The invention discloses a codonopsis pilosula frame building and seed harvesting method. The codonopsis pilosula frame building and seed harvesting method comprises the following steps: 1, reserving seeds, namely selecting a field with Weining codonopsis pilosula which grows strongly, is free of pest and disease damage, has thick root and large leaves as a seed reserving base, building frames by building flat frames, selecting biennial Weining codonopsis pilosula for reserving seeds, beginning to harvest seeds in a seed harvesting stage from the beginning of October of the year, and harvesting the seeds when fruits are yellowish-brown and softened, the seeds are black brown and the surfaces are bright; 2, harvesting seeds, namely arterially picking mature fruits, leaving immature parts and packing the harvested seeds by plastic bags; 3, threshing, namely putting the seeds in the paved canvas or clean cement court drying yard in strong sun days, spreading the seeds, striking the seeds by wood sticks or artificially treading the seeds by feet in the evening, repeatedly striking and opening capsules, shrugging off seed peel by wooden forks, fully leaking the seeds and threshing, screening by a sample separating screen or carrying out air separation after threshing, straightly drying for 2-3 days in the sun, and drilling until the water of the seeds reaches the standard; and 4, storing, namely using the Weining codonopsis pilosula seeds in the year, storing the seeds in dry environment with temperature controlled to 5-15 DEG C in the storage process, and packaging the seeds in dry and breathable plastic bags.