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Method for measuring quality of conidia of corynespora cassiicola

A multi-primary corynebacterium and conidia technology, applied in the field of microorganisms, can solve the problems of no equipment, tiny fungal spores, and inability to directly identify and count, achieving the effects of simple operation, improved speed and feasibility

Pending Publication Date: 2021-11-16
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention uses an analytical balance to weigh the quality of a large amount of spore powder, transforms unrealizable individual research into operable group research, and can effectively solve the problem that no instrument is available; the spores observed quantitatively by using a hemocytometer under an optical microscope The number can effectively solve the problem that the fungal spores are small and cannot be directly identified and counted by the naked eye; in addition, only 0.1mm of the blood cell counting plate is required 3 The number of spores in the counting area is counted, avoiding the time-consuming and laborious process of counting the number of all samples

Method used

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  • Method for measuring quality of conidia of corynespora cassiicola
  • Method for measuring quality of conidia of corynespora cassiicola
  • Method for measuring quality of conidia of corynespora cassiicola

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Screening Example 1 Spores preferred embodiment adhesive material

[0044]Clean adhesive material gently attaches a layer of spore powder on the surface of the multi-main base of the medium, so that the surface of the adhesive material is adhered to a layer of spore powder. The material of the adhesion spore powder was then carefully placed in an empty 1.5 ml of sterile centrifugal tube using tweezers, and the side side of the material was pressed against the side, 12000 rpm, centrifuged for 2 min.

[0045] The weighing paper, tin foil paper, common print paper total 3 materials, by observing the amount of spore powder collected by the centrifugal centrifugation and the clean level of the surface of the adhesive material after centrifugation, screening the optimum spore powder adhesive material .

[0046] Such as figure 1 As shown, the material that is easy to be centrifuged is a weighing paper and tin foil paper, and a large amount of spore remains after centrifugation of ...

Embodiment 2

[0047] Example 2, Best Formulation Method

[0048] The mass of spore powder was collected according to the bottom of the centrifuge tube, and the amount of solution was added to a spore suspension having a density of 1 mg / ml. Two solutions of sterile water and 70% ethanol were provided, and the solubility of the spore powder was observed by observing the sight of the vortex oscillation 5S and the wall condition in the centrifuge tube was determined. Subsequently, two treatments did not add abrasive beads and added abrasive beads in the centrifuge tube, and the optimal method for spore suspension configuration was determined by observing the degree of discrete degree of spores in the vortex oscillation 5S debris suspension.

[0049] Such as figure 2 As shown, it is possible to see that 70% ethanol dissolve spore powder is used, and the amount of spore suspension after the vortex oscillation is better than the use of sterile water, while the centrifugal wall residual liquid is sig...

Embodiment 3

[0050] Example 3, Determination of Multiple Principal Rock Spore mass

[0051] Using a pipette to absorb a polygon of 1 mg / ml of a polygonosporospore suspension, add to the blood cell count area, and statistical volume of 0.1mm under 100 / rl optical microscope. 3 The number of spores of the spores in the count zone, each repeatedly observed 10 vision. The quality of a single branchicospor is then calculated according to the formula (2).

[0052] Table 1 Multi-main Bacillus spore mass calculation data statistics

[0053]

[0054] As shown in Table 1, the mass of the multi-main base coryne monocratto was 4.95 to 6.25 ng, with an average of 5.69 ± 0.67 ng.

[0055] The method of the present invention is adapted to determine the mass of the spores of polygonal cephalosporospores, using analysis of the quality of the balance of large amounts of spore powder, and convert unalovable individual studies to operable group research, which can effectively solve the available equipment ava...

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Abstract

The invention discloses a method for determining the quality of conidia of corynespora cassiicola. The method comprises the following steps: activating and propagating a corynespora cassiicola strain to be tested, adhering conidia of corynespora cassiicola by using an adhesive material, and obtaining the mass of the corynespora cassiicola conidia by using a difference method; preparing a corynespora cassiicola conidia suspension liquid; and sucking the suspension, dropwise adding the suspension to the surface of a blood counting plate, counting the number of corynespora cassiicola conidia in a counting area under an optical microscope, and obtaining the mass of a single spore according to a formula 1. An analytical balance is used for weighing the mass of the spore powder, operation is easy, special instruments and equipment are not needed, and the method is suitable for most fungus species; the number of spores observed in the counting area is quantified through the blood cell counting plate under an optical microscope, the problem that fungal spores are small in size and cannot be directly counted by naked eyes can be effectively solved, only the number of spores in the counting area is counted, and the time-consuming and labor-consuming process for counting the number of all samples is avoided.

Description

Technical field [0001] The present invention relates to a multi-master rod conidial mass oxysporum measurement method, microorganisms belonging to the technical field. Background technique [0002] Multi-master stick cinerea fungus Corynespora cassiicola is a worldwide, widespread in 57 countries including China, the United States, the Netherlands and Japan. Bacteria very wide host range, can infect 90 families 252 genera 349 kinds of crops, including vegetables, fruits, flowers, grains, trees, etc., in which the rubber, cotton, soybeans and other crops, as well as cucumbers, tomatoes, beans and other vegetables the most serious hazards. Since 2005, my country's vegetable sticks Alternaria leaf spot occurs every year an area of ​​over 1,000 acres, more than 5 billion yuan of economic losses. Alternaria leaf spot, especially cucumber sticks, has been in Beijing, Shandong, Liaoning and other 19 provinces, municipalities, the field general incidence of 10% to 25%, severe up to 60% t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 柴阿丽李宝聚赵倩石延霞谢学文李磊范腾飞
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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