Application of undecanal in luring red imported fire ants

Abstract

The invention discloses an application of undecanal in luring red imported fire ants. According to the invention, it is found that volatile undecanal in a corpse pile of solenopsis invicta has an attraction effect on solenopsis invicta, and results show that 0.1-10 [mu] g / [mu] L of undecanal can obviously attract the solenopsis invicta, so that the corpse pile behavior of the solenopsis invicta occurs. Therefore, the solenopsis invicta attractant developed by undecanal is applied to areas with densely distributed solenopsis invicta, can trap the solenopsis invicta, and is used for efficient monitoring, prevention and control of the solenopsis invicta.

Description

Technical Field

[0001] The invention belongs to the technical field of integrated prevention and control of red fire ants, and particularly relates to the application of undecanal in luring red fire ants. Background Art

[0002] The red fire ant (Solenopsis invicta Buren) is native to South America and is one of the world's 100 most harmful alien pests. In recent years, with climate change and the development of global economic integration, it has spread to Fujian, Guangdong, Taiwan and other places in my country, causing huge losses to my country's agricultural ecological environment and social public security.

[0003] In the field, the prevention and control of red fire ants is still mainly chemical control, supplemented by physical and biological control. At the same time, monitoring and attracting is also one of the key factors for successful prevention and control. Among the different grades of red fire ants, only the worker ants are responsible for the task of going out of the nest to forage for food. At present, there are few attractants for red fire ant workers, and ham sausage is mainly used. Therefore, it is necessary to develop new red fire ant attractants for efficient monitoring and prevention of red fire ant workers. Summary of the invention

[0004] The purpose of the present invention is to provide a compound with a strong attractant effect on worker ants in response to the current problem of large-scale spread of red fire ants, which has caused significant losses to the ecological environment, public safety, infrastructure and human health, and specifically to the use of undecanal in attracting red fire ants.

[0005] The present invention confirms that undecanal has a strong attractant effect on red imported fire ants by using a headspace method combined with gas chromatography (GC-MC), an insect antennae potentiometer (EAG) and a behavior selection experiment.

[0006] The purpose of the present invention is to provide application of undecanal in attracting red imported fire ants.

[0007] Preferably, the red fire ants are worker ants of the red fire ant.

[0008] Preferably, the application is an application for triggering corpse-piling behavior of red fire ant workers.

[0009] Preferably, the use comprises the following steps: applying a red fire ant attractant containing undecanal to an area where red fire ants are to be attracted.

[0010] Preferably, undecanal is used as the active ingredient of the red fire ant attractant, and the concentration of undecanal in the red fire ant attractant is above 0.1 μg / μL; specifically, the concentration of undecanal can be 0.1 μg / μL, 1 μg / μL, 5 mg / mL, 10 μg / μL, etc.

[0011] Preferably, the concentration of undecanal in the red fire ant attractant is 0.1 μg / μL-10 μg / μL.

[0012] Beneficial effects of the present invention:

[0013] The behavioral selection assay experiment of the present invention has the advantages of simple preparation method, convenient operation, less experimental consumables, etc. The undecanal screened out by the present invention has a good attracting effect on red fire ant workers and can trigger the corpse stacking behavior of red fire ant workers, so it can be used for the development of red fire ant behavioral attractants, quarantine prevention and control, and efficient monitoring of red fire ants. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is the total ion current diagram of volatiles in the pile of red fire ant corpses determined by headspace method combined with GC-MS.

[0015] Figure 2 This is a graph showing the results of the antennal potential response of the antennae of red imported fire ants using the volatile substances undecanal and n-hexane; different letters indicate significant differences in the antennal potential response between different treatments (p<0.05).

[0016] Figure 3 This is a schematic diagram of the behavioral selection experimental setup.

[0017] Figure 4 It is a diagram of the behavioral selection of red fire ants triggered by different concentrations of undecanedal and n-hexane; A shows the situation of different concentrations of undecanedal (treatment group) and n-hexane (control group) inducing red fire ant workers to pile up corpses, and B is a comparison of the weight of corpses carried by red fire ant workers under the treatment of different concentrations of undecanedal and n-hexane; *** indicates a significant difference between the treatments of undecanedal and n-hexane (p<0.001). DETAILED DESCRIPTION

[0018] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.

[0019] Undecanal used in Examples 2 and 3 was purchased from Shanghai MacLean Biochemical Technology Co., Ltd. with a CAS number of 112-44-7.

[0020] Example 1: Identification and screening of volatiles from red fire ant carcasses by GC-MS

[0021] S1. Collection of Red Fire Ant Carcasses

[0022] First, we built an artificial ant nest, placed the test red fire ant nest in a plastic box (40cm×20cm×15cm) for breeding, and filled a 50mL centrifuge tube with water and stuffed it with cotton and placed it in the ant nest as a water test tube for the red fire ants to drink. After 24 hours of breeding, we collected 5g of worker ants and put them in a -80℃ refrigerator to kill them, and took them out of the refrigerator and placed them at room temperature for 24 hours for use.

[0023] S2. Identification of Compounds

[0024] The volatiles of the pile of red fire ants were collected by the headspace method. The pile collected by S1 was transferred to a sample bottle, and the headspace injector was inserted into the sample bottle and placed in a metal bath at 50°C for 30 minutes. After that, the headspace injector was pulled out and put on the machine for analysis and identification using a gas chromatograph-mass spectrometer. The specific conditions were: HP-5ms (30m, 0.25mm inner diameter, 0.25μm film thickness) capillary column, nitrogen carrier gas, injection port temperature 250°C, FID detector temperature 230°C, column box temperature program 60°C, 3°C / min to 236°C and maintained for 20 minutes, and then 3°C / min to 280°C and maintained for 8 minutes. GC used manual injection and non-divided mode, and the injection time was 1 minute.

[0025] like Figure 1 As shown, the analysis results show that geraniol has the highest content, followed by undecanal, phenylacetaldehyde, farnesene, etc.

[0026] Example 2: Insect antennal electroreaction (EAG) experiment to measure the potential response of undecanal to the antennae of red imported fire ants

[0027] A red imported fire ant worker was randomly selected, and the freshly excised head of the worker was mounted on a stand with two metal electrodes using conductive gel. The electrodes were the reference electrode and the recording electrode, respectively. Undecanal was dissolved in n-hexane into three concentration gradients, 0.1 μg / μL, 1 μg / μL, and 10 μg / μL, respectively. 20 μL of the test compound (i.e., different concentrations of undecanal, with n-hexane as the control) was pipetted onto filter paper (3×40 mm) and then gently shaken for 20 s under a fume hood to evaporate the n-hexane. The filter paper was inserted into a glass Pasteur pipette, and the tip of the pipette was mounted in the side hole of an L-shaped glass tube, with its direction facing the antennae. Each stimulation was 5 s, with an interval of 1 min to restore antennae activity. Each concentration gradient was repeated 5 times.

[0028] like Figure 2 As shown, the results showed that different concentrations of undecanal could induce EAG reaction in the antennae of red fire ants, and the degree of reaction increased with the increase of concentration.

[0029] Example 3: Experiment on the behavioral selection of red imported fire ant workers under different concentrations of undecanal

[0030] Put three plastic culture dishes (60mm in diameter) together in a triangle shape, and use a soldering iron to punch holes between two of the culture dishes. Due to the high temperature and the characteristics of the plastic, the three culture dishes will stick together in pairs, and the final shape will be as follows: Figure 3 As shown in the figure, the prepared device was divided into a treatment area (undecanal treated area) and a control area (hexane treated area), and filter papers (3mm×40mm) were placed in each area. 20μL of undecanal (undecanal was dissolved in hexane into three concentration gradients, 0.1μg / μL, 1μg / μL and 10μg / μL) were dropped on the filter paper in the treatment area, and 20μL of n-hexane was dropped on the filter paper in the control area. Both the treatment area and the control area waited for 20s to allow the n-hexane to evaporate, and 0.1g of worker ant corpses and 0.5g of worker ants were placed in the remaining area. A transparent glass plate was covered on the culture dish device to prevent the worker ants from escaping. After 3h, the weight of the corpses in the treatment area and the control area was weighed respectively, and the SPSS test was used to compare the significance of the weight of the corpses carried by the red fire ant workers.

[0031] like Figure 4 As shown, the results showed that in the behavioral selection experiment, compared with the control group, different concentrations of undecanal could significantly trigger the corpse piling behavior of red fire ant workers, and the amount of corpse piles in the culture dish of the treatment group was significantly higher than that of the control group, proving that undecanal is the key pheromone that triggers the corpse piling behavior of red fire ant workers and has a good attracting effect on red fire ant workers.

Claims

1. The application of undecanedal in attracting red fire ants.

2. The use according to claim 1, characterized in that: The red fire ants are worker ants.

3. The use according to claim 2, characterized in that: The application is an application for triggering the corpse piling behavior of red fire ants.

4. The use according to claim 1, characterized in that: The following steps are involved: A red fire ant attractant containing undecanal is applied to an area where red fire ants are to be attracted.

5. The use according to claim 4, characterized in that: Undecanal is used as an active ingredient of the red fire ant attractant, and the concentration of undecanal in the red fire ant attractant is above 0.1 μg / μL.

6. The use according to claim 5, characterized in that: The concentration of undecanal in the red fire ant attractant is 0.1 μg / μL-10 μg / μL.

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