50 results about "Transcriptional expression" patented technology

This invention relates to the fields of molecular biology and biomedicine, and discloses siRNA for the expression of the *Schistosoma japonicum* Hsc20 gene and its applications. The siRNA contains a nucleotide sequence that hybridizes to a target sequence of the *Schistosoma japonicum* Hsc20 gene, wherein the target sequence is selected from the sequences shown in SEQ ID NO. 1-SEQ ID NO. 4. The siRNA specifically inhibiting the expression of the *Schistosoma japonicum* Hsc20 gene provided by this invention can be used to interfere with the transcription and expression of the *Schistosoma japonicum* Hsc20 gene and the growth and development of *Schistosoma japonicum*. In vitro experiments have confirmed that the siRNA provided by this invention can efficiently silence the *SjHsc20* gene; and in vivo RNA interference experiments in mice show that this siRNA can induce 50.62% (…) P The insect reduction rate was <0.05% and 44.29% ( P A liver oocyte reduction rate of <0.01% is suitable for preparing drugs to treat schistosomiasis.

The invention belongs to the technical field of medicines, and particularly relates to application of pinosine and polymyxin in preparation of antibacterial drugs, and the antibacterial drugs aim at one or more of escherichia coli, salmonella and klebsiella pneumoniae. The antibacterial agent aims at bacteria which are resistant to polymyxin. The bacteria directed by the antibacterial agent are multi-drug-resistant gram-negative bacteria. A checkerboard method minimum inhibitory concentration test and an in-vitro time sterilization curve prove that the pinosine effectively recovers the sensitivity of bacteria to the polymyxin, and meanwhile, a staining experiment and fluorescent quantitative PCR prove that the pinosine can effectively recover the sensitivity of the bacteria to the polymyxin by destroying the completeness of a bacterial cell membrane and inhibiting the transcription expression of a polymyxin drug-resistant gene mcr-1. The antibacterial activity of the polymyxin on bacteria is effectively enhanced. The invention provides a novel application of densipine in enhancing the antibacterial activity of polymyxin antibiotics, and can solve the technical problems of low clinical drug resistance, low therapeutic index and the like of polymyxin.

The invention provides a recombinant TKC vector applied to CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated 9) and a construction method of a mutant plant, and belongs to the technical field of gene editing. The invention provides a recombinant TKC vector applied to CRISPR-Cas9, the recombinant TKC vector comprises a U6 transcription box and / or a U3 transcription box, a target gene in the transcription box is located between a promoter and a terminator, and transcription expression of the target gene can be effectively guaranteed. By using the recombinant TKC vector and knocking out the EMF1 gene in a japonica rice dry-hui 89 material, the daily flowering time of the japonica rice dry-hui 89 material is obviously earlier, the flowering time of hybrid seed production of indica rice and japonica rice is encountered, the yield of hybrid seed production of indica rice and japonica rice is favorably improved, and the seed production cost is reduced. By utilizing the gene editing method provided by the invention, the daily flowering time of the japonica rice variety, i.e., the Yanhui 89 can be obviously advanced, and the problem of infertility during flowering during indica-japonica hybrid seed production is solved.

The present application relates to the field of agricultural biotechnology, in particular to a bidirectional promoter derived from Myceliopthora thermophila and application thereof. The bidirectional promoter derived from Myceliopthora thermophila has a unique bidirectional transcription activity feature, and can simultaneously and efficiently initiate the transcription and expression of two independent genes in the same regulation region in opposite directions. The present application provides a more favorable promoter element for the metabolic engineering of the industrial filamentous fungus Myceliopthora thermophila, significantly simplifies the construction process of a multi-gene co-expression system, and realizes the synergistic expression of two functional genes through a single promoter element, which has a wide application space and market prospect in the production of enzyme preparations, biological medicine products and the like.

The invention relates to the technical field of agricultural biology, in particular to a bidirectional promoter derived from thermophilic fungus destructor thermophilus and application of the bidirectional promoter. The bidirectional promoter derived from thermophilic fungus destructor thermophilus has unique bidirectional transcriptional activity characteristics, and can simultaneously and efficiently start transcriptional expression of two independent genes in opposite directions in the same regulatory region. A more favorable promoter element is provided for metabolic engineering modification of industrial filamentous fungus myceliophthora thermophila, the construction process of a multi-gene co-expression system is remarkably simplified, and synergistic expression of two functional genes can be realized through a single promoter element; the method has wide application space and market prospect in production of enzyme preparations, biological medicine products and the like.

This invention discloses the application of a branched-chain amino acid (BCAA) restricted diet in the preparation of drugs for treating liver metastases of tumors. Through mouse model experiments, this invention found that restricting the intake of BCAAs (valine, isoleucine, and leucine) in the diet, or using the SCD1 inhibitor A939572, can significantly inhibit liver metastases of tumors, especially pancreatic cancer liver metastases. Experimental results show that a BCAA restricted diet or SCD1 inhibitor can reduce the number and volume of liver metastases and inhibit their growth. This invention also reveals its mechanism of action: BCAA restriction reduces the propionylation modification of SREBP1, downregulates the transcriptional expression of its target gene SCD1, and thus reduces lipid synthesis, thereby exerting an anti-tumor liver metastasis effect. This invention provides a novel dietary intervention strategy and drug target for the treatment of liver metastases of tumors.

The invention provides a brain glioma immune microenvironment complexity assessment method and application, and the method comprises the following steps: obtaining m glioma samples, and carrying out transcriptome determination on each cell in each glioma sample to obtain transcriptional expression profile data of the cells; carrying out clustering analysis on transcriptional expression profile data of the single cells through K-means clustering, and preliminarily identifying lymphocytes and myeloid cells according to immune cell classical markers; carrying out clustering analysis on the identified transcription expression profile data of the lymphocytes and myeloid cells, and identifying n cell subgroups; calculating the enrichment score of the characteristic gene set of each cell subset in the glioma transcriptome sample, wherein the higher the score is, the higher the enrichment proportion of the cell subset in the sample is; and evaluating the complexity of immune cell enrichment in the glioma sample based on the enrichment score of the characteristic gene set. The immune cell infiltration complexity can be predicted, and the relationship between immune cell enrichment complexity and brain glioma prognosis can be identified.

The invention relates to a method for inferring a silkworm chrysalis stage development process based on trimethyl lysine hydroxylase. The method comprises the following steps: (1) constructing a correlation model of relative transcription expression quantity of a trimethyl lysine hydroxylase gene and a reference gene of a silkworm chrysalis sample of a variety to be detected and pupal stage development time; and (2) determining the relative transcription expression quantity of the trimethyl lysine hydroxylase and the reference gene in the silkworm chrysalis to be detected, and calculating the pupal stage development time according to the correlation model obtained in the step (1). According to the method, the development process of one or more silkworm chrysalis can be accurately measured, errors caused by traditional visual inspection can be reduced, a new detection method is provided for deducing the pupal development process of the silkworms, and an important basis is provided for judging moth formation time in silkworm seed production work.

The invention discloses an application of a PHD3 inhibitor in prevention and treatment of AIDS (acquired immune deficiency syndrome). Through multidisciplinary research means of immunology, biochemistry and the like, the situation that virus antisense protein ASP inhibits generation of I-type IFN in the HIV-1 infection process is analyzed, immune escape is achieved, and a latent infection mechanism is established. In the aspect of mechanism, the ASP can play the inhibiting role after transcriptional expression into protein in a body cell through post-translational modification, the 47-site proline of the ASP can be helped to realize the capability of inhibiting the immune response of the body after hydroxylation modification, and the post-translational modification is realized by depending on proline hydroxylase 3 (PHD3) of a host. On the basis of mechanism research, it is found that a PHD3 inhibitor Molidustat counteracts hydroxylation of PHD3 to ASP, so that immune escape of HIV-1 is blocked, infection is antagonized, and good development prospects of AIDS treatment and prevention drugs are shown.

The present application provides a low-oxygen-induced promoter for yeast cells and application thereof. The promoter of the present application can achieve appropriate driving of transcription / expression of a target gene under different oxygen environments, and plays a role of driving transcription / expression with medium and high intensity.

The invention discloses a kit for early diagnosis of young acute coronary syndrome based on plasma transcriptomics and application of the kit. The invention provides a computer device which comprises a processor and a computer program, and the processor executes the computer program to realize the following steps: S1) data receiving: receiving expression quantities of twelve proteins in a plasma sample of a patient to be detected; s2) data processing: substituting the expression quantities of the twelve proteins into a prognosis risk prediction model, and calculating to obtain a risk score value of a to-be-detected patient as a high-risk young coronary heart disease patient; and S3) data output: analyzing the possibility that the to-be-detected patient is a high-risk young coronary heart disease patient according to the risk score value. According to the early diagnosis kit and device for the young acute coronary syndrome, the acute coronary syndrome of a patient can be rapidly judged by detecting the transcriptional expression quantity of twelve proteins in peripheral venous blood plasma of the young patient, non-invasive detection and diagnosis are rapid and accurate, operation is easy, and repeatability is good.

The invention belongs to the field of gene engineering, and particularly relates to application and a method of an NtZFP26 gene in promoting nicotine synthesis. The invention finds and verifies that the tobacco C2H2 type zinc finger protein transcription factor NtZFP26 can be specifically combined with a promoter of a nicotine synthesis key gene NtQPT2, and activates the transcription expression of the nicotine synthesis key gene NtQPT2, thereby positively regulating the biosynthesis of nicotine. Experiments prove that the NtZFP26 gene specifically expressed at the root of tobacco is regulated by topping and hormones such as NAA, ABA and MeJA; knockout of the NtZFP26 gene can lead to significant reduction of tobacco nicotine content and synthesis key gene expression quantity. The invention provides a key gene target and a new technical approach for directionally regulating and controlling the nicotine content of tobacco through a genetic engineering means.

The present invention belongs to the field of biopharmaceutical technology and specifically discloses the use of ribose-5-phosphate in the preparation of a drug for treating inflammatory diseases, particularly osteoarthritis. The present invention demonstrates that ribose-5-phosphate can significantly increase SW1353 cell viability, inhibit the transcriptional expression of the inflammatory factors IL-6 and IL-8 in SW1353 cells, inhibit the protein expression of the inflammatory factors IL-6 and IL-8 in SW1353 cells, and inhibit the transcription and protein expression of matrix metalloproteinase 13 in SW1353 cells, thereby treating osteoarthritis. Therefore, ribose-5-phosphate has the potential to be used in the preparation of anti-inflammatory drugs.

The invention discloses an application of a rice OsPHR2 gene in regulation and control of selenium bioaugmentation. The rice OsPHR2 gene is shown as SEQ ID No: 1 in a sequence table, and the amino acid sequence of the protein of the rice OsPHR2 gene is shown as SEQ ID No: 2. According to the invention, the promoter of the rice OsPHR2 is adopted to drive the rice OsPHR2 to increase one to two copy numbers in the rice body, so that the content of phosphorus in the rice body is increased without influencing the growth, development and yield of the rice by means of moderately increasing the transcription expression of the rice OsPHR2, meanwhile, the absorption and accumulation of selenium can be promoted, and the biological enrichment enhancement of rice selenium is realized.

The invention discloses a core promoter for construction of a gene expression system in mammalian cells and application of the core promoter, and provides polynucleotide with promoter activity, a transcription expression cassette containing the polynucleotide, a recombinant expression vector, a recombinant host cell and application in an expression system. The core promoter provided by the invention can be combined with various enhancers and coding sequences in a modular manner, is suitable for constructing a drug-induced expression system, a tumor specific expression system, a synthesis loop and a gene expression regulation system related to cell therapy, and has good universality and application prospects.

The application discloses a kind of preeclampsia (Preeclampsia, PE) diagnosis and treatment biology marker and its application;The application is from clinical sample, cell and in-depth demonstration of overexpressed FBP2 at the level of body, which causes the mechanism of PE occurrence by inhibiting the invasion of trophoblasts, and the application verifies that the classic gene HEY1 downstream of NOTCH3 can be combined with the promoter region of FBP2 and transcribe to inhibit the expression of FBP2, FBP2 is combined with c-MYC protein and inhibits the transcriptional activity of c-MYC, down-regulates the transcriptional expression of TFAM, causes mitochondrial dysfunction, weakens the migration and invasion ability of trophoblasts, participates in the mechanism of PE occurrence, is a new mechanism clue obtained according to the results of pre-experiment and information analysis, is a new target for in-depth exploration of PE clinical diagnosis and treatment, and the research results can provide theoretical basis for guiding early prediction and intervention of PE in the future.

Disclosed in the present invention is a use of a 2-aminothiazole derivative in the preparation of an anti-aging drug. The 2-aminothiazole derivative can effectively alleviate microglial cell aging in vitro, reduce accumulation of senescence-associated β-galactosidase in aged cells, decrease secretion of a senescence-associated secretory phenotype, and ameliorate apoptosis and oxidative stress damage in the aged cells. In a mouse in vivo experiment, compounds A1-A10 can significantly improve aging-induced cognitive dysfunction and impairment of long-term and short-term learning and memory abilities, reduce transcriptional expression levels of pro-inflammatory factors in the brain, and alleviate neuroinflammation. In summary, the present inventors propose that 2-aminothiazole derivatives have good anti-aging efficacy and possess great application potential in anti-aging.

The application belongs to the field of medicine, and particularly relates to a synergistically treating chordoma combined drug and application thereof. The combined drug is Notch blocker PF03084014 and ELT or Notch blocker PF03084014 and DOX. Research proves that the Notch blocker PF0308401 targets and regulates the transcription expression of a key transcription regulatory factor Brachyury of chordoma, and inhibits tumor cell proliferation; and can obviously improve the drug resistance of chordoma to the chemotherapeutic drugs DOX and ELT. The application can provide an advantageous laboratory research basis and a transformation research direction for a chordoma drug treatment scheme.

This invention discloses the application of the HbCBF1 gene in regulating ethylene content in rubber trees, belonging to the field of agricultural biotechnology. By overexpressing the HbCBF1 gene (nucleotide sequence shown in SEQ ID NO.1), this invention significantly enhances the biosynthesis of ethylene in transgenic plants, manifested as an increased ethylene release rate, elevated content of the ethylene precursor ACC, and enhanced activity of key enzymes in the ethylene synthesis pathway, SAMS, ACS, and ACO. It also exhibits dwarfing phenotypes associated with excessive ethylene accumulation, including significantly reduced plant height, thinner stems, shorter internodes, and accelerated leaf senescence. Molecular mechanism studies show that HbCBF1 directly activates the transcriptional expression of SAMS1 by binding to the CRT / DRE cis-acting element in the SAMS1 gene promoter, thereby upregulating ethylene biosynthesis. This invention provides a new target gene and regulatory method for the genetic engineering improvement of rubber trees.

The invention discloses an ostrinia furnacalis nuclear receptor HR39 gene, an RNA interference fragment, dsRNA and application thereof, and relates to the field of agricultural pest control. The full-length cDNA sequence of the HR39 gene is as shown in SED ID NO: 1, the amino acid sequence of the coded protein is as shown in SED ID NO: 2, and the sequence of the RNA interference fragment is as shown in SED ID NO: 3. The full-length cDNA sequence of the HR39 gene is obtained, a dsRNA segment targeting the gene is designed, transcription expression of the HR39 gene is successfully interfered, and results show that ovary development of female individuals interfering the HR39 is remarkably inhibited, the egg laying amount and the egg hatching rate are remarkably reduced, and the reproductive capacity of the female individuals is remarkably inhibited. The HR39 gene provided by the invention provides a molecular target for designing a specific and efficient nucleic acid pesticide to inhibit reproduction of pests, and has a good application prospect in green prevention and control of ostrinia furnacalis.

This present disclosure provides compositions and methods of increasing activating transcription 4 (ATF4) expression in a subject, thereby preventing and / or treating cardiomyopathy-related diseases in the subject.

This invention discloses a graph reasoning and analysis system for addressing defects in cellular antigen processing, belonging to the field of knowledge graph reasoning technology, to solve the problem of slow interpretable localization of defect presentation. This invention constructs a detection likelihood set by integrating HLA typing, somatic cell variation, transcriptomic expression, immunopeptidomics, and surface HLA detection. It assembles direct and cross-presentation subgraphs using pattern gating and loading complex integrity rules, generating reachable paths and observation consistency matrices. It distinguishes between structural contradictions and detection limitations, performs node pruning and link contraction based on constraint propagation to form an individualized graph, and outputs defective nodes and paths based on minimum interpretable paths and causal graph cost search. It generates validation and intervention lists and clinical decision support outputs, thereby reducing unnecessary detection and intervention attempts and improving the timeliness and consistency of case analysis and strategy formulation.

This invention belongs to the field of biotechnology and discloses a high-yield rAAV plasmid system and its applications. The high-yield rAAV plasmid system of this invention includes a transgenic plasmid containing two terminal inverted repeat sequences, a packaging plasmid, a helper plasmid, and an shRNA expression cassette; the shRNA expression cassette includes a sequence capable of targeting the target gene in the transgenic plasmid. In the rAAV production process, the plasmid system of this invention inhibits the transcriptional expression of the target gene by expressing shRNA to target the coding region or target sequence of the target gene on the transgenic plasmid. The carefully designed target sequence, unlike coding regions of human and mammalian genes, can be broadly used in various transgenic plasmids, thereby increasing rAAV yield and significantly improving the rAAV production efficiency of mammalian cells, achieving the technical effects of improved rAAV druggability and reduced production costs.

The present application relates to the technical field of biological medicine, and provides a Chinese herbal medicine vesicle-LNP hybrid tumor mRNA vaccine and application thereof.The tumor mRNA vaccine is obtained by hybridizing Chinese herbal medicine nano vesicles and lipid nanoparticles loaded with tumor antigen mRNA.The present application reduces the safety risk of cationic lipid carriers by using the good biocompatibility of Chinese herbal medicine.Combined with the characteristics of Chinese herbal medicine, the nano vesicles are extracted, which promotes the LNP to more effectively target antigen presenting cells, promotes the uptake level of antigen presenting cells, enhances the phagocytosis of mRNA, and can enhance the targeting and transcription expression level of mRNA.Finally, the tumor mRNA vaccine hybridized by Chinese herbal medicine nano vesicles and LNP can strengthen the effect of inhibiting tumor progression, inhibiting tumor recurrence and inhibiting tumor metastasis after vaccination, and opens up a path for its combination with more abundant immunotherapy methods.

A method and device for predicting gene interaction relationships based on a graph neural network, the method comprising: preprocessing the transcriptional expression data of each experimental group, including standardizing the gene ID, and representing the gene transcriptional expression data of different experimental groups with a unified gene ID; obtaining raw data for subsequent analysis; calculating gene similarity according to each sample group, and obtaining similarity data between genes in the sample group; selecting gene pairs that meet set conditions as the basis for constructing a graph data structure by statistically analyzing and screening the data of all sample groups; further processing the raw data to ensure that all sample groups contain only genes defined in the graph structure; then, standardizing the data of these sample groups so that the data dimensions of all sample groups are consistent; and performing model training on the generated graph data group to ultimately obtain edge weight data in the graph structure, which can accurately reflect the interaction relationship between genes. The present invention can be used to analyze gene expression data, predict the interaction relationship between genes, and perform unsupervised learning in the absence of labeled data.

The present invention provides the use of rice OsERF103 protein and its encoding gene in improving plant drought tolerance, relating to the technical field of plant genetic engineering. The amino acid sequence of rice OsERF103 protein is shown in SEQ ID No. 2. The present invention clarifies for the first time that rice OsERF103 gene is induced to express by drought, and its transcriptional expression level gradually increases with the process of drought treatment and gradually decreases with the process of rewatering; drought tolerance analysis shows that OsERF103 overexpressing rice plants have a drought-resistant phenotype and a high survival rate, while Oserf103 mutants all show a drought-sensitive phenotype and a survival rate significantly lower than that of the wild type, indicating that OsERF103 is a positive regulatory factor in rice's response to drought. Rice OsERF103 protein and its encoding gene can be used to improve plant drought tolerance, which is of great significance for the study of the molecular mechanism of drought stress and the breeding of new drought-resistant varieties.

The invention discloses application of ammonium ferric citrate in preparation of a medicine for resisting grouper iridovirus. The effective concentration of the ammonium ferric citrate at the cellular level is 0.04-0.08 mM, and the effective concentration of the ammonium ferric citrate in vivo is 20 mg / kg. The in-vivo and in-vitro treatment of the ferric ammonium citrate can inhibit the replication of the SGIV. The ferric ammonium citrate with a safe concentration in in-vitro cells can significantly reduce cytopathy induced by SGIV infection and reduce viral protein expression and yield of progeny viruses, intraperitoneal injection of ferric ammonium citrate can inhibit transcription expression of viral genes in vivo, reduce the death rate of groupers caused by SGIV infection, and improve the safety of the groupers. The ferric ammonium citrate has a remarkable antiviral effect and has the potential of researching and developing the anti-iridovirus medicine. In addition, the ferric ammonium citrate can improve the oxidation resistance of cells or tissues, so that tissue pathological damage caused by virus infection is improved. As an antiviral drug, the ammonium ferric citrate has a certain application value in prevention and control of grouper iridovirus diseases.

The present invention belongs to the field of molecular biology, and particularly relates to the application of enhanced activity of NADP-ferredoxin reductase in improving the yield and conversion rate of L-glutamic acid. The enhanced activity is achieved by enhancing the transcriptional expression of NADP-ferredoxin reductase, or by mutating the NADP-ferredoxin reductase of the sequence shown in SEQ ID NO: 3, or by mutating the NADP-ferredoxin reductase of the sequence shown in SEQ ID NO: 3 and enhancing the transcriptional expression. The enhanced activity of the NADP-ferredoxin reductase of the present invention can reduce the production cost of glutamic acid, provide a new strategy for large-scale production, and has great application value.

This present disclosure provides compositions and methods of increasing activating transcription 4 (ATF4) expression in a subject, thereby preventing and / or treating cardiomyopathy-related diseases in the subject.

The invention discloses a cattle ACSS1 gene promoter as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the cattle ACSS1 gene promoter disclosed by the invention is any one sequence of ACSSI1-A-ACSS1-F as shown in SEQ ID NO.1-6. The invention further discloses a preparation method of the cattle ACSS1 gene promoter. Experiments find that the promoters ACSS1-B, ACSS1-C, ACSS1-D and ACSS1-E have obvious promoter activity in human mammary epithelial cells MAC-T, which is of great significance for revealing a regulatory mechanism of bovine ACSS1 transcriptional expression, verifying a high-quality beef cattle breeding scheme of a targeted bovine ACSS1 expression level and developing a new eukaryotic protein expression system. And the method can also be used for cell level screening of drugs for targeting bovine ACSS1 expression.