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Biocontrol inoculant for controlling potato late blight

A technology for potato late blight and biocontrol agent, which is applied in the directions of biocides, fungicides, biocides, etc., can solve problems such as unsatisfactory chemical control effect and environmental pollution, achieve good development and application prospects, reduce farmland pollution, The effect of easy industrial production

Active Publication Date: 2017-09-08
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a biocontrol agent for preventing and treating potato late blight in view of the serious occurrence of potato late blight at present, and the area is expanding year by year, the effect of chemical control is not ideal, and environmental pollution is caused.

Method used

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  • Biocontrol inoculant for controlling potato late blight
  • Biocontrol inoculant for controlling potato late blight
  • Biocontrol inoculant for controlling potato late blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Isolation of Antagonistic Actinomycetes

[0026] The specific process is as follows:

[0027] 1. Collection of soil samples

[0028] The rhizosphere soil at a depth of 5-10 cm from different potato plots was collected from Zhouning County, Fujian Province, divided into packages, labeled and brought back to the laboratory, where they were separated after natural air-drying.

[0029] 2. Isolation of actinomycetes

[0030] Separation was performed using the plate dilution method. Weigh 10 g of the soil sample, pour it into a conical flask filled with small glass beads and 90 ml of sterile water, shake it for 30 min, then let it stand for 5 min, dilute it by 10 times successively, and prepare 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Suspension of different concentrations, each 0.1 ml of the suspension was drawn and added to Gao's No. 1 medium (add K with a final concentration of 50 mg / L 2 Cr 2 o 7 ) plate, spread evenly and culture at 28°C for observation, and ...

Embodiment 2

[0031] Identification of embodiment two bacterial strain St19

[0032] 1. Observation of morphological characteristics

[0033] Strain St19 was inoculated on Gao's No. 1 medium, cultured at 28 °C for 10 days, and the morphology of the colonies was observed ( figure 1 ), and at the same time, insert a sterile cover glass obliquely at 45°C. After culturing at 28°C for 7 days, take out the cover glass and observe the morphological characteristics of the hyphae under an optical microscope and an electron microscope ( figure 2 and image 3 ).

[0034] 2. Observation of culture characteristics

[0035] The 8 kinds of media recommended by the "Streptomyces Identification Manual" were used to culture the strain St19 at 28 °C for 7-10 days, then observe the growth of the bacteria, and record the color of the aerial hyphae and basal hyphae and whether soluble pigments were produced. Strain St19 grew well on Gao's No. 1 medium, Chabek medium, starch ammonium medium, PDA medium, glu...

Embodiment 3

[0040] The optimization of embodiment three St19 bacterial strain fermentation technology

[0041] The above-mentioned strains were inoculated in Gaoshi No. 1 liquid medium, cultured at 28 °C, 150 r / min for 5 days, then made into seed liquid, and inoculated in the fermentation medium. Taking Potato infestans as the target bacteria, through the screening of carbon source and nitrogen source, single factor test and orthogonal test of nutritional conditions, the optimal fermentation medium formula of this strain was obtained: soluble starch 2% wt, glucose 1.5 % wt, soybean flour 2% wt, MgSO 4 ·7H 2 O 0.05% wt, K 2 HPO 4 0.1% wt, NaCl 0.1% wt, CaCO 30.2% wt, the rest is distilled water. The optimal fermentation conditions were: inoculum volume 1%-2% vol, bottle volume 100mL / 250mL, initial pH value of fermentation medium 7.0-7.2, 28 ℃, 150 r / min, fermentation time 9 days. After the fermentation broth was centrifuged at 4000 r / min for 5 min, it was sterilized by filtration wi...

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Abstract

The invention discloses a biocontrol inoculant for controlling potato late blight, and belongs to the technical field of crop disease control. The biocontrol inoculant is fermented by streptomyceseuropaeiscabiei St19. A strain is registered and preserved in China General Microbiological Culture Collection Center (CGMCC) on March 24, 2014; and the preservation number is CGMCCNO.8962. The biocontrol inoculant can inhibit growth of potato phytophthora infestans, represents a better control effect on the potato late blight, can be used for root irrigation in a seedling stage or an adult-plant stage of a potato, or can be mixed with an organic fertilizer to prepare a microbial fertilizer. The St19 strain is obtained from potato rhizosphere soil, is harmonious and compatible with soil ecology, and is non-toxic and non-virulent, so that the strain has a larger application potential in biological control of the potato late blight.

Description

technical field [0001] The invention relates to a biocontrol bacterial agent for preventing and treating potato late blight, and belongs to the technical field of crop disease prevention and control. Background technique [0002] by Phytophthora infestans ( Phytophthora infestans Late blight caused by (Mont) de Bary) is a devastating disease in potato production. It occurs and is prevalent in all major potato producing areas in the world, and seriously threatens potato production. The damage it causes, the difficulty of control and the The social impact has surpassed rice blast and wheat stripe rust, and is regarded as the largest crop disease in the world. Late blight is common and serious in our country, causing an economic loss of about 8 billion yuan each year due to late blight, which has become the first major obstacle to restricting potato production and industrialization in my country. [0003] So far, the prevention and control of late blight mainly relies on the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00
CPCA01N63/30
Inventor 李本金陈庆河刘裴清王荣波翁启勇
Owner INST OF PLANT PROTECTION FAAS
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