Novel resistant gene of chloromycetin and application of novel resistant gene of chloromycetin

Abstract

The invention provides a novel resistant gene of chloromycetin and application of the novel resistant gene of chloromycetin. The resistant gene has a sequence shown in SEQ ID No. 1 and is called aftera gene GMC; after tertiary sequencing of a genome, a drug-resistant gene known at present is not discovered on the genome, and then, through sequencing of RNA, it is discovered that GMC-encoded oxidoreductase has a very high transcription expression level in the presence of antibiotics. According to the novel resistant gene provided by the invention, a potential drug-resistant gene of the chloromycetin is discovered, help is provided for learning about a drug resisting mechanism of the chloromycetin and supplementing a drug resisting database, and a target point is provided for resisting drugresistance to the chloromycetin afterwards.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and particularly relates to a novel resistance gene of chloramphenicol and its application. Background technique

[0002] Chloramphenicol antibiotics can act on the 50S subunit of the bacterial ribosome and block protein synthesis. They are broad-spectrum bacteriostatic antibiotics. The 70S ribosome of bacterial cells is the main cellular component of protein synthesis, and it includes two subunits, 50S and 30S. Chloramphenicol binds reversibly to the 50S subunit, blocks the action of transpeptidylase, and interferes with the binding of aminoacyl-tRNA terminals with amino acids to the 50S subunit, thereby hindering the formation of new peptide chains and inhibiting protein synthesis . Because chloramphenicol can also combine with 70S of human mitochondria, it can also inhibit the protein synthesis of human mitochondria, which is toxic to the human body. Because the binding of chloramphenicol to th...

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