The invention discloses a high-efficiency
cell supernatant
Protein A purification method, and belongs to the technical field of
biology. A porous filter plate (1) is used for replacing a pre-assembled column to serve as a purification filler supporting medium,
protein A filler is loaded into a hole site, gaskets of 10 micrometers are placed above and below the filler, the gaskets do not adsorb
protein, the filler can be prevented from floating when a sample is added, the uniformity of the thickness of the filler is guaranteed, sufficient combination of
protein and the filler is guaranteed, and the protein
recovery rate is increased. And filtering the
cell supernatant by using a 0.45 mu m filter membrane, adding the filtered
cell supernatant into a porous filter plate (1) containing
protein A filler, and carrying out
impurity cleaning and
elution to collect the
target protein. Compared with a preassembled column purification mode, the method has the advantages that single-time multi-flux
filtration can be realized by using the
vacuum pump, the purification efficiency is high, and the consumed time is short; compared with a purification mode of a
protein purification instrument, the method is simple in
material consumption, low in cost, short in time consumption and suitable for high-
throughput sample treatment.