38 results about "Phytophthora infestans" patented technology

The invention discloses an ADF protein from phytophthora as well as a coding gene and application thereof. According to the present invention, the PsADF protein provided by the present invention is the protein represented by sequences 2 and 5 in Phytophthora sojae (Phytophthora sojae); the coding gene is shown as a sequence 3 and a sequence 4. Tests prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the 64-site mutation of the key amino acid functional site of the protein, the sporangium abnormality and zoospore quantity of the phytophthora sojae are reduced, and the pathogenicity is reduced. The protein provided by the invention has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and provides a molecular target for research and development of novel bactericides in the future. The corresponding ADF inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The protein provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention relates to application of mitogen-activated protein kinase PiPmk1 in screening molecules for inhibiting formation of a phytophthora infestans infection structure. The invention finds that the molecular marker plays a key role in the development of a phytophthora infestans cyst bud tube and / or the formation of an infection structure, which indicates that the molecular marker has the potential of serving as a target for inhibiting phytophthora infestans infection, and a corresponding molecular screening method and a generative prediction model are further developed and utilized; rapid and accurate prediction and screening of phytophthora infestans infection inhibitory molecules are realized, the research and development period and cost are greatly shortened, and a new thought and a new method are provided for development of accurate targeted green pesticides.

The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.

The invention discloses application of exogenous selenium to improvement of tomato late blight resistance, and belongs to the field of plant disease control. The exogenous selenium comprises Na2SeO3 (sodium selenite). Compared with a control group, exogenous selenium can inhibit the morbidity of tomato plants. Specifically, the exogenous selenium can inhibit the expansion of tomato leaf late blight scabs, relieve the accumulation of H2O2 and O2 <-> in the leaves and reduce the ROS content in the tomato leaves; meanwhile, the activity of antioxidant enzymes SOD, POD and CAT and the content of chlorophyll can be improved, and generation of MDA is reduced; besides, the content of SA and JA of tomato plants and the content of selenium in tomato leaves and fruits can also be increased by spraying exogenous selenium; in addition, through detection, exogenous selenium can improve the resistance of tomatoes to phytophthora infestans by inducing expression of the SIPR5 gene. In conclusion, the invention finds that the exogenous selenium can improve the resistance of the tomato to the late blight, and provides a new method for preventing and treating the tomato late blight.

The present invention relates to methods for controlling or preventing infestation of vegetable, tomato and potato plants by phytopathogenic microorganisms selected from Sphaerotheca fuliginea, Leveillula taurica, Sclerotinia sclerotiorum, Cercospora, Fusarium oxysporum, Fusarium solani, Helminthosporium solani, Phoma tuberosa, Rhizoctonia solani, Phytophthora infestans, Verticillium dahlia, Didymella bryoniae, Botrytis cinerea and Alternaria solani, comprising applying to a crop of plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The invention discloses an endophytic subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. The strain can inhibit the growth of different physiological races of phytophthora infestans, and has inhibitory activity on various pathogenic oomycetes. The fermentation liquor of the strain contains micromolecular antibacterial substances with relatively high stability, and has remarkable antibacterial activity; protein components in fermentation liquor can induce plants to generate resistance, and infection of phytophthora infestans is effectively inhibited. Different types of microbial agents prepared by utilizing the strain DS1 can be used for effectively preventing and treating potato late blight, and the microbial preparation is used for seed dressing treatment of potatoes and wheat and can be used for remarkably promoting the growth of crops.

The present invention relates to bio-fungicides that inhibits Phytophthora infestans. More particularly, the present invention relates to novel strains of Bacillus spp. that can inhibit Phytophthora infestans. The present invention also relates to bio-fungicidal formulations comprising novel strains of Bacillus spp. either alone or a mixture of novel strains of Bacillus spp., in combination with other excipients.

A method of controlling or preventing late blight caused by Phytophthora infestans in Solanum species comprising applying a composition comprising mandipropamid and propamocarb, or a salt thereof, to

The invention relates to a strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 and an application of the strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 in prevention and treatment of plant oomycetes diseases, and belongs to the field of research and development of microbial pesticides. The Brevibacillus formis LJQJ21 has the capabilities of promoting growth and preventing diseases, has a relatively strong inhibition effect on mycelial growth of various pathogenic oomycetes (phytophthora capsici, phytophthora infestans, peronophythora litchii and pythium aphanidermatum) causing crop diseases, and can secrete pentapeptide antibacterial substances Edeine A and B, and the Edeine can remarkably inhibit germination of zoospores of the phytophthora capsici. In addition, the microbial agent taking the LJQJ21 spore fermentation bacterial liquid as a main active component has a relatively good prevention and treatment effect on fruit rot caused by phytophthora capsici. The Brevibacillus forts LJQJ21 has obvious growth promoting and disease preventing effects, is safe to human and livestock and environment-friendly, and has potential field application value and development prospect.

The invention provides application of a DTX29 gene and a protein thereof in improving the resistance of plants to phytophthora infestans. The invention finds a susceptible gene DTX29 capable of effectively improving the resistance of plants to phytophthora infestans through a large number of researches. The DTX29 gene of the plant is edited through a CRISPR-Cas technology and the like, so that the function of the DTX29 gene is lost, the resistance of the plant to phytophthora infestans can be effectively improved, the diameter and area of disease spots and the relative biomass of phytophthora infestans are reduced, and normal growth and development of the plant are not influenced. The invention provides a new target and idea for prevention and control of plant diseases caused by phytophthora infestans.

The application discloses a preparation method and application of an indole oxadiazole aryl thiourea derivative, and has the structure shown in the following general formula: The compound has a good inhibiting effect on pathogenic bacteria, and has a good inhibiting effect on pathogenic bacteria such as Xanthomonas campestris, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. mori, Xanthomonas axonopodis pv. citri, Xanthomonas axonopodis pv. viticola, Xanthomonas axonopodis pv. vesicatoria, Xanthomonas axonopodis pv. actinidiae, Xanthomonas axonopodis pv. apodrecedens, Botrytis cinerea, Fusarium oxysporum f. sp. cubense, Sclerotinia sclerotiorum, Gibberella zeae, Phytophthora infestans, Monilinia fructicola, and the like, and provides an important scientific basis for research and development of new pesticides.

The application belongs to the technical field of molecular biology breeding, and particularly relates to application of RIN4 family genes in improving resistance of tomatoes to Phytophthora infestans. Two Phytophthora infestans disease-related genes are found and verified in tomatoes, the genomic nucleotide sequences of which are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes both belong to members of the RIN4 gene family. By knocking out or silencing the above genes through gene editing technology, it is found that the tomato lines with the above genes knocked out or silenced have higher resistance to Phytophthora infestans compared with wild types. Meanwhile, the gene-edited tomato lines are not obviously affected in growth and development. Therefore, the application not only enriches the research on Phytophthora infestans disease-related genes in tomatoes, but also develops tomato lines with enhanced resistance to Phytophthora infestans by using the characteristics of the above genes, thereby providing new technical strategies and gene resources for tomato breeding.

The invention discloses an NLR plug-in gene for improving plant disease resistance and application of the NLR plug-in gene, and belongs to the field of plant molecular biology and biotechnology. The NLR plug-in gene StEM1 has a complete gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein of the NLR plug-in gene StEM1 is as shown in SEQ ID NO.3. The gene plays a key role in improving the plant disease resistance, especially the resistance to field late blight variation flora. Under the background of NLR, the gene is overexpressed to remarkably promote the disease resistance of tobacco and potatoes to phytophthora infestans, and the gene is a gene for enhancing the plant disease resistance by improving ETI immunity. Through a genetic transformation method, a strain which overexpresses the gene under the background of NLR obtains resistance to field variant strains so as to cope with field diseases of crops. And excellent gene resources are provided for green prevention and control of late blight and breeding of disease-resistant gene engineering.

The present invention relates to bio-fungicides that inhibits Phytophthora infestans. More particularly, the present invention relates to novel strains of Bacillus spp. that can inhibit Phytophthora infestans. The present invention also relates to bio-fungicidal formulations comprising novel strains of Bacillus spp. either alone or a mixture of novel strains of Bacillus spp., in combination with other excipients.

The invention provides application of an SlSRC2 gene in regulation and control of tomato broad-spectrum disease resistance, AtSRC2 in arabidopsis thaliana is retrieved through NCBI (National Center of Biotechnology Information), BLAST analysis is performed in a tomato genome by using an amino acid sequence of the AtSRC2, and a protein, namely the SlSRC2, which has the highest similarity with the AtSRC2 in tomatoes is found. Through overexpression of the CDS region of the SlSRC2 in the tomato, the purpose of overexpression of the SlSRC2 is achieved, the alkaloid content in the tomato can be reduced, and the resistance to botrytis cinerea, phytophthora infestans and tomato yellow leaf curl virus is weakened; on the other hand, gene editing is conducted on the specific sequence of the SlSRC2 gene in the tomato by means of the CRISPR-Cas9 technology, the alkaloid content of the obtained mutant plant can be increased, and the resistance of the mutant plant to botrytis cinerea, phytophthora infestans and tomato yellow leaf curl virus is enhanced.

The invention provides a potato late blight biocontrol strain as well as a biocontrol inoculant and application thereof, and belongs to the technical field of biocontrol bacteria. The invention discloses a bacillus safeensis strain B1, and the biological preservation number of the bacillus safeensis strain B1 is CGMCC (China General Microbiological Culture Collection Center) No.36024. The invention also provides a biocontrol bacterium composition which is prepared by compounding the Leifsonia sp. Strain L1 alone or by combining the Leifsonia sp. Strain L1 with the Leifsonia sp. Strain L6 and the strain B1. The strain B1 has good activity of inhibiting phytophthora infestans, meanwhile, the biocontrol bacterium composition not only further improves the antibacterial activity of the strain B1, but also shows that the biocontrol bacterium composition can effectively reduce the disease index of potatoes, and compared with the single-phase strain B1, the biocontrol bacterium composition can effectively improve the prevention effect of potato late blight.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of PP2C family genes in improving resistance of tomatoes to phytophthora infestans. Two genes related to phytophthora infestans infection are found and verified in tomatoes, the genome nucleotide sequences of the two genes are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes belong to PP2C gene family members. The gene is knocked out or silenced through a gene editing technology, and it is found that the tomato strain with the gene knocked out or silenced has higher phytophthora infestans resistance compared with a wild type. Meanwhile, tomato strains edited by the genes are not obviously influenced in the aspects of growth and development. Therefore, the invention not only enriches the research of phytophthora infestans susceptibility related genes in tomatoes, but also develops a tomato strain with enhanced phytophthora infestans resistance by utilizing the characteristics of the genes, and provides a new technical strategy and gene resources for tomato breeding.

The present invention discloses the use of capillary diynone in combating tobacco phytophthora and Phytophthora infestans, belonging to the technical field of pesticides. The present invention discovers that capillary diynone can inhibit the growth of tobacco phytophthora and Phytophthora infestans, resisting attack by tobacco phytophthora, and can be used to prepare preparations for combating tobacco phytophthora and preventing tobacco black shank. Capillary diynone can also resist attack by Phytophthora infestans and can be used to prepare preparations for combating potato late blight, thus having significant significance for combating tobacco black shank and potato late blight.

The invention provides a biocontrol penicillium and application thereof, the biocontrol penicillium is Penicillium oxalicum POIL-1, the biocontrol penicillium is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.42328. The biocontrol penicillium has the advantages that the biocontrol penicillium is the Penicillium oxalicum POIL-1, and the biocontrol penicillium is the Penicillium oxalicum POIL-1; it is found for the first time that the strain or culture filtrate of the strain can effectively inhibit various common potato pathogenic bacteria including phytophthora infestans, can be used for field biological control of major diseases such as potato late blight and the like, and is beneficial to reduction of use of chemical pesticides. In addition, the strain significantly promotes the growth of solanaceae crops such as potatoes, tobaccos and tomatoes, has good phosphate solubilizing ability, and is helpful for improving the crop yield and reducing the use of phosphate fertilizer. The Penicillium oxalicum POIL-1 strain can be used for biological pesticide development, has triple effects of preventing diseases, promoting growth and improving soil quality, is green and environment-friendly, is low in cost, and has good application potential.

This invention relates to the field of genetic engineering technology, and particularly to soybean GmST3 protein mutants and their application in regulating soybean resistance to Phytophthora infestans. This invention reveals for the first time that the soybean GmST3 protein and its encoding gene have the function of regulating soybean resistance to Phytophthora infestans. By reducing the expression of wild-type soybean GmST3 protein or its encoding gene, or by inducing soybean plants to express soybean GmST3 protein mutants, soybean resistance to Phytophthora infestans can be effectively improved. The soybean GmST3 protein mutants and their encoding genes provided by this invention can also be used for the breeding of soybean Phytophthora-resistant varieties, and have broad application prospects.

The application discloses a kind of polyphenol oxidase StPPO9 The application discloses application of a gene in improving plant resistance to late blight, and belongs to the field of plant genetic engineering. StPPO9 The nucleotide sequence of the gene is shown as SEQ ID No.1, and the amino acid sequence encoded by the gene is shown as SEQ ID No.2. StPPO9 By overexpressing the gene in plants (especially potatoes), the application can significantly activate the defense response of the plants, thereby enhancing the resistance of the plants to late blight caused by Phytophthora infestans. The application also provides a specific primer pair for cloning the gene, a protein encoded by the gene, and application of a recombinant overexpression vector containing the gene and an engineering bacterium in disease-resistant breeding. The application provides an effective gene resource and a biotechnological means for cultivating new potato and other solanaceous crop varieties resistant to late blight.

The invention discloses an application of selenomethionine in any one of the following applications: (1) an application in inhibition of Phytophthora infestans (Phytophthora infestans) or pathogenic fungi, and (2) an application in inhibition of the Phytophthora infestans (Phytophthora infestans); (2) application in preparation of products for inhibiting phytophthora infestans or pathogenic fungi; (3) application in prevention and treatment of plant late blight or diseases caused by pathogenic fungi; (4) application in preparation of products for preventing and treating plant late blight or diseases caused by pathogenic fungi; (5) application in plant growth promotion; and (6) application in preparation of the plant growth promoter. The invention provides a novel green pesticide product and novel application of selenomethionine in prevention and treatment of pathogenic bacteria and related plant diseases. A novel green pesticide is provided for plant late blight, and the use of chemical pesticides can be reduced.

The application belongs to the technical field of molecular biology breeding, and particularly relates to application of EF Hand family genes in improving resistance of tomatoes to pathogenic Phytophthora. Two pathogenic Phytophthora disease-related genes are found and verified in tomatoes, the genomic nucleotide sequences of which are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes both belong to members of the EF Hand gene family. By knocking out or silencing the above genes through gene editing technology, it is found that the tomato lines with the above genes knocked out or silenced have higher resistance to pathogenic Phytophthora compared with wild types. Meanwhile, the gene-edited tomato lines are not obviously affected in growth and development. Therefore, the application not only enriches the research on pathogenic Phytophthora disease-related genes in tomatoes, but also develops tomato lines with enhanced resistance to pathogenic Phytophthora by using the characteristics of the above genes, thereby providing new technical strategies and gene resources for tomato breeding.

The present invention provides two strains of endophytic fungi Epichloe bromicola from Elymus tangutorum, with the preservation numbers being CGMCC No. 22448 and CGMCC No. 22449 respectively. The applicant for the first time used the plate mycelium inhibition method to test the inhibitory activities of crude extracts of different endophytic fungi from Elymus tangutorum against Phytophthora infestans. Through the activity inhibition experiment, the inhibition rates of the endophytic fungal strains 1 and 8 from Elymus tangutorum against Phytophthora infestans were strong; these two strains were preserved. In addition, the applicant tested the crude extracts of the endophytic fungal strains 1 and 8 from Elymus tangutorum for their control effects on potato late blight through in vitro leaf experiments. And the crude extracts of these two endophytic fungi may have low toxicity. Therefore, the endophytic fungal strains 1 and 8 from Elymus tangutorum can be used as promising plant-derived fungicides.

The invention discloses application of a polyphenol oxidase StPPO9 gene to improvement of late blight resistance of plants, and belongs to the field of plant genetic engineering. The nucleotide sequence of the StPPO9 gene is as shown in SEQ ID No. 1, and the amino acid sequence coded by the StPPO9 gene is as shown in SEQ ID No. 2. By overexpressing the StPPO9 gene in the plant (especially potato), the defensive reaction of the plant can be obviously activated, so that the resistance of the plant to late blight caused by phytophthora infestans is enhanced. The invention also provides a specific primer pair for cloning the gene, a protein coded by the gene, and application of a recombinant overexpression vector and engineering bacteria containing the gene in disease-resistant breeding. Effective gene resources and biotechnological means are provided for cultivation of potato resistant to late blight and new varieties of other solanaceae crops.

The present invention discloses a molecular marker primer and a method for identifying Phytophthora infestans of potato late blight. The molecular marker primer is used to identify Phytophthora infestans causing potato late blight, and the molecular markers are as shown in SEQ ID NO: 1-6 in the sequence listing. The identification method comprises the following steps: extracting the tissue DNA of the material to be tested; performing PCR amplification using the shown molecular marker primer; and detecting the PCR amplification product by 1.5% agarose gel electrophoresis. The three pairs of primers provided by the present invention can only obtain positive amplification bands when the DNA of Phytophthora infestans is used as a template. The similarities of the amplification products with the expected sequences are 94.96%, 97.71%, and 96.44% respectively after sequencing analysis, while no cloning bands are obtained when the genomic DNA of related species such as Phytophthora capsici is used as a template. The primers have good specificity and can effectively distinguish related species. The method of the present invention can detect leaves that have been infected with the bacteria but have no lesions, which is quite important for disease prevention and control.

The invention discloses a small-molecule inhibitor of a targeted phytophthora sterol sensing receptor SSRK1 and application of the small-molecule inhibitor, and belongs to the technical field of pesticide biology. The inhibitor can specifically bind to a sterol binding site of SSRK1 and competitively block a natural sterol signal, so that comprehensive inhibition on growth, development and pathogenicity of phytophthora is realized. The compound shows remarkable bacteriostatic activity on various phytophthora (such as phytophthora sojae, phytophthora infestans and phytophthora capsici), and can effectively prevent and treat crop diseases such as soybean root rot, potato late blight and phytophthora capsici. The small-molecule inhibitor provided by the invention has the characteristics of novel target spot, unique action mechanism, broad spectrum, high efficiency, environmental friendliness and the like, is not easy to generate cross resistance with the traditional bactericide, and provides a new candidate molecule and preparation development approach for green prevention and control of phytophthora.

The present invention relates to a method for controlling or preventing an infestation of useful plants by a phytopathogenic microorganism selected from the group consisting of Fusarium pseudograminearum, Fusarium culmorum, Fusarium oxysporum, Fusarium graminearum, Bakania oryzae, Sporidesmium humicola, Botrytis cinerea, Phytophthora sojae, Phytophthora capsici, Phytophthora infestans, Pythium aphanidermatum, Pythium arrhenomanes, Pythium deliense, Puccinia striiformis, Puccinia hordei, Puccinia malvaccarum, Pseudoperonospora cubensis and Sphaerotheca fuliginea, which comprises the application of a compound of formula (I) to useful plants, to the locus thereof, or to their propagation material.

The invention relates to the technical field of plant pathology detection, in particular to a potato late blight molecular identification method of a molecular marker primer, and the primer comprises a molecular marker primer pair for identification of Phytophthora infestans (Phytophthora infestans). The primer pair comprises specific sequences of three continuous adenine bases (AAA) in a targeted pathogenic bacterium ribosome internal transcriptional spacer (ITS), and comprises a forward primer, a reverse primer, a reverse primer and a reverse primer, the nucleotide sequence of the forward primer is shown as SEQ ID NO: 1, and the nucleotide sequence of the reverse primer is 5 '-GCTGCGTCTTCTATGATGC-3'; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO: 2: 5 '-TCCTCCGCTTATTGATATGC-3'. The nucleotide sequence of the reverse primer is as shown in SEQ ID NO: 2. According to the potato late blight molecular identification method of the molecular marker primer, 100% specificity of the primer pair is achieved through base mismatch design, detection of samples such as tubers and soil can be completed within 4 hours by combining optimized CTAB / magnetic bead method extraction and 56 DEG C annealing PCR technology, the sensitivity reaches 10 pg / mu L, and the primer pair is high in specificity and high in specificity. The method is suitable for early warning and accurate prevention and control of potato late blight.

A method of controlling or preventing late blight caused by Phytophthora infestans in Solanum species comprising applying a composition comprising mandipropamid and propamocarb, or a salt thereof, to