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A kind of oat culture medium and its preparation method and application

A culture medium and oat technology, applied in the field of culture medium, can solve the problems of high quality requirements, high rye market price, and difficulty in purchasing rye, and achieve high quality, low cost, and good fungal growth

Active Publication Date: 2022-02-18
雷波县春风农业综合开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Potato late blight is caused by the infection of Phytophthora infestans [Phytophthora infestans (Mont.) De Bary]. In the prior art, rye culture medium is the medium for cultivating Phytophthora infestans, but due to The quality of wheat is very high. It is not easy to buy purebred rye on the market. Most of them are triticale, and the market price of rye is relatively expensive.

Method used

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  • A kind of oat culture medium and its preparation method and application
  • A kind of oat culture medium and its preparation method and application
  • A kind of oat culture medium and its preparation method and application

Examples

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Effect test

Embodiment 1

[0040] A preparation method of oat culture medium, it comprises the following steps:

[0041] (1) Raw material preparation: 60g oat kernels, 20g sucrose, 8g agar powder, 1L water;

[0042] (2) Take 60g of oat kernels in a beaker, add 1L of water to it, mix, and sterilize at 121°C and 103.4kPa for 30 minutes;

[0043] (3) After being sterilized, let the sterilizer cool down naturally, take it out after cooling, filter it with 4 layers of gauze, add a small amount of water to the filtrate, add 20g sucrose and 8g agar powder, and continue heating. Stir once every minute to avoid sticking to the pan, and boil for 5 minutes to fully dissolve the sucrose and agar powder;

[0044] (4) Dilute the above-mentioned fully dissolved solution to 1L in a beaker, and then divide it into a triangular flask. After the division is completed, put a cotton plug on it, and wrap the cotton plug with kraft paper to prevent water from entering during sterilization. Wet the tampon, seal it, and steri...

Embodiment 2

[0047] A preparation method of oat culture medium, it comprises the following steps:

[0048] (1) Raw material preparation: 50g oat kernels, 30g sucrose, 5g agar powder, 0.6L water;

[0049] (2) Take 50g of oat kernels in a beaker, add 0.6L of water to it, mix, and sterilize at 121°C and 103.4kPa for 30 minutes;

[0050] (3) After sterilizing, let the sterilizer cool naturally, take it out after cooling, filter it with 4 layers of gauze, add a small amount of water to the filtrate, add 30g sucrose and 5g agar powder, continue heating, and keep stirring during the heating process Avoid sticking to the pan, boil for 8 minutes to fully dissolve the sucrose and agar powder;

[0051](4) Dilute the above-mentioned fully dissolved solution to 1L in a beaker, and then divide it into a triangular flask. After the division is completed, put a cotton plug on it, and wrap the cotton plug with kraft paper to prevent water from entering during sterilization. Wet the tampon, seal it, and s...

Embodiment 3

[0054] A preparation method of oat culture medium, it comprises the following steps:

[0055] (1) Raw material preparation: 100g oat kernels, 10g sucrose, 20g agar powder, 0.4L water;

[0056] (2) Take 100g of oat kernels in a beaker, add 0.4L of water to it, mix, and sterilize at 121°C and 103.4kPa for 30 minutes;

[0057] (3) After sterilizing, let the sterilizer cool down naturally, take it out after cooling and filter it with 4 layers of gauze, then add a small amount of water to the filtrate, then add 10g sucrose and 20g agar powder, continue heating, and keep stirring during the heating process Avoid sticking to the pan, boil for 7 minutes to fully dissolve the sucrose and agar powder;

[0058] (4) Dilute the above-mentioned fully dissolved solution to 1L in a beaker, and then divide it into a triangular flask. After the division is completed, put a cotton plug on it, and wrap the cotton plug with kraft paper to prevent water from entering during sterilization. Wet the...

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Abstract

The invention provides an oat culture medium and its preparation method and application, which comprises the following components by weight: 50-100 parts of oat kernels, 10-30 parts of sucrose, 5-20 parts of agar powder, 200-20 parts of 500 parts water. Preparation method: (1) Put oat kernels in a beaker, add water to it, and sterilize under high-pressure steam; (2) After sterilizing, let the sterilizer cool naturally, take it out and filter it with gauze, and add water to the filtrate , then add sucrose and agar powder, and continue heating to fully dissolve the sucrose and agar powder; The pot is naturally cooled to obtain the oat culture medium, and finally the prepared oat culture medium is refrigerated and preserved for future use. In the present invention, the oat culture medium is more suitable for the growth of Phytophthora infestans than the rye culture medium, and the cost of oat is low, the growth speed of Phytophthora infestans is fast, and other different fungi can be cultivated, and the growth of the fungi is good.

Description

technical field [0001] The invention belongs to the technical field of culture medium, and in particular relates to an oat culture medium and its preparation method and application. Background technique [0002] Potato late blight is caused by the infection of Phytophthora infestans [Phytophthora infestans (Mont.) De Bary]. The quality of wheat is very high, and it is not easy to buy purebred rye on the market. Most of them are triticale, and the market price of rye is relatively expensive. Therefore, it is necessary to find a cost-effective alternative rye medium to cultivate Phytophthora infestans. Contents of the invention [0003] In order to overcome the above-mentioned deficiencies, the inventor of the present invention through long-term exploration attempts and repeated experiments and efforts, continuous reform and innovation, proposed a kind of oat culture medium and its preparation method and application, its oat culture medium is better than black The wheat me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 李洪浩张鸿刘波微席亚东吴婕王燕平韩帅张河庆王晓黎
Owner 雷波县春风农业综合开发有限公司
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