WRKY32 regulated YFT1 expression affected tomato fruit color and application of WRKY32 in tomato quality improvement

A technology of tomato, fruit, applied in the fields of molecular biology and genetics
CN112430261AActive Publication Date: 2021-03-02SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2021-03-02

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Abstract

The invention discloses a WRKY32 regulated YFT1 expression affected tomato fruit color and application of WRKY32 in tomato quality improvement. Specifically, the WRKY32 regulates the transcription expression of the YFT1 by combining a W-box (TTGAC, -784bp--780bp) motif (GTCTA, -1306bp--1302bp) in a YFT1 promoter region (defining the first nucleotide at the upstream of an initiation codon ATG of the YFT1 as -1bp). WRKY32 down-regulation expression tomatoes (WRKY32RNAi) are created through an RNAi technology, the fruit form and chemical phenotype of the WRKY32RNAi tomatoes are analyzed, and solid genetic evidences are provided for fruit ethylene release and signal transduction inhibition, carotenoid content reduction, chromosome development and fruit softening speed delay.
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Description

technical field

[0001] The invention belongs to the fields of molecular biology and genetics, and relates to a method of WRKY32 regulating YFT1 expression to affect tomato fruit color and its application in improving tomato quality; in particular, it relates to a tomato transcription factor WRKY32 regulating ethylene signal core component gene YFT1 / EIN2 (YELLOW FRUITED TOMATO 1 / ETHYLENE INSENSITIVE 2) expression to control tomato fruit color and its application in tomato quality improvement. Background technique

[0002] Tomato (Solanum lycopersicum, 2n=24) belongs to the Lycopersicon plant of Solanaceae (Solanaceae), and is the second largest vegetable crop in the world. Tomato is also an important model plant for berry research because of its small genome, short growth cycle, and easy genetic transformation. Fruit color is not only related to the commodity appearance of tomato, but also closely related to the nutritional quality, so fruit color is an important aspect of t...

Claims

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