Breeding method for solving color difference problem of colored fresh-eating corn grains

A technology of corn kernels and fresh food, which is applied in the direction of botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of inconsistent shades, large deviation between the ratio of colored kernels and the theoretical value, and impure kernel color during the fresh eating period. To achieve the effects of short breeding cycle, fast homozygosity and low cost

Active Publication Date: 2016-03-02
SHENYANG TEYIJIA CORN TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0028] The present invention aims at the problems such as impure grain color, inconsistency in depth and large deviation between the ratio of colored grains and the theoretical value caused by the difficulty in homozygous color genotypes of colored fresh edible corn inbred lines and hybrids. The chemical induction technol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1. Color purification technology of 'Shen Nong Hong Nuo' fresh waxy corn

[0052] 'Shennong Hongnuo' is a pink fresh waxy corn hybrid, which belongs to the aleurone-type colored corn. Due to the genetic complexity of the gene controlling the red aleurone layer, this variety exhibited inconsistent color at the harvest stage of fresh ears. Therefore need to purify color, reach the pure and even purpose of the seed color of milk stage.

[0053] (1) Homozygous genotypes of the two inbred lines

[0054] Using the conventional colchicine induction method, the genotypes of the female inbred line 'Hongnuo 80' and the paternal inbred line 'Hongnuo 68' were homozygous: inject colchicine inducer into the silk at the silking stage, Chromosome doubling of the female gamete directly forms diploid seeds. Seeds with different genotypes, ie different doses of color genes, are obtained after maturity. The number of induced strains was 50.

[0055] (2) Color identificat...

Embodiment 2

[0061] Example 2. Color purification technology of 'Kennian No. 1' yellow glutinous fresh corn

[0062] 'Kennian 1' is a variety of yellow-grained fresh waxy corn, but the color of the endosperm shows different shades among different kernels of the same ear. Therefore, it is necessary to purify the genotype to make the grain color uniform.

[0063] (1) Homozygous genotypes of the two inbred lines

[0064] Using the existing trifluralin herbicide induction technology, the genotypes of the female parent inbred line 'Nuo 1' and the paternal inbred line 'Nuo 2' were homozygous: Inject trifluralin inducer into the silk during the silking stage , so that the chromosomes of the female gamete directly double to form diploid seeds without double fertilization. Seeds with different genotypes, ie different doses of color genes, are obtained after maturity. The number of induced strains was 50.

[0065] (2) Color identification and screening of double inbred lines

[0066] Duri...

Embodiment 3

[0071] Example 3. Color purification technology of 'Jinghuanuo 2008' Huanuo fresh corn

[0072] ‘Jinghua Nuo 2008’ is a single-cross variety of red and white colored fresh edible waxy corn, which belongs to the aleurone-type colored corn. This variety is a hybrid of two inbred lines, Bainuo 'N203' and Hongnuo 'ZN6'. In theory, F1 grain color should be white: red = 1:3. But in fact, the grain color of this variety is white, pink, and grains with different degrees of pink halo in the fresh ear harvest period, and there are fewer colored grains and more white grains, and the white grains exceed 1 / 4 of the theory Value, darker kernels are less than 1 / 4 of the theoretical value. It is necessary to purify the genotype so that the color of the late stage grains will be darker, and the proportion of colored grains will reach the theoretical value.

[0073] (1) Purification of the genotypes of the parental inbred lines

[0074] Using the conventional colchicine induction method...

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PUM

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Abstract

A breeding method for solving a color difference problem of colored fresh-eating corn grains is characterized in that conventional colchicine or herbicide induction and other chemical induction methods are adopted, a female gamete chromosomal of an amphiphilic selfing line is induced for doubling to make genotypes including a genotype controlling grain colors highly homozygous; followed by, according to color standards of varieties, grain color and depth phenotypes are subjected to genotype identification and selection; an amphiphilic selfing line with pure and uniform colors and consistent genotypes and phenotypes is obtained, finally, pure colors and uniform color depth of hybrid grains in a fresh-eating period and a hybrid mature period are achieved, and the color ratio is consistent to a theoretical value; and the breeding method has the technical effects that the color genotypes are highly homozygous, the grain colors in the fresh-eating period are pure and uniform, and the color phenotype ratio is consistent to the theoretical value. No matter compared with conventional techniques or molecular techniques, the method has the advantages of good color effect, accurate breeding result, high breeding efficiency, fast homozygous speed, low economic cost, simple techniques, easy operation, and convenience for application.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, in particular to the breeding technology for the color of fresh corn grains such as colored waxy corn and sweet corn. Background technique [0002] In the breeding of colored fresh corn, the color and uniformity of the kernels at the fresh ear harvesting stage are inconsistent with those at the maturity stage, or the color ratio deviates greatly from the theoretical value, which has always been an unsolved technical problem in the breeding of colored fresh corn. Taking red glutinous corn (pink) single-cross as an example, although the kernel color of the F1 generation is uniform and red at the mature stage, the color of different kernels on the same ear is not consistent at the fresh ear harvesting stage, that is, the filling stage. , showing pink, pale pink, blush, a small amount of erythema, and even non-colored kernels. For another example, in the single-cross hybrid of red glutinous (...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H1/04
CPCA01H1/04A01H1/08
Inventor 史振声张喜华钟雪梅朱敏
Owner SHENYANG TEYIJIA CORN TECH
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