This invention pertains to recombinant AsCpf1 and LbCpf1 nucleic acids and polypeptides for use in
CRISPR / Cpf1
endonuclease systems and
mammalian cell lines encoding recombinant AsCpf1 or LbCpf1 polypeptides. The invention includes recombinant
ribonucleoprotein complexes and CRSPR / Cpf1
endonuclease systems having a suitable AsCpf1 crRNA is selected from a length-truncated AsCpf1 crRNA, a chemically-modified AsCpf1 crRNA, or an AsCpf1 crRNA comprising both length truncations and chemical modifications. Methods of performing
gene editing using these systems and reagents are also provided.