129 results about "Phytophthora infestans" patented technology

A fungicidal composition for plants containing phosphonate (PO3) and phosphate (PO4) salts, and derivatives thereof is disclosed. The composition provides a single product which may be employed to control a Phytophthora infestans infection in plants.

The invention relates to the field of microbial control, in particular to bacillusamyloliquefaciens subsp Lh-1 and application thereof. With a preservation number of CGMCC No.8548, the bacillusamyloliquefaciens subsp Lh-1 is applied in control of Verticillium dahliae, erwinia carotovora, ralstonia solanacearum, valsa leucostoma, Valsa Mali Miyabe et Yamada, Phytophthora capsici and phytophthora infestans. According to the invention, the bacillusamyloliquefaciens subsp Lh-1 and pesticide and chitosan oligosaccharide with dual effects of fertilizer and pesticide are combined into an Lh-1 microecological agent, the viable count is small, the dosage is small, the production process is simple, the cost is low, and the Lh-1 microecological agent has the dual functions of disease prevention and growth promotion. Nursery test proves that reduce the disease incidence of crop is reduced, especially for control of Verticillium dahliae, the disease incidence drops to 6%, and the control effect is significant. In addition, the Lh-1 microecological agent is a biological strain preparation, is completely free of a series of problems caused by use of chemical pesticides, thus being conducive to pollution-free production of crops, and farmers can have no need for pesticide or reduce the dosage of chemical pesticide, thus greatly saving the expenditure.

The invention relates to a method for obtaining a capsicum phytophthora resistance candidate gene and a molecular marker, and application. The method is used for obtaining the capsicum phytophthora resistance candidate gene by utilizing capsicum phytophthora transcriptome and whole-genome sequencing data information, differentially-expressed gene identification, bioinformatics analysis, molecular marker development and phytophthora inoculation identification and belongs to the technical field of capsicum biology. The method comprises the following steps: sequencing a phytophthora resistant and susceptible gene pool transcriptome obtained after phytophthora inoculation of an F2 population constructed by capsicum highly-resistant and highly-susceptible phytophthora materials, performing expression analysis and functional annotation on differential genes, extracting DNAs (Desoxvribose Nucleic Acid) of a capsicum phytophthora highly-resistant and highly-susceptible phytophthora material genome, performing primer design and PCR (Polymerase Chain Reaction) amplification, performing sequence difference analysis and SNP site identification, performing SNP specific primer design and validity verification, and performing other steps to efficiently obtain the capsicum phytophthora resistance candidate gene and the molecular marker. According to the method, the capsicum phytophthora resistance candidate gene can be accurately identified, and the effective molecular marker can be developed.

A Solanum lycopersicum plant including within its genome at least one Tomato Spotted Wilt Virus (TSWV) resistance allele and at least one Phytophthora infestans resistance allele. The resistance alleles are present in the coupling phase at different loci on one chromosome and the plant is resistant against TSWV and resistant against at least Phytophthora infestans. A method for producing a hybrid Solanum lycopersicum plant including (a) obtaining a Solanum lycopersicum plant having within its genome at least one Tomato Spotted Wilt Virus (TSWV) resistance allele and at least one Phytophthora infestans resistance allele in the coupling phase; and (b) crossing the Solanum lycopersicum plant with a second Solanum lycopersicum plant bearing an additional resistance allele.

The utility model relates to a sterilization composite containing azoxystrobin and application. The composite mainly comprises two compounds A and B with effective quantity, the A is the azoxystrobin, the B is selected one from myclobutanil, flusilazole, mancozeb and dimethomorph, and the compounds A and B have the following parts by weight: 50-1 and 1-50. The composite can be used for preventingand curing powdery mildew, rust disease, downy mildew, anthracnose, phytophthora infestans, leaf spot, cladosporium cucumerinum and the like on frumentum, vegetables and fruit trees, and particularlyhas good effect on sigatoka, erysiphe graminis, phytophthora capsici leonian, cucumber downy mildew and venturia nashicola.

A Solanum bulbocastanum gene has been cloned from a late blight-resistance locus and its sequence provided, together with the sequence of the encoded protein. DNA encoding the resistance protein has been introduced into potato plants and confers resistance to Phytophthora infestans, the causal agent of late blight. The resistance protein is in the class of Nucleotide Binding Site-Leucine-Rich Repeat Proteins (NBS-LRRP), and the gene in S. bulbocastanum is flanked by related NBS-LRRP gene sequences.

The invention relates to a heterocycle substituted 1,3,4-oxadiazole compound and a preparation method and application thereof. The compound includes the following steps shown in the general formula (I) in the description. The compound has a good inhibitory effect on pathogenic bacteria and fungi, such as rice white leaf diseases, citrus canker diseases, pepper wilt pathogenic bacteria, blueberry phytophthora lateralis, wheat gibberella saubinetii, potato phytophthora infestans, oilseed rape sclerotinia sclerotiorum, dragon-fruit colletotrichum orbiculare and the like.

Disclosed are isolated nucleic acid molecules from Solanum tuberosum which are capable of conferring resistance against plant pathogens including Phytophthora infestans. Transgenic plants and plant cells comprising the nucleic acid molecules are also disclosed.

The invention discloses streptomyces hygroscopicus capable of producing salicylic acid and rapamycin and application of the streptomyces hygroscopicus to prevention and control of plant oomycetes andfungal diseases. The invention also discloses the streptomyces hygroscopicus capable of producing the salicylic acid and the rapamycin, which has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 15518, and the application of the streptomyces hygroscopicus to the prevention and control of the plant oomycetes and fungal diseases. The invention identifiesa biocontrol bacterium aiming at the plant oomycetes and fungal diseases, especially phytophthora infestans and fusarium oxysporum; the bacterium can be used for remarkably inhibiting spore germination and mycelium growth of the phytophthora infestans; an effective method is provided for biologically preventing and controlling the plant oomycetes and fungal diseases, especially the phytophthora infestans and fusarium wilt. The method disclosed by the invention can be used for effectively preventing and controlling the phytophthora infestans, the fusarium wilt and other plant diseases and reducing the economic loss; the method has important meaning of reducing the utilization of pesticide and alleviating environment pollution, and also has significant application value of keeping the balance and sustainable development of an ecological system.

The invention relates to a process which induces potato late blight pathogen to generate pathogenic secretory protein, which comprises placing potato late blight pathogen sample in common water agar culture medium to hydrate and cultivate, selecting a little amount of bacterial filament to graft in the common solid culture medium through a grafting needle when hoary moldy layers grow on the leaves, cultivating in dark under 16-18 DEG C, transferring bacterial filament blocks into 100mL pathogenic protein liquid inducing medium after forming a larger bacterial colony, removing bacterial filament bodies by double-layer sterilizing filtering paper vibrating in 150 rpm for 14-16days under 18 DEG C, centrifuging the filtering liquid in 4000rpm for 10 min , adding ammonium sulfate (697g / L) in supernatant fluid, centrifuging in 10000rpm for 20min conducting dialysis treatment to the sedimentation, obtaining potato late blight pathogen pathogenic secretory protein abstract after freeze drying, and reserving under -20 DEG C. The process can induce potato late blight pathogen to generate a great amount of pathogenic secretory protein, fills the blank that pathogenic secretory protein can not be obtained in potato late blight pathogen pathogenic mechanism research at home and board, and has great significance.

The invention discloses a biocontrol inoculant for controlling potato late blight, and belongs to the technical field of crop disease control. The biocontrol inoculant is fermented by streptomyceseuropaeiscabiei St19. A strain is registered and preserved in China General Microbiological Culture Collection Center (CGMCC) on March 24, 2014; and the preservation number is CGMCCNO.8962. The biocontrol inoculant can inhibit growth of potato phytophthora infestans, represents a better control effect on the potato late blight, can be used for root irrigation in a seedling stage or an adult-plant stage of a potato, or can be mixed with an organic fertilizer to prepare a microbial fertilizer. The St19 strain is obtained from potato rhizosphere soil, is harmonious and compatible with soil ecology, and is non-toxic and non-virulent, so that the strain has a larger application potential in biological control of the potato late blight.

The invention relates to a method of treatment and control of potato late blight of seed potato and pertains to the pesticide application field. The steps of the invention are: (1) seed potato treatment: weighing the seed potato of 50 to 300 kg / Mu to be sliced and piled in a drying place and evened off with thickness of 10 to 15 cm one to three days before potato planting; (2) taking 58 percent of Fubol wettable powder of 1 to 6g / Mu to be dissolved in clean water of 0.5 to 2.5kg ; (3) an atomizer is used for evenly spraying liquor on potato slices and stops spraying when the slices are all wet; the slices are dried naturally without being stirred; (4) the potato slices can be used for planting after 2 to 10 days. The invention overcomes the defects of the prior art of the seed potato treatment, which is particularly aims at the characters that tuber of the seed potato is large, slicing is done before planting, other germina are carried with, phytophthora infestans is serious and the seed potato treatment is difficult, proposes a treatment method with simple and easy application, low cost and high effectiveness and without diffusion of bacterial diseases when in control of the potato late blight.

The invention provides a primer, a test kit containing the primer and a detection method for detecting phytophthora infestans, the utilization of the primer and the detection method for detecting the phytophthora infestans can realize high accuracy, strong specificity and high sensitivity, the method of the invention has the advantages of accuracy, speediness, simplicity, easy operation and the like and can identify pathogens at the early stage of infection of the disease and simultaneously detect the pathogens in field soil, and the invention has great significance for preventing and treating late bright of potatoes and tomatoes. Simultaneously, the invention also has the great significance for reducing the blind use of pesticides, reducing the production cost and reducing the environmental pollution of the pesticides.

The invention relates to an application of an isoflavonoids compound, that is, glabridin, to prepare an agricultural bactericide. The compound is used for inhibiting the following pathogenic funguses of crops: fusarium graminearum, colletotrichum gloeosporioides, botrytis cinerea, phytophthora infestans, or pyricularia grisea, and the following pathogenic bacteria of crops: erwina carotovora, pseudomonas solanacearum, ralstonia solanacearum, or pseudomonas syringae pv. actinidae. Furthermore, the compound can be prepared into various formulations with other agricultural auxiliaries, and the compound has a synergistic effect after combined with shikonin according to a certain weight ratio.

The invention provides a set of production technology for preventing ralstonia solanacearum from growing and reproducing in solanaceae crops to increase crop yield by aiming at the defects of the prior art for controlling the damages of the ralstonia solanacearum to the crops. Brevibacillus choshinensis X23 has a wide bacteriostatic spectrum and good environmental safety and has antagonism effects to various pathogenic fungi, such as phytophthora parasitica var nicotianae, pyricularia grisea, alternaria alternate, phytophthora infestans and the like, so that the brevibacillus choshinensis X23 has favorable development prospect and application potential.

The invention belongs to a method for preparing inula japonica plant extract and applications of the inula japonica extract. The plant material (root, stem, leaves, inflorescence and whole grass) of the inula japonica (including Inula japonica and Inula britannica) is crushed and extracted by a solvent which comprises 95% of alcohol; and the extract solution is concentrated to have the same weight with the inula japonica crushed matters, thus obtaining inula japonica extract. The inula japonica extract has obvious inhibition effects to cucumber botrytis cinerea, alternaria solani, cladosporium fulvum, colletotrichum lagenarium, sphaerotheca fuliginea, pseudoperonospora cubensis, potato phytophthora infestans, tomato phytophthora infestans and rhizoctonia cerialis; wherein the inflorescence extract has strong antibacterial activity. The inula japonica extract extracted by alcohol can be matched with suitable solvent and pesticide adjuvant to prepare britannica lactone emulsifiable solution and britannica lactione micro-emulsion of botanical fungicide. The inula japonica extract has obvious effects for preventing cucumber downy mildew, sphaerotheca fuliginea, tomato gray mold and tomato leaf mould, is especially suitable for prevention and control on the fungi diseases to economical crops such as vegetables and the like, and is a botanical fungicide with nuisanceless.

The invention relates to a bactericidal composition containing fluopicolide and mandipropamid. Effective components of the bactericidal composition are the fluopicolide and the mandipropamid which arecompounded, wherein the mass ratio of the fluopicolide to the mandipropamid is (1 to 80) to (80 to 1); the total mass of the fluopicolide and the mandipropamid as the effective components in a preparation accounts for 1 to 80 percent of the total mass of the whole preparation, and other components are pesticide allowable and acceptable auxiliary components. The bactericidal composition disclosedby the invention can be prepared into pesticide dosage forms of emulsifiable concentrate, suspending agents, wettable powder, water dispersible granules, emulsion in water and microemulsion, is mainlyused for preventing and controlling gray mold, fusarium wilt and downy mildew of crops such as cucumber, tomatoes, grapes, strawberries, peas and fragrant-flowered garlic as well as scab and alternaria leaf spot of fruit trees, and has excellent prevention and treatment effects on phytophthora infestans, cucumber anthracnose and powdery mildew of leaves.

The invention relates to a 1, 3, 4-oxadiazole hydrazide compound as well as a preparation method and application thereof. The compound has a structure as shown in a general formula (I), according to the invention, the method includes taking a 1, 3, 4-oxadiazole compound as a basis and introducing hydrazide into the system; the compound has a good inhibition effect on plant pathogenic bacteria, fungi and oomycetes, and has a good inhibition effect on pathogenic bacteria such as rice bacterial leaf blight, wheat scab, pepper fusarium wilt, sclerotinia sclerotiorum, colletotrichum gloeosporioides, phytophthora infestans, blueberry root rot and the like.

The invention discloses a pyrazolyl-oxa(thia)diazole compound containing pyridinium salt, and a preparation method and application thereof. The compound has the structure as shown in the following general formula (I). On the basis of a pyrazolyl-oxa(thia)diazole thioether(oxoether) compound, a pyridinium group capable of improving the biological activity of a target compound is introduced into the system to synthesize a series of pyrazolyl-oxa(thia)diazole oxoether or thioether amphipathic molecules containing the pyridinium salt; moreover, the compound has a good inhibition effect on pathogenic bacteria and fungi, has good inhibition effects on bacteria (bacterial leaf blight of rice, ralstonia solanacearum, xanthomonas citri and the like) and fungi (gibberella zeae, phytophthora infestans, rhizoctonia solani, botrytis cinerea pers, fusarium oxysporum, apple rot pathogenic bacteria and the like), and provides an important scientific base for research, development and creation of novel pesticides.

Bacterial strains, or extracts thereof, are effective to control, treat or prevent infection of solanaceous plants with Phytophthora infestans, the causative agent of potato late blight disease. Alsoprovided are biopesticidal formulations containing one or more of the bacterial strains or extracts thereof, and the use of the bacterial strains, extracts or biopesticidal formulations in the control, treatment and / or prevention of potato late blight disease.

This invention provides novel gene sequences, compositions and methods for enhancing the resistance in crops, in particular but not limited to, potato, to late blight caused by the oomycete pathogen Phytophthora infestans.

The present invention relates to a plant belonging to the Solanaceae family wherein said plant comprises a genetic trait providing Phytophthora resistance and wherein said resistance trait is encoded by a combination of at least two genes having a reduced expression, or transcription, of said genes or a reduced activity of proteins encoded by said genes as compared to said plant belonging to Solanaceae family being susceptible to Phytophthora.

The invention belongs to the field of genetic engineering and discloses a loop-mediated isothermal amplification primer composition for detecting phytophthora infestans and application thereof. A detection target of the phytophthora infestans is PiSMC; the nucleotide sequence of the phytophthora infestans is shown as SEQ ID NO. 1; a forward internal primer FIP of a specific LAMP primer compositionof the target sequence is shown as SEQ ID NO. 2; a reverse internal primer BIP is shown as SEQ ID NO. 3; a forward external primer F3 is shown as SEQ ID NO. 4; a reverse external primer B3 is shown as SEQ ID NO. 5; a reverse loop primer LB is shown as SEQ ID NO. 6. The detection system is capable of rapidly and efficiently detecting the phytophthora infestans with high specificity and high sensitivity under the LAMP amplification condition; meanwhile, the detection results can be observed by naked eyes; therefore, the detection system has a great value of application in detection of late blight of potatoes in the field.

The invention discloses a bactericide composition of thifluzamide and fluazinam. The bactericide composition comprises common accessory ingredient, thifluzamide and fluazinam, wherein the mass fraction ratio of the thifluzamide to the fluazinam is (10 to 1) to (1 to 15), and the preferred ratio is (6 to 1) to (1to 9). After the thifluzamide and the fluazinam are compounded, the effect is improved remarkably, and the dosage of the effective components of compounded medicament is less than that of the effective components used separately, so that the bactericide composition is suitable for preventing and curing diseases caused by plant pathogens, such as phytophthora infestans, plasmodiophoromycetes and botrytis cinerea.

The invention discloses a method for detecting three potato pathogenic bacteria by multiple PCR (polymerase chain reaction) techniques, and belongs to the technical field of biological detection. According to the method, special genes of three pathogenic bacteria including phytophthora infestans, alternaria solani and rhizoctonia solani AG3 anastomosis groups are simultaneously amplified by multiple PCR once, and a PCR amplifying product is detected through agarose gel electrophoresis. The method includes the steps: (1) extraction of genome DNA (deoxyribonucleic acid) of the three potato pathogenic bacteria; (2) design of three pairs of amplification primers; (3) implementation of multiple PCR; (4) electrophoresis detection. According to the method, the three pathogenic bacteria are simultaneously detected by the multiple PCR techniques, and the method has the advantages that efficiency is high, speed is high, time is saved, and cost is reduced.

A biological material is disclosed for exerting antagonism against vegetable pathogens, which contains Bacillus mojavensis R3B mutant strain deposited under # NCAIM (P) B 001389 according to the Budapest Treaty. The biological material according to the invention is an effective antagonist against the pathogens of vegetables, preferably tomato, pepper, lettuce and / or cabbage, in particular against the pathogens selected from the group of Xanthomonas vesicatoria, Pseudomonas syringae and Clavibacter michiganensis vegetable pathogen bacteria and Pythium debaryanum, Phytophthora infestans, Alternaria alternata and Fusarium oxysporum vegetable pathogen fungi. Furthermore, the invention relates to a composition comprising the biological material according to the invention and optionally a copper-containing pesticide and a process for controlling vegetable pathogens, furthermore use of the biological material or composition according to the invention for the protection of vegetables, preferably tomato, pepper, lettuce and / or cabbage.

The invention discloses a bactericidal composition containing a plant extract. The composition contains a plant tissue extract A and benziothiazolinone, wherein the plant tissue extract A is extracted from a mixture of herba aconitum rotundifolium, iris tectorum rhizome, hibiscus leaves and herba madder; the plant tissue extract A and the benziothiazolinone have a synergistic effect on phytophthora infestans when being mixed for use; the herba aconitum rotundifolium, the iris tectorum rhizome, the hibiscus leaves and the herba madder are all fresh plant tissues; the fresh plant tissues mean plant tissues of which a rate of water loss is smaller than 5% after the plant tissues leave from a growing environment of the plant tissues. The bactericidal composition disclosed by the invention is applicable to prevent and treat crop diseases such as potato late blight, pepper phytophthora blight, cucumber downy mildew, litchi downy blight and the like. The bactericidal composition effectively, scientifically and reasonably utilizes resources, has an important significance for protecting the environment, and takes an active promoting effect on agricultural input and increase of both production and income in the agricultural industry.

The invention discloses an LAMP (Loop-mediated Isothermal Amplification) primer combination for detecting tomato phytophthora infestans and application. The LAMP primer combination for detecting the tomato phytophthora infestans consists of four specific primers, and the primer combination disclosed by the invention is applied in the LAMP detection of the tomato phytophthora infestans. A detection system disclosed by the invention is incubated under the isothermal condition of 64 DEG C for 1 hour, SYBR green I developer is adopted for detection by development, and with whether green fluorescence is observed as a standard, a detection result is judged. The LAMP primer combination has the advantages of high accuracy, high specificity, convenience in operation and high practicability, does not need complex instruments, realizes isothermal amplification, provides a novel molecular detection technique for the tomato phytophthora infestans, can be used in rapid, sensitive and accurate detection in the early stage or infection incubation of the tomato phytophthora infestans in production practice, and provides a reliable technical and theoretical basis for the prevention and treatment of the tomato phytophthora infestans.

The invention relates to a pesticide composition containing valifenalate and application thereof. Effective components of the pesticide composition comprise a component A and a component B, wherein the component A is the valifenalate and the component B is selected from one of benthiavalicarb-isopropyl, fluopicolide, fluazinam and mandipropamid; the weight part ratio of the component A to the component B ranges from (30 to 1) to (1 to 30). The composition provided by the invention can be used for preparing agriculturally acceptable missible oil, a suspension agent, water dispersible granules, wettable powder and emulsion in water. According to the pesticide composition, the effective components are reasonably matched, the activity and the sterilization effect of the composition are not simple superposition of the activity of all the components and the compound synergistic effect is remarkable; the usage amount of the effective components of a compound medicament chemical agent is remarkably reduced when being compared with that of single utilization of the effective components. The pesticide composition containing the valifenalate, provided by the invention, is suitable for preventing and controlling diseases caused by oomycete pathogenic bacteria, especially phytophthora capsici leonian, phytophthora infestans and downy mildew of various crops.

The invention discloses a rapid immunoassay test strip for phytophthora infestans and application thereof. The test strip comprises a bottom plate, wherein a water absorption pad, a detection pad, a gold label pad and a sample pad are sequentially adhered to one surface of the bottom plate from top to bottom; every two adjacent pads are connected in an overlapped mode at the joint, a nitrocellulose membrane serves as a base pad of the detection pad, a quality control line and a detection line are transversely arranged on the nitrocellulose membrane from top to bottom, the detection line is located below the quality control line, the detection line is coated with an anti-pyricularia grisea polyclonal antibody 0550, and the quality control line is coated with goat anti-mouse IgG. The gold label pad is sprayed with a nanogold labeled anti-pyricularia oryzae monoclonal antibody. The specificity is high, and no cross reaction is caused to pythium aphanidermatum, pear rust water, pear rot, pear anthracnose and the like of Asian pears; the sensitivity of the test strip can reach 1*10<5> cfu / mL, and the test strip can be used for rapid screening of pythium aphanidermatum in fields.