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Method for improving artificial hatching rate of tegillarca granosa

A technology of artificial hatching and mud cockles, which is applied in the fields of climate change adaptation, application, fish farming, etc., can solve the problems of increasing the fertilization rate of mud cockles and limiting the hatching rate, and reduce the fertilization rate and deformity rate of polyspermia , increase the hatching rate, the effect of simple operation

Pending Publication Date: 2022-03-22
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the disadvantages that the existing method for raising seedlings of cockles tends to increase the polyspermy fertilization rate of cockles and limit its hatching rate, and provides an efficient, convenient and cheap method for improving the artificial hatching rate of cockles

Method used

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  • Method for improving artificial hatching rate of tegillarca granosa
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  • Method for improving artificial hatching rate of tegillarca granosa

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: Experiment of the effect of water body calcium and magnesium ions on the fertilization of cockles

[0024] Time: July 2019, Location: Qingjiang Base of Zhejiang Institute of Marine Aquaculture.

[0025] Take about 500 sexually mature cockles (body weight about 9g), wash them, dry them in the shade and expose them to air at 23°C for 12 hours. Subsequently, each cockle was individually placed in a beaker filled with 500 mL of filtered seawater for artificial induction, and the male and female gamete suspensions were collected. Check the gamete quality under a microscope, measure the concentration of sperm and eggs, and discard the gamete suspension with low sperm motility and irregular egg shape. As shown in Table 1, 15 experimental groups and 1 control group were set up to compare the effects of different calcium and magnesium ion concentrations on the fertilization rate, polyspermy fertilization rate and hatching rate of cockles (10 repetitions in each gr...

Embodiment 2

[0034] Embodiment 2: Experiment of the effect of water body calcium and magnesium ions on the fertilization of cockles

[0035] Time: August 2021, Location: Qingjiang Base of Zhejiang Institute of Marine Aquaculture.

[0036] About 500 mud cockles (body weight about 8g) raised in acidified seawater conditions (pH7.8) for 30 days were washed and dried in the air at 23°C for 12 hours after cleaning the dirt on the shell surface. The cockles were individually placed in a beaker filled with 500mL acidified seawater (pH7.8) to induce production, and a high-quality gamete suspension was collected.

[0037] An experimental group (adding calcium and magnesium ions) and a control group were set up to compare the difference in the fertilization rate of cockles under the two fertilization conditions (10 repetitions in each group).

[0038] Every part of gamete suspension (20 females and 20 males) collected is equally divided into two parts, one part is transferred to a 50L acidified sea...

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Abstract

The invention relates to the technical field of marine product production, and discloses a method for increasing the artificial hatching rate of tegillarca granosa, male and female gametes of the tegillarca granosa are artificially obtained and are artificially fertilized in a seawater environment added with calcium and magnesium ions, the pH value of a water body is 8.0-8.2, the concentration of the added calcium ions is 200-300 mg / L, the concentration of the magnesium ions is 150-200 mg / L, and the sperm-egg ratio is controlled to be 100: 1-200: 1; the method is easy to operate, low in cost and short in operation time, and development and growth of the tegillarca granosa larvae are not affected.

Description

technical field [0001] The invention relates to the field of seafood production, in particular to a method for improving the artificial hatching rate of cockles. Background technique [0002] Tegillarca granosa (Tegillarca granosa) is very popular among consumers in my country because of its delicious meat and rich nutrition. Tegillarca granosa has a high yield of artificial breeding. It has become an important tidal flat cultured economic shellfish in the southeast coastal area of ​​my country, and it is also an important part of coastal tidal flat marine ecology. important part of the system. However, because the cockle adopts broadcast fertilization, it directly discharges the sperm eggs into the seawater during the breeding period and completes the whole process of fertilization, growth and development in the open water body, and the success rate of its fertilization mainly depends on the interaction of gametes in the water body effect. Zhu Junquan and others disclosed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 刘广绪施巍韩毓唐彧
Owner ZHEJIANG UNIV
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