Unit cell fungus, engineering fungus prepared through motion ferment and application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of Zymomonas and engineering bacteria, which is applied in the field of Zymomonas mobilis engineering bacteria, can solve the problems of ethanol production reduction and achieve the effect of convenient screening
Inactive Publication Date: 2005-03-30
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
View PDF0 Cites 22 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] When Zymomonas mobilis uses sucrose as a fermentative carbon source, by-product sorbitol leads to reduced ethanol production
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0017] Example 1 Preparation of Zymomonas mobilis Engineering Bacteria
[0018] A. Culture of Zymomonas mobilis and DNA extraction
[0019] Zymomonas mobilis was cultured at 30 degrees Celsius for 48 hours in medium X (10% glucose, 0.5% yeast extract, 0.1% ammonium sulfate, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, pH7.0), and then used Total DNA was extracted by CTAB method for subsequent genetic manipulation.
[0020] B. Cloning of partial fragments of the glucose-fructose oxidoreductase gene of Zymomonas mobilis XW101
[0021] Using SEQ ID NO: 2 (5'-AAAGCCGGCGAATCAGATAAC-3') and SEQ ID NO: 3 (5'-TGGAAGAACCAATGACGCCT-3') as primers, the glucose-fructose oxidoreductase gene of Zymomonas mobilis was amplified. Obtain the PCR product of XW101 glucose-fructose oxidoreductase gene, its size is 1477bp, sequence is as shown in SEQ ID NO: 1, and cloned on the pGEM-T easy vector, this recombinant plasmid is named pGFO15, after sequencing analysis, The homology ...
Embodiment 2
[0050] Example 2 Engineering strain XW1021 uses sucrose to ferment ethanol
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
This invention relates to motion fermentation monocell engineering bacterium, its prepn. method and its application in fermentation for producing ethanol. In the prepn. of said engineering bacterium, the resistance gene of tetracycline is inserted into glucose-fructose oxide-erductase gene of motion fermentation monocell baterium XW101, endo zyme being used for digesting said recombination plasmid to form linear plasmid, after the transformation of motion fermentation monocell bacterium XW101, screening the strain having tetracycline resistance mutation. Carbon source is used as substrate, with this inventive engineering bacterium to produce ethanol with high ethanol prodn. yield and less by-produt-sorbicolan.
Description
technical field [0001] The invention relates to Zymomonas mobilis engineering bacteria, its preparation method, and its application in fermenting and producing ethanol. Background technique [0002] Zymomonas mobilis is the only anaerobic bacterium discovered so far that can ferment glucose, fructose and sucrose to produce ethanol through the Entner-Doudoroff (E-D) pathway. [0003] In the E-D metabolic pathway, glucose-6-phosphate dehydrogenase catalyzes glucose-6-phosphate to generate 6-phosphogluconolactone, and at the same time, reduces NAD to generate NADH. A highly catalytic lactonase rapidly hydrolyzes the lactone, and then 6-phosphogluconate dehydrogenase converts the hydrolyzed product 6-phosphogluconate into 2-keto-3-deoxy-6-phosphogluconate. 2-Keto-3-deoxy-6-phosphogluconate is a unique intermediate in the E-D pathway. Specific aldolases in turn cleave this compound into pyruvate and glyceraldehyde-3-phosphate, which are then converted to a second pyruvate molec...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more