Long-acting solenopsis invicta killing bait and preparation method thereof

Abstract

The invention relates to the field of solenopsis invicta prevention and control, in particular to a long-acting solenopsis invicta killing bait and a preparation method thereof.The long-acting solenopsis invicta killing bait is prepared from indoxacarb, sophocarpidine, huperzine A, piperidine, modified sodium alginate, corn, wheat, fructose, potassium sorbate, calcium propionate, vitamin E and modified glycerin. The insecticide components are prepared into microcapsules which wrap insecticidal active components and have a slow release effect, and the huperzine A inhibits acetylcholin esterase, increases the concentration of acetylcholine and activates a sodium ion channel; indoxacarb blocks a sodium ion channel and inhibits nerve impulse; the matrine inhibits Na < + >-ATPase and influences the membrane potential; the effects result in that nerve cells are more susceptible to indoxacarb. The modified sodium alginate changes the permeability of cell membranes and affects ion balance. Various components have a synergistic effect, so that the neurotoxicity is enhanced, the solenopsis invicta paralysis or death is finally caused, and the termite killing effect is improved. The long-acting bait combines efficient insect killing and environment friendliness, and an innovative solution is provided for prevention and control of the solenopsis invicta.

Description

Technical Field

[0001] The invention relates to the field of red fire ant prevention and control, and in particular to a long-acting bait for killing red fire ants and a preparation method thereof. Background Art

[0002] Red fire ants are highly aggressive and highly adaptable invasive pests. They not only cause serious damage to agricultural production, reducing crop yields and quality, but also cause pain to humans and animals due to their aggressiveness. Their bites may cause severe pain, redness, swelling, and even anaphylactic shock. In addition, red fire ants can also destroy the ecological structure of the soil, affect biodiversity, and threaten the survival of native species. According to research, the foraging activities of red fire ant workers begin within a certain temperature range and stop at temperatures that are too high or too low. Specifically, when the soil temperature reaches 19°C, the foraging activities of red fire ant workers begin, and when the soil temperature is 15-43°C, the workers begin to forage, and the maximum foraging rate occurs between 22 and 36°C.

[0003] At present, commonly used methods of killing ants include chemical pesticide spraying, physical methods and traditional baits, but these methods have many shortcomings. Chemical pesticides may have negative impacts on the environment and non-target organisms, physical methods such as irrigation and nest digging have limited effects and are difficult to completely eradicate ant damage, and biological control introduces natural enemies, which may bring new ecological risks, and the effect is unstable and greatly affected by environmental factors.

[0004] The reason why we choose to use baits to kill red fire ants is that they can use the natural feeding behavior of red fire ants to accurately and efficiently deliver toxicity and reduce pollution to the environment. The use of baits can reduce the frequency and amount of chemical pesticides, reduce the health risks to operators, and improve the durability of the prevention and control effect. The stability and effectiveness of traditional baits are easily affected by environmental factors such as rain and temperature changes, which reduces their stability and effectiveness.

[0005] In summary, the development of a temperature-sensitive long-acting bait for killing red fire ants can not only effectively control the harm of red fire ants, but also improve the environmental protection and sustainability of prevention and control measures. The release temperature range of the microcapsules in the bait for killing red fire ants of the present invention is 25 to 35°C. This temperature range just covers the temperature range where red fire ants are most active, ensuring the high efficiency of the bait. By precisely controlling the release of active ingredients, the bait can provide the greatest killing effect during the most active period of red fire ants while reducing the potential impact on the surrounding environment. This temperature-sensitive design not only improves the targeting of the bait, but also helps to extend its validity period, thereby achieving more economical and efficient prevention and control of red fire ants. Summary of the invention

[0006] (1) Technical issues to be solved

[0007] The purpose of the present invention is to provide a long-acting bait for killing red fire ants and a preparation method thereof, so as to solve the problems in the prior art of short-lasting control effect of red fire ants, great environmental impact, complicated operation, etc., and realize efficient, environmentally friendly, simple and long-term control of red fire ants.

[0008] (2) Technical solution

[0009] To achieve the above object, on the one hand, the present invention provides a long-acting bait for killing red fire ants, which is composed of the following raw materials in parts by weight: 4 to 9 parts of insecticide, 15 to 25 parts of attractant, 5 to 10 parts of potassium sorbate, 5 to 10 parts of calcium propionate, 2 to 6 parts of vitamin E, and 5 to 7 parts of modified glycerol;

[0010] The insecticide is the following raw materials in parts by weight: 0.1-1 parts of indoxacarb, 1-2 parts of matrine, 2-3 parts of huperzine A and 1-3 parts of piperidine;

[0011] The attractant is made of the following raw materials in parts by weight: 5 to 8 parts of corn, 5 to 8 parts of wheat and 5 to 8 parts of fructose.

[0012] Furthermore, the insecticide component further comprises, by weight: 2 to 4 parts of modified sodium alginate;

[0013] The modified sodium alginate is generated by graft copolymerization of sodium alginate and poly(N,N-diethylacrylamide), and has vinyl groups and diethylamino groups on the surface.

[0014] Furthermore, the preparation method of the modified glycerol comprises:

[0015] S11. Place a three-necked round-bottom flask in an ice bath, install a constant pressure dropping funnel, a thermometer and a nitrogen conduit, and ensure that the device is well sealed;

[0016] S12. Slowly add glycerol and anhydrous dichloromethane into a three-necked flask, and turn on magnetic stirring to allow the glycerol to be fully dissolved;

[0017] S13. Add sulfur trioxide - dimethylformamide complex and water dichloromethane to the constant pressure dropping funnel and mix them thoroughly;

[0018] S14. Turn on nitrogen protection, place the reaction system in an inert atmosphere, control the reaction temperature between 0 and 5°C, slowly drop the sulfur trioxide-dimethylformamide complex solution into the glycerol solution, the dropwise addition time is about 1 to 1.5 hours, and after the dropwise addition is completed, continue to stir the reaction at 0 to 5°C for 2 to 3 hours;

[0019] S15. The reaction mixture was slowly poured into ice water, stirred for 10 to 15 min, the organic layer and the aqueous layer were separated by a separatory funnel, and the organic layer was dried over anhydrous magnesium sulfate;

[0020] S16. Filter and remove the desiccant, remove the solvent using a rotary evaporator, and the resulting crude product can be further purified by recrystallization or column chromatography.

[0021] Furthermore, the preparation method of the modified sodium alginate comprises:

[0022] S21. Sodium alginate and deionized water were added to the reaction flask, dissolved by electromagnetic stirring, nitrogen was passed through for 15 min, and ammonium persulfate, N,N-diethylacrylamide monomer, and N,N'-methylenebisacrylamide were added in sequence;

[0023] S22. Close the reaction flask, introduce nitrogen, heat the reaction flask to 40-60°C, react for 4-6h, and maintain nitrogen protection and stirring;

[0024] S23. After the reaction is completed, the reaction solution is cooled, the grafted polymer is separated by n-hexane precipitation and vacuum dried to constant weight to obtain a crude product;

[0025] S24. The crude product was extracted with acetone in a Soxhlet extractor for 24 h to remove the ungrafted homopolymer and monomer, and then vacuum dried to a constant weight to obtain a grafted copolymer.

[0026] Furthermore, the insecticide component is prepared into a microcapsule encapsulating the insecticide active ingredient with a sustained-release effect, and the preparation method includes:

[0027] S31. Dissolve the modified sodium alginate in deionized water and stir to prepare a gel solution;

[0028] S32. Indoxacarb, matrine, huperzine A and piperidine are dissolved in ethanol, stirred and mixed, and then slowly added to the glue to form an oil-water emulsion, and the solution is dispersed into tiny droplets by a spray device or a dropping device;

[0029] S33. Under stirring conditions, the formed droplets were injected into a 1.5% CaCl2 solution. As the cross-linking reaction proceeded, the droplets gradually gelled to form microcapsules with a certain mechanical strength.

[0030] S34. The formed microcapsules are separated from the reaction system by filtration or centrifugation, and then washed with deionized water to remove possible residual crosslinking agent and other impurities;

[0031] S35. The washed microcapsules are vacuum dried to remove residual moisture;

[0032] On the other hand, based on the same inventive concept, the present invention also provides a method for preparing a long-acting bait for killing red fire ants, which is applied to the long-acting bait for killing red fire ants, comprising the following steps:

[0033] S41. Preparation of modified sodium alginate;

[0034] S42. Preparation of insecticide component microcapsules;

[0035] S43. Preparation of modified glycerol;

[0036] S44. The corn, wheat and fructose are mixed uniformly according to the proportion, and then potassium sorbate, calcium propionate, modified glycerol and vitamin E are added to the mixed carrier material;

[0037] S45. The cured microcapsules were mixed with corn, wheat, fructose, potassium sorbate, calcium propionate and vitamin E in proportion, and modified glycerol was added to enhance wettability and adhesion;

[0038] S46. After the mixture is uniformly mixed in a blender, it can be formed by extrusion or die, and dried to remove moisture to obtain a long-lasting bait for killing red fire ants.

[0039] The mechanism of action of the above raw material components is as follows:

[0040] Indoxacarb is a highly effective, low-toxic oxadiazine insecticide with a unique mechanism of action and a broad insecticide spectrum. It is metabolized into active DCJW (N-demethoxycarbonyl) in the body of red fire ants, which irreversibly binds to the inactivated sodium ion channels of red fire ant neurons, thereby inhibiting the transmission of nerve impulses and causing paralysis and death of red fire ants.

[0041] Matrine, a plant-based pesticide, is isolated from the roots, stems, leaves and flowers of Sophora flavescens. It has contact and stomach poison effects, and also has certain antibacterial effects on fungi and bacteria. Its insecticidal mechanism is mainly through paralyzing the nerve center of the red fire ant, causing the protein coagulation and stomata blockage of the red fire ant, and eventually suffocating the red fire ant to death.

[0042] Huperzine A is an alkaloid extracted from the Huperzine plant Huperzineaceae. It is a potent and reversible acetylcholinesterase inhibitor. Its main mechanism of action on red fire ants is its neurotoxicity, which can interfere with nerve conduction and affect the normal function of neurons.

[0043] Piperidine is a synergist that can increase the toxicity of the active ingredients indoxacarb and matrine in the bait, thereby improving the killing effect on red fire ants. In addition, piperidine may further improve the attractiveness and insecticidal efficiency of the bait by improving the physical properties of the bait or affecting the behavior of red fire ants as a signal molecule.

[0044] Modified sodium alginate is used as the main wall material of the microcapsule to encapsulate indoxacarb, matrine, huperzine A and piperidine, control the release rate of these active ingredients, improve the long-term effect and environmental stability of the bait, and contain temperature-sensitive materials to enable the bait to release active ingredients within the temperature range where red fire ants are active, thereby improving the insecticidal efficiency.

[0045] Corn and wheat are carriers of baits, which have a good attractant effect on red fire ants due to their rich carbohydrates and suitable nutrients. Adding corn and wheat to the bait can not only attract red fire ants to come and feed, but also serve as a long-term energy source to maintain the activity of red fire ants, thereby improving the prevention and control effect of the bait.

[0046] Fructose is a sweetener that uses its sweetness to significantly increase the attraction and feeding rate of baits to red fire ants. At the same time, as a quick energy source, it promotes red fire ants to bring baits back to the nest, increases the spread efficiency of the bait, and ensures that the insecticide ingredients can be effectively delivered to the entire ant colony, thereby improving the ant killing effect and long-term effectiveness of the bait. In addition, fructose can also improve the physical properties of the bait, enhance its stability and wettability under different environmental conditions, and provide an environmentally friendly and efficient solution for red fire ant control.

[0047] Potassium sorbate is a preservative that effectively extends the shelf life of the bait and maintains its stability. It inhibits the growth of microorganisms and reduces the risk of spoilage during storage and transportation, ensuring that the bait still has the expected insecticidal effect and safety when applied. At the same time, the addition of potassium sorbate also helps maintain the activity of other ingredients in the bait, thereby maintaining the efficiency and reliability of the bait throughout its entire use cycle.

[0048] Calcium propionate is a preservative that effectively inhibits the growth of microorganisms in baits with its antibacterial properties, thereby extending the shelf life of the bait and maintaining its stability. In addition, the slow release properties of calcium propionate help to provide continuous protection during the use of the bait, reduce bait degradation caused by microbial activity, and ensure the durability of the bait in outdoor applications. At the same time, as a food-grade additive, the safety of calcium propionate also makes it suitable for environmentally friendly and low-toxic pest management strategies.

[0049] Vitamin E is an antioxidant that protects the active ingredients in the bait from oxidative damage by capturing free radicals and preventing oxidation reactions, thereby extending the shelf life of the bait and maintaining its insecticidal effect. In addition, the addition of vitamin E helps to stabilize the overall formula of the bait, improve its chemical stability under different environmental conditions, and ensure that the bait maintains high efficiency and reliability during storage and use.

[0050] As a wetting agent, modified glycerin improves the wettability of the bait, promotes the uniform distribution of the bait in the soil and the feeding efficiency of red fire ants; as a viscosity regulator, it improves the physical properties of the bait, and increases its adhesion and dispersibility during the application process; at the same time, modified glycerin also has the function of a stabilizer, which helps to maintain the stability of the active ingredients in the bait, reduce the impact of environmental factors on the effectiveness of the bait, and ensure long-term and effective prevention and control of red fire ants.

[0051] (3) Beneficial effects

[0052] Compared with the prior art, the present invention has the following beneficial effects:

[0053] 1. The long-acting bait prepared by modified sodium alginate microcapsule technology can achieve controlled release within the temperature range where red fire ants are active, improving the targeting and efficiency of the bait. This temperature-sensitive release mechanism ensures the effective release of active ingredients at suitable temperatures and reduces ineffective release at unsuitable temperatures, thereby improving the overall prevention and control effect while reducing potential impacts on the environment.

[0054] 2. Huperzine A inhibits the activity of acetylcholinesterase, indoxacarb is a sodium channel blocker, and matrine is a plant-derived insecticide. The three work synergistically to improve the insecticidal efficiency of red fire ant baits and achieve faster prevention and control effects.

[0055] 3. By optimizing the formula, the bait of the present invention reduces the production and use costs while ensuring the effect, and is easier to apply and operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 The microcapsule inclusions in Example 1 under a microscope. DETAILED DESCRIPTION

[0057] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0058] The test equipment and preparations of the examples described below are as follows:

[0059] Electronic balance (Sartorius, Germany), electric blast constant temperature dryer (Shanghai Formosa Experimental Equipment), stainless steel reactor (Shanghai Laibei), electric constant temperature water bath (Jiangsu Kedao), rotary evaporator (Shanghai Darlow Scientific Instruments), pulverizer (Shandong Tianfang Machinery), magnetic stirrer (Shanghai Meiyingpu), Soxhelt extractor (Qingdao Juchuang); sodium alginate was purchased from Anhui Zhonghong Bioengineering Co., Ltd., matrine was purchased from Shanghai Milkway Supply Chain Management Co., Ltd., indoxacarb was purchased from Shanghai MacLean Biochemical Technology Co., Ltd., huperzine was purchased from Xi'an Weisbo Biotechnology Co., Ltd., and chemicals and reagents were purchased from Sigma-Aldrich.

[0060] Example 1

[0061] This embodiment discloses a long-lasting bait for killing red fire ants, which is composed of the following raw materials by weight: 6 parts of insecticide, 16 parts of attractant, 6 parts of potassium sorbate, 7 parts of calcium propionate, 3 parts of vitamin E, and 6 parts of modified glycerol; the insecticide is the following raw materials by weight: 0.2 parts of indoxacarb, 1.5 parts of matrine, 2.5 parts of huperzine A, and 1.8 parts of piperidine; the attractant is the following raw materials by weight: 6 parts of corn, 7 parts of wheat, and 6 parts of fructose. The insecticide components, by weight, also include: 3 parts of modified sodium alginate; the modified sodium alginate is generated by graft copolymerization of sodium alginate and poly (N, N-diethyl acrylamide), and has vinyl groups and diethylamino groups on the surface.

[0062] In the bait for killing red fire ants, Huperzine A can inhibit the activity of acetylcholinesterase, thereby increasing the concentration of acetylcholine. Acetylcholine will bind to the receptors on the postsynaptic membrane, causing changes in the post-membrane potential, activating the sodium ion channel, and further transmitting the signal. When the concentration of acetylcholine in the synaptic cleft increases, the activation frequency of the sodium ion channel and the transmission efficiency of the nerve signal will increase. Indoxacarb, as a sodium ion channel blocker, blocks the flow of sodium ions, thereby inhibiting the transmission of nerve impulses. When the activation frequency of the sodium ion channel increases, it will provide more opportunities for indoxacarb to bind to the sodium ion channel, and the efficiency of nerve signal transmission will increase. The increase will cause more sodium ion channels to be in an open state, so the number of sodium ion channels blocked by indoxacarb will increase, thereby improving the insecticidal effect of indoxacarb. At the same time, due to the activation frequency of sodium ion channels, sodium ions continue to flow into the cell, and matrine has an inhibitory effect on Na+-ATPase. Since Na+-ATPase is inhibited, cells cannot effectively pump excess Na+ out of the cell. Changes in the sodium ion concentration gradient inside and outside the cell may cause the resting membrane potential of the cell to become more negative. If the resting membrane potential becomes more negative, the activation threshold of the sodium ion channel may be reduced, which further makes the channel easier to activate.

[0063] Excess acetylcholine can bind to more receptors, thereby activating certain types of acetylcholine receptors, such as nicotinic acetylcholine receptors (nAChRs), leading to the opening of calcium ion channels. The opening of calcium ion channels increases intracellular Ca 2 + concentration, the mechanism of action of matrine is multifaceted, it can change the release and reception of neurotransmitters, thereby affecting the behavior and physiological functions of red fire ants, and can also affect the calcium ion channels in the red fire ant nervous system, thereby affecting intracellular Ca 2 + concentration, because intracellular Ca 2+The change in concentration breaks the ion balance of the nervous system. The destruction of this balance not only directly affects the transmission of nerve signals, but also makes nerve cells more fragile and more susceptible to other neurotoxins, putting nerve cells in a "sub-healthy" state. Nerve cells become more susceptible to the effects of indoxacarb, and the effect of indoxacarb becomes more significant, further enhancing its effect of blocking sodium ion channels. The obstruction of sodium ion flow causes nerve cells to be unable to generate and transmit action potentials, the transmission of nerve impulses is interrupted, and the nervous system of red fire ants gradually loses function, eventually leading to paralysis symptoms in red fire ants, which can be fatal in severe cases.

[0064] The thermosensitivity and biocompatibility of modified sodium alginate enable them to interact with nerve cells, leading to an increase in the fluidity of the cell membrane, thereby changing the permeability of the cell membrane and allowing Na+ and Ca to flow in and out of the cell membrane. 2 + and other small molecules can more easily pass through the cell membrane, affecting the balance of ion concentration inside and outside the cell, and then interfering with the transmission of nerve signals, thereby performing the above-mentioned synergistic effect with huperzine A, indoxacarb and matrine, further improving the ant-killing effect of the red fire ant bait.

[0065] In order to improve the bioavailability of huperzine A, indoxacarb and matrine and prolong their duration of action, modified sodium alginate is used to form a microcapsule structure to encapsulate these three active ingredients. The design of the microcapsule structure allows precise control of the release rate of huperzine A, indoxacarb and matrine to achieve slow release. This slow release method not only improves the bioavailability of huperzine A, indoxacarb and matrine, but also prolongs their duration of action, keeping the red fire ant nervous system in a disturbed or unbalanced state for a long time, thereby achieving a long-lasting insecticidal effect.

[0066] In summary, the development of this long-lasting bait for killing red fire ants not only improves the prevention and control efficiency of red fire ants, but also reduces the use of chemical pesticides, which is of great significance to environmental protection and sustainable development.

[0067] The preparation method of the modified glycerol comprises:

[0068] S11. Place a three-necked round-bottom flask in an ice bath, install a constant pressure dropping funnel, a thermometer and a nitrogen conduit, and ensure that the device is well sealed;

[0069] S12. Slowly add glycerol and anhydrous dichloromethane into a three-necked flask, and turn on magnetic stirring to allow the glycerol to be fully dissolved;

[0070] S13. Add sulfur trioxide - dimethylformamide complex and water dichloromethane to the constant pressure dropping funnel and mix them thoroughly;

[0071] S14. Turn on nitrogen protection, place the reaction system in an inert atmosphere, control the reaction temperature between 0 and 5°C, slowly drop the sulfur trioxide-dimethylformamide complex solution into the glycerol solution, the dropwise addition time is about 1 to 1.5 hours, and after the dropwise addition is completed, continue to stir the reaction at 0 to 5°C for 2 to 3 hours;

[0072] S15. The reaction mixture was slowly poured into ice water, stirred for 10 to 15 min, the organic layer and the aqueous layer were separated by a separatory funnel, and the organic layer was dried over anhydrous magnesium sulfate;

[0073] S16. Filter and remove the desiccant, remove the solvent using a rotary evaporator, and the resulting crude product can be further purified by recrystallization or column chromatography.

[0074] Furthermore, the preparation method of the modified sodium alginate comprises:

[0075] S21. Sodium alginate and deionized water were added to the reaction flask, dissolved by electromagnetic stirring, nitrogen was passed through for 15 min, and ammonium persulfate, N,N-diethylacrylamide monomer, and N,N'-methylenebisacrylamide were added in sequence;

[0076] S22. Close the reaction flask, introduce nitrogen, heat the reaction flask to 40-60°C, react for 4-6h, and maintain nitrogen protection and stirring;

[0077] S23. After the reaction is completed, the reaction solution is cooled, the grafted polymer is separated by n-hexane precipitation and vacuum dried to constant weight to obtain a crude product;

[0078] S24. The crude product was extracted with acetone in a Soxhlet extractor for 24 h to remove the ungrafted homopolymer and monomer, and then vacuum dried to a constant weight to obtain a grafted copolymer.

[0079] The insecticide component is prepared into a microcapsule encapsulating the insecticide active ingredient with a sustained-release effect, and the preparation method comprises:

[0080] S31. Dissolve the modified sodium alginate in deionized water and stir to prepare a gel solution;

[0081] S32. Indoxacarb, matrine, huperzine A and piperidine are dissolved in ethanol, stirred and mixed, and then slowly added to the glue to form an oil-water emulsion, and the solution is dispersed into tiny droplets by a spray device or a dropping device;

[0082] S33. Under stirring conditions, the formed droplets were injected into a 1.5% CaCl2 solution. As the cross-linking reaction proceeded, the droplets gradually gelled to form microcapsules with a certain mechanical strength.

[0083] S34. The formed microcapsules are separated from the reaction system by filtration or centrifugation, and then washed with deionized water to remove possible residual crosslinking agent and other impurities;

[0084] S35. The washed microcapsules are vacuum dried to remove residual moisture.

[0085] A method for preparing a long-acting bait for killing red fire ants, which is applied to the long-acting bait for killing red fire ants, comprises the following steps:

[0086] S41. Preparation of modified sodium alginate;

[0087] S42. Preparation of insecticide component microcapsules;

[0088] S43. Preparation of modified glycerol;

[0089] S44. The corn, wheat and fructose are mixed uniformly according to the proportion, and then potassium sorbate, calcium propionate, modified glycerol and vitamin E are added to the mixed carrier material;

[0090] S45. The cured microcapsules were mixed with corn, wheat, fructose, potassium sorbate, calcium propionate and vitamin E in proportion, and modified glycerol was added to enhance wettability and adhesion;

[0091] S46. After the mixture is uniformly mixed in a blender, it can be formed by extrusion or die, and dried to remove moisture to obtain a long-lasting bait for killing red fire ants.

[0092] Example 2

[0093] This embodiment discloses a long-lasting bait for killing red fire ants, which is composed of the following raw materials by weight: 9 parts of insecticide, 25 parts of attractant, 10 parts of potassium sorbate, 10 parts of calcium propionate, 6 parts of vitamin E, and 7 parts of modified glycerol; the insecticide is the following raw materials by weight: 1 part of indoxacarb, 2 parts of matrine, 3 parts of huperzine A and 3 parts of piperidine; the attractant is the following raw materials by weight: 8 parts of corn, 8 parts of wheat and 8 parts of fructose. The insecticide components, by weight, also include: 4 parts of modified sodium alginate; the modified sodium alginate is generated by graft copolymerization of sodium alginate and poly (N, N-diethyl acrylamide), and has vinyl groups and diethylamino groups on the surface.

[0094] The preparation methods of the modified glycerol and modified sodium alginate in this embodiment are the same as those in Example 1. The preparation method of the long-acting bait for killing red fire ants in this embodiment is the same as that in Example 1.

[0095] Example 3

[0096] This embodiment discloses a long-acting bait for killing red fire ants, which is composed of the following raw materials in parts by weight: 4.1 parts of insecticide, 15 parts of attractant, 5 parts of potassium sorbate, 5 parts of calcium propionate, 2 parts of vitamin E, and 5 parts of modified glycerol; the insecticide is the following raw materials in parts by weight: 0.1 parts of indoxacarb, 1 part of matrine, and 2 parts of huperzine A

[0097] and 1 part of piperidine; the attractant is made of the following raw materials by weight: 5 parts of corn, 5 parts of wheat and 5 parts of fructose. The insecticide components, by weight, also include: 2 parts of modified sodium alginate; the modified sodium alginate is produced by graft copolymerization of sodium alginate and poly (N, N-diethyl acrylamide), and has vinyl groups and diethylamino groups on the surface.

[0098] The preparation methods of the modified glycerol and modified sodium alginate in this embodiment are the same as those in Example 1. The preparation method of the long-acting bait for killing red fire ants in this embodiment is the same as that in Example 1.

[0099] Example 4

[0100] This embodiment discloses a long-lasting bait for killing red fire ants, which is composed of the following raw materials by weight: 6.3 parts of insecticide, 20 parts of attractant, 7 parts of potassium sorbate, 7 parts of calcium propionate, 4 parts of vitamin E, and 6 parts of modified glycerol; the insecticide is the following raw materials by weight: 0.3 parts of indoxacarb, 1.5 parts of matrine, 2.5 parts of huperzine A and 2 parts of piperidine; the attractant is the following raw materials by weight: 6 parts of corn, 6 parts of wheat and 6 parts of fructose. The insecticide components, by weight, also include: 3 parts of modified sodium alginate; the modified sodium alginate is generated by graft copolymerization of sodium alginate and poly (N, N-diethyl acrylamide), and has vinyl groups and diethylamino groups on the surface.

[0101] The preparation methods of the modified glycerol and modified sodium alginate in this embodiment are the same as those in Example 1. The preparation method of the long-acting bait for killing red fire ants in this embodiment is the same as that in Example 1.

[0102] Example 5

[0103] This embodiment discloses a long-lasting bait for killing red fire ants, which is composed of the following raw materials by weight: 5.5 parts of insecticide, 18 parts of attractant, 7 parts of potassium sorbate, 8 parts of calcium propionate, 5 parts of vitamin E, and 6 parts of modified glycerol; the insecticide is the following raw materials by weight: 0.3 parts of indoxacarb, 2 parts of matrine, 2.2 parts of huperzine A and 1 part of piperidine; the attractant is the following raw materials by weight: 7 parts of corn, 6 parts of wheat and 7 parts of fructose. The insecticide components, by weight, also include: 2 parts of modified sodium alginate; the modified sodium alginate is generated by graft copolymerization of sodium alginate and poly (N, N-diethyl acrylamide), and has vinyl groups and diethylamino groups on the surface.

[0104] The preparation methods of the modified glycerol and modified sodium alginate in this embodiment are the same as those in Example 1. The preparation method of the long-acting bait for killing red fire ants in this embodiment is the same as that in Example 1.

[0105] Control group 1

[0106] The difference between this embodiment and embodiment 1 is that indoxacarb is not contained.

[0107] This embodiment discloses a long-lasting bait for killing red fire ants, which is composed of the following raw materials by weight: 6 parts of insecticide, 16 parts of attractant, 6 parts of potassium sorbate, 7 parts of calcium propionate, 3 parts of vitamin E, and 6 parts of modified glycerol; the insecticide is the following raw materials by weight: 1.5 parts of matrine, 2.5 parts of huperzine A and 1.8 parts of piperidine; the attractant is the following raw materials by weight: 6 parts of corn, 7 parts of wheat and 6 parts of fructose. The insecticide components, by weight, also include: 3 parts of modified sodium alginate; the modified sodium alginate is generated by graft copolymerization of sodium alginate and poly (N, N-diethyl acrylamide), and has vinyl groups and diethylamino groups on the surface.

[0108] The preparation methods of the modified glycerol and modified sodium alginate in this embodiment are the same as those in Example 1. The preparation method of the long-acting bait for killing red fire ants in this embodiment is the same as that in Example 1.

[0109] Control group 2

[0110] The difference between this embodiment and embodiment 1 is that matrine is not contained.

[0111] This embodiment discloses a long-lasting bait for killing red fire ants, which is composed of the following raw materials by weight: 6 parts of insecticide, 16 parts of attractant, 6 parts of potassium sorbate, 7 parts of calcium propionate, 3 parts of vitamin E, and 6 parts of modified glycerol; the insecticide is the following raw materials by weight: 0.2 parts of indoxacarb, 2.5 parts of huperzine A and 1.8 parts of piperidine; the attractant is the following raw materials by weight: 6 parts of corn, 7 parts of wheat and 6 parts of fructose. The insecticide components, by weight, also include: 3 parts of modified sodium alginate; the modified sodium alginate is generated by graft copolymerization of sodium alginate and poly (N, N-diethyl acrylamide), and has vinyl groups and diethylamino groups on the surface.

[0112] The preparation methods of the modified glycerol and modified sodium alginate in this embodiment are the same as those in Example 1. The preparation method of the long-acting bait for killing red fire ants in this embodiment is the same as that in Example 1.

[0113] Control group 3

[0114] The difference between this embodiment and embodiment 1 is that this embodiment does not contain huperzine A.

[0115] This embodiment discloses a long-lasting bait for killing red fire ants, which is composed of the following raw materials by weight: 6 parts of insecticide, 16 parts of attractant, 6 parts of potassium sorbate, 7 parts of calcium propionate, 3 parts of vitamin E, and 6 parts of modified glycerol; the insecticide is the following raw materials by weight: 0.2 parts of indoxacarb, 1.5 parts of matrine and 1.8 parts of piperidine; the attractant is the following raw materials by weight: 6 parts of corn, 7 parts of wheat and 6 parts of fructose. The insecticide components, by weight, also include: 3 parts of modified sodium alginate; the modified sodium alginate is generated by graft copolymerization of sodium alginate and poly (N, N-diethyl acrylamide), and has vinyl groups and diethylamino groups on the surface.

[0116] The preparation methods of the modified glycerol and modified sodium alginate in this embodiment are the same as those in Example 1. The preparation method of the long-acting bait for killing red fire ants in this embodiment is the same as that in Example 1.

[0117] Control group 4

[0118] The difference between this embodiment and embodiment 1 is that modified sodium alginate is not contained.

[0119] The present embodiment discloses a long-acting bait for killing red fire ants, which is composed of the following raw materials in parts by weight: 6 parts of insecticide, 16 parts of attractant, 6 parts of potassium sorbate, 7 parts of calcium propionate, 3 parts of vitamin E, and 6 parts of modified glycerol; the insecticide is the following raw materials in parts by weight: 0.2 parts of indoxacarb, 1.5 parts of matrine, 2.5 parts of huperzine A and 1.8 parts of piperidine; the attractant is the following raw materials in parts by weight: 6 parts of corn, 7 parts of wheat and 6 parts of fructose.

[0120] The preparation method of the modified glycerol in this embodiment is consistent with that in Example 1. The preparation method of the long-acting bait for killing red fire ants in this embodiment is consistent with that in Example 1.

[0121] Test verification: A nursery with obvious and evenly distributed red fire ant nests was selected and divided into 9 experimental plots of 20m×20m. No weeding or other pesticides were sprayed during the test to reduce human interference. Thirty ant nests were treated with pesticides in each plot, with the distance between each nest greater than 5m. The ant nests were marked with small red flags, and labels were hung to record the time and type of pesticides applied. The dosage of pesticides was 20g / nest.

[0122] When applying the pesticide, evenly spread a closed circle 10 cm away from the edge of the anthill, gradually approach the anthill and cover the top of the hill. When applying the pesticide, try to be as uniform as possible, spread lightly, and minimize the interference with the ant nest. There was no rain on the day of application, and a pre-drug base survey was conducted at 9:00 am. The pesticide was applied at 10:00 am. There was no rain within 6 hours after the application of the pesticide. The number of active red fire ant nests trapped was counted, and the control effect of the active red fire ant nests after the application was calculated to evaluate the control effect of the bait of the embodiment group and the control group.

[0123] Table 1 Effect of baits in the embodiment group and the control group on killing ant nests

[0124]

[0125] The effect of the baits of the embodiment group and the control group on killing ant nests is shown in Table 1. We can clearly see the significant difference in the killing effect of the embodiment group (different group distribution ratio) and the control group (lacking a certain component) on the red fire ant nest. The embodiment group performed well as a whole, with a control rate between 80.00% and 96.66%, among which embodiment 1 had the best effect, reaching a control rate of 96.66%. In contrast, the effect of the control group was significantly poorer, with a control rate of only between 36.66% and 66.66%. This difference highlights the importance of each component in the formula: control group 1 lacked indoxacarb, and its control rate dropped to 53.33%; control group 2 lacked matrine, and the control rate was 46.66%; control group 3 did not contain huperzine A, and the effect was the worst, only 36.66%; and control group 4 lacked modified sodium alginate, although it performed best in the control group, but the control rate of 66.66% was still far lower than any embodiment. This result strongly suggests that indoxacarb, matrine, huperzine A and modified sodium alginate all play key roles in the formulation, and the lack of any one of the ingredients will significantly reduce the ant killing effect of the bait. At the same time, the difference in effect between the groups in the examples also shows that the ratio of the components has an important influence on the final effect, and accurate mixing is required to achieve the best ant killing effect.

[0126] Finally, it should be noted that: Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A long-acting bait for killing red fire ants, characterized in that: The invention is composed of the following raw materials in parts by weight: 4 to 9 parts of insecticide, 15 to 25 parts of attractant, 5 to 10 parts of potassium sorbate, 5 to 10 parts of calcium propionate, 2 to 6 parts of vitamin E and 5 to 7 parts of modified glycerol; The insecticide is the following raw materials in parts by weight: 0.1-1 parts of indoxacarb, 1-2 parts of matrine, 2-3 parts of huperzine A and 1-3 parts of piperidine; The attractant is made of the following raw materials in parts by weight: 5 to 8 parts of corn, 5 to 8 parts of wheat and 5 to 8 parts of fructose.

2. A long-acting bait for killing red fire ants as claimed in claim 1, characterized in that: Calculated by weight, the insecticide components, calculated by weight, also include: 2 to 4 parts of modified sodium alginate; The modified sodium alginate is generated by graft copolymerization of sodium alginate and poly(N,N-diethylacrylamide), and has vinyl groups and diethylamino groups on the surface.

3. A long-acting bait for killing red fire ants as claimed in claim 1, characterized in that: The preparation method of the modified glycerol comprises: S11. Place a three-necked round-bottom flask in an ice bath, install a constant pressure dropping funnel, a thermometer and a nitrogen conduit, and ensure that the device is well sealed; S12. Slowly add glycerol and anhydrous dichloromethane into a three-necked flask, and turn on magnetic stirring to allow the glycerol to be fully dissolved; S13. Add sulfur trioxide - dimethylformamide complex and water dichloromethane to the constant pressure dropping funnel and mix them thoroughly; S14. Turn on nitrogen protection, place the reaction system in an inert atmosphere, control the reaction temperature between 0 and 5°C, slowly drop the sulfur trioxide-dimethylformamide complex solution into the glycerol solution, the dropwise addition time is about 1 to 1.5 hours, and after the dropwise addition is completed, continue to stir the reaction at 0 to 5°C for 2 to 3 hours; S15. The reaction mixture was slowly poured into ice water, stirred for 10 to 15 min, the organic layer and the aqueous layer were separated by a separatory funnel, and the organic layer was dried over anhydrous magnesium sulfate; S16. Filter and remove the desiccant, remove the solvent using a rotary evaporator, and the resulting crude product can be further purified by recrystallization or column chromatography.

4. A long-acting bait for killing red fire ants according to claim 2, characterized in that: The preparation method of the modified sodium alginate comprises: S21. Sodium alginate and deionized water were added to the reaction flask, dissolved by electromagnetic stirring, nitrogen was passed through for 15 min, and ammonium persulfate, N,N-diethylacrylamide monomer, and N,N'-methylenebisacrylamide were added in sequence; S22. Close the reaction flask, introduce nitrogen, heat the reaction flask to 40-60°C, react for 4-6h, and maintain nitrogen protection and stirring; S23. After the reaction is completed, the reaction solution is cooled, the grafted polymer is separated by n-hexane precipitation and vacuum dried to constant weight to obtain a crude product; S24. The crude product was extracted with acetone in a Soxhlet extractor for 24 h to remove the ungrafted homopolymer and monomer, and then vacuum dried to a constant weight to obtain a grafted copolymer.

5. The long-acting bait for killing red fire ants as claimed in claim 1, characterized in that: The insecticide component is prepared into a microcapsule encapsulating the insecticide active ingredient with a sustained-release effect, and the preparation method comprises: S31. Dissolve the modified sodium alginate in deionized water and stir to prepare a gel solution; S32. Indoxacarb, matrine, huperzine A and piperidine are dissolved in ethanol, stirred and mixed, and then slowly added to the glue to form an oil-water emulsion, and the solution is dispersed into tiny droplets by a spray device or a dropping device; S33. Under stirring conditions, the formed droplets were injected into a 1.5% CaCl2 solution. As the cross-linking reaction proceeded, the droplets gradually gelled to form microcapsules with a certain mechanical strength. S34. The formed microcapsules are separated from the reaction system by filtration or centrifugation, and then washed with deionized water to remove possible residual crosslinking agent and other impurities; S35. The washed microcapsules are vacuum dried to remove residual moisture.

6. A method for preparing a long-acting bait for killing red fire ants, which is used to prepare the long-acting bait for killing red fire ants as claimed in any one of claims 1 to 5, characterized in that: The method comprises the following steps: S41. Preparation of modified sodium alginate; S42. Preparation of insecticide component microcapsules; S43. Preparation of modified glycerol; S44. The corn, wheat and fructose are mixed uniformly according to the proportion, and then potassium sorbate, calcium propionate, modified glycerol and vitamin E are added to the mixed carrier material; S45. The cured microcapsules were mixed with corn, wheat, fructose, potassium sorbate, calcium propionate and vitamin E in proportion, and modified glycerol was added to enhance wettability and adhesion; S46. After the mixture is uniformly mixed in a blender, it can be formed by extrusion or die, and dried to remove moisture to obtain a long-lasting bait for killing red fire ants.

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