only identify 448 G>A allelic variation of classic F435 mutant sites of the AhFAD2B is solved.

An analytical method for quantitative gene expression amount determination and comparison has great significance for disease-related gene screening and in clinical early diagnosis and development of specific medicine. The present invention utilizes quantitative characteristic of biological luminance process is sequencing to compare the gene expression amount of the same gene in different individuals or samples and to search disease-related gene. The specific steps includes: making mRNA with different sources by using a proper method and mixing in equal amount to form PCR substrate; PCR amplification with the primer corresponding to the gene of different sources and gene specific primer; and determining sequence by using bioluminescence analysis process with base variety to replace gene of different source.

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is T. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of T, C or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.