Building body of mediating plant fertility and application thereof

A construct and plant technology, applied in the field of constructs mediating plant fertility, can solve the problems of low sorting efficiency and poor effect

Inactive Publication Date: 2014-01-22
BEIJING DABEINONG TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to easily isolate the maintainer plants, the construct can also contain marker genes, such as color genes, but expensive machines or manual sorting are required, and the sorting efficiency is low and the effect is poor for seeds with glumes such as rice

Method used

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  • Building body of mediating plant fertility and application thereof
  • Building body of mediating plant fertility and application thereof
  • Building body of mediating plant fertility and application thereof

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0112] The first embodiment, transformation of maize plants with recombinant expression vector DBN100123 comprising male fertility gene Ms45, DAM methylase gene and herbicide resistance gene PAT

[0113] 1. Construction of recombinant cloning vectors DBN01-T, DBN02-T, DBN03-T, DBN04-T

[0114] The synthesized 5126:Ms45 fragment was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T, which was constructed Process such as figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ initiation codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; 5126 is the corn anther specific promoter (SEQ ID NO:1); Ms45 is the coding sequence of maize male fertility gene (SEQ ID NO:2); MCS is t...

no. 2 example

[0166] Second embodiment, transformation of maize plants with recombinant expression vector DBN100010 comprising male fertility gene Ms26, α-amylase gene and herbicide resistance gene PAT

[0167] 1. Construction of recombinant cloning vectors DBN05-T, DBN06-T, DBN07-T, DBN08-T

[0168] The synthesized 5126:Ms26 fragment was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN05-T, which was constructed Process such as image 3 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ initiation codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; 5126 is the corn anther specific promoter (SEQ ID NO:1); Ms26 is the coding sequence of maize male fertility gene (SEQ ID NO:16); MCS is the mul...

no. 3 example

[0220] The third embodiment, transformation of rice plants with recombinant expression vector DBN100011 comprising male fertility gene DPW, α-amylase gene and herbicide resistance gene PAT

[0221] 1. Construction of recombinant cloning vectors DBN09-T, DBN10-T, DBN11-T, DBN12-T

[0222] The synthesized gDPW fragment was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN09-T. The construction process is as follows: Figure 5 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of bacteriophage f1; LacZ is the LacZ start codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; gDPW is the rice male fertility Genome sequence (SEQ ID NO:23); MCS is multiple cloning site).

[0223] Then the recombinant cloning vector DB...

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Abstract

The invention relates to a building body of mediating plant fertility and application thereof. The building body comprises (a) a first nucleotide sequence, wherein male fertility of a plant is restored when the first nucleotide sequence is expressed; (b) a second nucleotide sequence, wherein formation or function of fertile male gametes in the plant is inhibited when the second nucleotide sequence is expressed, (3) a third nucleotide sequence, wherein inhibition or neutralization on a herbicide is formed when the third nucleotide sequence is expressed. The building body of mediating plant fertility and application thereof disclosed by the invention are applied to a method for keeping a homozygous recessive state of a male sterile plant, so that the building body is good in benefit, and simple to operate; the generated male sterile descendant is non-transgenic; the building body is convenient to identify, high in sorting efficiency, good in effect and especially applicable to the situation that the contained building body is inconveniently identified in the seed stage when the building body is applied to the method for producing seeds from the plant with female gametes and male gametes.

Description

technical field [0001] The invention relates to a construct for mediating plant fertility and its application, in particular to a construct for mediating plant fertility comprising a herbicide resistance gene and its application. Background technique [0002] The development of plant hybrid breeding technology has greatly improved the quality and quantity of crops. Various combinations of yield increases and desired traits such as disease resistance, insect resistance, heat tolerance, drought tolerance, and changes in plant composition are possible thanks to the application of hybridization techniques. Typically, the application of hybridization relies on the male parent providing pollen to the female parent, thereby obtaining hybrids. [0003] A plant is self-pollinating if pollen from one flower is transferred to the same or another flower on the same plant. A plant is cross-pollinated if the pollen comes from a flower of a different plant. Rice usually reproduces by se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/63C12N5/10A01H1/02
Inventor 于彩虹丁德荣杨进孝鲍晓明贾志伟王志新金许成张进峰夏祖灵
Owner BEIJING DABEINONG TECH GRP CO LTD
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