The invention relates to the technical field of biological detection, in particular to a primer probe combination, a kit and a method for identifying Solenopsis invicta through fluorescent RPA (
recombinase polymerase amplification). The primer probe combination for identifying the Solenopsis invicta through the fluorescent RPA comprises a primer F1, a primer R1 and a probe P1. Wherein the
nucleotide sequence of the probe P1 is SEQ ID NO: 3, and
thymine at the position, 27bp away from the 5'end, of the probe P1 is modified by a
fluorophore FAM; a basic group at the position, 28bp away from the 5'end, of the probe P1 is replaced by dSpacer,
thymine at the position, 30bp away from the 5 'end, of the probe P1 is modified by a
quenching group BHQ1, and the 3' end of the probe P1 is connected with ddC. The primer probe combination is used for identifying the Solenopsis invicta through fluorescent RPA and has the advantages of being good in specificity and high in sensitivity.