44 results about "Midgut" patented technology

The invention provides a method for extracting cockroach gathering pheromone. The method comprises the steps of feeding the cockroaches for 5-10 days, disecting midguts of the cockroaches, grinding and triturating the midgets together with feces of the cockroaches, adding 1.5-2.5 fold amounts of an organic solvent, carrying out extraction for 5-8 hours by virtue of a Soxhlet extraction method, and filtering, so as to obtain an extract. The invention further provides cockroach-killing gel bait. The cockroach-killing gel bait comprises the following components in parts by weight: 0.01-0.5 part of fipronil or a fipronil derivative, 0.05-0.5 part of the cockroach gathering pheromone, 5-30 parts of glucose syrup, 5-30 parts of sorbitol, 1-15 parts of butter, 1-15 parts of cooked flour, 1-10 parts of maltodextrin, 0.5-10 parts of azone, 0.3-2 parts of Carbopol 940, 0.3-2 parts of triethanolamine, 0.1-1.5 parts of sodium benzoate and 30-60 parts of jelly. The invention further provides a preparation method of the cockroach-killing gel bait.

The invention provides a rapid acquisition method of a lepidopterous larvae midgut. The rapid acquisition method of the lepidopterous larvae midgut belongs to the technical field of entomotomy, and comprises the steps of cutting off a third uromere to a sixth uromere of a lepidoptera insect with absent vital signs, pulling the midgut out from the cut-off uromeres, putting the midgut in normal saline, separating food debris encapsulated by a peritrophic membrane from the midgut, and washing, thus obtaining the lepidopterous larvae midgut. According to the method provided by the invention, the complete midgut tissue can be obtained, the whole operation process is short, the time can be saved by 50 percent compared with a traditional operation method, and the method is applicable to three-year-old and more than three-year-old lepidopterous larvae.

The invention discloses a method for large-scale quick separation of insect peritrophic membranes. The method can separate peritrophic membrane organizations from insect midgut in a short time, and then food residues therein are quickly cleaned, so that a large amount of purified peritrophic membrane organizations are obtained; the efficiency is improved by 16 times when compared with that of the general method; and the convenience is provided to the subsequent research of proteins in the peritrophic membranes.

The invention discloses a fluorescent substrate for detecting the activity of trypsin acting on Cry1A protoxin and an application of the fluorescent substrate. The fluorescent substrate consists of fluorescence quenching group-Gly-GLy-Glu-Arg-Ile-Glu-Thr-Gly-Glu-fluorescence group. The invention also discloses the application of the fluorescent polypeptide substrate R28 in detecting the activity of insect midgut trypsin which directly acts on activated bacillus thuringiensis Cry1A protoxin. Compared with a conventional protease detection method, the fluorescent substrate disclosed by the invention has definite specificity and pertinence for detecting the activity of the insect midgut trypsin which directly acts on the activated bacillus thuringiensis Cry1A protoxin and detecting the activity of the trypsin which only acts on the corresponding fluorescent substrate R28, and intense fluorescence signals are generated in an enzymatic hydrolysis reaction, so that the sensitivity of the detection is greatly improved.

The invention provides an enteral nutrition preparation for balancing nutrition, which comprises the following raw materials in parts by weight: 245-255 parts of milk, 45-55 parts of rice water, 20-30 parts of tomato juice, 115-125 parts of rape juice, 10-15 parts of egg yellow, 45-55 parts of egg white, 10-15 parts of lean meat, 10-15 parts of chicken liver, 25-35 parts of farina, 3-7 parts of vegetable oil and 0.5-1.5 parts of salt. The enteral nutrition preparation can meet a demand of a functional dyspepsia patient, improves the tolerance of a gastrointestinal tract, effectively prevents and treats malnutrition, cannot generate an adverse effect on blood fat or blood glucose, cannot cause a burden on a liver or kidney function, has good safety, and can be applied by the patient for a long term.

The present invention discloses a human-derived insecticidal gene and insecticidal peptide encoded by the same and application thereof. The nucleotide sequence of the human-derived insecticidal gene is as represented by SEQ ID NO.1. The amino acid sequence of the insecticidal peptide encoded by this gene is as represented by SEQ ID NO.2. The insecticidal peptide may be expressed through prokaryotic system. The primary culture has binding activity to Cnaphalocrocis medinalis midgut peritrophic membrane specific receptor BBMV. It is obtained without animal immunization and has a short production cycle and a small amino acid sequence. It is suitable for in vitro mass production and may lower the safety risks resulting from wide use of existing Bt toxins and even might substitute Bt to biologically control agricultural pests in the future. It has important scientific and practical significance to reducing the use of insecticides.

The present invention is drawn to a safe, cost-effective, environmentally-friendly and ecologically-sound bioengineered pest eradication product and uses thereof. Immunological and genetic engineering techniques are used to generate monoclonal antibodies as well as viruses (phage) that display scFv heavy and/or light chain Ig fragments which exhibit high-avidity specific binding to cells of the microvilli of the midgut of imported fire ant queens. The specific monoclonal antibodies and phage displayed antibody Fab fragments are conjugated to a toxin for targeted delivery and destruction of imported fire ant queens, but not native species.

The invention discloses an insecticide for green vegetables. The insecticide is prepared from the following components in parts by weight: 5 to 10 parts of abamectin, 40 to 60 parts of fermenting solution, 3 to 8 parts of surfactant, 2 to 10 parts of activator, and 0 to 6 parts of pure alkaline. The insecticide for the green vegetables has the beneficial effects that the limitation that the insecticide mainly enters into leaves via air pores in the leaves, hydrophilic small pores in cuticles of the leaves, and cytoplasm ectodesma of leaves is broken through, and the utilization rate is greatlyincreased; after an insect eats the leaves which absorb the insecticide, the active components in the insecticide directly act onto the corpus adiposum and midgut cell of insect larvae, and are quickly duplicated to cause the disease infection and death of the larvae; the pest population and injury can be effectively controlled by transversely infecting in the populations to cause epidemic diseases and longitudinally infecting to kill pupae and ova.

Compounds that inhibit digestion in blood-ingesting pests are described herein. In one embodiment, the compounds described herein block entry of blood into the midgut and thereby inhibit digestion and nutrient processing. In another embodiment, the compounds described herein prevent pathogens contained in the blood meal from entering the midgut where they could cross the epithelial cell layer and infect the mosquito. The compounds can be administered to a population of blood-ingesting pests, such as mosquitos, directly or indirectly in an effective amount to prevent mosquitoes from transmitting diseases such as malaria, dengue fever, West Nile virus and lymphatic filariasis. Preferably, the compounds are lethal to blood-ingesting pests. The compounds can be combined with one or more excipients to prepare compositions.

The invention discloses a test method of developmental toxicity of silkworms by environmental estrogen. The method includes following steps: firstly, injecting a test sample containing a series of concentrations to 5-age silkworm larvae through 2-hour starvation treatment by employing oral gavage; after 20 minutes, performing anatomy on the silkworms through gavage, and taking tissues and organs;determining the developmental toxicity of the silkworms by the test sample through comparison of toxic endpoints of an infected group and a control group, wherein the toxic endpoints comprise a glutathione reductase activity coefficient and an anti-superoxide anion free radical activity of midguts of the silkworms; and researching the influence of the environmental estrogen 4-nonylphenol on the development of the midguts of the silkworms in a larva stage to determine a dose-effect relation between the environmental estrogen and the developmental toxicity of the silkworms in the larva stage. Byemploying the technical scheme, multiple biological effect endpoints can be generated for the environmental estrogen nonylphenol, potential risks of the silkworms exposed in an estrogen environmentalconcentration level are evaluated, and the method is a relatively fast, simple and sensitive detection method.

The invention discloses a cell line that is derived from an insect midgut tissue and is highly sensitive to baculovirus. The invention also discloses an establishment method of the cell line, and application of the cell line in baculovirus large-scale growth. The cell line can be used for replication of the virus, large-scale production of baculovirus insecticides, and construction of a baculovirus expression vector system to express protein with commercial or scientific value. Therefore, the chilo suppressalis larva midgut cell line has extremely high commercial and the scientific research value.

The invention relates to a simple method for rapidly screening substances effective to insects. According to the method, the insects are taken as a target, candidate substances are applied to midguts of the insects, changes of the insects are observed, and the biological activity of the candidate substances for the insects is determined. The method is low in cost, good in repeatability and high in accuracy, special instruments are not required, and great convenience is provided for rapid, accurate and efficient screening of bioactive substances effective to the insects.

The invention discloses a method for isolated culture of intestinal epithelial cells of Takifugu obscurus and establishment of a stress model. According to the technical scheme, firstly, feed treated with antibiotics is fed in the takifugu obscurus breeding process; secondly, selecting the midgut part of the intestinal tract of takifugu obscurus; then, by optimizing a high-efficiency isolated culture technology of the Takifugu obscurus intestinal epithelial cells and cell primary culture conditions, the Takifugu obscurus intestinal epithelial cells which are relatively high in purity and good in growth are obtained; and finally, utilizing cortisol to stimulate intestinal epithelial cells of Takifugu obscurus so as to establish the oxidative stress model. The invention provides a powerful research material for application of fugu obscurus in research of developmental biology, immunology, toxicology, environment detection and the like.

Recent advances of the research on synergistic effect of a mixed baculovirus infection demonstrated the presence of viral molecules enhancing the early event of infection. The enhancins from the Trichoplusia ni was identified to have such a function, i.e., disrupting the structural integrity of peritrophic membrane of midgut of T. ni larva. The enhancin gene was ligated downstream of the CaMV 355 promoter of a binary vector pBI121. With a drug resistant gene, the gene was introduced to a piece of tobacco leaf, from Nicotiana tobacum cv. Havana SR1. We screened 11 regenerated plants out of 37 by feeding tobacco powder mixed in artificial diet, to 3rd instar Pseudaletia separata larvae. The larval stage was usually delayed from 1 to 3 days in comparison of that of control larvae. The larvae did not pupate normally. The larvae showed irregular morphology of half larva and half pupae, suggesting a hormonal disturbance caused by the transgenic tobacco. The introduction of an enhancin gene into plants is an effective method of protecting them from insects due to the disruption of their normal life cycle.