The invention provides a nervonic acid composition and application thereof in neuroprotection, and belongs to the field of medicines. By organically compounding nervonic acid, phosphatidylserine, DHA,celery seed oil, Chinese herb extracts and nutrient supplements according to an assistant and guide principle, the nervonic acid composition integrates a nerve cell nutrient, a nerve fiber repair agent, a neurotransmitter activator, a nerve energy security agent and other mechanisms, so that the permeability of a nerve cell membrane is improved by using multiple systems and multiple ways, the release of a neurotransmitter is promoted, the conduction of nerve impulse is accelerated, a nerve information passage is repaired and dredged, and the brain function is activated or reversed. The nervonic acid composition breaks through the limit and the defect of a certain component which is singly applied, and becomes an important product for preventing and treating encephalopathy. Experiments onmore than 3,000 volunteers by trial products prove that the nervonic acid composition has a comprehensive health-care effect on the nervous system, and particularly has a satisfactory effect of preventing and reversing physical disability, language barrier and mental disorder of patients suffering from cerebral apoplexy.