44 results about "Midgut" patented technology

A kind of production method of pine caterpillar type polyhedrosis virus oil preparation, the viral polyhedron of susceptible pine caterpillar midgut is separated from worm body, and separates from other solid phase substances, then process and concentrate, add solvent ethylene glycol, The suspending agent sodium dodecylbenzene sulfonate is prepared by mixing, stirring and grinding. Its pine caterpillar polyhedrosis virus has high purity, small particle size, and good suspension effect, which solves the problems of sedimentation and layering and blocking nozzles, thereby meeting the requirements of ultra-low volume spraying. The pine caterpillar polyhedrosis virus made according to this method Body virus oil agent, insecticidal rate of more than 92%, its production cost is low, does not pollute the environment, is safe for humans and animals, and has good economic and social benefits.

The invention discloses a method for large-scale quick separation of insect peritrophic membranes. The method can separate peritrophic membrane organizations from insect midgut in a short time, and then food residues therein are quickly cleaned, so that a large amount of purified peritrophic membrane organizations are obtained; the efficiency is improved by 16 times when compared with that of the general method; and the convenience is provided to the subsequent research of proteins in the peritrophic membranes.

Cell populations of intestinal midgut endoderm cells and methods of generating the cells expressing markers characteristic of intestinal endoderm lineage are disclosed. Methods of treating conditions such as diabetes are also disclosed.

The invention provides a method for extracting cockroach gathering pheromone. The method comprises the steps of feeding the cockroaches for 5-10 days, disecting midguts of the cockroaches, grinding and triturating the midgets together with feces of the cockroaches, adding 1.5-2.5 fold amounts of an organic solvent, carrying out extraction for 5-8 hours by virtue of a Soxhlet extraction method, and filtering, so as to obtain an extract. The invention further provides cockroach-killing gel bait. The cockroach-killing gel bait comprises the following components in parts by weight: 0.01-0.5 part of fipronil or a fipronil derivative, 0.05-0.5 part of the cockroach gathering pheromone, 5-30 parts of glucose syrup, 5-30 parts of sorbitol, 1-15 parts of butter, 1-15 parts of cooked flour, 1-10 parts of maltodextrin, 0.5-10 parts of azone, 0.3-2 parts of Carbopol 940, 0.3-2 parts of triethanolamine, 0.1-1.5 parts of sodium benzoate and 30-60 parts of jelly. The invention further provides a preparation method of the cockroach-killing gel bait.

The invention relates to a simple method for rapidly screening substances effective to insects. According to the method, the insects are taken as a target, candidate substances are applied to midguts of the insects, changes of the insects are observed, and the biological activity of the candidate substances for the insects is determined. The method is low in cost, good in repeatability and high in accuracy, special instruments are not required, and great convenience is provided for rapid, accurate and efficient screening of bioactive substances effective to the insects.

The invention discloses a fluorescent substrate for detecting the activity of trypsin acting on Cry1A protoxin and an application of the fluorescent substrate. The fluorescent substrate consists of fluorescence quenching group-Gly-GLy-Glu-Arg-Ile-Glu-Thr-Gly-Glu-fluorescence group. The invention also discloses the application of the fluorescent polypeptide substrate R28 in detecting the activity of insect midgut trypsin which directly acts on activated bacillus thuringiensis Cry1A protoxin. Compared with a conventional protease detection method, the fluorescent substrate disclosed by the invention has definite specificity and pertinence for detecting the activity of the insect midgut trypsin which directly acts on the activated bacillus thuringiensis Cry1A protoxin and detecting the activity of the trypsin which only acts on the corresponding fluorescent substrate R28, and intense fluorescence signals are generated in an enzymatic hydrolysis reaction, so that the sensitivity of the detection is greatly improved.

The invention discloses an in vitro cultivation method for insect mesenteron cells; the vitro cultivation method adds TNM-FH culture medium which contains conditional medium and fetal calf serum into the obtained mesenteron cells; the invention provides a simple and effective cultivation method for the insect mesenteron cells, which is suitable for in vitro cultivation of the insect mesenteron cells, and physiological and pathological research of mesenteron. The cultivation method comprises enzyme liquid used for dissociating mesenteron cells and suitable culture medium used for maintaining in vitro state of the cells, as well as serum concentration and additive, so that the survival rate of the mesenteron cells just separated is more than 80%, the survival rate of the cells after 60 d of in vitro cultivation is more than 20%; the mesenteron cells under in vitro state can be infected by karyotype polyhedral body virus, and is capable of secreting alkaline phosphatase; the in vitro cultivation method lays a foundation for researching physiological function of insect mesenteron.