The invention provides an oat culture medium and its preparation method and application, which comprises the following components by weight: 50-100 parts of oat kernels, 10-30 parts of sucrose, 5-20 parts of agar powder, 200-20 parts of 500 parts water. Preparation method: (1) Put oat kernels in a beaker, add water to it, and sterilize under high-pressure steam; (2) After sterilizing, let the sterilizer cool naturally, take it out and filter it with gauze, and add water to the filtrate , then add sucrose and agar powder, and continue heating to fully dissolve the sucrose and agar powder; The pot is naturally cooled to obtain the oat culture medium, and finally the prepared oat culture medium is refrigerated and preserved for future use. In the present invention, the oat culture medium is more suitable for the growth of Phytophthora infestans than the rye culture medium, and the cost of oat is low, the growth speed of Phytophthora infestans is fast, and other different fungi can be cultivated, and the growth of the fungi is good.