Pest space repellents based on different volatility for controlling pests, especially ants

Abstract

The present invention belongs to the field of pest control, particularly the field of ant-fire ant control. The present invention discloses a pest repellent containing the following volatile compounds as pest repellent components: aldehydes with repellent activity, ketones or esters with repellent activity, linear or branched alkanols and aromatic alcohols, phenols, and ethers with repellent activity. The present invention also provides the use of the above-mentioned pest repellent for repelling pests and ants, especially fire ants. The present invention not only has a high repellent effect on fire ants and a long-lasting effect, but also has the advantages of being simple to prepare, low cost, green and efficient, non-resistant, environmentally friendly, safe for humans and animals, and naturally environmentally friendly.

Description

Technical Field

[0001] The present invention belongs to the field of pest control, in particular the field of ant control, namely fire ant control, and belongs to a component for reducing the harmful effects of effective substances on organisms other than pests. It specifically relates to the application of volatile composition essential oil in the preparation of fire ant repellent. Background Art

[0002] The red imported fire ant (Solenopsis invicta Buren), commonly known as the Red Imported Fire Ant (REFA), belongs to the genus Solenopsis, order Hymenoptera, family Formicidae, subfamily Myrmicinae. It is one of the most destructive invasive species listed by the World Conservation Union (IUCN) and a typical agricultural and forestry pest. It is native to the Paraná River basin in South America, including Brazil, Paraguay, and Argentina.

[0003] The current problems are as follows:

[0004] Red imported fire ants (RIFAs) have a strong ability to survive and reproduce, spread through multiple pathways, and lack natural enemies. They prefer to nest in areas with abundant water and sunlight, making them prone to rapid outbreaks in coastal provinces. RIFAs have a varied diet and are highly foraging. They are highly aggressive social insects, causing severe damage to the ecosystems of areas they invade. RIFAs are highly aggressive and can actively attack and bite humans. In areas where RIFAs occur, they invade homes, schools, lawns, and other areas, where human contact is common, and bites are common. The venom released by their stingers can cause redness, swelling, and blistering of the skin, accompanied by burning pain. Some bites have even resulted in serious consequences such as allergic reactions and death.

[0005] The red fire ant is also a serious pest that severely impacts agricultural production. Not only do they feed on crops, causing direct economic losses, but they also disrupt agricultural operations. The mounds of soil formed by their nests hinder mechanized agricultural operations, damaging irrigation systems and machinery, rendering agricultural facilities ineffective and reducing productivity. In public life, red fire ants can also pose a significant threat. The magnetic field generated by electric current attracts ants, causing them to gather in large numbers, resulting in short circuits in electrical wiring, damage to electronic equipment, and other public safety issues.

[0006] Unlike common ants, this "deadly" ant is the red imported fire ant, recognized as one of the 100 most dangerous invasive species worldwide. Villagers have experienced shock after being stung by red imported fire ants. These ants migrate rapidly, quickly adapting to changes in food availability, ambient temperature, and humidity. This creates a nuisance for residents, especially farmers working in the fields. Preemptive measures are urgently needed to prevent accidental injuries. A spatial repellent or a volatile spray for fabric application is needed to prevent accidental bites before working in fields or ponds.

[0007] The main prevention and control measures for red imported fire ants at home and abroad and their shortcomings:

[0008] Red imported fire ants are remarkably resilient, with strong adaptability and migration abilities. Therefore, their prevention and control are extremely important. Currently, there are two main approaches to controlling red imported fire ants: chemical control and biological control. Chemical control is the most effective and widely used, primarily involving nest dredging with pesticides and bait trapping. Nest dredging with pesticides involves spraying, dredging holes, or spreading powders directly on individual ant nests. This method allows the pesticide to reach the ants quickly, resulting in rapid killing. However, this method is labor-intensive and requires a large amount of pesticide, which can cause some ecological pollution.

[0009] The poison bait method primarily uses food favored by fire ants as a carrier, along with an attractant. A slow-release stomach poison or insect growth regulator is then added to the bait. When the ants carry the bait to the nest, the encapsulated poison is gradually released as other workers and queen ants consume it, achieving a slow-release effect and killing the ants. Poison bait requires a small amount of poison, has minimal environmental impact, saves time and effort, and offers effective control, making it suitable for large-scale fire ant control. However, poison baits take a long time to activate, are slow to take effect, and have a significant impact on non-target insects and ecosystems. Using bait in sensitive ecosystems, such as parks and farms, can cause environmental pollution and ecological damage.

[0010] Biological control utilizes predatory and parasitic natural enemies and pathogenic microorganisms to control fire ants. Dragonflies (Pantala flavescens Fabricius) have been found in my country to gather above fire ant nests during their mating flights and prey on the winged ants. A species of mite, the Pulaustoma mite, has also been found in my country to parasitize fire ant workers, causing them to shrink and shorten their lifespans. Microspore bacteria, a pathogenic microorganism, can infect various types of fire ant microsporidia, causing queen ants to lose weight and produce fewer eggs. However, biological control techniques are subject to various factors under natural conditions, making them unstable in their control of fire ants.

[0011] Red imported fire ants are social insects with a high migration frequency: from low-lying areas to high-elevation areas from March to June, from high-humidity areas to shady areas from July to August, and from September to November to areas with abundant food. In November, they migrate to sunny, warm areas. They remain relatively fixed in winter, primarily to stay warm. They migrate rapidly, often moving to the opposite side to build a new nest in a single night. Red imported fire ants also frequently invade food-rich areas such as homes, schools, lawns, and flower beds, increasing their chances of contact with humans. During their migrations, field workers such as farmers and pond fishermen are particularly vulnerable to attack. Therefore, the development of spatial repellents and multi-purpose formulations for red imported fire ants is urgent.

[0012] The prior art of selecting volatile components and their combinations as repellents is as follows:

[0013] The 2021 CN 108366559 B authorization announcement states that benzyl alcohol, 2-phenylethanol, 3-phenylpropanol, and 1-phenyl-2-propanol dissolved in a solvent, along with preservatives, antioxidants, and colorants, can effectively repel vespids without harming honeybees. The compound can quickly neutralize the aggressiveness of vespids and cause them to avoid them, while also exhibiting excellent selectivity against other natural enemies or humans. However, no repellency tests were conducted on red imported fire ants.

[0014] In December 2021, CN 113812417 A disclosed a "sweet potato ant weevil repellent and its use method," which is mainly composed of n-octanol, nerol, and citral. Tween and other surfactants are added to solubilize the compound in an aqueous solution. After soaking the sweet potato for 1 minute, take it out and dry it. It can be used to repel sweet potato ants. It has the advantages of simple preparation, low cost, green and efficient, and no drug resistance. However, there is no research on the repellent effect of the compound on red imported fire ants.

[0015] In March 2022, CN 114617125 A disclosed "the use of 2-methoxy-3-methyl-1,4-benzoquinone in controlling fire ants and Monochamus alternatus." On August 11, 2023, CN 116569921 A disclosed menthyl acetate, also screened and developed from compounds extracted from insects. Menthyl acetate exhibits a strong repellent effect against fire ant workers, sexual females, and males. On August 29, 2023, CN 116649338 A disclosed 2,2-methylenebis[6-(1,1-dimethylethyl)-4-methylphenol] (CAS: 119-47-1) as the active ingredient in a fire ant repellent, primarily used to repel fire ant workers. However, this compound, identified from the defensive chemicals in the venom of wild black-brown ants, is scarce and difficult to obtain. Commercially available varieties are limited and expensive, hindering widespread use. In June 2021, CN113016797 A disclosed "a drug for killing or repelling red imported fire ants", which uses isoeugenol methyl ether as the active ingredient. It has a strong repellent effect on red imported fire ants. It is edible and non-toxic to humans and animals. It is an environmentally friendly repellent. It is green and environmentally friendly, has simple ingredients, weak volatility, long duration, and low cost, which helps to repel red imported fire ants over a large area. In August 2023, CN116649347A disclosed a compound that repels red imported fire ants, mainly butyl anthranilate compounds. The above documents all describe the repellent effect of a single compound and do not involve a reasonable combination of other compounds.

[0016] In January 2020, CN111202099 A disclosed an insect repellent for red imported fire ants (RIFA). It contains a mixture of Vietnamese cinnamon bark essential oil and cinnamyl acetate (0.01% to 10%), an emulsifier (1% to 10%), and the balance (water). In October 2021, CN 113476363A disclosed the use of pyrethrins and azadirachtin as key ingredients for repelling RIFA. In March 2020, CN110859182A disclosed a "Method for Effectively Reducing the Density of RIFA in Green Spaces Using Aromatic Plants," using a combination of two or more of lemongrass, peppermint, spearmint, and mugwort to control RIFA. (The content of small molecule organic compounds emitted by the aromatic plants is: oxidized peppermintone, menthofuran, menthol, menthone, piperonadienone, thymol, α-thujone, geranyl aldehyde, menthone, camphor, isomenthone, 1,8-cineole, β-pinene, (e)-geranic acid). On July 5, 2022, CN 114698638 A disclosed that "the application of hexanoic acid and hexyl hexanoate in repelling red imported fire ants" is greener and more environmentally friendly. The above documents all have 1-2 essential oils or plant ingredients directly mixed, but do not involve the precise chemical structure information of specific compounds.

[0017] In December 2021, CN 111867379 B authorized the publication of "Pest Repellents and Pest Repellent Products". The repellent is mainly based on the world's recognized effective insect repellent ingredient - Icaridin (1-methylpropyl-2-(2-hydroxyethyl) 1-piperidinylcarboxylate), also known as Picaridin. It plays an important role in the prevention and control of highly lethal insect-borne diseases such as malaria, dengue hemorrhagic fever, yellow fever, West Nile virus, Lyme disease, and forest encephalitis. Its forms include spray water, mosquito repellent water, insect repellent wipes and insect repellent sticks, which can provide 2-5 hours of insect repellent protection. However, due to patent protection, Icaridin was registered as a pesticide in my country relatively late, and it is more used in Europe and the United States. The domestic price is relatively expensive, and this is a chemical pesticide mosquito repellent with a clear mechanism of action. The patent document is based on this chemical mosquito repellent ingredient, and some volatile compounds derived from essential oils are added as an auxiliary to play a synergistic role. Summary of the Invention

[0018] The technical problem to be solved by the present invention is to provide a repellent composition for preventing and controlling pests (especially ants) based on different volatility.

[0019] In order to solve the above problems, the present invention provides a pest repellent, which contains the following volatile compounds as pest repellent ingredients:

[0020] a) Aldehydes with repellent activity;

[0021] b) ketones or esters with repellent activity;

[0022] c) linear or branched alkanols and aromatic alcohols, phenols and ethers having repellent activity.

[0023] As an improvement of the pest space repellent of the present invention:

[0024] The mass ratio of aldehydes with repellent activity: ketones or esters with repellent activity: linear or branched alkanols and aromatic alcohols, phenols and ethers with repellent activity is 1:0.1-100:0.1-100.

[0025] The mass ratio of aldehydes with repellent activity: ketones or esters with repellent activity: linear or branched alkanols and aromatic alcohols, phenols and ethers with repellent activity is preferably 1.7-2.4:0.6:2.8-3.2.

[0026] The space repellent (concentrate) is composed of a pest repellent component and a matrix; the mass content of the pest repellent component is preferably 53-60%. The matrix can be, for example, olive oil or dipropylene glycol.

[0027] As a further improvement of the pest space repellent of the present invention:

[0028] The aldehydes having repellent activity are at least any one of the following: paraldehyde, tetraacetaldehyde, five-carbon diacetal, natural aldehyde, undecanal, dodecanal, glutaraldehyde, citronellal, geranyl aldehyde, neral, phenylacetaldehyde, lilyral, cinnamaldehyde, hydroxycitronellal, and anisaldehyde;

[0029] The ketone or ester having repellent activity is at least any one of the following: coumarin (ketone), menthone, citronellyl formate, citronellyl acetate, butyl-decanoic acid lactone, methyl geranate, methyl salicylate, methyl jasmonate, benzyl acetate, agarwood acetate, juniper acetate, geranyl acetate, methyl dihydrojasmonate;

[0030] The linear or branched alkane alcohols and aromatic alcohols, phenols and ethers having repellent activity are at least any one of the following: dipropylene glycol, linalool, pinacol, citronellol, geraniol, dehydrolinalool, menthol, cinnamyl alcohol, sesquiterpenoids, eucalyptol; benzyl alcohol, 1-phenylethanol, 2-phenylethanol, 3-phenyl-1-propanol, 1-phenyl-2-propanol; carvacrol, thymol, eugenol, isoeugenol, isoeugenol methyl ether, guaiacol, 2,6-di-tert-butylphenol; diethylene glycol monomethyl ether, diethylene glycol monoethyl ether (diethylene glycol monoethyl ether), isoeugenol methyl ether.

[0031] Note: Dipropylene glycol, citronellol, geraniol, linalool, etc. are straight-chain or branched alkanols; benzyl alcohol, 1-phenylethanol, 2-phenylethanol, 3-phenyl-1-propanol, 1-phenyl-2-propanol, etc. are straight-chain or branched aromatic alcohols.

[0032] As a further improvement of the pest space repellent of the present invention:

[0033] Aldehydes with repellent activity, ketones or esters with repellent activity, preferably ingredients with repellent activity and simultaneously having antiseptic effects (having antiseptic and bactericidal effects);

[0034] More preferably, diols and / or alcohol ethers having a low saturated vapor pressure are contained.

[0035] As a further improvement of the pest space repellent of the present invention:

[0036] The aldehydes having repellent activity are preferably five-carbon diacetal, glutaraldehyde, and cinnamaldehyde;

[0037] The linear or branched alkanols and aromatic alcohols, phenols and ethers having repellent activity are preferably diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, isoeugenol methyl ether, carvacrol and thymol.

[0038] The present invention also provides the use of the above-mentioned pest space repellent: for repelling pests and ants, especially red imported fire ants.

[0039] As an improvement to the use of the pest space repellent of the present invention: the pest repellent product is in the form of a liquid preparation, gel preparation or ointment preparation of the repellent, which can be used as a space repellent, sprayed or applied on clothing, shoes, etc. to quickly repel pest attacks, or loaded in controlled-release materials such as gels, pastes, microcapsules, microspheres, etc. to provide long-lasting prevention and control of pest attacks.

[0040] During the invention process, the present invention fully considered the following problems to be solved:

[0041] Existing patents, some of which focus on single volatile compounds, others on mixtures of crude essential oil extracts, and still others on single, expensive compounds, only provide short-term repellent activity, with varying degrees of effectiveness and no data on the duration of repellent effect. Only one patent mentions a 2-5 hour duration of repellent activity against red imported fire ants, but this relies primarily on the long-lasting effect of the chemical insecticide repellent icaridin, combined with a supplementary volatile compound for synergistic effect.

[0042] Therefore, there has been no report on a more ideal formula composition of volatile active components derived from essential oils, which has a fast-acting and long-lasting effect, can be widely sourced, easily obtained, derived from essences or foods, reasonably priced, does not contain chemical pesticides, is environmentally friendly and degradable, and can be made into different preparation forms such as sprays, coatings, and gel sustained release.

[0043] In order to solve the above problems, the research group of the inventor conducted in-depth research. Based on the performance principle of rapid repellence and long-lasting repellence of reasonable combinations of volatile compounds, the research results found that: some volatile substances with simple structures are reasonably matched and combined, and according to the different volatilization rates (or saturated vapor pressures) of the repelling volatile components, highly volatile and less volatile components are selected and matched in a certain proportion. This can quickly take away the aggressiveness of red fire ants and make them repel, and will not cause harm to other natural enemies. While having fast-acting activity, it also takes into account a certain long-lasting effect. It is economical and environmentally friendly, and can be made into spray preparations, smear preparations, etc. according to different application scenarios, thereby completing the present invention.

[0044] The present invention provides the following solutions to the above problems:

[0045] By screening volatile substances with repellent activity against pest ants, especially red imported fire ants (including aldehydes with repellent activity, ketones or esters with repellent activity), reasonable combinations are made according to the size of the repellent activity and the different volatilization rates of the volatile substances, that is, the size of the saturated vapor pressure, and then the appropriate proportions are screened to prepare a formula composition. The various components have fast-acting and long-lasting synergistic effects, and auxiliary antibacterial and preservative compounds and other auxiliary ingredients are added.

[0046] The volatilization speed of various components, that is, the volatilization rate, is different, which is directly related to its vapor pressure. The specific saturated vapor pressure of various volatile components is as follows:

[0047] 1,3-Propanediol (0.08 mm Hg (20°C)), Glycerol (<1 mm Hg (20°C)), Dipropylene glycol (<0.01 mm Hg (20°C)), Geraniol (Leaf alcohol) (0.013 mmHg at 25°C), Tripropylene glycol (<0.01 mm Hg (25°C)), Diethylene glycol (0.01 mm Hg (20°C)), Triethylene glycol (<0.01 mm Hg (20°C)), 1,3-Butanediol (0.06 mm Hg (20°C)), 1,5-Pentanediol (0.007 mm Hg (25°C)), 1,6-Hexanediol (0.0667 mm Hg (25°C)), Isoamyl alcohol (2 mm Hg (20°C)), Benzyl alcohol (0.225 mm Hg (25°C)), Phenylethyl alcohol (0.1 ± 0.5 mmHg at 25°C) 25℃), 3-phenylpropanol (0.0±0.5 mmHg (25℃)), 1-phenyl-2-propanol (0.067 mmHg at 25℃), linalool (0.17 mm Hg (25℃)), geraniol (0.013 mmHg at 25℃), linalool oxide (0.00169 mmHg at 25℃), isopentanol (2 mm Hg (20℃)), cinnamyl alcohol (<0.01 mm Hg (25℃)), menthol (0.8 mm Hg (20℃)), citronellol (~0.02 mm Hg (25℃)), terpineol (0.021 mmHg (20℃)), β-caryophyllene 0.025 mmHg (20℃), α-pinene (3.49 mmHg at 25℃), β-pinene (3.49 mmHg at 25℃), methylparaben (0.014 mmHg at 25℃), methylparaben (0.014 mmHg at 25℃), methylparaben (0.014 mmHg at 25℃), methylparaben (0.014 mmHg at 25℃), methylparaben (0.014 mmHg at 25℃), methylparaben (0.014 mmHg at 25℃), methylparaben (0.014 mmHg at 25℃) 25℃), limonene (1.54 mmHg at 25℃), methyl salicylate (0.0296 mmHg at 25℃), butyl decalactone (0.0047 mmHg at 25℃), benzaldehyde (3.82 mm Hg (25℃)), phenylacetaldehyde (0.368 mmHg at 25℃), isovaleraldehyde (49.3 mmHg at 25℃), cinnamaldehyde (0.0265 mmHg at 25℃), trans-cinnamaldehyde (0.0265 mmHg at 25℃), α-hexylcinnamaldehyde (0+0.6 mmHg at 25℃), hexanal (10 mm Hg (20℃)), heptaldehyde (0.3 mmHg at 25℃), octanal (2 mm Hg (20℃)), nonanal (0.26 mmHg at 25℃), Hg (25℃)), hydroxycitronellal (0.00318mmHg at 25℃), anisaldehyde (0.0249mmHg at 25℃), paraldehyde (0.0544mmHg at 25℃), tetraacetaldehyde (0.0495mm Hg at 25℃)), diethylene glycol dimethyl ether (3.2mmHg at 25℃), diethylene glycol monomethyl ether (0.016mmHg at 25℃), diethylene glycol monoethyl ether (0.000386mmHg at 25℃), isoeugenol methyl ether (0.011mmHg at 25℃), benzyl acetate (0.164mmHg at 25℃), methyl jasmonate (23mm Hg(110℃)), methyl dihydrojasmonate (0.0016mmHg at 25℃), phenol (0.0±0.6mmHg at 25℃), 2,6-di-tert-butylphenol (<0.01mm Hg(20℃)), carvacrol (0.023-0.05mmHg at 25℃), thymol (thymol, 0.0376mmHg at 25℃).

[0048] In the present invention:

[0049] Different compounds derived from plant essential oils (EOS), particularly aldehydes, ketones, or esters with repellent activity, linear or branched alkanols and aromatic alcohols, phenols, and ether alcohols, can work synergistically to enhance their potential and efficacy. Based on varying volatility and potency, effective combinations can ensure rapid effectiveness while enhancing long-lasting efficacy. Spray or apply to clothing, shoes, or place in gels or pastes for controlled-release materials near ponds for long-term control.

[0050] The present invention has the following beneficial effects:

[0051] According to the composition formula of the present invention, the repellency rate can reach more than 98% in half an hour, and the repellency time in the wild can reach 8-9 hours depending on the concentration. It can be used as a spatial repellent to quickly repel pests, especially the harm of foreign invasive red fire ants, or to repel pest invasions in small areas with a lasting effect. The pest repellent does not substantially contain chemical pesticide insecticide components. In summary, the present invention discloses a volatile composition for preventing and controlling red fire ants and its use, which is mainly composed of volatiles with repellent activity in natural habitats according to different effective combinations of volatilization rates. The present invention studies the repellent effect of volatiles on red fire ants from indoor repellent and field repellent tests, and screens different composition formulas. The formula has a good repellent effect on red fire ants through indoor and field test, and can be used for the repellent effect of red fire ants in the wild. The above-mentioned compounded and optimized mixture can not only have a high preventive effect on the repellent effect of red fire ants and a long lasting effect, but also has the advantages of simple preparation, low cost, green and efficient, no drug resistance, environmental friendliness, human and animal safety, and natural environmental protection. DETAILED DESCRIPTION

[0052] The present invention is further described below with reference to specific embodiments, but the protection scope of the present invention is not limited thereto:

[0053] Hereinafter, the present invention will be described in more detail by giving examples. The present invention is not limited to the following examples and can be appropriately modified within the scope of the gist of the preceding and following texts. All of these modifications are included in the technical scope of the present invention.

[0054] The pest repellent of the present invention was prepared using fire ants as test subjects to test the effectiveness of the examples. In the following examples, although the weight units of each component are expressed in g, they can be reduced or increased in proportion to any multiple. The weight unit "g" can be regarded as "weight ratio %".

[0055] Preparation of pest repellent:

[0056] According to the compositions shown in Table 1 below, the pest repellents of Examples 1 to 9 and Comparative Examples 1 to 4 were prepared.

[0057] According to the different activities and volatilization rates of various repellents, the following formula is composed:

[0058] Table 1:

[0059]

[0060]

[0061]

[0062] Note: The figures in Table 1 above can also be converted into corresponding percentages, %.

[0063] The preparation method of the above-mentioned pest repellent is as follows: in a sealed container, the above-mentioned compounds are weighed and added respectively, and finally supplemented with matrix oils, mixed evenly, and sealed for later use.

[0064] During the invention process, the following comparative cases were also conducted:

[0065] Comparative Example 5-1: The use of "0.2 g of metaldehyde" was cancelled, and the amount of "neral" was increased accordingly, that is, the amount of "neral" was changed from 1.8 g to 2 g. The rest was the same as in Example 7.

[0066] Comparative Example 5-2: The "tetraacetaldehyde" in Example 7 was replaced with "triacetaldehyde", and the dosage remained unchanged at 0.2 g. The rest was the same as in Example 7.

[0067] Comparative Example 5-3: The use of "0.6 g of citronellyl formate" was cancelled, and the amount of "eucalyptol" was increased accordingly, that is, the amount of "eucalyptol" was changed from 1.0 g to 1.6 g, and the rest was the same as Example 7.

[0068] Comparative Example 5-4: The "citronellyl formate" in Example 7 was replaced with "methyl salicylate", and the dosage remained unchanged at 0.6 g. The rest was the same as in Example 7.

[0069] Comparative Example 6-1: The use of "0.2 g of metaldehyde" was cancelled, and the amount of "cinnamaldehyde" was increased accordingly, that is, the amount of "cinnamaldehyde" was changed from 1.5 g to 1.7 g. The rest was the same as in Example 2.

[0070] Comparative Example 6-2: The "tetraacetaldehyde" in Example 2 was replaced with "triacetaldehyde", and the dosage remained unchanged at 0.2 g. The rest was the same as in Example 2.

[0071] Comparative Example 6-3: The use of "0.6 g of citronellyl formate" was cancelled, and the amount of "2-phenylethanol" was increased accordingly, that is, the amount of "2-phenylethanol" was changed from 1.0 g to 1.6 g. The rest was the same as in Example 2.

[0072] Comparative Example 6-4: The "citronellyl formate" in Example 2 was replaced with "coumarin", and the dosage remained unchanged at 0.6 g. The rest was the same as in Example 2.

[0073] Indoor avoidance effect experiment:

[0074] Test agent:

[0075] The corresponding repellents were prepared according to the above embodiments and comparative examples.

[0076] Test method:

[0077] 1. Prepare a diluted solution of the insect repellent by dissolving 50 mg of each liquid repellent (the concentrate prepared in the above case) in 5 ml of acetone.

[0078] 2. Use a sprayer to apply 0.5 ml of the test solution to one half of a 15 cm diameter filter paper and air dry. In this case, each half of the dried filter paper contains 5.0 mg of the drug.

[0079] 3. Then, the filter paper was placed in a 15 cm diameter glass petri dish (about 1.5 cm high) so that the half of the filter paper containing the insect repellent (the treated area) and the half of the filter paper not containing the insect repellent (the untreated area) did not overlap. The inner wall of the glass petri dish was then coated with talcum powder.

[0080] 4. Place 30 red imported fire ants as test insects in each area, and count the number of red imported fire ants (insect count) in the treated area and the untreated area after 5 minutes, 2 hours, and 6 hours.

[0081] 5. Perform the experiment three times and take the average value to calculate the approach-avoidance rate;

[0082] 6. Calculate the pest avoidance rate according to the following formula:

[0083] Avoidance rate (%) = (number of insects in the untreated area - number of insects in the treated area) / number of test insects × 100

[0084] Avoidance rate at different times in indoor avoidance experiment

[0085]

[0086]

[0087] From the repellent test, it can be seen that the examples of the formula of the present invention all have certain repellent activity, especially Examples 2 and 7, which have the best repellent effect. The repellent effect on red imported fire ants is high in a short period of time (up to 100% in 5 minutes), and the long-lasting effect is also relatively good, with a repellent rate of about 80% after 6 hours.

[0088] Sprayable preparations (liquid preparations)

[0089] The liquid preparation of the repellent can be composed of the following formula of Example 7: 1.0 g of eucalyptol, 1.8 g of neral, 0.2 g of metaldehyde, 0.6 g of citronellal, 0.8 g of carvacrol, 1.2 g of isoeugenol methyl ether, dipropylene glycol is added to 10 g, 75% ethanol is added to make the volume 100 ml, and the mixture is placed in a conventional spray plastic bottle. Before the pond in the south (where red imported fire ants are reported to frequently appear), carefully spray it on clothes at a rate of about 15 mL / m 2 The farmer worked for about six hours near the pond and field without suffering from red fire ants or bites from other pests, especially spraying the cuffs and lower half of the pants.

[0090] Loaded sustained-release spray preparations

[0091] As a pest repellent ingredient, the product of Example 7 was prepared and used according to the following loading scheme:

[0092] 1) 1 ml of the concentrate prepared according to the ratio of Example 7 was directly adsorbed onto 1 cm × 1 cm highly absorbent sponge blocks. The blocks were then placed in areas where red imported fire ants were present, with one block placed every 3 meters. Farmers working near a pond could work in the pond or field for approximately 6-7 hours without being bitten by red imported fire ants or suffering from the attachment or bites of other pests.

[0093] 2) Weigh 2 grams of branched polyethyleneimine (molecular weight 10K) and dissolve it in 10 ml of ethanol solution, mix well, and directly add 10 grams of the concentrate prepared according to Example 7, mix well, and spray directly for use; alternatively, take 2 grams of the mixed liquid and directly adsorb it onto a 1.5 cm × 1.5 cm sponge cube and place it in an area where red imported fire ants come and go, with one cube placed every 3 meters. Farmers working by the pond can work in the pond field for about 6-7 hours without being bitten by red imported fire ants or being bothered by the attachment or bites of other pests.

[0094] Finally, it should be noted that the above examples are merely specific embodiments of the present invention. Obviously, the present invention is not limited to the above examples and is subject to numerous variations. All variations that can be directly derived or conceived by a person of ordinary skill in the art from the disclosure of the present invention are considered to be within the scope of protection of the present invention.

Claims

1. A pest repellent, characterized in that: The pest repellent ingredients of the pest space repellent are composed of eucalyptol, neral, metaldehyde, citronellyl formate, carvacrol and isoeugenol methyl ether in a mass ratio of 1.0:1.8:0.2:0.6:0.8:1.

2.

2. A pest repellent, characterized in that: The pest repellent component of the pest space repellent consists of 2-phenylethanol, cinnamaldehyde, metaldehyde, citronellyl formate, eugenol and diethylene glycol monoethyl ether in a mass ratio of 1.0:1.5:0.2:0.6:0.8:1.

2.

3. The use of the pest space repellent according to claim 1 or 2, characterized in that: Used to repel ant pests.

4. The use of the pest space repellent according to claim 3, characterized in that: The ant pests are fire ants.

5. The use of the pest space repellent according to claim 3 or 4, characterized in that: The pest repellent preparation is in the form of a liquid preparation, a gel preparation or an ointment preparation.

6. The use of the pest space repellent according to claim 5, characterized in that: The pest repellent preparation is loaded on gel, gel paste, microcapsule, or microsphere controlled-release material.

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