A liver cancer PDX standardization model base

A model library and liver cancer technology, applied in the field of liver cancer PDX standardized model library, can solve the problems of sporadic and scattered sources of liver cancer specimens, a small number of liver cancer PDX models, and no uniform standards

Inactive Publication Date: 2016-06-22
WEST CHINA HOSPITAL SICHUAN UNIV +1
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Problems solved by technology

Regarding the construction of liver cancer PDX models, the main problems are: the sources of liver cancer specimens ar

Method used

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Embodiment Construction

[0018] Clinical: Based on the clinical and pathological data of liver cancer in the Chinese population, standardize the collection and preparation of liver cancer tissue samples and body fluid samples; establish a standardized clinical sample database for liver cancer in the Chinese population, and improve clinical information such as pathological data and past treatment history;

[0019] Animals: Combined with clinicopathological classification and staging and liver cancer characteristic indicators, large-scale establishment of human liver cancer transplantation animal models (PDX) in the Chinese population;

[0020] a. Treatment of tumor specimens: Divide the harvested fresh tumor tissue specimens into three parts: the first part is cut into about 10-20mm in size under sterile conditions 3 Implanted into immunodeficient mice quickly or put into freezing medium (RPMI1640 medium containing 10% fetal bovine serum, 10% dimethyl sulfoxide) and stored in liquid nitrogen; the second...

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Abstract

Compared with human source tumor cell line models, PDX model tumors have the differentiated degree, morphological characteristics, structural characteristics and molecular characteristics closer to those of tumors of human bodies, so that an important in-vivo model is provided for biological study of tumors, searching of diagnostic markers and medicine screening. The invention provides a liver cancer PDX standardization model base which contains complete clinical information of patients with live cancer, pathologic types and grades, gene mutation and genetic transcription expression conditions; a patient primary tumor cell line corresponding to the liver cancer PDX model is established at the same time; comprehensive assessment which is nearly clinical is performed on the anti-liver cancer function of medicine on the cell level and the animal level.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a liver cancer PDX standardized model library. Background technique [0002] PDX (Patient-Derivedtumor Xenograft) human-derived tumor xenograft model refers to the transplantation of fresh tumor tissues from patients into immunodeficient mice, and relies on the microenvironment provided by the mice for growth. Compared with human tumor cell line models, the degree of differentiation, morphological characteristics, structural characteristics and molecular characteristics of PDX model tumors are closer to the characteristics of human tumors, which provides great opportunities for the biological research of tumors, the search for diagnostic markers and Drug screening provides an important in vivo model. In addition, the PDX model can reflect the characteristics of the patient's own tumor from which the specimen was derived, including the specificity of drug response. Therefore, PDX models ...

Claims

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Application Information

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IPC IPC(8): A01K67/02C12N5/09
CPCA01K67/02C12N5/0693
Inventor 肖凯魏哲钟治晖叶雪飞王莉
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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