Pleurotus albophyllum strain, fruiting body and application of pleurotus albophyllum strain and fruiting body

Abstract

The invention discloses a pleurotus ostreatus strain, a fruiting body of the pleurotus ostreatus strain and application of the pleurotus ostreatus strain and the fruiting body of the pleurotus ostreatus strain. The preservation number of the pleurotus ostreatus strain is GDMCC No: 66208. The strain enriches the variety of the pleurotus albellus, and the strain is high in commodity value, tawny in pileus, provided with stripes, hard in texture and suitable for agricultural cultivation and industrial cultivation. The pleurotus ostreatus strain is good in temperature adaptability and can normally fruit when the temperature is lower than 20 DEG C, and the cultivation growing season is prolonged. The whole production cycle of the pleurotus albopicus strain is short, one production cycle can be completed within 40-45 days, and the production cost is reduced. The biotransformation rate of the pleurotus albellus strain reaches 100-140%, the economic efficiency of cultivation of the pleurotus albellus strain is greatly improved, and the production enthusiasm of farmer households is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biotechnology, and more particularly to a Pleurotus cystidiosus strain, fruiting body and application thereof. BACKGROUND

[0002] The existing Pleurotus cystidiosus has the following defects: (1) most of the Pleurotus cystidiosus in nature exist in a wild state, only a small number of which can be artificially domesticated and cultivated, and the germplasm resources are limited, which is not conducive to the popularization of the variety; (2) the Pleurotus cystidiosus is a rare fungus occurring in high-temperature seasons, and is a variety suitable for cultivation in late spring, summer and early autumn, with an optimum temperature of 27-28℃, normal development at 25-30℃, faster growth of fruiting bodies at a temperature close to 30℃, and no mushroom buds at a temperature lower than 20℃ or higher than 35℃, poor temperature adaptability; (3) long production cycle, at least 50-60 days are needed to complete a production cycle; (4) the existing process can only achieve a biological conversion rate of 70-90%, low biological conversion rate, and resource waste. Therefore, it is urgent to breed a new Pleurotus cystidiosus strain with good temperature adaptability, short production cycle and high biological conversion rate. SUMMARY

[0003] In order to solve the above technical problems, the present application provides a Pleurotus cystidiosus strain, fruiting body and application thereof, and the present application uses backcross breeding to breed a single-double hybrid of Pleurotus cystidiosus and Pleurotus eryngii and No. 2 star river white mushroom, and the hybrid offspring is further hybridized with the parent Pleurotus cystidiosus, and a new Pleurotus cystidiosus strain with good temperature adaptability, short production cycle and high biological conversion rate, i.e. star river Kirin mushroom No. 1, is obtained by culturing the mushroom.

[0004] In a first aspect, the present application provides a Pleurotus cystidiosus strain, which has a preservation number of GDMCC No: 66208.

[0005] In a preferred embodiment, the Pleurotus cystidiosus strain comprises a sequence as shown in SEQ ID NO: 5.

[0006] In a second aspect, the present application provides a Pleurotus cystidiosus fruiting body, which is cultivated from the Pleurotus cystidiosus strain of the first aspect of the present application.

[0007] In a preferred embodiment, the length of the cap of the fruiting body is 4-10 cm, for example 4 cm, 5 cm, 6 cm, 7 cm, 8 cm, 9 cm or 10 cm.

[0008] In a preferred embodiment, the stipe length of the sub-entities is 4-9 cm, for example 5 cm, 6 cm, 7 cm, 8 cm, or 9 cm.

[0009] In a preferred embodiment, the stipe length of the sub-entities is 4-9 cm, for example 5 cm, 6 cm, 7 cm, 8 cm, or 9 cm.

[0010] In a preferred embodiment, the stipe length of the sub-entities is 4-9 cm, for example 5 cm, 6 cm, 7 cm, 8 cm, or 9 cm.

[0011] In a preferred embodiment, the stipe length of the sub-entities is 4-9 cm, for example 5 cm, 6 cm, 7 cm, 8 cm, or 9 cm.

[0012] In a preferred embodiment, the cultivation period of the P. cystidiosus sub-entities is 30-35 days, for example 31 days, 32 days, 33 days, or 34 days.

[0013] In a preferred embodiment, the growth period of the P. cystidiosus sub-entities is 12-15 days, for example 13 days or 14 days.

[0014] In a preferred embodiment, the production period of the P. cystidiosus sub-entities is 42-50 days, for example 43 days, 44 days, 45 days, 46 days, 47 days, 48 days, or 49 days.

[0015] In a preferred embodiment, the average yield per unit of the P. cystidiosus sub-entities is 536.7-725.7 g, for example 550 g, 600 g, 650 g, or 700 g.

[0016] In a preferred embodiment, the bioconversion rate of the P. cystidiosus sub-entities is 102.2-138.2%, for example 110%, 115%, 120%, 130%, or 135%.

[0017] The third aspect of the present application provides a P. cystidiosus mycelium, which is obtained by culturing the P. cystidiosus strain according to the first aspect of the present application.

[0018] The fourth aspect of the present application provides a P. cystidiosus liquid medium, which is obtained by culturing the P. cystidiosus strain according to the first aspect of the present application.

[0019] The fifth aspect of the present application provides a cultivation method of the P. cystidiosus sub-entities according to the second aspect of the present application, which comprises culturing and cultivating the P. cystidiosus strain on a cultivation medium.

[0020] In a preferable embodiment, the conditions for fruiting of the Pleurotus pulmonarius strain are as follows: the fruiting room has a culture temperature of 15-20°C, an air relative humidity of 70-90%, and a carbon dioxide concentration of 400-2000 ppm, the light intensity is set to 0-100 lux for the first 1-7 days, and then the light intensity is set to 200-800 lux, the shelf lamp is opened intermittently every day, and the culture is carried out for 12-15 days.

[0021] The sixth aspect of the present application provides a culture method of the Pleurotus pulmonarius mycelium according to the third aspect of the present application or the Pleurotus pulmonarius liquid according to the fourth aspect of the present application, which comprises culturing the Pleurotus pulmonarius strain in a liquid culture medium or a solid culture medium.

[0022] The application further provides an application of the Pleurotus pulmonarius strain according to the first aspect of the present application in preparing any one of the Pleurotus pulmonarius spores, the Pleurotus pulmonarius mycelium, the Pleurotus pulmonarius liquid, and the Pleurotus pulmonarius fruiting body.

[0023] The application further provides an application of the Pleurotus pulmonarius fruiting body according to the second aspect of the present application in food processing.

[0024] The application further provides an application of the Pleurotus pulmonarius strain according to the first aspect of the present application, the Pleurotus pulmonarius fruiting body according to the second aspect of the present application, the Pleurotus pulmonarius mycelium according to the third aspect of the present application, or the Pleurotus pulmonarius liquid according to the fourth aspect of the present application in preparing any one of the immunity-enhancing drugs, the cholesterol-lowering drugs, the anti-aging drugs, the analgesic drugs, the sedative drugs, the cough-relieving and phlegm-dissolving drugs, the defecation-promoting and toxin-removing drugs, and the antihypertensive drugs.

[0025] On the basis of common knowledge in the art, the above-mentioned preferable conditions can be combined in any manner to obtain preferable examples of the present application.

[0026] Unless otherwise specified, the reagents and raw materials used in the present application are commercially available.

[0027] The positive progress effect of the present application is that:

[0028] (1) The present application provides a new Pleurotus pulmonarius strain, which enriches the types of Pleurotus pulmonarius, has high commercial value, has a yellow-brown cap with mottling, and has a hard texture, and is suitable for agricultural cultivation and industrial cultivation.

[0029] (2) The present application has good temperature adaptability and can normally fruit at a temperature lower than 20°C, thereby prolonging the cultivation growth season.

[0030] (3) The present application has a short production cycle, and a production cycle can be completed in 40-45 days, thereby reducing the production cost.

[0031] (4) The bioconversion rate reaches 100-140%, greatly improving the economic efficiency of Pleurotus cystidiosus strain cultivation and the production enthusiasm of growers.

[0032] Biological material preservation information

[0033] The Pleurotus cystidiosus strain of the present application has been preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on April 25, 2025, and the address of the preservation center is: Experimental Building 59, No. 5, 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, China, with a postal code of 510070, and the preservation number is: GDMCC No: 66208, the culture name is Pleurotus cystidiosus No. 1, and the classification name is Pleurotus cystidiosus. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 It is a fruiting body photo of Pleurotus cystidiosus No. 1.

[0035] Figure 2 It is a fruiting body photo of the parent Pleurotus cystidiosus No. 1.

[0036] Figure 3 It is a fruiting body photo of the parent Pleurotus cystidiosus No. 1.

[0037] Figure 4 It is a schematic diagram of antagonistic reaction.

[0038] Figure 5 It is a technical route of strain identification detection.

[0039] Figure 6 It is an agarose gel electrophoresis diagram, wherein: M from top to bottom is 5000, 3000, 2000, 1000, 750, 500, 250, and 100 bp.

[0040] Figure 7 It is the sequence alignment result of Pleurotus cystidiosus No. 1-1698 bp and FJ379283.1-1724 bp.

[0041] Figure 8 It is a photo of Pleurotus cystidiosus No. 1 and Pleurotus cystidiosus covering cultivation. DETAILED DESCRIPTION

[0042] The present application will be further described below by way of examples, but the present application is not limited in the scope of the examples. The experimental methods not specified in the following examples are selected according to conventional methods and conditions, or according to the instructions of the commodity.

[0043] Example 1: Source process of Pleurotus cystidiosus No. 1

[0044] (1) Parent selection: Pleurotus eryngii and Pleurotus cystidiosus No. 2 were selected as parents. Pleurotus eryngii is the fruiting body of Pleurotus eryngii brought back by the inventor from Guangdong Muhe Ecological Agricultural Development Co., Ltd. in December 2021. The parent strain Pleurotus eryngii strain with good fruiting was obtained by tissue separation of the fruiting body. Pleurotus cystidiosus No. 2 is a new strain developed by Guangdong Xinghe Edible Fungus Technology Research Co., Ltd. and has been preserved in Guangdong Microbial Culture Collection Center on November 28, 2023, with the preservation number GDMCC No: 64080.

[0045] (2) Single hypha collection: select fruiting bodies with good shape and caps about to open, cut the cap fruiting body block with gills from the edge of the cap to the point of attachment to the stem, hang it in a sterile and dry triangular flask, cultivate in the dark for 1-3 days, cultivate at 20-22℃, air relative humidity 60-70%, after white spore powder imprint is produced, perform spore dilution and plate coating operation, cultivate at constant temperature in the dark, temperature 20-22℃, air relative humidity 60-70%, cultivate for 10-20 days, when the single spore hypha grows to 0.5-1 cm, perform plate transfer culture, cultivate at constant temperature in the dark, temperature 20-22℃, air relative humidity 60-70%, cultivate for 10-20 days, select fast-growing dominant plates for test tube transfer, and at the same time, perform microscopic examination of the hyphae of the transferred plates, if there is no lock-like joint structure, it is single hypha, continue to cultivate the single hypha test tube at constant temperature in the dark, temperature 20-22℃, air relative humidity 60-70%, and preserve the hyphae after the slope is fully grown, preservation temperature 4-10℃.

[0046] (3) UV mutagenesis treatment of single hypha: in step (2), after the plate hypha transfer test tube, place the test tube at 45-55 cm from a 30W UV lamp for 1 hour, continue to cultivate at constant temperature in the dark, and preserve fast-growing single hypha.

[0047] (4) Single-double hybridization: cross-pair Pleurotus eryngii single hypha with Pleurotus cystidiosus No. 2 double hypha, activate the test tube hypha 1 day in advance, cut inoculation blocks from the edge of the colony to the plate, with a 3 cm interval between the two inoculation blocks, respectively placed at 1.5 cm from the center point of the plate, with hyphae upwards, cultivate at constant temperature in the dark, temperature 20-22℃, air relative humidity 60-70%, after the hyphae of the two colonies contact, continue to cultivate in the dark for 5-10 days.

[0048] (5) Hybridization of strains: the mycelium at the junction of two colonies is observed under a microscope, and the mycelium at the junction of the plate with the lock-like joint structure is subcultured on a plate, and incubated at a constant temperature of 20-22°C in the dark, with an air relative humidity of 60-70%, for 10-15 days. The dominant plate with fast growth is subcultured in a test tube, and the mycelium of the subcultured plate is observed under a microscope. If the mycelium has a lock-like joint structure, it is a hybrid strain. The hybrid strain is continuously incubated in a test tube at a constant temperature of 20-22°C in the dark, with an air relative humidity of 60-70%, and is preserved after the mycelium grows on the slope at a temperature of 4-10°C.

[0049] (6) Cultivation and screening: the obtained hybrid strain is subjected to cultivation and screening according to a conventional method, and the optimal hybrid offspring is obtained through fruiting test.

[0050] (7) Backcrossing: the single mycelium of the hybrid offspring is collected according to step (2), and is hybridized with the single mycelium of Pleurotus eryngii, and the obtained hybrid strain is subjected to cultivation and screening, and the new strain of Pleurotus cystidiosus with good temperature adaptability, short production cycle and high biological conversion rate, i.e., Xinghe Qilinmug 1, is obtained through fruiting test.

[0051] Example 2 Morphological characteristics and biological properties

[0052] (1) Morphological characteristics: the fruiting body photo of Xinghe Qilinmug 1 is as shown in Figure 1 . The fruiting body stem of Xinghe Qilinmug 1 is white, near-mid, partial, and rod-shaped, with a length of 4-9 cm. The gill is brown, the cap is yellow-brown with a mottled pattern, the cap flesh is hard, the cross-sectional shape is fan-shaped or partial fan-shaped, the length is 4-10 cm, the width is 5-10 cm, and the thickness is 2-3 cm. The fruiting body photos of the parent Pleurotus eryngii and Xinghe Bailengmug 2 are shown in Figure 2 , Figure 3 . As shown in Figure 1 , Figure 2 , Figure 3 , the cap color and mottled pattern of Xinghe Qilinmug 1 are obviously different from those of Pleurotus eryngii and Xinghe Bailengmug 2, which indicates that Xinghe Qilinmug 1 is a new strain different from the parents in morphological characteristics.

[0053] (2) Biological properties

[0054] Nutrition: the main required nutrients include sugars, proteins, cellulose, hemicellulose, vitamins, and minerals.

[0055] Temperature: the mycelium culture temperature is 20-25°C, and the cultivation fruiting temperature is 15-25°C.

[0056] Light: the mycelium does not require light, and the light intensity required during the fruiting period is 0-800 lux.

[0057] Moisture: the relative humidity of air in mycelium culture is 60-70%, the moisture content of mycelium culture medium is 60-65%, and the relative humidity of air in cultivation house is 70-90%.

[0058] Air: good ventilation and fresh air are required, and the carbon dioxide concentration is 400-3000 ppm.

[0059] Time requirement: generally 40-45 days are required from inoculation to completion of fruiting.

[0060] Example 3 Antagonistic reaction

[0061] Detection basis: the inoculation, culture and observation of the sample to be detected are carried out according to the Agricultural Industry Standard of the People's Republic of China "NY / T 1845-2010 Edible Fungus Strain Distinctive Identification Antagonistic Reaction".

[0062] The Xinghe Pleurotus cornucopiae No. 1 strain obtained in Example 1 was subjected to antagonistic reaction with the parent Pleurotus eryngii strain and the Xinghe Leucopaxillus No. 2 strain.

[0063] (1) Culture medium: PDA medium (potato dextrose agar medium), 200 g of potato (using infusion juice), 20 g of glucose, 20 g of agar, 1000 ml of water, pH natural, poured into a culture dish and cooled to form a flat plate.

[0064] (2) Inoculation and culture: the inoculation combination was divided into 5 groups, Pleurotus eryngii and Pleurotus eryngii, Xinghe Leucopaxillus No. 2 and Xinghe Leucopaxillus No. 2, Xinghe Pleurotus cornucopiae No. 1 and Xinghe Pleurotus cornucopiae No. 1, Xinghe Pleurotus cornucopiae No. 1 and Pleurotus eryngii, and Xinghe Pleurotus cornucopiae No. 1 and Xinghe Leucopaxillus No. 2. Strictly according to aseptic operation, 0.2 cm x 0.2 cm inoculation blocks were cut from the edge of the strain colony after activation, one inoculation block was inoculated, the two inoculation blocks were spaced 3 cm apart, and were respectively placed at 1.5 cm from the center point of the flat plate with the mycelium facing up, each group was repeated 3 times, and was respectively subjected to confrontation culture, the culture temperature was 24-26℃, and the culture was ventilated and light-avoiding for 14 days.

[0065] (3) Observation and judgment of antagonistic reaction

[0066] Under the light, the mycelial reaction at the junction of the two strain colonies on the surface of the culture was observed, and the results of the antagonistic reaction are shown in Table 1. Figure 4 Pleurotus eryngii and Pleurotus eryngii, Xinghe Leucopaxillus No. 2 and Xinghe Leucopaxillus No. 2, and Xinghe Pleurotus cornucopiae No. 1 and Xinghe Pleurotus cornucopiae No. 1 did not present one of the three reactions of raised type, groove type and isolation type on the surface of the culture, indicating that Pleurotus eryngii, Xinghe Leucopaxillus No. 2 and Xinghe Pleurotus cornucopiae No. 1 did not present antagonistic reaction by themselves. Xinghe Pleurotus cornucopiae No. 1 and Pleurotus eryngii, and Xinghe Pleurotus cornucopiae No. 1 and Xinghe Leucopaxillus No. 2 presented isolation type reaction on the surface of the culture, which had antagonistic reaction, indicating that Xinghe Pleurotus cornucopiae No. 1, Pleurotus eryngii and Xinghe Leucopaxillus No. 2 were different strains.

[0067] Strain identification detection of Example 4

[0068] Genomic DNA of Star River D. boninensis No. 1 was extracted by column method and magnetic bead method, and PCR amplification and sequencing were performed with NS1, NS8, ITS1 and ITS4 primers. The sequencing results were subjected to NCBI-BLAST comparison.

[0069] (1) The experimental instruments, reagents and consumables involved are shown in Table 1.

[0070] Table 1 Main instruments, reagents and consumables

[0071]

[0072] (2) The technical route used is shown in Figure 5 , wherein the primer information is shown in Table 2, and the PCR amplification reaction system and conditions are shown in Table 3.

[0073] Table 2 Primer information

[0074]

[0075]

[0076] Table 3 PCR amplification reaction system and conditions

[0077]

[0078] (3) Detection results

[0079] The results of agarose gel electrophoresis are shown in Figure 6wherein the well No. 1 amplification primer and sequencing primer are ITS1 and ITS4, and the well No. 2 amplification primer and sequencing primer are NS1 and NS8, and the obtained sequencing result of No. 1 of Star River Pleurotus cornucopiae is obtained after two-way splicing to obtain the sequence of No. 1 of Star River Pleurotus cornucopiae-1698bp (SEQ IDNO:5) : TCTAGTATAAACAAATTTGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTATTTGATGGTACCTTGCTACATGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATCAAGCCCCGACTTCTGGAAGGGGTGTATTTATTAGATAAAAAACCAACGCGGCTCGCCGCTCCCTTGGTGATTCATAATAACTTCTCGAATCGCATGGCCTTGTGCCGGCGATGCTTCATTCAAATATCTGCCCTATCAACTTTCGATGGTAGGATAGAGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTAGTGACAATAAATAACAATATAGGGCTCTTTTGGGTCTTATAATTGGAATGAGTACAATTTAAATCCCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACTTCAGACCTGGCTGGGCGGTCCGCTTAACGGCGAGTACTGTCTGGCTGGGCCTTACCTCTTGGTGAGCCGGCGTGCCCTTTATTGGTGTGCGTTGGGGAACCAGGACTTTTACCTTGAGAAAATTAGAGTGTTCAAAGCAGGCCTGTGCCTGAATACATTAGCATGGAATAATAAAATAGGACGTGCGGTTCTATTTTGTTGGTTTCTAGAGTCGCCGTAATGATTAATAGGGATAGTTGGGGGCATTGGTATTGAGTCGCTAGAGGTGAAATTCTTGGATTGACTCAAGACCAACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAGGTTAGGGGATCGAAAACGATCAGATACCGTTGTAGTCTTAACAGTAAACTATGCCGACTAGGGATCGGGCAATCTCAAACATGATGTGTTGCTCGGCACCTTACGAGAAATCAAAGTCTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGTGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACCAGGTCCAGACATAACTAGGATTGACAGATTGATAGCTCTTTCATGATTTTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGATAACGAACGAGACCTTAACCTGCTAAATAGCCAGGCCGGCTTTCGCTGGTCGCCGGCTTCTTAGAGGGACTGTCAGCGTCTAGCTGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGACAGAGCCAGCGAGTTTTTTTCCTTGGCCGGAAGGTCTGGGTAATCTTGTGAAACTCTGTCGTGCTGGGGATAGAGCATTGCAATTATTGCTCTTCAACGAGGAATACCTAGTAAGCGTGAGTCATCAGCTCGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCTTTGGGGAGCCGGCGACGGCACCCTATTGCTGAGAAGTTGATCAAACTTGGTCATTAGAGGAA.

[0080] The NCBI-BLAST alignment results of the Xinghe Pleurotus 1 are shown in Table 4. The alignment results are only presented for the first 3 sequences in the NCBI database alignment, in which the sequence ID FJ379283.1 has the highest sequence identity. The alignment results of Xinghe Pleurotus 1-1698bp and FJ379283.1-1724bp sequences are shown in Figure 7 , and the alignment results of Xinghe Pleurotus 1-1698bp and FJ379283.1-1724bp sequences are shown in Figure 7It can be seen that 1696bp can match 1695bp, only the 615th site A of 1698bp of Xinghe Qilunjunmushan No. 1 and the 627th site T of 1724bp of FJ379283.1 do not correspond, and the sequence consistency reaches 99%. The corresponding strain of FJ379283.1 is Pleurotus cystidiosus, which indicates that Xinghe Qilunjunmushan No. 1 has the closest genetic relationship with the Pleurotus cystidiosus strain, and Xinghe Qilunjunmushan No. 1 is Pleurotus cystidiosus (reference Chinese name: bubble capsule side ear).

[0081] Table 4 comparison results

[0082]

[0083]

[0084] Note: XHQLG1hao: Xinghe Qilunjunmushan No. 1.

[0085] Example 5 cultivation experiment

[0086] Xinghe Qilunjunmushan No. 1 and the parent Pleurotus eryngii, Xinghe Bailinmushan No. 2 were cultivated in bags.

[0087] (1) Mycelium propagation: the mother seed was transferred to a PDA plate, and cultured in a constant temperature incubator at 21℃ and air relative humidity of 60-70% in the dark. After 10 days, it was inoculated into a 700ml triangular flask liquid culture medium at 23℃ in the dark for 5 days, and then inoculated into a fermentation tank liquid culture medium after forming small down balls at 18-23℃ in the dark for 5 days. The liquid culture medium formula is: glucose 5%, sucrose 5%, soybean meal 0.5%, peptone 0.5%, magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.2%, and pH is natural.

[0088] (2) Preparation of cultivation species: the fermentation tank liquid seed was inoculated into the bag culture medium at an amount of 30ml per bag, and cultured in the dark in a culture room at 20-25℃, air relative humidity of 60-70%, and carbon dioxide concentration of 2000-3000ppm for 30 days. The bag culture medium formula is: corn cob 18%, mixed wood chips 43%, bran 19%, rice bran 10%, corn meal 10%, and water content 65%, and the material weight is 1500g.

[0089] (3) Pinhead management: two pinhead positions are cut on the bag wall with a film cutter, and the cultivation is carried out in a pinhead house at 15-20℃, air relative humidity of 70-90%, carbon dioxide concentration of 400-2000ppm, light intensity of 0-100 lux for the first 1-7 days, and light intensity of 200-800 lux thereafter, the layer rack lamp is opened intermittently every day, and the cultivation is carried out for 12-16 days, and the fruiting body cap is fully unfolded, but the edge of the cap is still slightly curled when harvesting.

[0090] The results of the bag cultivation and pinhead experiment of Star River Pleurotus eryngii No. 1 and the parent Pleurotus eryngii and Star River White Mushroom No. 2 are shown in Table 5, and the corresponding pinhead photos are shown in Figure 1 、 Figure 2 、 Figure 3 .

[0091] Table 5: Results of bag cultivation experiment of Star River Pleurotus eryngii No. 1 and the parent

[0092]

[0093] As shown in Table 5, under the same cultivation period and control parameters, the production period of Star River Pleurotus eryngii No. 1 is the shortest, and the yield per unit area and biological conversion rate are the highest, as shown in Figure 1 、 Figure 2 、 Figure 3 The cap color and pattern of the three strains are different, which shows that the Star River Pleurotus eryngii No. 1 strain is a new strain different from the parent, and has obvious advantages in production period and biological conversion rate, good temperature adaptability, normal pinhead at below 20℃, and suitable for factory cultivation.

[0094] Example 6: Cultivation experiment

[0095] The Star River Pleurotus eryngii No. 1 and Pleurotus eryngii are cultivated by covering soil.

[0096] (1) The cultivation species of Star River Pleurotus eryngii No. 1 and Pleurotus eryngii are obtained in the manner of Example 5.

[0097] (2) Pinhead management: the bag and sleeve ring of the cultivation species are removed, and the bag is vertically placed in the soil, and the surface is covered with 1-2cm of soil, and the cultivation is carried out in a pinhead house at 15-20℃, air relative humidity of 70-90%, carbon dioxide concentration of 400-2000ppm, light intensity of 0-100 lux for the first 1-7 days, and light intensity of 200-800 lux thereafter, the layer rack lamp is opened intermittently every day, and the cultivation is carried out for 13-17 days, and the fruiting body cap is fully unfolded, but the edge of the cap is still slightly curled when harvesting.

[0098] The results of the soil covering cultivation and pinhead experiment of Star River Pleurotus eryngii No. 1 and Pleurotus eryngii are shown in Table 6, and the corresponding pinhead photos are shown in Figure 8 .

[0099] Table 6 Experimental results of Star River Pleurotus cornucopiae No. 1 and Pleurotus eryngii soil cultivation

[0100]

[0101] From Table 6, under the same culture period and regulation parameters, the production period of Star River Pleurotus cornucopiae No. 1 is the shortest, the yield per unit area and the biological conversion rate are the highest, and the yield per unit area is 1.5 times that of Pleurotus eryngii. Figure 8 It can be known from Table 6 that the cap colors of the two strains are obviously different, and Star River Pleurotus cornucopiae No. 1 grows faster and has better uniformity than Pleurotus eryngii. It is shown that the Star River Pleurotus cornucopiae No. 1 strain has the characteristics of short production period and high biological conversion rate, and is suitable for agricultural cultivation.

Claims

1. A Pleurotus cystidiosus, having a preservation number of GDMCC No:66208; preferably, the Pleurotus cystidiosus comprises a sequence as shown in SEQ ID NO:

5.

2. A Pycnoporus sanguineus fruit body, characterized in that, The Pleurotus cystidiosus fruiting body is cultivated from the Pleurotus cystidiosus strain of claim 1.

3. The P. cystidiosus of claim 2, wherein, The cap length of the fruiting body is 4-10 cm, the width is 5-10 cm, the thickness is 2-3 cm, and / or the stem length is 4-9 m, and / or the cap is yellowish brown with mottling. Preferably, the cultivation period of the Pleurotus cystidiosus fruiting body is 30-35 days, and / or the growth period is 12-15 days, and / or the production period is 42-50 days, and / or the average yield per unit is 536.7-725.7 g, and / or the biological conversion rate is 102.2-138.2%.

4. A Podospora curadica hypha, characterized in that, The Pleurotus cystidiosus mycelium is cultivated from the Pleurotus cystidiosus strain of claim 1.

5. A liquid of P. cystidiosus, characterized in that, The Pleurotus cystidiosus mycelium is cultivated from the Pleurotus cystidiosus strain of claim 1.

6. The cultivation method of Pleurotus cystidiosus according to Claim 2 or 3, wherein The cultivation comprises culturing the Pleurotus cystidiosus strain on a cultivation medium, and preferably, the conditions for fruiting of the Pleurotus cystidiosus strain are as follows: the fruiting body is cultured in a fruiting room with a culture temperature of 15-20℃, an air relative humidity of 70-90%, and a carbon dioxide concentration of 400-2000 ppm, the light intensity is set to 0-100 lux for the first 1-7 days, and then the light intensity is set to 200-800 lux, the shelf lamp is turned on intermittently every day, and the culture is carried out for 12-15 days.

7. The method for culturing the P. cystidiosus mycelium of claim 4 or the P. cystidiosus broth of claim 5, wherein The cultivation comprises culturing the Pleurotus cystidiosus strain on a liquid medium or a solid medium. 8.The Pleurotus cystidiosus strain of claim 1 is used for preparing any one of Pleurotus cystidiosus spores, Pleurotus cystidiosus mycelium, Pleurotus cystidiosus mycelium, and Pleurotus cystidiosus fruiting body. 9.The Pleurotus cystidiosus fruiting body of claim 2 or 3 is used in food processing. 10.The Pleurotus cystidiosus strain of claim 1, the Pleurotus cystidiosus fruiting body of claim 2 or 3, the Pleurotus cystidiosus mycelium of claim 4, or the Pleurotus cystidiosus mycelium of claim 5 is used for preparing any one of immunity-enhancing drugs, cholesterol-lowering drugs, anti-aging drugs, analgesic drugs, sedative drugs, cough and phlegm-relieving drugs, defecation and detoxification drugs, and antihypertensive drugs.

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