22 results about "Phytophthora infestans" patented technology

The invention relates to application of mitogen-activated protein kinase PiPmk1 in screening molecules for inhibiting formation of a phytophthora infestans infection structure. The invention finds that the molecular marker plays a key role in the development of a phytophthora infestans cyst bud tube and / or the formation of an infection structure, which indicates that the molecular marker has the potential of serving as a target for inhibiting phytophthora infestans infection, and a corresponding molecular screening method and a generative prediction model are further developed and utilized; rapid and accurate prediction and screening of phytophthora infestans infection inhibitory molecules are realized, the research and development period and cost are greatly shortened, and a new thought and a new method are provided for development of accurate targeted green pesticides.

The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.

The invention discloses an endophytic subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. The strain can inhibit the growth of different physiological races of phytophthora infestans, and has inhibitory activity on various pathogenic oomycetes. The fermentation liquor of the strain contains micromolecular antibacterial substances with relatively high stability, and has remarkable antibacterial activity; protein components in fermentation liquor can induce plants to generate resistance, and infection of phytophthora infestans is effectively inhibited. Different types of microbial agents prepared by utilizing the strain DS1 can be used for effectively preventing and treating potato late blight, and the microbial preparation is used for seed dressing treatment of potatoes and wheat and can be used for remarkably promoting the growth of crops.

The present invention relates to bio-fungicides that inhibits Phytophthora infestans. More particularly, the present invention relates to novel strains of Bacillus spp. that can inhibit Phytophthora infestans. The present invention also relates to bio-fungicidal formulations comprising novel strains of Bacillus spp. either alone or a mixture of novel strains of Bacillus spp., in combination with other excipients.

The invention provides application of a DTX29 gene and a protein thereof in improving the resistance of plants to phytophthora infestans. The invention finds a susceptible gene DTX29 capable of effectively improving the resistance of plants to phytophthora infestans through a large number of researches. The DTX29 gene of the plant is edited through a CRISPR-Cas technology and the like, so that the function of the DTX29 gene is lost, the resistance of the plant to phytophthora infestans can be effectively improved, the diameter and area of disease spots and the relative biomass of phytophthora infestans are reduced, and normal growth and development of the plant are not influenced. The invention provides a new target and idea for prevention and control of plant diseases caused by phytophthora infestans.

The invention provides application of an SlSRC2 gene in regulation and control of tomato broad-spectrum disease resistance, AtSRC2 in arabidopsis thaliana is retrieved through NCBI (National Center of Biotechnology Information), BLAST analysis is performed in a tomato genome by using an amino acid sequence of the AtSRC2, and a protein, namely the SlSRC2, which has the highest similarity with the AtSRC2 in tomatoes is found. Through overexpression of the CDS region of the SlSRC2 in the tomato, the purpose of overexpression of the SlSRC2 is achieved, the alkaloid content in the tomato can be reduced, and the resistance to botrytis cinerea, phytophthora infestans and tomato yellow leaf curl virus is weakened; on the other hand, gene editing is conducted on the specific sequence of the SlSRC2 gene in the tomato by means of the CRISPR-Cas9 technology, the alkaloid content of the obtained mutant plant can be increased, and the resistance of the mutant plant to botrytis cinerea, phytophthora infestans and tomato yellow leaf curl virus is enhanced.

The invention provides a potato late blight biocontrol strain as well as a biocontrol inoculant and application thereof, and belongs to the technical field of biocontrol bacteria. The invention discloses a bacillus safeensis strain B1, and the biological preservation number of the bacillus safeensis strain B1 is CGMCC (China General Microbiological Culture Collection Center) No.36024. The invention also provides a biocontrol bacterium composition which is prepared by compounding the Leifsonia sp. Strain L1 alone or by combining the Leifsonia sp. Strain L1 with the Leifsonia sp. Strain L6 and the strain B1. The strain B1 has good activity of inhibiting phytophthora infestans, meanwhile, the biocontrol bacterium composition not only further improves the antibacterial activity of the strain B1, but also shows that the biocontrol bacterium composition can effectively reduce the disease index of potatoes, and compared with the single-phase strain B1, the biocontrol bacterium composition can effectively improve the prevention effect of potato late blight.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of PP2C family genes in improving resistance of tomatoes to phytophthora infestans. Two genes related to phytophthora infestans infection are found and verified in tomatoes, the genome nucleotide sequences of the two genes are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes belong to PP2C gene family members. The gene is knocked out or silenced through a gene editing technology, and it is found that the tomato strain with the gene knocked out or silenced has higher phytophthora infestans resistance compared with a wild type. Meanwhile, tomato strains edited by the genes are not obviously influenced in the aspects of growth and development. Therefore, the invention not only enriches the research of phytophthora infestans susceptibility related genes in tomatoes, but also develops a tomato strain with enhanced phytophthora infestans resistance by utilizing the characteristics of the genes, and provides a new technical strategy and gene resources for tomato breeding.

The invention provides a biocontrol penicillium and application thereof, the biocontrol penicillium is Penicillium oxalicum POIL-1, the biocontrol penicillium is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.42328. The biocontrol penicillium has the advantages that the biocontrol penicillium is the Penicillium oxalicum POIL-1, and the biocontrol penicillium is the Penicillium oxalicum POIL-1; it is found for the first time that the strain or culture filtrate of the strain can effectively inhibit various common potato pathogenic bacteria including phytophthora infestans, can be used for field biological control of major diseases such as potato late blight and the like, and is beneficial to reduction of use of chemical pesticides. In addition, the strain significantly promotes the growth of solanaceae crops such as potatoes, tobaccos and tomatoes, has good phosphate solubilizing ability, and is helpful for improving the crop yield and reducing the use of phosphate fertilizer. The Penicillium oxalicum POIL-1 strain can be used for biological pesticide development, has triple effects of preventing diseases, promoting growth and improving soil quality, is green and environment-friendly, is low in cost, and has good application potential.

This invention relates to the field of genetic engineering technology, and particularly to soybean GmST3 protein mutants and their application in regulating soybean resistance to Phytophthora infestans. This invention reveals for the first time that the soybean GmST3 protein and its encoding gene have the function of regulating soybean resistance to Phytophthora infestans. By reducing the expression of wild-type soybean GmST3 protein or its encoding gene, or by inducing soybean plants to express soybean GmST3 protein mutants, soybean resistance to Phytophthora infestans can be effectively improved. The soybean GmST3 protein mutants and their encoding genes provided by this invention can also be used for the breeding of soybean Phytophthora-resistant varieties, and have broad application prospects.

The application discloses a kind of polyphenol oxidase StPPO9 The application discloses application of a gene in improving plant resistance to late blight, and belongs to the field of plant genetic engineering. StPPO9 The nucleotide sequence of the gene is shown as SEQ ID No.1, and the amino acid sequence encoded by the gene is shown as SEQ ID No.2. StPPO9 By overexpressing the gene in plants (especially potatoes), the application can significantly activate the defense response of the plants, thereby enhancing the resistance of the plants to late blight caused by Phytophthora infestans. The application also provides a specific primer pair for cloning the gene, a protein encoded by the gene, and application of a recombinant overexpression vector containing the gene and an engineering bacterium in disease-resistant breeding. The application provides an effective gene resource and a biotechnological means for cultivating new potato and other solanaceous crop varieties resistant to late blight.

The invention discloses an application of selenomethionine in any one of the following applications: (1) an application in inhibition of Phytophthora infestans (Phytophthora infestans) or pathogenic fungi, and (2) an application in inhibition of the Phytophthora infestans (Phytophthora infestans); (2) application in preparation of products for inhibiting phytophthora infestans or pathogenic fungi; (3) application in prevention and treatment of plant late blight or diseases caused by pathogenic fungi; (4) application in preparation of products for preventing and treating plant late blight or diseases caused by pathogenic fungi; (5) application in plant growth promotion; and (6) application in preparation of the plant growth promoter. The invention provides a novel green pesticide product and novel application of selenomethionine in prevention and treatment of pathogenic bacteria and related plant diseases. A novel green pesticide is provided for plant late blight, and the use of chemical pesticides can be reduced.

The application belongs to the technical field of molecular biology breeding, and particularly relates to application of EF Hand family genes in improving resistance of tomatoes to pathogenic Phytophthora. Two pathogenic Phytophthora disease-related genes are found and verified in tomatoes, the genomic nucleotide sequences of which are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes both belong to members of the EF Hand gene family. By knocking out or silencing the above genes through gene editing technology, it is found that the tomato lines with the above genes knocked out or silenced have higher resistance to pathogenic Phytophthora compared with wild types. Meanwhile, the gene-edited tomato lines are not obviously affected in growth and development. Therefore, the application not only enriches the research on pathogenic Phytophthora disease-related genes in tomatoes, but also develops tomato lines with enhanced resistance to pathogenic Phytophthora by using the characteristics of the above genes, thereby providing new technical strategies and gene resources for tomato breeding.

The invention discloses application of a polyphenol oxidase StPPO9 gene to improvement of late blight resistance of plants, and belongs to the field of plant genetic engineering. The nucleotide sequence of the StPPO9 gene is as shown in SEQ ID No. 1, and the amino acid sequence coded by the StPPO9 gene is as shown in SEQ ID No. 2. By overexpressing the StPPO9 gene in the plant (especially potato), the defensive reaction of the plant can be obviously activated, so that the resistance of the plant to late blight caused by phytophthora infestans is enhanced. The invention also provides a specific primer pair for cloning the gene, a protein coded by the gene, and application of a recombinant overexpression vector and engineering bacteria containing the gene in disease-resistant breeding. Effective gene resources and biotechnological means are provided for cultivation of potato resistant to late blight and new varieties of other solanaceae crops.

The invention discloses a small-molecule inhibitor of a targeted phytophthora sterol sensing receptor SSRK1 and application of the small-molecule inhibitor, and belongs to the technical field of pesticide biology. The inhibitor can specifically bind to a sterol binding site of SSRK1 and competitively block a natural sterol signal, so that comprehensive inhibition on growth, development and pathogenicity of phytophthora is realized. The compound shows remarkable bacteriostatic activity on various phytophthora (such as phytophthora sojae, phytophthora infestans and phytophthora capsici), and can effectively prevent and treat crop diseases such as soybean root rot, potato late blight and phytophthora capsici. The small-molecule inhibitor provided by the invention has the characteristics of novel target spot, unique action mechanism, broad spectrum, high efficiency, environmental friendliness and the like, is not easy to generate cross resistance with the traditional bactericide, and provides a new candidate molecule and preparation development approach for green prevention and control of phytophthora.

The present invention relates to a method for controlling or preventing an infestation of useful plants by a phytopathogenic microorganism selected from the group consisting of Fusarium pseudograminearum, Fusarium culmorum, Fusarium oxysporum, Fusarium graminearum, Bakania oryzae, Sporidesmium humicola, Botrytis cinerea, Phytophthora sojae, Phytophthora capsici, Phytophthora infestans, Pythium aphanidermatum, Pythium arrhenomanes, Pythium deliense, Puccinia striiformis, Puccinia hordei, Puccinia malvaccarum, Pseudoperonospora cubensis and Sphaerotheca fuliginea, which comprises the application of a compound of formula (I) to useful plants, to the locus thereof, or to their propagation material.

A method of controlling or preventing late blight caused by Phytophthora infestans in Solanum species comprising applying a composition comprising mandipropamid and propamocarb, or a salt thereof, to

The application belongs to the field of microorganism and genetic engineering technology, and particularly relates to a chitinase mutant and application thereof in promoting plant growth. The wild-type chitinase is subjected to directed evolution in vitro through error-prone PCR technology, and a chitinase mutant with significantly improved enzyme activity and environmental stability is obtained. The enzyme activity of the mutant at 50 DEG C and pH 7 is 1051.36 U / mL, which is about 2.7 times of that of the wild-type chitinase (385.45 U / mL). The mutant has good killing effect on pests such as pests of Lepidoptera, Thysanoptera and Coleoptera, and also has good bacteriostatic activity on pathogenic bacteria such as Pythium aphanidermatum, Pythium ultimum, Botrytis cinerea, Phytophthora infestans and Curvularia inaequalis. When used in the growth and development of vegetable plants or food plants, the mutant can promote plant growth and significantly improve the yield and quality of plants.

The invention discloses a plant immunomodulatory gene RP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, and it is verified that the plant immunomodulatory factor gene RP can be successfully expressed in other plants; according to the present invention, the disease resistance of the nicotiana benthamiana can be effectively improved after the expression of the gene, and the subsequent pathogenic effect verification of Phytophthora infestans, Phytophthora capsici and Phytophthora nicotiana proves that the disease resistance of the nicotiana benthamiana can be effectively improved after the expression of the gene, and the experimental result shows that the gene can be adopted as the candidate gene for cultivating the disease-resistant variety, and can be the theoretical support and the gene resource for preventing and controlling the plant diseases;

This invention discloses a plant immune-inducing protein, PsCAP1, and its applications. This protein can induce plant resistance and can be used as a plant immune activator in the control of plant diseases. The amino acid sequence of the protein is shown in SEQ ID NO.2. High concentrations of the protein can be obtained by expression using engineered strains. Treatment of plants with this protein can improve plant resistance to Phytophthora infestans, with low required concentrations, rapid onset of action, and long duration of action. PsCAP1 provides a new approach to improving plant disease resistance and controlling plant diseases.

The invention belongs to the field of polypeptide biopesticides, and provides a phytophthora infestans inhibitory peptide and application thereof, and the amino acid sequence of the phytophthora infestans inhibitory peptide is FKEGKAE or QVDNHLNSR. The phytophthora infestans inhibitory peptide has a remarkable inhibitory effect on phytophthora infestans mycelium and spore germination, and compared with traditional chemical pesticides, the inhibitory effect on the mycelium provides a new choice for prevention and treatment of phytophthora infestans related diseases such as potato late blight.

This invention discloses a method for identifying mutations in the PiORP1 gene of *Phytophthora infestans* and its resistance to fluthiazopyrone. The mutation site of the PiORP1 gene is an A / T heterozygous or T homozygous mutation at nucleotide position 2344 from the 5' end of the *Phytophthora infestans* gene. This results in mutations at position 757 of the PiORP1 protein, including heterozygous asparagine and isoleucine, heterozygous asparagine and leucine, heterozygous asparagine and phenylalanine, homozygous isoleucine, homozygous leucine, and homozygous phenylalanine. The molecular detection method provided by this invention has broad coverage, good detection stability, and a short detection cycle. It can be used for high-throughput detection of the occurrence and development of resistance genes to fluthiazopyrone in *Phytophthora infestans* in the field, enabling early warning of resistant strains, guiding scientific pesticide use, and delaying the development of resistance.