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Fungus fusant RZ1 for degrading cellulose and application thereof

A technology for degrading cellulose, CCTCCM20211163, applied in the biological field, can solve the problems of low enzyme activity and low cellulose degradation rate related to fiber degradation, and achieve the effects of reliable bacterial resource support, good degradation effect, and high biological safety

Pending Publication Date: 2022-03-08
YANCHENG TEACHERS UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a highly efficient cellulose-degrading Penicillium-Mucor fusion son RZ1 and its application, aiming to solve the problems of low enzyme activity related to fiber degradation and low cellulose degradation rate in existing production applications

Method used

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  • Fungus fusant RZ1 for degrading cellulose and application thereof
  • Fungus fusant RZ1 for degrading cellulose and application thereof
  • Fungus fusant RZ1 for degrading cellulose and application thereof

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preparation example Construction

[0029] 3. Preparation of protoplasts

[0030] (1) Selection of parents: Penicillium and Mucor that were cultured in PDA medium for 48 hours were used as parents.

[0031] (2) Preparation of wall-breaking enzyme: a mixed enzyme solution of 1% helicase and 2% lysozyme was used as the wall-breaking enzyme.

[0032] (3) Osmotic stabilizer: 0.6mol / L sucrose solution.

[0033] (4) Enzymolysis conditions: the enzymolysis temperature is 38° C., and the enzymolysis time is 2 hours.

[0034] (5) Separation of protoplasts: Take 5 mL each of the parental bacterial liquid, centrifuge at 7000 r / min for 20 min, collect the bacterial cells, wash twice with the osmotic agent, take the precipitate, and suspend it in the osmotic agent. Add 5mL of wall-breaking enzymes, enzymolyze at 38°C for 2h, centrifuge at 7000r / min for 5min, take the supernatant, wash with osmotic agent, centrifuge at 7000r / min for 10min, wash with osmotic agent after centrifugation, and then suspend protoplasts in a stabl...

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Abstract

The invention discloses a penicillium-mucor fusant RZ1 capable of efficiently degrading cellulose and application of the penicillium-mucor fusant RZ1. According to the method, culturable fungi capable of efficiently degrading fibers are separated and screened from faeces of wild elk, fusion strains which are rich in fiber degrading enzyme variety and relatively high in enzyme activity are prepared and screened by utilizing a cell fusion technology, and the biodegradation efficiency of the strains on plant straw cellulose is to be improved; the obtained strain is named as penicillium-mucor fusant RZ1, and is preserved in China Center for Type Culture Collection on September 13, 2021, the preservation number is CCTCC M 20211163, the preservation address is Wuhan University, Luoma, Wuchang District, Wuhan, and the postcode is 430072. The penicillium-mucor fusant RZ1 has the advantages of high fiber degradation rate, high environmental tolerance, high biological safety and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cellulose-degrading Penicillium-Mucor fusion son RZ1 and its application. Background technique [0002] Cellulose is widely distributed and abundant in nature, and it is also the cheapest renewable resource. Cellulose is mainly degraded by cellulase, which is a complex enzyme, under which the cellulose is finally degraded into glucose. The mechanism of action of cellulase to degrade cellulose is the most commonly accepted theory so far. Synergy is generally considered to be the cooperation between endocellulase, exocellulase and β-glucosidase. [0003] At present, cellulase production strains generally have the problems of single type of enzyme synthesized and low enzyme activity. Cellulase production is sensitive to strain culture time, culture temperature, and initial pH value of the culture medium, and is easily inactivated if not handled properly. Therefore, it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12R1/645
CPCC12N1/14C12N9/2437
Inventor 王欢莉范忠军夏文龙邹琰懿蒋晶
Owner YANCHENG TEACHERS UNIV
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